scholarly journals Quantitative Proteomics Reveals the Regulatory Networks of Circular RNA BTBD7_hsa_circ_000563 in human coronary artery

2020 ◽  
Author(s):  
Jia-Xin Chen ◽  
Lei Hua ◽  
Chen-Hui Zhao ◽  
Qiao-Wei Jia ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Regulatory Networks ◽  
Circular Rna ◽  
Mechanism Study ◽  
Human Coronary Artery ◽  
Atherosclerotic Stenosis ◽  
Coronary Artery Atherosclerosis ◽  
Underlying Mechanisms ◽  
And Function ◽  
Atherosclerotic Changes

Abstract Background: To investigate the association of BTBD7_hsa_circ_000563 expression in coronary artery segments with atherosclerotic stenosis, and to explore the proteome-wide identification of the BTBD7_hsa_circ_000563-regulated proteins in coronary artery Methods: The coronary artery samples were obtained from two autopsy cases. The epicardial coronary artery of every autopsy was divided into 10 segments, and coronary atherosclerosis grade and extent of the coronary artery segments were analysed by Haematoxylin and Eosin (H&E) staining. The BTBD7_hsa_circ_000563 expression of 8 segments from case 2 was quantified using RT-qPCR analysis. Results: The present study demonstrated that coronary artery segments with severe atherosclerotic stenosis showed extremely low expression of the BTBD7_hsa_circ_000563, compared with normal coronary artery segments. Furthermore, it was predicted that hsa-miR-155-5p, and hsa-miR-130a-3p are targets of the BTBD7_hsa_circ_000563. The results from the present study may laid an epigenetic foundation for studying the underlying mechanisms of the development and progression of coronary artery atherosclerosis. Conclusions: BTBD7_hsa_circ_000563 were involved in atherosclerotic changes in coronary artery segments of human being, and the verification study, mechanism study, and function study are necessary in order for CAD patients to benefit from the personalized medicine in the future.

scholarly journals Quantitative proteomics reveals the regulatory networks of circular RNA BTBD7_hsa_circ_0000563 in human coronary artery

2020 ◽  
Vol 34 (11) ◽  
Author(s):  
Jia‐Xin Chen ◽  
Lei Hua ◽  
Chen‐Hui Zhao ◽  
Qiao‐Wei Jia ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Quantitative Proteomics ◽  
Regulatory Networks ◽  
Circular Rna ◽  
Human Coronary Artery

scholarly journals MPST but not CSE is the primary regulator of hydrogen sulfide production and function in the coronary artery

2016 ◽  
Vol 310 (1) ◽  
pp. H71-H79 ◽  
Author(s):  
Maggie M. Kuo ◽  
Dae Hee Kim ◽  
Sandeep Jandu ◽  
Yehudit Bergman ◽  
Siqi Tan ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Coronary Artery ◽  
Ex Vivo ◽  
Human Coronary Artery ◽  
Sodium Hydrosulfide ◽  
Physiological Relevance ◽  
Hydrogen Sulfide Production ◽  
And Function

Hydrogen sulfide (H2S) has emerged as an important gasotransmitter in the vasculature. In this study, we tested the hypothesis that H2S contributes to coronary vasoregulation and evaluated the physiological relevance of two sources of H2S, namely, cystathionine-γ-lyase (CSE) and 3-mercaptypyruvate sulfertransferase (MPST). MPST was detected in human coronary artery endothelial cells as well as rat and mouse coronary artery; CSE was not detected in the coronary vasculature. Rat coronary artery homogenates produced H2S through the MPST pathway but not the CSE pathway in vitro. In vivo coronary vasorelaxation response was similar in CSE knockout mice, wild-type mice (WT), and WT mice treated with the CSE inhibitor propargylglycine, suggesting that CSE-produced H2S does not have a significant role in coronary vasoregulation in vivo. Ex vivo, the MPST substrate 3-mercaptopyruvate (3-MP) and H2S donor sodium hydrosulfide (NaHS) elicited similar coronary vasoreactivity responses. Pyruvate did not have any effects on vasoreactivity. The vasoactive effect of H2S appeared to be nitric oxide (NO) dependent: H2S induced coronary vasoconstriction in the presence of NO and vasorelaxation in its absence. Maximal endothelial-dependent relaxation was intact after 3-MP and NaHS induced an increase in preconstriction tone, suggesting that endothelial NO synthase activity was not significantly inhibited. In vitro, H2S reacted with NO, which may, in part explain the vasoconstrictive effects of 3-MP and NaHS. Taken together, these data show that MPST rather than CSE generates H2S in coronary artery, mediating its effects through direct modulation of NO. This has important implications for H2S-based therapy in healthy and diseased coronary arteries.

The Landscape of Proteomic Characteristics of Human Coronary Artery Atherosclerosis

2019 ◽  
Author(s):  
Jinxia Yuan ◽  
Yinwen Fan ◽  
Fenghui An ◽  
Jiaxin Chen ◽  
Yongjie Zhang ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Human Coronary Artery ◽  
Coronary Artery Atherosclerosis

scholarly journals A Highly Conserved Circular RNA circRasGEF1B Enhances Antiviral Immunity by Regulating miR-21-3p/MITA Pathway in Lower Vertebrates

2021 ◽  
Author(s):  
Qing Chu ◽  
Weiwei Zheng ◽  
Hui Su ◽  
Lei Zhang ◽  
Renjie Chang ◽  
...  
Keyword(s):  
Teleost Fish ◽  
Regulatory Networks ◽  
Antiviral Immunity ◽  
Circular Rna ◽  
Circular Rnas ◽  
Siniperca Chuatsi ◽  
Antiviral Responses ◽  
Repressive Effect ◽  
Lower Vertebrates ◽  
And Function

Circular RNAs (circRNAs) represent a class of widespread, diverse, and covalently closed circRNAs that function as microRNA (miRNA) sponges and crucial regulators of gene expression in mammals. However, the regulation and function of circRNAs in lower vertebrates are still unknown. Here, we first discover a highly conserved circRNA termed circRasGEF1B, which displays a high conservation from mammals to fish and serves as key regulator in eliciting antiviral immunity in teleost fish. Results indicate that circRasGEF1B was highly expressed in Siniperca chuatsi rhabdovirus-infected tissues and cells. Functionally, miR-21-3p could inhibit cellular antiviral responses significantly, whereas circRasGEF1B counteract the effects of miR-21-3p. In mechanism, the results demonstrate that circRasGEF1B acts as a competing endogenous RNA (ceRNA) of miR-21-3p to relieve the repressive effect of miR-21-3p on its target MITA, then enhance the innate antiviral responses. Our results not only provide a novel insight into the functions of circRNAs in lower vertebrates, but broaden our understanding of circRNAs in viral infection. IMPORTANCE Siniperca chuatsi rhabdovirus (SCRV) is a typical fish RNA rhabdovirus, which is one of the most significant viral pathogens in teleost fish and can cause severe hemorrhagic septicemia in freshwater and marine fishes. Here, we discovered a highly conserved circRNAs called circRasGEF1B, which acts as a key regulator for innate antiviral responses upon SCRV infection. circRasGEF1B acts as an endogenous sponge of miR-21-3p that downregulates miR-21-3p expression levels. circRasGEF1B is able to bind to miR-21-3p directly and regulates MITA expression. To our knowledge, this report is the first to characterize circRNA–miRNA regulatory networks that exist in lower vertebrates.

scholarly journals Proteomic landscape of human coronary artery atherosclerosis

2020 ◽  
Vol 46 (1) ◽  
pp. 371-383 ◽  
Author(s):  
Yaqing Zhou ◽  
Jinxia Yuan ◽  
Yinwen Fan ◽  
Fenghui An ◽  
Jiaxin Chen ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Human Coronary Artery ◽  
Coronary Artery Atherosclerosis
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