scholarly journals An Adult Plant Stripe Rust Resistance Gene Maps on Chromosome 7A of Australian Wheat Cultivar Axe

2021 ◽  
Author(s):  
Mehwish Kanwal ◽  
Naeela Qureshi ◽  
Mesfin Gessese ◽  
Kerrie Forrest ◽  
Prashanth Babu ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Wheat Cultivar ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Growth Stages ◽  
Resistance Locus ◽  
Leaf Stage ◽  
Ril Population ◽  
Australian Wheat

Abstract Australian wheat cultivar Axe produced resistant to moderately resistant stripe rust responses under field conditions and was exhibiting seedling response varying from 33C to 3+ under greenhouse conditions. Experiments covering tests at different growth stages (2nd, 3rd and 4th leaf stages) demonstrated the clear expression of resistance at the 4th leaf stage under controlled-environment greenhouse conditions. A recombinant inbred line (RIL) population was developed from the Axe/Nyabing-3 (Nyb) cross. Genetic analysis of Axe/Nyb RIL population in the greenhouse at the 4th leaf stage showed monogenic inheritance of stripe rust resistance. Selective genotyping using the iSelect 90K Infinium SNP genotyping array was performed and the resistance locus was mapped to long arm of chromosome 7A and named Yr75. The Axe/Nyb RIL population was genotyped using a targeted genotype-by-sequencing (tGBS) assay and the resistance-linked SNPs were converted into kompetitive allele specific PCR (KASP) markers. These markers were tested on the entire Axe/Nyb RIL population and markers sunKASP_430 and sunKASP_427 showed close association with Yr75 in Axe/Nyabing-3 RIL population. A high-resolution mapping family of 1032 F2 plants from the Axe/Nyb cross was developed and genotyped with sunKASP_430 and sunKASP_427 and these markers flanked Yr75 at 0.3 cM and 0.4 cM, respectively. These markers covers 1.24Mb of the physical map of Chinese Spring and can be used for future map-based cloning of this gene.

scholarly journals Yr58: A New Stripe Rust Resistance Gene and Its Interaction with Yr46 for Enhanced Resistance

2016 ◽  
Vol 106 (12) ◽  
pp. 1530-1534 ◽  
Author(s):  
Mumta Chhetri ◽  
Harbans Bariana ◽  
Pakeerathan Kandiah ◽  
Urmil Bansal
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Infection Type ◽  
Stripe Rust Resistance ◽  
Growth Stages ◽  
Leaf Stage ◽  
Stripe Rust Resistance Gene ◽  
Temperature Regimes ◽  
Ril Population ◽  
Different Growth Stages

The quantitative trait loci QYr.sun-3BS and QYr.sun-4DL were identified in the W195/BTSS recombinant inbred line (RIL) population in a previous study. QYr.sun-3BS explained 34 to 59% phenotypic variation in stripe rust response. We evaluated parental genotypes at different growth stages and temperature regimes to detect the critical stage for expression of QYr.sun-3BS. W195 expressed low infection type (IT) ;1C at the fourth leaf stage, when incubated at 21 ± 2°C and the alternate parent BTSS was susceptible (IT 3+). Monogenic segregation for stripe rust response was observed among the RIL population at the fourth leaf stage and the underlying locus was temporarily named YrW195. YrW195 corresponded to QYr.sun-3BS. Since no previously designated stripe rust resistance genes that expresses at and after the fourth leaf stage was mapped in this region, YrW195 was formally named Yr58. Genotyping with Yr46-linked markers indicated the presence of Yr46 in W195, which corresponded to QYr.sun-4DL. The RILs carrying Yr58 and Yr46 singly produced IT 23C and IT 3+, respectively, and those carrying both genes produced IT ;1C indicating the enhancement of Yr58 expression by Yr46. The absence of Yr58-linked alleles of markers sun533 and sun476 in 74 of the 76 wheat cultivars demonstrated their usefulness for marker-assisted selection.

Genetics of adult plant stripe rust resistance in CSP44, a selection from Australian wheat

2005 ◽  
Vol 84 (3) ◽  
pp. 337-340 ◽  
Author(s):  
Renu Khanna ◽  
U. K. Bansal ◽  
R. G. Saini
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Australian Wheat

scholarly journals Correction to: Genome-wide mapping of adult plant stripe rust resistance in wheat cultivar Toni

2019 ◽  
Vol 132 (7) ◽  
pp. 2167-2168
Author(s):  
Xinli Zhou ◽  
Tian Hu ◽  
Xin Li ◽  
Ma YU ◽  
Yuanyuan Li ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Wheat Cultivar ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Genome Wide

Molecular mapping of quantitative trait loci for adult-plant resistance to powdery mildew in Italian wheat cultivar Libellula

2012 ◽  
Vol 63 (6) ◽  
pp. 539 ◽  
Author(s):  
M. A. Asad ◽  
B. Bai ◽  
C. X. Lan ◽  
J. Yan ◽  
X. C. Xia ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Powdery Mildew ◽  
Stripe Rust ◽  
Plant Resistance ◽  
Quantitative Trait ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Wheat Cultivar ◽  
Stripe Rust Resistance ◽  
Adult Plant

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a fungal disease that causes significant yield losses in many wheat-growing regions of the world. Previously, five quantitative trait loci (QTLs) for adult-plant resistance (APR) to stripe rust resistance were identified in Italian wheat cultivar Libellula. The objectives of this study were to map QTLs for APR to powdery mildew in 244 F2 : 3 lines of Libellula/Huixianhong, to analyse the stability of detected QTLs across environments, and to assess the association of these QTLs with stripe rust resistance. Powdery mildew response was evaluated for 2 years in Beijing and for 1 year in Anyang. The correlation between averaged maximum disease severity (MDS) and averaged area under disease progress curve (AUDPC) over 2 years in Beijing was 0.98, and heritabilities of MDS and AUDPC were 0.65 and 0.81, respectively, based on the mean values averaged across environments. SSR markers were used to screen the parents and mapping population. Five QTLs were identified by inclusive composite interval mapping, designated as QPm.caas-2DS, QPm.caas-4BL.1, QPm.caas-6BL.1, QPm.caas-6BL.2, and QPm.caas-7DS. Three QTLs (QPm.caas-2DS and QPm.caas-6BL.1, and QPm.caas-6BL.2) seem to be new resistance loci for powdery mildew. QTLs QPm.caas-2DS and QPm.caas-4BL.1 were identified at the same position as previously mapped QTLs for stripe rust resistance in Libellula. The QTL QPm.caas-7DS, derived from Libellula, coincided with the slow rusting and slow mildewing locus Lr34/Yr18/Pm38. These results and the identified markers could be useful for wheat breeders aiming for durable resistance to both powdery mildew and stripe rust.

scholarly journals Genome-wide Mapping for Stripe Rust Resistance Loci in Common Wheat Cultivar Qinnong 142

Plant Disease ◽  
2019 ◽  
Vol 103 (3) ◽  
pp. 439-447 ◽  
Author(s):  
Qingdong Zeng ◽  
Jianhui Wu ◽  
Shengjie Liu ◽  
Xianming Chen ◽  
Fengping Yuan ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Wheat Cultivar ◽  
Infection Type ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars

Stripe rust caused by Puccinia striiformis f. sp. tritici threatens worldwide wheat production. Growing resistant cultivars is the best way to control this disease. Chinese wheat cultivar Qinnong 142 (QN142) has a high level of adult-plant resistance to stripe rust. To identify quantitative trait loci (QTLs) related to stripe rust resistance, we developed a recombinant inbred line (RIL) population from a cross between QN142 and susceptible cultivar Avocet S. The parents and 165 F6 RILs were evaluated in terms of their stripe rust infection type and disease severity in replicated field tests with six site-year environments. The parents and RILs were genotyped with single-nucleotide polymorphism (SNP) markers. Four stable QTLs were identified in QN142 and mapped to chromosome arms 1BL, 2AL, 2BL, and 6BS. The 1BL QTL was probably the known resistance gene Yr29, the 2BL QTL was in a resistance gene-rich region, and the 2AL and 6BS QTLs might be new. Kompetitive allele specific polymerase chain reaction markers developed from the SNP markers flanking these QTLs were highly polymorphic in a panel of 150 wheat cultivars and breeding lines. These markers could be used in marker-assisted selection for incorporating the stripe rust resistance QTL into new wheat cultivars.

Genome-wide mapping of adult plant stripe rust resistance in wheat cultivar Toni

2019 ◽  
Vol 132 (6) ◽  
pp. 1693-1704 ◽  
Author(s):  
Xinli Zhou ◽  
Tian Hu ◽  
Xin Li ◽  
Ma YU ◽  
Yuanyuan Li ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Wheat Cultivar ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Genome Wide

scholarly journals Molecular Mapping of Stripe Rust Resistance Gene Yr81 in a Common Wheat Landrace Aus27430

Plant Disease ◽  
2019 ◽  
Vol 103 (6) ◽  
pp. 1166-1171 ◽  
Author(s):  
Mesfin Gessese ◽  
Harbans Bariana ◽  
Debbie Wong ◽  
Matthew Hayden ◽  
Urmil Bansal
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Molecular Mapping ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Chromosomal Assignment ◽  
Resistance Locus ◽  
Sources Of Resistance ◽  
Stripe Rust Resistance Gene ◽  
Ril Population

The deployment of diverse sources of resistance in new cultivars underpins durable control of rust diseases. Aus27430 exhibited a moderate level of stripe rust resistance against Puccinia striiformis f. sp. tritici (Pst) pathotypes currently prevalent in Australia. Aus27430 was crossed with the susceptible parent Avocet S (AvS) and subsequent filial generations were raised. Monogenic segregation observed among Aus27430/AvS F3 families was confirmed through stripe rust screening of an F6 recombinant inbred line (RIL) population, and the resistance locus was temporarily named YrAW5. Selective genotyping using an Illumina iSelect 90K wheat SNP bead chip array located YrAW5 in chromosome 6A. Genetic mapping of the RIL population with linked 90K SNPs that were converted into PCR-based marker assays, as well as SSR markers previously mapped to chromosome 6A, confirmed the chromosomal assignment for YrAW5. Comparative analysis of other stripe rust resistance genes located in chromosome 6A led to the formal designation of YrAW5 as Yr81. Tests with a marker linked with Yr18 also demonstrated the presence of this gene in Aus27430. Yr18 interacted with Yr81 to produce stripe rust responses lower than those produced by RILs carrying these genes individually. Although gwm459 showed higher recombination with Yr81 compared with the other flanking marker KASP_3077, it amplified the AvS allele in 80 cultivars, whereas KASP_3077 amplified AvS allele in 67 cultivars. Both markers can be used in marker-assisted selection after confirming parental polymorphism.

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