scholarly journals Development of PCR-Based Marker for Resistance to Fusarium wilt Race 2 in Lettuce (Lactuca Sativa L.)

2021 ◽  
Author(s):  
kousuke seki ◽  
kenji komatsu ◽  
masahiro hiraga ◽  
keisuke tanaka ◽  
yuichi uno ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Fine Mapping ◽  
Lactuca Sativa ◽  
Resistance Phenotype ◽  
Resistant Cultivars ◽  
Breeding Technique ◽  
Lactuca Sativa L ◽  
Race 2 ◽  
Selection Of

Abstract Resistance to multiple races of Fusarium wilt is considered a critical trait for lettuce (Lactuca sativa L.) cultivars, as it is directly related to grower profitability in Japan. We analyzed F2 individuals obtained from a cross between ‘VI185’ (resistance to race 2) and ‘ShinanoGreen’ (susceptible to race 2), and applied a bioassay to the F3 population using the race 2 pathogen. ddRAD-seq analysis showed that a single semi-dominant locus on LG1 (qFOL1.2) controls resistance, and that the genotype of a RAD-marker designated as LG1_v8_117.181Mbp showed complete co-segregation with the resistance phenotype based on the F2 population. Fine mapping by PCR-based markers further revealed that qFOL1.2 were located in the position of 116.468-117.974Mbp. The 42 cultivars were evaluated for the genotypes and phenotypes using the PCR-based makers designed at this region. As a result of genotyping, all of the susceptible cultivars had the same genotype in this region, but the resistant cultivars had the diversity in the genotype. Notably, the genotype of the PCR-based marker designated as LG1_v8_116.506Mbp was consistent across the 25 resistant cultivars. Thus, we reasoned that LG1_v8_116.506Mbp was a broadly useful marker for selection of race 2-resistance. Our results provide additional breeding technique for resistance to race 2, and can accelerate pyramiding of resistance loci to multiple races of fusarium wilt.

scholarly journals Variation for Resistance to Verticillium Wilt in Lettuce (Lactuca sativa L.)

Plant Disease ◽  
2007 ◽  
Vol 91 (4) ◽  
pp. 439-445 ◽  
Author(s):  
Ryan J. Hayes ◽  
Gary E. Vallad ◽  
Qing-Ming Qin ◽  
Rebecca C. Grube ◽  
Krishna V. Subbarao
Keyword(s):  
Verticillium Wilt ◽  
Lactuca Sativa ◽  
Field Experiments ◽  
Cost Effective ◽  
Resistant Cultivars ◽  
Lactuca Sativa L ◽  
Cost Effective Method ◽  
Constant Resistance ◽  
Race 1 ◽  
Race 2

Host resistance offers the most cost-effective method of Verticillium wilt control in lettuce (Lactuca sativa). In 2004 and 2005, 107 and 22 lettuce cultivars, respectively, were screened for resistance in a field infested with Verticillium dahliae, and disease progress on resistant and susceptible cultivars was determined. Greenhouse experiments were conducted to evaluate 16 cultivars for resistance to a race 1 and a race 2 isolate. Significant differences for resistance were observed within cultivated lettuce. In susceptible cultivars, disease levels increased through the season, whereas disease in resistant cultivars remained constant. Resistance in greenhouse tests was dependant upon the race used. Seven cultivars were resistant to race 1, whereas all were susceptible to race 2. Cultivar reactions to race 1 in greenhouse and field experiments were correlated, indicating the utility of greenhouse evaluations. The identification of resistance in diverse lettuce types is beneficial to the breeding process. However, because of the existence of resistance-breaking race 2 isolates, this resistance may not be durable. Alternatively, targeted releases of race-1-resistant cultivars to fields with only race 1 pathogen genotypes may extend the life of these cultivars.

scholarly journals Development of PCR-based marker for resistance to Fusarium wilt race 2 in lettuce (Lactuca sativa L.)

Euphytica ◽  
2021 ◽  
Vol 217 (6) ◽  
Author(s):  
Kousuke Seki ◽  
Kenji Komatsu ◽  
Masahiro Hiraga ◽  
Keisuke Tanaka ◽  
Yuichi Uno ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Lactuca Sativa ◽  
Lactuca Sativa L ◽  
Race 2

scholarly journals Analysis of RAPD and AFLP markers linked to resistance to Fusarium oxysporum f. sp. lactucae race 2 in lettuce (Lactuca sativa L.)

Euphytica ◽  
2012 ◽  
Vol 187 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Daisuke Aruga ◽  
Nobuaki Tsuchiya ◽  
Hideo Matsumura ◽  
Etsuo Matsumoto ◽  
Nobuaki Hayashida
Keyword(s):  
Fusarium Oxysporum ◽  
Lactuca Sativa ◽  
Aflp Markers ◽  
Lactuca Sativa L ◽  
Race 2

scholarly journals Introgression of Big Vein Tolerance from Lactuca virosa L. into Cultivated Lettuce (Lactuca sativa L.)

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 881C-881 ◽  
Author(s):  
Ryan J. Hayes* ◽  
Ed Ryder ◽  
Bert Robinson
Keyword(s):  
Great Lakes ◽  
Lactuca Sativa ◽  
Infested Soil ◽  
Hybrid Progeny ◽  
Developmental Processes ◽  
Resistant Cultivars ◽  
Lactuca Virosa ◽  
Lactuca Sativa L ◽  
Congruity Backcrossing ◽  
Linkage Of Genes

Big vein (BV) disease of lettuce is caused by soil borne fungal vectored viruses, and reduces marketability through head deformation. Tolerant cultivars reduce BV frequency, but no resistant cultivars exist. L. virosa L. is highly resistance. The objectives were to 1) determine if L. virosa P.I.s exhibit variation for resistance, and 2) determine if resistance is transferable to lettuce. Seedlings were inoculated with root macerate of BV infected plants, transplanted to BV infested soil, and greenhouse grown for 3 months. Twelve plants in each of 1,2, or 3 reps of Great Lakes 65 (GL65-susceptible), Pavane (Pav-tolerant), L. virosa (11 accessions), and BC1 F2 through F5 families of lettuce cultivars x L. virosa accession IVT280 were tested. The percentage of BV afflicted plants was recorded. In hybrid families, BV free plants from tolerant families were selected and advanced. No BV was found in L. virosa. Variation for tolerance was observed in BC1 F2 and F3 families; 33% had greater tolerance than Pav (17% afflicted). Additional tests identified 11 BC1 F3 families (14%) with greater tolerance than Pav (42% afflicted). Subsequent BC1 F4 and F5 generations however, were more susceptible than Pav. Lactuca virosa is highly resistant, but resistance did not transfer to hybrid progeny. Variation for tolerance was observed in BC1 F2 and F3 families, but later generations were susceptible. Interactions or linkage of genes for developmental processes and BV resistance may hinder introgression. Introgression will continue using congruity backcrossing and a greater diversity of L. virosa.

Identification of two QTLs for resistance to Fusarium wilt race 1 in lettuce (Lactuca sativa L.)

Euphytica ◽  
2020 ◽  
Vol 216 (11) ◽  
Author(s):  
Kousuke Seki ◽  
Kenji Komatsu ◽  
Masahiro Hiraga ◽  
Keisuke Tanaka ◽  
Yuichi Uno ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Lactuca Sativa ◽  
Lactuca Sativa L ◽  
Race 1

Genetics of Partial Resistance Against Verticillium dahliae Race 2 in Wild and Cultivated Lettuce

2020 ◽  
Author(s):  
German Sandoya ◽  
Maria-José Truco ◽  
Lien D Bertier ◽  
Krishna V Subbarao ◽  
Ivan Simko ◽  
...  
Keyword(s):  
Lactuca Sativa ◽  
Verticillium Dahliae ◽  
Partial Resistance ◽  
Dominant Gene ◽  
Genotype By Environment Interaction ◽  
Environment Interaction ◽  
Resistance Qtls ◽  
Lactuca Sativa L ◽  
The Usa ◽  
Race 2

Lettuce (Lactuca sativa L.) is one of the most economically important vegetables in the USA with approximately 50% of the domestic production concentrated in the Salinas Valley of California. Verticillium wilt, caused by races 1 and 2 of the fungal pathogen Verticillium dahliae Kleb., poses a major threat to lettuce production in this area. While resistance governed by a single dominant gene against race 1 has previously been identified and is currently being incorporated into commercial cultivars, identification of resistance against race 2 has been challenging and no lines with complete resistance have been identified. In this study, we screened germplasm for resistance and investigated the genetics of partial resistance against race 2 using three mapping populations derived from crosses involving Lactuca sativa × L. sativa and L. serriola × L. sativa. The inheritance of resistance in Lactuca species against race 2 is complex but a common quantitative trait locus (QTL) on linkage group 6, designated qVERT6.1 (quantitative Verticillium dahliae resistance on LG 6, first QTL), was detected in multiple populations. Additional race 2 resistance QTLs located in several linkage groups were detected in individual populations and environments. Because resistance in lettuce against race 2 is polygenic with a large genotype by environment interaction, breeding programs to incorporate these resistance genes should be aware of this complexity as they implement strategies to control race 2.

scholarly journals Occurrence of Fusarium wilt on Cyclamen Casued by Fusarium oxysporum f. sp. cyclaminis and Selection of Resistant Cultivars

2003 ◽  
Vol 9 (2) ◽  
pp. 79-84
Author(s):  
Jin-Young Kim ◽  
Hong-Gi Kim ◽  
Sun-Sung Hong ◽  
Jin-Won Kim ◽  
Kyeong-Yeol Park
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Resistant Cultivars ◽  
Selection Of

scholarly journals Identification of major quantitative trait loci controlling field resistance to downy mildew in cultivated lettuce (Lactuca sativa L.)

2020 ◽  
Author(s):  
Lorena B Parra ◽  
Ivan Simko ◽  
Richard Michelmore
Keyword(s):  
Downy Mildew ◽  
Lactuca Sativa ◽  
Quantitative Trait ◽  
Durable Resistance ◽  
Gene Pyramiding ◽  
Field Resistance ◽  
Significant Qtls ◽  
Resistant Cultivars ◽  
Lactuca Sativa L ◽  
Grand Rapids

Lettuce downy mildew caused by Bremia lactucae Regel is the most economically important foliar disease of lettuce (Lactuca sativa L.). The deployment of resistant cultivars carrying dominant resistance genes (Dm genes) plays a crucial role in integrated downy mildew disease management; however, high variability in pathogen populations leads to the defeat of plant resistance conferred by Dm genes. Some lettuce cultivars exhibit field resistance that is only manifested in adult plants. Two populations of recombinant inbred lines (RILs), originating from crosses between the field resistant cultivars Grand Rapids and Iceberg and susceptible cultivars Salinas and PI491224, were evaluated for downy mildew resistance under field conditions. One hundred and sixty RILs from the Iceberg × PI491224 and 88 RILs from the Grand Rapids × Salinas RIL populations were genotyped using genotyping by sequencing, which respectively generated 906 and 746 high quality markers that were used for quantitative trait locus (QTL) analysis. We found a QTL in Chromosome 4 that is present in both Grand Rapids × Salinas and Iceberg × PI491224 populations that has a major effect on field resistance. We also found two additional significant QTLs in Chromosomes 2 and 5 in the Iceberg × PI491224 RIL population. Marker-assisted gene pyramiding of multiple Dm genes in combination with QTLs for field resistance provides the opportunity to develop cultivars with more durable resistance to B. lactucae.

Pengaruh Jenis dan Konsentrasi Pupuk Organik Cair terhadap Pertumbuhan dan Produksi Tanaman Selada (Lactuca Sativa l.) secara Hidroponik

2017 ◽  
Vol 2 (01) ◽  
pp. 115-127
Author(s):  
Siti Hilalliyah ◽  
Intan Sari ◽  
Zahlul Ikhsan
Keyword(s):  
Lactuca Sativa ◽  
Lactuca Sativa L

Sistem hidroponik memungkinkan sayuran ditanam di daerah yang kurang subur dan daerah sempit yang padat penduduknya. Alasan penerapan teknik hidroponik yang utama adalah karena terbatasnya lahan pertanian yang produktif untuk memenuhi kebutuhan manusia yang semakin banyak tiap tahunnya, sehingga dibutuhkan suatu terobosan baru untuk memecahkan masalah tersebut. Penelitian ini telah dilaksanakan pada bulan November 2015 sampai bulan Januari 2016 yang bertempat di kampus Fakultas Pertanian Jl. Propinsi Kecamatan Tembilahan Hulu Kabupaten Indragiri Hilir Propinsi Riau.Metode penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) pola faktorial yang terdiri dari 2 faktor. Faktor pertama adalah jenis POC (N) yang terdiri dari 3 taraf perlakuan yaitu POC Jus Bumi, POC Bonggol Pisang danPOC Limbah Sayuran. Faktor kedua adalah konsentrasi larutan (K) yang terdiri dari 3 taraf perlakuan yaitu 150 ppm, 200 ppm dan 250 ppm. Parameter pengamatan adalah Tinggi Tanaman , Jumlah Daun , Luas Daun , Diameter Batang, Panjang akar ,Volume Akar, Kadar Air, dan Bobot Hasil.Hasil pengamatan menunjukkan bahwa perlakuan jenis POC jus bumi dengan konsentrasi larutan 150 ppm memberikan pertumbuhan dan produksi terbaik untuk tanaman selada secara hidroponik sistem wick. Perlakuan jenis POC limbah sayuran dengan konsentrasi 250 ppm memberikan pertumbuhan dan produksi terbaik selain POC jus bumi sehingga dapat dijadikan sumber POC alternatif.

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