Multilocus sequence based systematics of Pseudomonas sp. from supraglacial site of Sikkim Himalaya and their adaptational strategies

Abstract Background Being one of the most complex and diverse genera, deciphering the correct taxonomy of Pseudomonas species has always been challenging. This study investigates and resolves the taxonomic ambiguity of 11 strains of Pseudomonas obtained from the supraglacial site of East Rathong glacier. Since the supraglacial region represents an extreme, stressful environment, the inhabitant microorganisms must have evolved multiple adaptive traits that define their origin. Hence, for adaptation study, we examined the survivability of the 11 strains in physical conditions of freezing and ultraviolet radiation, and their ability to produce extracellular cold-active enzymes. Results Multilocus sequence analysis (MLSA) using five housekeeping genes (1140 polymorphic sites) supported the taxonomic assignment of these strains to Pseudomonas antarctica, further supported by their lesser mean genetic distances with P. antarctica (0.73%) as compared to P. fluorescens (3.65 %). The studied strains displayed significant tolerance to freezing for 96 hours as compared to the mesophilic control strain, while except 4 strains, all strains exhibited substantial tolerance to UV-C radiations, and all strains produced cold active enzymes as well. Conclusion MLSA successfully resolved the taxonomy of these significant group of bacteria from physical extremes of temperature and radiation. The isolates ERGC3:01 and ERGC3:05, owing to their polyadaptational attributes, may be considered promising for exploitation in various industries.

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Cold-active enzymes producing microbes from Gangotri Glacier, western Himalaya

Cryobiology ◽

10.1016/j.cryobiol.2020.10.036 ◽

2020 ◽

Vol 97 ◽

pp. 257-258

Author(s):

Mohammed Kuddus

Keyword(s):

Western Himalaya ◽

Cold Active Enzymes ◽

Cold Active ◽

Gangotri Glacier

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Enzymatic biodegradation of highly p-xylene contaminated soil using cold-active enzymes: A soil column study

Journal of Hazardous Materials ◽

10.1016/j.jhazmat.2021.127099 ◽

2021 ◽

pp. 127099

Author(s):

Saba Miri ◽

Seyyed Mohammadreza Davoodi ◽

Thomas Robert ◽

Satinder Kaur Brar ◽

Richard Martel ◽

...

Keyword(s):

Contaminated Soil ◽

Soil Column ◽

Column Study ◽

Cold Active Enzymes ◽

Cold Active

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Biotechnological potential of psychrophilic microorganisms as the source of cold-active enzymes in food processing applications

3 Biotech ◽

10.1007/s13205-021-03008-y ◽

2021 ◽

Vol 11 (11) ◽

Author(s):

Megha Kumari ◽

Srichandan Padhi ◽

Swati Sharma ◽

Loreni Chiring Phukon ◽

Sudhir P. Singh ◽

...

Keyword(s):

Food Processing ◽

Biotechnological Potential ◽

Cold Active Enzymes ◽

Cold Active

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Bacterial diversity and bioprospecting for cold-active enzymes from culturable bacteria associated with sediment from a melt water stream of Midtre Loenbreen glacier, an Arctic glacier

Research in Microbiology ◽

10.1016/j.resmic.2009.08.008 ◽

2009 ◽

Vol 160 (8) ◽

pp. 538-546 ◽

Cited By ~ 49

Author(s):

Puram Vishnu Vardhan Reddy ◽

Singireesu Soma Shiva Nageswara Rao ◽

Mambatta Shankaranarayanan Pratibha ◽

Buddhi Sailaja ◽

Bakka Kavya ◽

...

Keyword(s):

Bacterial Diversity ◽

Culturable Bacteria ◽

Water Stream ◽

Cold Active Enzymes ◽

Melt Water ◽

Cold Active ◽

Arctic Glacier

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EXTRACELLULAR COLD ACTIVE ENDOGLUCANASE AND PIGMENT PRODUCING PSYCHROTOLERANT PENICILLIUM PINOPHILUM

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i10.13441 ◽

2016 ◽

Vol 8 (10) ◽

pp. 164 ◽

Cited By ~ 1

Author(s):

Abhas Kumar Maharana

Keyword(s):

Sugarcane Bagasse ◽

Endoglucanase Activity ◽

Total Enzyme Activity ◽

Cold Active Enzymes ◽

Red Color ◽

Cold Active ◽

Penicillium Pinophilum ◽

Groundnut Shell ◽

Color Pigment ◽

Total Enzyme

Objective: The objective of the present study was on Penicillium pinophilum strain F2 from soil samples of Jammu city having the potentiality to produce alkaline cold active endoglucanase and pigment.Methods: Penicillium pinophilum strain F2, a psychrotolerant micro-fungus was isolated from soil of Jammu city, India by taking Czapek’s Dox agar incubated at 15 °C. The strain was screened for production of cold active enzymes by taking various substrates at 15 °C. Final production was done for cold active endoglucanase by using sugarcane bagasse and ground nut shell as substrates. Besides, the strain was also able to produce red color pigment at a low temperature which was further studied to optimize its production by changing pH and growth medium. The produced pigment was used for dyeing of wool and silk, and absorption percentages were also calculated.Results: Screening for the production of cold active enzymes revealed it as a good producer of cellulose followed by lipase and amylase. Endoglucanase production revealed the total enzyme titer (total enzyme activity) was found to be 5.032 folds higher in sugarcane bagasse (38.91 units) than groundnut shell (7.732 units). Endoglucanase activity was maximum 9.82±0.33 units/ml and 2.29±0.31 units/ml after 120 h of incubation at 15 °C by sugarcane bagasse and groundnut shells, respectively. Red color pigment production was maxima at pH 5 in Czapek’s Dox broth. Maximum absorption percentage was seen by the treatment soaked with mordant, i.e. 5% CuSO4 (51.52%) and without a mordant, it showed about 45.54%.Conclusion: Due to the above unique features and capability to produce cold active endoglucanase and pigment by strain F2, can be used significantly in various industries.

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Aliivibrio wodanis as a production host: development of genetic tools for expression of cold-active enzymes

Microbial Cell Factories ◽

10.1186/s12934-019-1247-1 ◽

2019 ◽

Vol 18 (1) ◽

Author(s):

Jenny Johansson Söderberg ◽

Miriam Grgic ◽

Erik Hjerde ◽

Peik Haugen

Keyword(s):

Low Temperature ◽

Protein Production ◽

Fluorescent Protein ◽

Active Form ◽

Rhodococcus Erythropolis ◽

Expression Systems ◽

Cold Adapted ◽

Genetic Tools ◽

Cold Active Enzymes ◽

Cold Active

Abstract Background Heterologous production of cold-adapted proteins currently represents one of the greatest bottlenecks in the ongoing bioprospecting efforts to find new enzymes from low-temperature environments, such as, the polar oceans that represent essentially untapped resources in this respect. In mesophilic expression hosts such as Escherichia coli, cold-adapted enzymes often form inactive aggregates. Therefore it is necessary to develop new low-temperature expression systems, including identification of new host organisms and complementary genetic tools. Psychrophilic bacteria, including Pseudoalteromonas haloplanktis, Shewanella and Rhodococcus erythropolis have all been explored as candidates for such applications. However to date none of these have found widespread use as efficient expression systems, or are commercially available. In the present work we explored the use of the sub-Arctic bacterium Aliivibrio wodanis as a potential host for heterologous expression of cold-active enzymes. Results We tested 12 bacterial strains, as well as available vectors, promoters and reporter systems. We used RNA-sequencing to determine the most highly expressed genes and their intrinsic promoters in A. wodanis. In addition we examined a novel 5′-fusion to stimulate protein production and solubility. Finally we tested production of a set of “difficult-to-produce” enzymes originating from various bacteria and one Archaea. Our results show that cold-adapted enzymes can be produced in soluble and active form, even in cases when protein production failed in E. coli due to the formation of inclusion bodies. Moreover, we identified a 60-bp/20-aa fragment from the 5′-end of the AW0309160_00174 gene that stimulates expression of Green Fluorescent Protein and improves production of cold-active enzymes when used as a 5′-fusion. A 25-aa peptide from the same protein enhanced secretion of a 25-aa-sfGFP fusion. Conclusions Our results indicate the use of A. wodanis and associated genetic tools for low-temperature protein production and indicate that A. wodanis represents an interesting platform for further development of a protein production system that can promote further cold-enzyme discoveries.

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Genome Assembly of Chryseobacterium polytrichastri ERMR1:04, a Psychrotolerant Bacterium with Cold Active Proteases, Isolated from East Rathong Glacier in India

Genome Announcements ◽

10.1128/genomea.01305-15 ◽

2015 ◽

Vol 3 (6) ◽

Cited By ~ 8

Author(s):

Rakshak Kumar ◽

Dharam Singh ◽

Mohit Kumar Swarnkar ◽

Anil Kumar Singh ◽

Sanjay Kumar

Keyword(s):

Genome Assembly ◽

Cold Environment ◽

Sikkim Himalaya ◽

Industrial Enzyme ◽

Cold Active ◽

Psychrotolerant Bacterium ◽

Insight Into

We report here the genome assembly of a psychrotolerant bacterium, Chryseobacterium polytrichastri ERMR1:04, which secretes cold-active proteases. The bacterium was isolated from a pristine location, the East Rathong Glacier in the Sikkim Himalaya. The 5.53-Mb genome provides insight into the cold-active industrial enzyme and adaptation in the cold environment.

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