scholarly journals Analysis of RAPD and AFLP markers linked to resistance to Fusarium oxysporum f. sp. lactucae race 2 in lettuce (Lactuca sativa L.)

Euphytica ◽  
2012 ◽  
Vol 187 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Daisuke Aruga ◽  
Nobuaki Tsuchiya ◽  
Hideo Matsumura ◽  
Etsuo Matsumoto ◽  
Nobuaki Hayashida
Keyword(s):  
Fusarium Oxysporum ◽  
Lactuca Sativa ◽  
Aflp Markers ◽  
Lactuca Sativa L ◽  
Race 2

scholarly journals Development of PCR-Based Marker for Resistance to Fusarium wilt Race 2 in Lettuce (Lactuca Sativa L.)

2021 ◽  
Author(s):  
kousuke seki ◽  
kenji komatsu ◽  
masahiro hiraga ◽  
keisuke tanaka ◽  
yuichi uno ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Fine Mapping ◽  
Lactuca Sativa ◽  
Resistance Phenotype ◽  
Resistant Cultivars ◽  
Breeding Technique ◽  
Lactuca Sativa L ◽  
Race 2 ◽  
Selection Of

Abstract Resistance to multiple races of Fusarium wilt is considered a critical trait for lettuce (Lactuca sativa L.) cultivars, as it is directly related to grower profitability in Japan. We analyzed F2 individuals obtained from a cross between ‘VI185’ (resistance to race 2) and ‘ShinanoGreen’ (susceptible to race 2), and applied a bioassay to the F3 population using the race 2 pathogen. ddRAD-seq analysis showed that a single semi-dominant locus on LG1 (qFOL1.2) controls resistance, and that the genotype of a RAD-marker designated as LG1_v8_117.181Mbp showed complete co-segregation with the resistance phenotype based on the F2 population. Fine mapping by PCR-based markers further revealed that qFOL1.2 were located in the position of 116.468-117.974Mbp. The 42 cultivars were evaluated for the genotypes and phenotypes using the PCR-based makers designed at this region. As a result of genotyping, all of the susceptible cultivars had the same genotype in this region, but the resistant cultivars had the diversity in the genotype. Notably, the genotype of the PCR-based marker designated as LG1_v8_116.506Mbp was consistent across the 25 resistant cultivars. Thus, we reasoned that LG1_v8_116.506Mbp was a broadly useful marker for selection of race 2-resistance. Our results provide additional breeding technique for resistance to race 2, and can accelerate pyramiding of resistance loci to multiple races of fusarium wilt.

Genetics of Partial Resistance Against Verticillium dahliae Race 2 in Wild and Cultivated Lettuce

2020 ◽  
Author(s):  
German Sandoya ◽  
Maria-José Truco ◽  
Lien D Bertier ◽  
Krishna V Subbarao ◽  
Ivan Simko ◽  
...  
Keyword(s):  
Lactuca Sativa ◽  
Verticillium Dahliae ◽  
Partial Resistance ◽  
Dominant Gene ◽  
Genotype By Environment Interaction ◽  
Environment Interaction ◽  
Resistance Qtls ◽  
Lactuca Sativa L ◽  
The Usa ◽  
Race 2

Lettuce (Lactuca sativa L.) is one of the most economically important vegetables in the USA with approximately 50% of the domestic production concentrated in the Salinas Valley of California. Verticillium wilt, caused by races 1 and 2 of the fungal pathogen Verticillium dahliae Kleb., poses a major threat to lettuce production in this area. While resistance governed by a single dominant gene against race 1 has previously been identified and is currently being incorporated into commercial cultivars, identification of resistance against race 2 has been challenging and no lines with complete resistance have been identified. In this study, we screened germplasm for resistance and investigated the genetics of partial resistance against race 2 using three mapping populations derived from crosses involving Lactuca sativa × L. sativa and L. serriola × L. sativa. The inheritance of resistance in Lactuca species against race 2 is complex but a common quantitative trait locus (QTL) on linkage group 6, designated qVERT6.1 (quantitative Verticillium dahliae resistance on LG 6, first QTL), was detected in multiple populations. Additional race 2 resistance QTLs located in several linkage groups were detected in individual populations and environments. Because resistance in lettuce against race 2 is polygenic with a large genotype by environment interaction, breeding programs to incorporate these resistance genes should be aware of this complexity as they implement strategies to control race 2.

scholarly journals Variation for Resistance to Verticillium Wilt in Lettuce (Lactuca sativa L.)

Plant Disease ◽  
2007 ◽  
Vol 91 (4) ◽  
pp. 439-445 ◽  
Author(s):  
Ryan J. Hayes ◽  
Gary E. Vallad ◽  
Qing-Ming Qin ◽  
Rebecca C. Grube ◽  
Krishna V. Subbarao
Keyword(s):  
Verticillium Wilt ◽  
Lactuca Sativa ◽  
Field Experiments ◽  
Cost Effective ◽  
Resistant Cultivars ◽  
Lactuca Sativa L ◽  
Cost Effective Method ◽  
Constant Resistance ◽  
Race 1 ◽  
Race 2

Host resistance offers the most cost-effective method of Verticillium wilt control in lettuce (Lactuca sativa). In 2004 and 2005, 107 and 22 lettuce cultivars, respectively, were screened for resistance in a field infested with Verticillium dahliae, and disease progress on resistant and susceptible cultivars was determined. Greenhouse experiments were conducted to evaluate 16 cultivars for resistance to a race 1 and a race 2 isolate. Significant differences for resistance were observed within cultivated lettuce. In susceptible cultivars, disease levels increased through the season, whereas disease in resistant cultivars remained constant. Resistance in greenhouse tests was dependant upon the race used. Seven cultivars were resistant to race 1, whereas all were susceptible to race 2. Cultivar reactions to race 1 in greenhouse and field experiments were correlated, indicating the utility of greenhouse evaluations. The identification of resistance in diverse lettuce types is beneficial to the breeding process. However, because of the existence of resistance-breaking race 2 isolates, this resistance may not be durable. Alternatively, targeted releases of race-1-resistant cultivars to fields with only race 1 pathogen genotypes may extend the life of these cultivars.

scholarly journals Development of PCR-based marker for resistance to Fusarium wilt race 2 in lettuce (Lactuca sativa L.)

Euphytica ◽  
2021 ◽  
Vol 217 (6) ◽  
Author(s):  
Kousuke Seki ◽  
Kenji Komatsu ◽  
Masahiro Hiraga ◽  
Keisuke Tanaka ◽  
Yuichi Uno ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Lactuca Sativa ◽  
Lactuca Sativa L ◽  
Race 2

scholarly journals Controle de Rhizoctonia solani e Fusarium oxysporum f.sp. phaseoli por biopreparados de isolados de Trichoderma spp.

2013 ◽  
Vol 39 (4) ◽  
pp. 258-262 ◽  
Author(s):  
Pedro Paulo Dias ◽  
Ricardo Luis Louro Berbara ◽  
Maria do Carmo de Araújo Fernandes
Keyword(s):  
Phaseolus Vulgaris ◽  
Rhizoctonia Solani ◽  
Fusarium Oxysporum ◽  
Lactuca Sativa ◽  
Damping Off ◽  
Phaseolus Vulgaris L ◽  
Trichoderma Spp ◽  
Lactuca Sativa L

Os experimentos objetivaram avaliar em condições de casa de vegetação o biocontrole dos fitopatógenos Rhizoctonia solani (RS) e Fusarium oxysporum f.sp. phaseoli (FOP) em alface (Lactuca sativa L.) cultivar Regina, e feijão-vagem (Phaseolus vulgaris L.) cultivar Alessa, respectivamente, utilizando como agentes antagonistas, 10 isolados de Trichoderma spp. selecionados em testes in vitro. Foram feitos biopreparados à base de arroz previamente colonizado por isolados de Trichoderma spp. e posteriormente triturados. Para a realização dos testes, os biopreparados foram inoculados previamente na proporção de 10(9) conídios.mL-1, em substrato comercial para produção de mudas. Após sete dias, os patógenos foram introduzidos separadamente em duas concentrações distintas: R. solani na proporção de 144 mg de meio de arroz por kg de substrato e F. oxysporum f.sp. phaseoli inoculado na forma de suspensão contendo 4,75 x 10(6) conídios.mL-1. Avaliou-se a influência dos biopreparados na % de damping-off de pós-emergência em plantas de alface e a severidade de murcha em plantas de feijão-vagem. O biopreparado referente ao isolado T-03 foi o mais eficiente no controle de R. solani em plantas de alface cultivar Regina, por ter reduzido a incidência de damping-off de pós-emergência nessa cultura. Por outro lado, nenhum dos biopreparados apresentou efeito antagonista satisfatório à F. oxysporum f.sp. phaseoli em plantas de feijão-vagem.

Pengaruh Jenis dan Konsentrasi Pupuk Organik Cair terhadap Pertumbuhan dan Produksi Tanaman Selada (Lactuca Sativa l.) secara Hidroponik

2017 ◽  
Vol 2 (01) ◽  
pp. 115-127
Author(s):  
Siti Hilalliyah ◽  
Intan Sari ◽  
Zahlul Ikhsan
Keyword(s):  
Lactuca Sativa ◽  
Lactuca Sativa L

Sistem hidroponik memungkinkan sayuran ditanam di daerah yang kurang subur dan daerah sempit yang padat penduduknya. Alasan penerapan teknik hidroponik yang utama adalah karena terbatasnya lahan pertanian yang produktif untuk memenuhi kebutuhan manusia yang semakin banyak tiap tahunnya, sehingga dibutuhkan suatu terobosan baru untuk memecahkan masalah tersebut. Penelitian ini telah dilaksanakan pada bulan November 2015 sampai bulan Januari 2016 yang bertempat di kampus Fakultas Pertanian Jl. Propinsi Kecamatan Tembilahan Hulu Kabupaten Indragiri Hilir Propinsi Riau.Metode penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) pola faktorial yang terdiri dari 2 faktor. Faktor pertama adalah jenis POC (N) yang terdiri dari 3 taraf perlakuan yaitu POC Jus Bumi, POC Bonggol Pisang danPOC Limbah Sayuran. Faktor kedua adalah konsentrasi larutan (K) yang terdiri dari 3 taraf perlakuan yaitu 150 ppm, 200 ppm dan 250 ppm. Parameter pengamatan adalah Tinggi Tanaman , Jumlah Daun , Luas Daun , Diameter Batang, Panjang akar ,Volume Akar, Kadar Air, dan Bobot Hasil.Hasil pengamatan menunjukkan bahwa perlakuan jenis POC jus bumi dengan konsentrasi larutan 150 ppm memberikan pertumbuhan dan produksi terbaik untuk tanaman selada secara hidroponik sistem wick. Perlakuan jenis POC limbah sayuran dengan konsentrasi 250 ppm memberikan pertumbuhan dan produksi terbaik selain POC jus bumi sehingga dapat dijadikan sumber POC alternatif.

KHẢO SÁT DƯ LƯỢNG NITRAT VÀ KIM LOẠI NẶNG (Cu, Pb, Zn) TRONG CÂY RAU XÀ LÁCH (Lactuca sativa L.) TRỒNG Ở PHƯỜNG HƯƠNG LONG - THÀNH PHỐ HUẾ

2015 ◽  
Vol 100 (1) ◽  
Author(s):  
Nguyễn Minh Trí ◽  
Nguyễn Hạnh Trinh ◽  
Nguyễn Thị Hoàng Phương
Keyword(s):  
Lactuca Sativa ◽  
Viet Nam ◽  
Lactuca Sativa L ◽  
Tu 45

Xà lách (Lactuca sativa L.) là một loại rau ăn lá quan trọng có giá trị dinh dưỡng và kinh tế cao. Cây Xà lách có đặc điểm là loại rau ngắn ngày, có thời gian sinh trưởng ngắn khoảng từ 45 - 55 ngày, có thể phát triển tốt trên nhiều loại đất, là loại rau ăn sống được sử dụng rất phổ biến trong bữa ăn hàng ngày của người dân Việt Nam nên nó được trồng quanh năm, do vậy vấn đề về chất lượng lại càng phải được quan tâm nhiều hơn. Bài báo này giới thiệu kết quả phân tích về dư lượng nitrat và các kim loại nặng (Cu, Pb, Zn) trong rau Xà lách vụ Xuân - Hè 2012 - 2013 ở phường Hương Long - thành phố Huế. Kết quả nghiên cứu cho thấy: đất trồng rau Xà lách tại phường Hương Long – thành phố Huế đạt tiêu chuẩn về hàm lượng kim loại nặng (Pb, Zn) theo QCVN 03:2008/BTNMT, nhưng hàm lượng Cu và nitrat là khá cao. Rau Xà lách thành phẩm có dư lượng nitrat cao hơn 1,21% so với quy định và các kim loại nặng (Pb, Zn, Cu) tồn dư trong rau lại ở mức cao và vượt tiêu chuẩn cho phép nhiều lần sẽ ảnh hưởng đến sức khỏe của người sử dụng.

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