scholarly journals Non-contact, in-vivo, functional, and structural ophthalmic imaging using multimodal photoacoustic remote sensing (PARS) microscopy and swept source optical coherence tomography (SS-OCT)

2021 ◽  
Author(s):  
Zohreh Hosseinaee ◽  
Nima Abbasi ◽  
Layla Khalili ◽  
Lyazzat Mukhangaliyeva ◽  
Nicholas Pellegrino ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Optical Coherence Tomography ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Ocular Tissue ◽  
Optical Coherence ◽  
Structural Imaging ◽  
Ocular Diseases ◽  
Swept Source

Abstract Early diagnosis of ocular diseases improves the understanding of pathophysiology and aids in accurate monitoring and effective treatment. Advanced, multimodal ocular imaging platforms play a crucial role in visualization of ocular components and provide clinicians with a valuable tool for evaluating various eye diseases. Here, for the first time we present a non-contact, multiwavelength photoacoustic remote sensing (PARS) microscopy and swept-source optical coherence tomography (SS-OCT) for in-vivo functional and structural imaging of the eye. The system provides complementary imaging contrasts of optical absorption and optical scattering, and is used for non-contact, in-vivo imaging of murine eye. Results of vasculature and structural imaging as well as melanin content in the retinal pigment epithelium layer are presented. Multiwavelength PARS microscopy using Stimulated Raman Scattering is applied to enable in-vivo, non-contact oxygen saturation estimation in the ocular tissue. The reported work may be a major step towards clinical translation of ophthalmic technologies and has the potential to advance the diagnosis and treatment of ocular diseases.

scholarly journals Functional and structural ophthalmic imaging using noncontact multimodal photoacoustic remote sensing microscopy and optical coherence tomography

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zohreh Hosseinaee ◽  
Nima Abbasi ◽  
Nicholas Pellegrino ◽  
Layla Khalili ◽  
Lyazzat Mukhangaliyeva ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Optical Coherence Tomography ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Ocular Tissue ◽  
Optical Coherence ◽  
Structural Imaging ◽  
Ocular Diseases ◽  
Major Step

AbstractEarly diagnosis of ocular diseases improves the understanding of pathophysiology and aids in accurate monitoring and effective treatment. Advanced, multimodal ocular imaging platforms play a crucial role in visualization of ocular components and provide clinicians with a valuable tool for evaluating various eye diseases. Here, for the first time we present a non-contact, multiwavelength photoacoustic remote sensing (PARS) microscopy and swept-source optical coherence tomography (SS-OCT) for in-vivo functional and structural imaging of the eye. The system provides complementary imaging contrasts of optical absorption and optical scattering, and is used for simultaneous, non-contact, in-vivo imaging of murine eye. Results of vasculature and structural imaging as well as melanin content in the retinal pigment epithelium layer are presented. Multiwavelength PARS microscopy using Stimulated Raman scattering is applied to enable in-vivo, non-contact oxygen saturation estimation in the ocular tissue. The reported work may be a major step towards clinical translation of ophthalmic technologies and has the potential to advance the diagnosis and treatment of ocular diseases.

scholarly journals In vivo optical biopsy of choroidal osteoma: a swept source optical coherence tomography–based tumor characterization

2020 ◽  
Vol 12 ◽  
pp. 251584142092274 ◽  
Author(s):  
Shorya Vardhan Azad ◽  
Vinod Kumar ◽  
Rohan Chawla ◽  
Bibhuti Kashyap ◽  
Shreyas Temkar ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Signal Transmission ◽  
Retinal Pigment ◽  
Optical Coherence ◽  
Choroidal Neovascular Membrane ◽  
Swept Source ◽  
Retinal Layers ◽  
Choroidal Osteoma

Objective: To study tumor characteristics of choroidal osteoma by swept source optical coherence tomography. Methods: A retrospective case series done at tertiary referral center in northern India. All patients diagnosed with choroidal osteoma examined on swept source optical coherence tomography were included. Swept source optical coherence tomography images were analyzed for integrity of retinal layers—intraretinal layers, outer retinal layers (photoreceptor), retinal pigment epithelium, and contour abnormalities. Choroidal changes assessed were tumor attributes such as shape, depth of choroidal involvement, tumor mass reflectivity pattern, tumor vascularity, and evolutionary structural abnormalities such as deossification, focal depressions, or choroidal neovascular membrane. Results: A total of 15 eyes of 11 patients were analyzed. Seven of 11 patients were females. Mean age of presentation was approximately 26 years. Tumor was large in nine cases (>7.5 mm). Deossification was seen in 12 eyes. Inner and outer retinal integrity was maintained in 7 and 2 eyes, respectively. Most common internal tumor reflectivity pattern seen was a lamellar appearance (12/15). Increased signal transmission to choroid and focal area of deep excavation was present in 11 and 4 eyes, respectively. Osteoclastic activity was noted in 12 eyes. Choroidal neovascular membrane was seen in 6 eyes. Small lesions showed lamellar pattern of tumor reflectivity with preservation of retinal pigment epithelium and overlying retina. Larger tumors were deossified with 6 irregular tumor contour, disorganization of the outer retina, increased signal transmission to choroid, and areas of osteoclastic activity. Conclusion: Swept source optical coherence tomography was helpful in assessing tumor attributes and predicting the different timelines in tumor evolution.

scholarly journals Hyperspectral optical coherence tomography for in vivo visualization of melanin in the retinal pigment epithelium

2019 ◽  
Vol 12 (12) ◽  
Author(s):  
Danielle J. Harper ◽  
Thomas Konegger ◽  
Marco Augustin ◽  
Kornelia Schützenberger ◽  
Pablo Eugui ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Optical Coherence

In vivo depth structural imaging of rat olfactory bulb with a swept source optical coherence tomography

2011 ◽  
Vol 71 ◽  
pp. e207
Author(s):  
Hideyuki Watanabe ◽  
Uma Maheswari Rajagopalan ◽  
Yu Nakamichi ◽  
Kei Igarashi ◽  
Violeta Dimitrova Madjarova ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Olfactory Bulb ◽  
Optical Coherence ◽  
Structural Imaging ◽  
Swept Source

scholarly journals In vivo Optical Coherence Tomography of Light-Driven Melanosome Translocation in Retinal Pigment Epithelium

2013 ◽  
Vol 3 (1) ◽  
Author(s):  
Qiu-Xiang Zhang ◽  
Rong-Wen Lu ◽  
Jeffrey D. Messinger ◽  
Christine A. Curcio ◽  
Vincenzo Guarcello ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Optical Coherence

Acute macular neuroretinopathy: pathogenetic insights from optical coherence tomography angiography

2018 ◽  
Vol 103 (3) ◽  
pp. 410-414 ◽  
Author(s):  
Giuseppe Casalino ◽  
Alessandro Arrigo ◽  
Francesco Romano ◽  
Marion R Munk ◽  
Francesco Bandello ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence Tomography Angiography ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Vessel Density ◽  
Control Group ◽  
Optical Coherence ◽  
Cross Sectional ◽  
Swept Source ◽  
Acute Macular Neuroretinopathy

AimTo describe the optical coherence tomography angiography (OCT-A) features of patients affected by acute macular neuroretinopathy (AMN).Methods This is a prospective, observational, cross-sectional study. Multimodal imaging, including spectral domain OCT (Spectralis HRA+OCT; Heidelberg Engineering, Heidelberg, Germany) and 6×6 mm swept source OCT-A (ZEISS PLEX Elite 9000; ZEISS, Dublin, California), was performed on all patients. The primary outcome measure was assessment of the vascular alterations of the superficial (SCP) and deep (DCP) capillary plexuses and choriocapillaris (CC). A segmentation of the AMN lesions using enface OCT images of photoreceptors-retinal pigment epithelium complex was used to discriminate intralesional and extralesional regions on the OCT-A enface slabs of the DCP and CC reconstructions. Each OCT-A slab was imported into ImageJ V.1.50 and digitally binarised for quantitative analyses.ResultsOverall, seven patients (mean age 19.4±3.2 years, six women) affected by AMN were included. The mean best-corrected visual acuity was 0.00±0.00 logarithm of the minimum angle of resolution. Twelve healthy age-matched subjects (mean age 22±3.4 years, 10 women) represented the control group. The quantitative analysis of global vessel densities showed that the CC vessel density was significantly lower in patients with AMN (0.495±0.03) compared with the age-matched controls (0.545±0.02) (corrected p=0.0003). The intralesional vessel density of the DCP was 0.349±0.04 in AMN and vs 0.497±0.02 in the controls (corrected p=0.0002).ConclusionsOur study confirmed inner choroidal vascular flow void as a possible pathogenetic mechanism of AMN. We also found a focal impairment of the DCP within the AMN lesions. Future studies are needed to clarify which is the primary location of the vascular insult in this condition.

scholarly journals Choroidal Imaging with Swept Source Optical Coherence Tomography – A Review

2014 ◽  
Vol 08 (02) ◽  
pp. 132 ◽  
Author(s):  
Katarzyna Piasecka ◽  
Zofia Michalewska ◽  
◽  
◽  
Keyword(s):  
Optical Coherence Tomography ◽  
Vascular Tissue ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Tunable Laser ◽  
Laser Source ◽  
Optical Coherence ◽  
Swept Source ◽  
Spectral Domain Oct ◽  
And Function

The choroid provides up to 70 % of blood and oxygen to the eye. Pathological changes of this vascular tissue may lead to malnutrition of the retina and therefore be involved in the pathogenesis of numerous retinal disorders. Swept source optical coherence tomography (SS-OCT) is a new development of a non-invasive imaging technique that uses a tunable laser source with a higher wavelength light than conventional spectral domain OCT (SD-OCT). This enables visualisation of tissues below the retinal pigment epithelium. Thanks to SS-OCT’s ultrahigh speed and ultrahigh resolution it is possible to assess choroidal layers as well as to automatically create 3D maps of its thickness and volume. This review is to discuss how SS-OCT has improved our understanding of choroidal anatomy and function in various retinal and chorioretinal diseases. In future, detailed evaluation of choroid may play a crucial role in the diagnosis and management of various retinal diseases.

Microstructural Changes in Central Serous Chorioretinopathy Documented by Spectral-domain Optical Coherence Tomography

2013 ◽  
Vol 07 (02) ◽  
pp. 98 ◽  
Author(s):  
Dominik Odrobina ◽  
Piotr Gozdek ◽  
Mariusz Maroszyński ◽  
Iwona Laudańska-Olszewska ◽  
◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Central Serous Chorioretinopathy ◽  
Pigment Epithelium ◽  
Rapid Development ◽  
Retinal Pigment ◽  
Active Phase ◽  
Spectral Domain ◽  
Optical Coherence ◽  
Microstructural Changes

Central serous chorioretinopathy (CSC) was first described more than 140 years ago. Due to the rapid development in modern imaging methods, better understanding of changes occurring in the retina in CSC is possible. Spectral-domain optical coherence tomography (SDOCT) has increased our ability to study this disease, especially microstructural changes during active phase and after resolution of CSC. SD-OCT enables a highly detailedin vivoevaluation of the individual retinal layers especially external limiting membrane (ELM), the inner and outer segments of photoreceptors and changes in retinal pigment epithelium (RPE), which are the most essential and important in described disease. It allows us better understand pathogenesis of CSC.

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