Management options for large plants of glyphosate-resistant feather fingergrass (Chloris virgata) in Australian fallow conditions

Chloris virgata has become one of the most difficult glyphosate-resistant (GR) grass weeds in summer fallows in the eastern region of Australia. It germinates in several cohorts following rainfall events; therefore, growers are often tempted to wait for most of the weeds to emerge before herbicide application. However, by that time, some seedlings have reached an advanced stage and there is limited information on the efficacy and reliability of alternate herbicides when targeting large plants of GR C. virgata. A series of experiments were conducted to determine the efficacy of alternate herbicides for the control of GR C. virgata. Haloxyfop (80 g a.i. ha-1) on its own, in mixtures, or sequential applications of haloxyfop and paraquat or glufosinate provided 97 to 100% mortality of the 8–10 leaf stage plants. Glufosinate (1500 g a.i. ha-1) also provided complete control of plants at this growth stage. For larger plants at the 24–28 leaf stage, glufosinate, with or without additional tank-mixed adjuvants, generally did not provide full control, however did show very high levels of biomass reduction and panicle suppression at application rates of 750 or 1500 g a.i. ha-1. Haloxyfop (40 to 160 g a.i. ha-1) and clethodim (180 g a.i. ha-1) on their own achieved 96 to 100% mortality at this growth stage. When applied to large plants (40–50 leaf stage), a tank-mix of isoxaflutole plus paraquat demonstrated significantly higher levels of plant mortality and biomass reduction than either herbicide used alone, and this mixture appears to be synergistic when tested via the Colby equation for synergy or antagonism. Plant mortality was greater (83%) when isoxaflutole (75 g a.i. ha-1) plus paraquat (300 g a.i. ha-1) was taken up through the foliage and soil, compared with the foliage alone. This study identified alternative herbicide options for large plants of GR C. virgata.

The Interrelationship Between Water Use Efficiency and Radiation Use Efficiency Under Progressive Soil Drying in Maize

The maximizing of water use efficiency (WUE) and radiation use efficiency (RUE) is vital to improving crop production in dryland farming systems. However, the fundamental question as to the association of WUE with RUE and its underlying mechanism under limited-water availability remains contentious. Here, a two-year field trial for maize designed with five progressive soil drying regimes applied at two different growth stages (three-leaf stage and seven-leaf stage) was conducted during the 2013–2014 growing seasons. Both environmental variables and maize growth traits at the leaf and canopy levels were measured during the soil drying process. The results showed that leaf WUE increased with irrigation reduction at the early stage, while it decreased with irrigation reduction at the later stage. Leaf RUE thoroughly decreased with irrigation reduction during the progressive soil drying process. Aboveground biomass (AGB), leaf area index (LAI), a fraction of absorbed photosynthetically active radiation (fAPAR), and light extinction coefficient (k) of the maize canopy were significantly decreased by water deficits regardless of the growth stages when soil drying applied. The interrelationships between WUE and RUE were linear across the leaf and canopy scales under different soil drying patterns. Specifically, a positive linear relationship between WUE and RUE are unexpectedly found when soil drying was applied at the three-leaf stage, while it turned out to be negative when soil drying was applied at the seven-leaf stage. Moreover, the interaction between canopy WUE and RUE was more regulated by fAPAR than LAI under soil drying. Our findings suggest that more attention must be paid to fAPAR in evaluating the effect of drought on crops and may bring new insights into the interrelationships of water and radiation use processes in dryland agricultural ecosystems.

Short-Term Waterlogging Depresses Early Growth of Sunflower (Helianthus annuus L.) on Saline Soils with a Shallow Water Table in the Coastal Zone of Bangladesh

Sunflower (Helianthus annuus L.), which is widely grown globally for its high-quality edible oil, is reasonably salt and drought tolerant but it is susceptible to waterlogging. In the saline coastal zone of the Ganges delta, sunflower is often exposed to sudden heavy rainfall during early growth but plant tolerance to such events is not known. Hence, we evaluated the effect of short-term soil inundation (referred to as waterlogging) for 0, 24, 48 and 72 h on sunflower at emergence, 2-leaf, and 4-leaf stages in early- and late-sown crops under field conditions (saline, clay-textured soil, and shallow groundwater). Waterlogging for 24 h did not affect sunflower at any stage but waterlogging for 48 and 72 h suppressed emergence and growth at the 2 and 4-leaf stages. Waterlogging for 72 h completely prevented the emergence for early sowing, whereas emergence was less affected for later sowing. Shoot and root dry weight were most affected at the emergence and 2-leaf stage, not at the 4-leaf stage. In conclusion, waterlogging caused by more than 24 h soil inundation at up to the 4-leaf stage severely depressed emergence and growth, indicating the need for effective drainage at sowing of sunflower in the low-lying coastal saline zone of Bangladesh.

Nitrogen Use Efficiency in Parent vs. Hybrid Canola under Varying Nitrogen Availabilities

Improving nitrogen use efficiency (NUE) is essential for sustainable agriculture, especially in high-N-demanding crops such as canola (Brassica napus). While advancements in above-ground agronomic practices have improved NUE, research on soil and below-ground processes are limited. Plant NUE—and its components, N uptake efficiency (NUpE), and N utilization efficiency (NUtE)—can be further improved by exploring crop variety and soil N cycling. Canola parental genotypes (NAM-0 and NAM-17) and hybrids (H151857 and H151816) were grown on a dark brown chernozem in Saskatchewan, Canada. Soil and plant samples were collected at the 5–6 leaf stage and flowering, and seeds were collected at harvest maturity. Soil N cycling varied with phenotypic stage, with higher potential ammonium oxidation rates at the 5–6 leaf stage and higher urease activity at flowering. Seed N uptake was higher under higher urea-N rates, while the converse was true for NUE metrics. Hybrids had higher yield, seed N uptake, NUtE, and NUE, with higher NUE potentially owing to higher NUtE at flowering, which led to higher yield and seed N allocation. Soil N cycling and soil N concentrations correlated for improved canola NUE, revealing below-ground breeding targets. Future studies should consider multiple root characteristics, including rhizosphere microbial N cycling, root exudates, and root system architecture, to determine the below-ground dynamics of plant NUE.

Evaluation of Complete Fertilizer in the Aspect of the Antioxidant Enzyme System of Maize Hybrids

Studies on physiological and biochemical processes in crops are highly relevant for breeders to produce hybrids with high yield. Two different maturity groups of maize hybrids were tested in this study. The research site was located at the Látókép Experimental Station of the University of Debrecen and the experiment lasted for 2 years. The examined nitrogen ranges were separated into two parts. Firstly, the effects of nitrogen fertilizer ranging from 120–300 kg ha−1 were examined, supplemented with a constant, high-level P2O5 and K2O. Secondly, the optimal ratio of N:P:K was measured. In order to monitor the health status of maize hybrids, stress indicators including the activity of ascorbate peroxidase (APX), and superoxide dismutase (SOD), the rate of lipid peroxidation (LP), and grain yield were measured. The samples were taken in five phenological stages. Variance analysis based on nitrogen fertilizer showed variation in sampling times and fertilizers on APX, LP, and SOD. Variance analysis based on NPK indicated variation in sampling times, years, and fertilizer levels on APX, LP, and SOD. Correlation analysis showed that yield correlated negatively with SOD during the use of NPK fertilizer, as the use of nitrogen fertilizer cannot make corrections to yield with SOD but phosphorus and potassium can correlate with yield, and SOD. Principal component analysis showed that NPK5 and N5 had maximum stability and effect on yield. The activity of APX had the highest value during silking, and LP was in the V14 leaf stage. The correlation and principal component analysis showed that silking and the V14 leaf stage are the most important stages for yield, thus, higher attention must be paid to these stages in the LP and the activation of APX to achieve maximum yield.

Response of Bromus valdivianus (Pasture Brome) Growth and Physiology to Defoliation Frequency Based on Leaf Stage Development

The increase in drought events due to climate change have enhanced the relevance of species with greater tolerance or avoidance traits to water restriction periods, such as Bromus valdivianus Phil. (B. valdivianus). In southern Chile, B. valdivianus and Lolium perenne L. (L. perenne) coexist; however, the pasture defoliation criterion is based on the physiological growth and development of L. perenne. It is hypothesised that B. valdivianus needs a lower defoliation frequency than L. perenne to enhance its regrowth and energy reserves. Defoliation frequencies tested were based on B. valdivianus leaf stage 2 (LS-2), leaf stage 3 (LS-3), leaf stage 4 (LS-4) and leaf stage 5 (LS-5). The leaf stage development of Lolium perenne was monitored and contrasted with that of B. valdivianus. The study was conducted in a glasshouse and used a randomised complete block design. For Bromus valdivianus, the lamina length, photosynthetic rate, stomatal conductance, tiller number per plant, leaf area, leaf weights, root growth rate, water-soluble carbohydrates (WSCs) and starch were evaluated. Bromus valdivianus maintained six live leaves with three leaves growing simultaneously. When an individual tiller started developing its seventh leaf, senescence began for the second leaf (the first relevant leaf for photosynthesis). Plant herbage mass, the root growth rate and tiller growth were maximised at LS-4 onwards. The highest leaf elongation rate, evaluated through the slope of the lamina elongation curve of a fully expanded leaf, was verified at LS-4. The water-soluble carbohydrates (WSCs) increased at LS-5; however, no statistical differences were found in LS-4. The LS-3 and LS-2 treatments showed a detrimental effect on WSCs and regrowth. The leaf photosynthetic rate and stomatal conductance diminished while the leaf age increased. In conclusion, B. valdivianus is a ‘six-leaf’ species with leaf senescence beginning at LS-4.25. Defoliation at LS-4 and LS-5 was optimum for plant regrowth, maximising the aboveground plant parameters and total WSC accumulation. The LS-4 for B. valdivianus was equivalent to LS-3.5 for L. perenne. No differences related to tiller population in B. valdivianus were found in the different defoliation frequencies.

Maize Male Reproductive Organs Affected by Different Timings of Water Deficit

Compared with female reproductive organs, the development of male reproductive organs was got less attention in maize because of its oversupply in amount even under water deficit. Thus, a rainout shelter experiment was designed to explore the effect of different timings of water deficit on pollen vitality and exterior and interior ultra-structure of pollen grains, starch particles in pollen grains, anther fresh weight, and vascular bundle number and its organizational structure in tassel pedicel. There were five water treatments included in this study, viz. well water treatment (CK), water deficit during 6- to 8- leaf stage (V6 − 8), 9- to 12- leaf stage (V9 − 12), 13-leaf stage to tasseling (V13 − T), and silking to blister (R1 − 2), respectively. Results showed that the percentage of pollen grains with strong vitality decreased remarkably by 27.3–45.9% under water deficits, while that of pollen grains with weak vitality increased by 27.2–34.7%. The percentage of pollen grains with no vitality was significantly increased only when water deficit occurred around silking, which was up to 8.6% for V13 − T and 19.7% for R1 − 2 compared with 1.0% for that of CK. Both shrunken pollen apertures (including annulus and operculum) and less starch particles might partially explain the weakened pollen vitality for water deficits before tasseling. Furthermore, the assimilation flux to male reproductive organs might be restricted by the influenced vascular bundle system under water deficits before tasseling, with manifestation showing in anther fresh weight and starch particle status in pollen grains. Specifically, V9 − 12 and V13 − T water deficits delayed differentiation of vascular bundle but had no influence on vascular bundle number, which might be one reason for their decreased anther fresh weight and less starch particles in pollen grains. Conversely, V6 − 8 water deficit significantly decreased vascular bundle number but had no significant influence on anther fresh weight and starch particles in pollen grains. R1 − 2 water deficit almost had no influences on above indicators except for pollen vitality. Overall, this research highlight that male reproductive organs could be influenced by water deficits in maize, which deserves more attention in further breeding especially under the background of high-quality requirement for pollen vitality of the maize hybrids that have a small tassel size.

Rice TSV2 Encoding Threonyl-tRNA Synthetase is Needed for Early Chloroplast Development and Seedling Growth under Cold Stress

Threonyl-tRNA synthetase (ThrRS), one of aminoacyl-tRNA synthetases (AARSs), plays a crucial role in protein synthesis. However, the AARS functions on rice chloroplast development and growth were not fully appraised. In this study, a thermo-sensitive virescent mutant tsv2, which showed albino phenotype and lethal after the 4-leaf stage at 20 °C but recovered to normal when the temperatures rose, was identified and characterized. Map-based cloning and complementation tests showed that TSV2 encoded a chloroplast-located ThrRS protein in rice. The Lys-to-Arg mutation in the anticodon-binding domain hampered chloroplast development under cold stress, while the loss-of-function of the ThrRS core domain in TSV2 fatally led to seedling death regardless of growing temperatures. In addition, TSV2 had a specific expression in early leaves. Its disruption obviously resulted in down-regulation of certain genes associated with chlorophyll biosynthesis, photosynthesis and chloroplast development at cold conditions. Our observations revealed that rice nuclear-encoded TSV2 plays an important role in chloroplast development at the early leaf stage under cold stress.

Quantitative Response of Maize Vcmax25 to Persistent Drought Stress at Different Growth Stages

Drought stress has adverse effects on crop growth and yield, and its identification and monitoring play vital roles in precision crop water management. Accurately evaluating the effect of drought stress on crop photosynthetic capacity can provide a basis for decisions related to crop drought stress identification and monitoring as well as drought stress resistance and avoidance. In this study, the effects of different degrees of persistent drought in different growth stages (3rd leaf stage, 7th leaf stage and jointing stage) on the maximum carboxylation rate at a reference temperature of 25 °C (Vcmax25) of the first fully expanded leaf and its relationship to the leaf water content (LWC) were studied in a field experiment from 2013 to 2015. The results indicated that the LWC decreased continuously as drought stress continued and that the LWC decreased faster in the treatment with more irrigation. Vcmax25 showed a decreasing trend as the drought progressed but had no clear relationship to the growth stage in which the persistent drought occurred. Vcmax25 showed a significantly parabolic relationship (R2 = 0.701, p < 0.001) with the LWC, but the different degrees of persistent drought stress occurring in different growth stages had no distinct effect on the LWC values when Vcmax25 reached its maximum value or zero. The findings of this study also suggested that the LWC was 82.5 ± 0.5% when Vcmax25 reached its maximum value (42.6 ± 3.6 μmol m−2 s−1) and 67.6 ± 1.2% (extreme drought) when Vcmax25 reached zero. These findings will help to improve crop drought management and will be an important reference for crop drought identification, classification and monitoring as well as for the development of drought monitoring and early warning systems for other crops or maize varieties.