scholarly journals Proteomic characterisation of serum proteins from Atlantic salmon (Salmo salar L.) from an outbreak with Cardiomyopathy Syndrome

2021 ◽  
Author(s):  
Janina Costa ◽  
Jorge del Pozo ◽  
Kevin McLean ◽  
Neil Inglis ◽  
Philippe Sourd ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Glycogen Phosphorylase ◽  
Serum Proteins ◽  
Diagnostic Methods ◽  
Diagnostic Tools ◽  
Host Reaction ◽  
Α2 Macroglobulin ◽  
Prophylactic Measures ◽  
Related Proteins

Abstract Cardiomyopathy syndrome (CMS), caused by piscine myocarditis virus (PMCV), is a serious challenge to Atlantic salmon (Salmo salar L.) aquaculture. Regrettably, husbandry techniques are the only tool to manage CMS outbreaks, and no prophylactic measures are available at present. Early diagnosis of CMS is therefore desirable, preferably with non-lethal diagnostic methods, such as serum biomarkers. To identify candidate biomarkers for CMS, the protein content of pools of sera (4 fish/pool) from salmon a CMS outbreak (3 pools) and from clinically healthy salmon (3 pools) were compared using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Overall, seven proteins were uniquely identified in the sera of clinically healthy fish, while twenty seven proteins were unique to the sera of CMS fish. Of the latter, 24 have been associated with cardiac disease in humans. These were grouped as leakage enzymes (creatine kinase, lactate dehydrogenase, glycogen phosphorylase and carbonic anhydrase); host reaction proteins (acute phase response proteins - haptoglobin, fibrinogen, α2-macroglobulin, ceruloplasmin; and complement-related proteins); and regeneration/remodelling proteins (fibronectin, lumican and retinol). Clinical evaluation of the suitability of these proteins as biomarkers of CMS, either individually or as part of a panel, is a logical next step for the development of early diagnostic tools for CMS.

scholarly journals Proteomic characterisation of serum proteins from Atlantic salmon (Salmo salar L.) from an outbreak with Cardiomyopathy Syndrome

2021 ◽  
Author(s):  
Janina Costa ◽  
Jorge del Pozo ◽  
Kim Thompson ◽  
Kevin McLean ◽  
Neil Inglis ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Glycogen Phosphorylase ◽  
Serum Proteins ◽  
Diagnostic Methods ◽  
Diagnostic Tools ◽  
Host Reaction ◽  
Α2 Macroglobulin ◽  
Prophylactic Measures ◽  
Related Proteins

Abstract Cardiomyopathy syndrome (CMS), caused by piscine myocarditis virus (PMCV), is a serious challenge to Atlantic salmon (Salmo salar L.) aquaculture. Regrettably, husbandry techniques are the only tool to manage CMS outbreaks, and no prophylactic measures are available at present. Early diagnosis of CMS is therefore desirable, preferably with non-lethal diagnostic methods, such as serum biomarkers. To identify candidate biomarkers for CMS, the protein content of pools of sera (4 fish/pool) from salmon a CMS outbreak (3 pools) and from clinically healthy salmon (3 pools) were compared using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Overall, seven proteins were uniquely identified in the sera of clinically healthy fish, while twenty seven proteins were unique to the sera of CMS fish. Of the latter, 24 have been associated with cardiac disease in humans. These were grouped as leakage enzymes (creatine kinase, lactate dehydrogenase, glycogen phosphorylase and carbonic anhydrase); host reaction proteins (acute phase response proteins - haptoglobin, fibrinogen, α2-macroglobulin, ceruloplasmin; and complement-related proteins); and regeneration/remodelling proteins (fibronectin, lumican and retinol). Clinical evaluation of the suitability of these proteins as biomarkers of CMS, either individually or as part of a panel, is a logical next step for the development of early diagnostic tools for CMS.

scholarly journals Proteomic Characterisation of Serum Proteins From Atlantic Salmon (Salmo salar L.) With Cardiomyopathy Syndrome (CMS)

2021 ◽  
Author(s):  
Janina Costa ◽  
Kim Thompson ◽  
Jorge del Pozo ◽  
Kevin McLean ◽  
Neil Inglis ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Glycogen Phosphorylase ◽  
Serum Proteins ◽  
Diagnostic Methods ◽  
Diagnostic Tools ◽  
Host Reaction ◽  
Α2 Macroglobulin ◽  
Prophylactic Measures ◽  
Related Proteins

Abstract Cardiomyopathy syndrome (CMS), caused by piscine myocarditis virus (PMCV), is a serious challenge to Atlantic salmon (Salmo salar L.) aquaculture. Regrettably, husbandry techniques are the only tool to manage CMS outbreaks, and no prophylactic measures are available at present. Early diagnosis of CMS is therefore desirable, preferably with non-lethal diagnostic methods, such as serum biomarkers. To identify candidate biomarkers for CMS, the protein content of pools of sera (4 fish/pool) from salmon with CMS (4 pools) and from clinically healthy salmon (3 pools) were compared using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Overall, seven proteins were uniquely identified in the sera of clinically healthy salmon, while twenty seven proteins were unique to the sera of CMS salmon. Of the latter, 24 have been associated with cardiac disease in humans. These were grouped as leakage enzymes (creatine kinase, lactate dehydrogenase, glycogen phosphorylase and carbonic anhydrase); host reaction proteins (acute phase response proteins - haptoglobin, fibrinogen, α2-macroglobulin, ceruloplasmin; and complement-related proteins); and regeneration/remodelling proteins (fibronectin, lumican and retinol). Clinical evaluation of the suitability of these proteins as biomarkers of CMS, either individually or as part of a panel, is a logical next step for the development of early diagnostic tools for CMS.

scholarly journals Proteomic characterisation of serum proteins from Atlantic salmon (Salmo salar L.) from an outbreak with Cardiomyopathy Syndrome

2021 ◽  
Author(s):  
Janina Costa ◽  
Kim Thompson ◽  
Jorge del Pozo ◽  
Kevin McLean ◽  
Neil Inglis ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Glycogen Phosphorylase ◽  
Serum Proteins ◽  
Diagnostic Methods ◽  
Diagnostic Tools ◽  
Host Reaction ◽  
Α2 Macroglobulin ◽  
Prophylactic Measures ◽  
Related Proteins

Abstract Cardiomyopathy syndrome (CMS), caused by piscine myocarditis virus (PMCV), is a serious challenge to Atlantic salmon (Salmo salar L.) aquaculture. Regrettably, husbandry techniques are the only tool to manage CMS outbreaks, and no prophylactic measures are available at present. Early diagnosis of CMS is therefore desirable, preferably with non-lethal diagnostic methods, such as serum biomarkers. To identify candidate biomarkers for CMS, the protein content of pools of sera (4 fish/pool) from salmon a CMS outbreak (3 pools) and from clinically healthy salmon (3 pools) were compared using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Overall, seven proteins were uniquely identified in the sera of clinically healthy fish, while twenty seven proteins were unique to the sera of CMS fish. Of the latter, 24 have been associated with cardiac disease in humans. These were grouped as leakage enzymes (creatine kinase, lactate dehydrogenase, glycogen phosphorylase and carbonic anhydrase); host reaction proteins (acute phase response proteins - haptoglobin, fibrinogen, α2-macroglobulin, ceruloplasmin; and complement-related proteins); and regeneration/remodelling proteins (fibronectin, lumican and retinol). Clinical evaluation of the suitability of these proteins as biomarkers of CMS, either individually or as part of a panel, is a logical next step for the development of early diagnostic tools for CMS.

Differences in Atlantic Salmon, Salmo salar, from North America and Europe

1972 ◽  
Vol 29 (2) ◽  
pp. 179-185 ◽  
Author(s):  
O. L. Nyman ◽  
J. H. C. Pippy
Keyword(s):  
North America ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
North American ◽  
Serum Proteins ◽  
Fork Length ◽  
Anisakis Simplex ◽  
Atlantic Salmon Salmo Salar

Differences in electropherograms produced by serum proteins and liver esterases were used to identify North American and European Atlantic salmon (Salmo salar) caught at sea. Division of salmon according to continent of origin was supported by mean river age, mean fork length, and abundance of the two parasites Anisakis simplex and Eubothrium crassum. Consistent differences in electrophoretic behaviour of serum proteins and liver esterases in salmon from the two continents support the suggestion that salmon from North America and Europe represent different subspecies.

scholarly journals Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar)

2021 ◽  
Vol 12 ◽  
Author(s):  
Juan Pablo Pontigo ◽  
Carla Espinoza ◽  
Mauricio Hernandez ◽  
Guillermo Nourdin ◽  
Cristian Oliver ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Immune Responses ◽  
Salmo Salar ◽  
Protein Identification ◽  
Economic Losses ◽  
Prophylactic Measures ◽  
Membrane Compartment ◽  
Protective Antibodies ◽  
Chilean Isolate ◽  
Piscirickettsia Salmonis

An effective and economical vaccine against the Piscirickettsia salmonis pathogen is needed for sustainable salmon farming and to reduce disease-related economic losses. Consequently, the aquaculture industry urgently needs to investigate efficient prophylactic measures. Three protein-based vaccine prototypes against Piscirickettsia salmonis were prepared from a highly pathogenic Chilean isolate. Only one vaccine effectively protected Atlantic salmon (Salmo salar), in correlation with the induction of Piscirickettsia-specific IgM antibodies and a high induction of transcripts encoding pro-inflammatory cytokines (i.e., Il-1β and TNF-α). In addition, we studied the proteome fraction protein of P. salmonis strain Austral-005 using multidimensional protein identification technology. The analyzes identified 87 proteins of different subcellular origins, such as the cytoplasmic and membrane compartment, where many of them have virulence functions. The other two prototypes activated only the innate immune responses, but did not protect Salmo salar against P. salmonis. These results suggest that the knowledge of the formulation of vaccines based on P. salmonis proteins is useful as an effective therapy, this demonstrates the importance of the different research tools to improve the study of the different immune responses, resistance to diseases in the Atlantic salmon. We suggest that this vaccine can help prevent widespread infection by P. salmonis, in addition to being able to be used as a booster after a primary vaccine to maintain high levels of circulating protective antibodies, greatly helping to reduce the economic losses caused by the pathogen.

scholarly journals Proteomic characterization of serum proteins from Atlantic salmon ( Salmo salar L.) from an outbreak with cardiomyopathy syndrome

2021 ◽  
Author(s):  
Janina Z. Costa ◽  
Jorge Pozo ◽  
Kevin McLean ◽  
Neil Inglis ◽  
Philippe Sourd ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Serum Proteins ◽  
Atlantic Salmon Salmo Salar

Microstructure and texture of fresh and smoked Atlantic salmon, Salmo salar L., fillets from fish reared and slaughtered under different conditions

2001 ◽  
Vol 32 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Sjofn Sigurgisladottir ◽  
Margret S. Sigurdardottir ◽  
Helga Ingvarsdottir ◽  
Ole J. Torrissen ◽  
Hannes Hafsteinsson
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Atlantic Salmon Salmo Salar

Persistence of Orally Administered Salmonella enterica Serovars Agona and Montevideo in Atlantic Salmon (Salmo salar L.)

2005 ◽  
Vol 68 (7) ◽  
pp. 1336-1339 ◽  
Author(s):  
L. L. NESSE ◽  
T. LØVOLD ◽  
B. BERGSJØ ◽  
K. NORDBY ◽  
C. WALLACE ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Salmonella Enterica ◽  
Most Probable Number ◽  
Fish Feed ◽  
Internal Organs ◽  
Probable Number ◽  
Atlantic Salmon Salmo Salar ◽  
Gastric Intubation

The objective of our experiments was to study the persistence and dissemination of orally administered Salmonella in smoltified Atlantic salmon. In experiment 1, salmon kept at 15°C were fed for 1 week with feed contaminated with 96 most-probable-number units of Salmonella Agona per 100 g of feed and then starved for 2 weeks. Samples were taken from the gastrointestinal tract and examined for Salmonella 1, 2, 8, 9, 15, and 16 days after the feeding ended. In experiment 2, Salmonella Agona and Montevideo were separately mixed with feed and administered by gastric intubation. Each fish received 1.0 × 108, 1.0 × 106, or 1.0 × 104 CFU. The different groups were kept in parallel at 5 and 15°C and observed for 4 weeks. Every week, three fish in each group were sacrificed, and samples were taken from the skin, the pooled internal organs, the muscle, and the gastrointestinal tract and examined for the presence of Salmonella. The results from the two experiments showed that the persistence of Salmonella in the fish was highly dependent on the dose administered. Salmonella was not recovered from any of the fish that were fed for 1 week with the lowest concentration of Salmonella. In the fish given the highest dose of Salmonella, bacteria persisted for at least 4 weeks in the gastrointestinal tract as well as, to some extent, the internal organs. The present study shows that under practical conditions in Norway, the risk of Salmonella in fish feed being passed on to the consumer of the fish is negligible.

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