Heterologous Expression of Arabidopsis thaliana rty Gene in Strawberry (Fragaria × ananassa Duch.) Improves Drought Tolerance

Abstract Background: Strawberry (Fragaria × ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutant rty of Arabidopsis thaliana ROOTY (RTY) results in increased endogenous auxin levels, more roots, and shoot growth. It is still unclear whether the rty gene improves stress tolerance in strawberry. Results: rty gene was isolated from Arabidopsis thaliana and placed under the control of the cauliflower mosaic virus (CaMV) 35S promoter in the pBI121-rty binary vector carrying the selectable marker of neomycin phosphotransferase Ⅱ (NPT Ⅱ). Seven transgenic lines were confirmed by PCR and western blot analysis. Accumulations of IAA and ABA were significantly increased in the transgenic plants. The endogenous IAA contents were 46.5 ng g–1 and 66.0 ng g–1in control and transgenic plants respectively. The endogenous ABA contents in the control plant were 236.3 ng g–1 and in transgenic plants were 543.8 ng g–1. The production of adventitious roots and trichomes were enhanced in the transgenic plants. Furthermore, transcript levels of the genes including IAA and ABA biosynthetic, and stress-responsive genes, were higher in the transgenic plants than in the control plants under drought conditions. Water use efficiency and a reduced water loss rate were enhanced in the transgenic strawberry plants. Additionally, peroxidase and catalase activities were significantly higher in the transgenic plants than in the control plants. The experiment results revealed a novel function for rty related to ABA and drought responses. Conclusions: The rty gene improved hormone-mediated drought tolerance in transgenic strawberry. The heterologous expression of rty in strawberry improved drought tolerance by promoting auxin and ABA accumulation. These phytohormones together brought about various physiological changes that improved drought tolerance via increased root production, trichome density, and stomatal closure. Our results suggested that a transgenic approach can be used to overcome the inherent trade-off between plant growth and drought tolerance by enhancing water use efficiency and reducing water loss rate under water shortage conditions.

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Heterologous Expression of Arabidopsis thaliana rty Gene in Strawberry (Fragaria × ananassa Duch.) Improves Drought Tolerance

10.21203/rs.3.rs-34565/v2 ◽

2020 ◽

Author(s):

Maofu Li ◽

Yuan Yang ◽

Ali Raza ◽

Shanshan Yin ◽

Hua Wang ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Transgenic Plants ◽

Drought Tolerance ◽

Heterologous Expression ◽

Water Use Efficiency ◽

Water Use ◽

Water Loss ◽

Loss Rate ◽

Water Loss Rate ◽

Use Efficiency

Abstract Background: Strawberry (Fragaria × ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutant rty of Arabidopsis thaliana ROOTY (RTY) results in increased endogenous auxin levels, more roots, and shoot growth. It is still unclear whether the rty gene improves stress tolerance in strawberry. Results: rty gene was isolated from Arabidopsis thaliana and placed under the control of the cauliflower mosaic virus (CaMV) 35S promoter in the pBI121-rty binary vector carrying the selectable marker of neomycin phosphotransferaseⅡ(NPTⅡ). Seven transgenic lines were confirmed by PCR and western blot analysis. Accumulations of IAA and ABA were significantly increased in the transgenic plants. The endogenous IAA contents were 46.5 ng g–1 and 66.0 ng g–1in control and transgenic plants respectively. The endogenous ABA contents in the control plant were 236.3 ng g–1 and in transgenic plants were 543.8 ng g–1. The production of adventitious roots and trichomes were enhanced in the transgenic plants. Furthermore, transcript levels of the genes including IAA and ABA biosynthetic, and stress-responsive genes, were higher in the transgenic plants than in the control plants under drought conditions. Water use efficiency and a reduced water loss rate were enhanced in the transgenic strawberry plants. Additionally, peroxidase and catalase activities were significantly higher in the transgenic plants than in the control plants. The experiment results revealed a novel function for rty related to ABA and drought responses. Conclusions: The rty gene improved hormone-mediated drought tolerance in transgenic strawberry. The heterologous expression of rty in strawberry improved drought tolerance by promoting auxin and ABA accumulation. These phytohormones together brought about various physiological changes that improved drought tolerance via increased root production, trichome density, and stomatal closure. Our results suggested that a transgenic approach can be used to overcome the inherent trade-off between plant growth and drought tolerance by enhancing water use efficiency and reducing water loss rate under water shortage conditions.

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Heterologous expression of Arabidopsis thaliana rty gene in strawberry (Fragaria × ananassa Duch.) improves drought tolerance

BMC Plant Biology ◽

10.1186/s12870-021-02839-4 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Maofu Li ◽

Yuan Yang ◽

Ali Raza ◽

Shanshan Yin ◽

Hua Wang ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Transgenic Plants ◽

Drought Tolerance ◽

Heterologous Expression ◽

Water Use Efficiency ◽

Water Use ◽

Water Loss ◽

Loss Rate ◽

Water Loss Rate ◽

Use Efficiency

Abstract Background Strawberry (Fragaria × ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutant rty of Arabidopsis thaliana ROOTY (RTY) results in increased endogenous auxin levels, more roots, and shoot growth. It is still unclear whether the rty gene improves stress tolerance in strawberry. Results rty gene was isolated from Arabidopsis thaliana and placed under the control of the cauliflower mosaic virus (CaMV) 35S promoter in the pBI121-rty binary vector carrying the selectable marker of neomycin phosphotransferase II (NPT II). Seven transgenic lines were confirmed by PCR and western blot analysis. Accumulations of IAA and ABA were significantly increased in the transgenic plants. The endogenous IAA contents were 46.5 ng g− 1 and 66.0 ng g− 1in control and transgenic plants respectively. The endogenous ABA contents in the control plant were 236.3 ng g− 1 and in transgenic plants were 543.8 ng g− 1. The production of adventitious roots and trichomes were enhanced in the transgenic plants. Furthermore, transcript levels of the genes including IAA and ABA biosynthetic, and stress-responsive genes, were higher in the transgenic plants than in the control plants under drought conditions. Water use efficiency and a reduced water loss rate were enhanced in the transgenic strawberry plants. Additionally, peroxidase and catalase activities were significantly higher in the transgenic plants than in the control plants. The experiment results revealed a novel function for rty related to ABA and drought responses. Conclusions The rty gene improved hormone-mediated drought tolerance in transgenic strawberry. The heterologous expression of rty in strawberry improved drought tolerance by promoting auxin and ABA accumulation. These phytohormones together brought about various physiological changes that improved drought tolerance via increased root production, trichome density, and stomatal closure. Our results suggested that a transgenic approach can be used to overcome the inherent trade-off between plant growth and drought tolerance by enhancing water use efficiency and reducing water loss rate under water shortage conditions.

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Heterologous Expression of Arabidopsis rty Enhances Drought Tolerance in Strawberry (Fragaria × ananassa Duch.)

10.21203/rs.3.rs-34565/v1 ◽

2020 ◽

Cited By ~ 3

Author(s):

Maofu Li ◽

Yuan Yang ◽

Ali Raza ◽

Shanshan Yin ◽

Hua Wang ◽

...

Keyword(s):

Transgenic Plants ◽

Drought Tolerance ◽

Heterologous Expression ◽

Water Use ◽

Water Loss ◽

Loss Rate ◽

Stomatal Closure ◽

Fragaria Ananassa ◽

Water Loss Rate ◽

Use Efficiency

Abstract Background Strawberry ( Fragaria × ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutation of Arabidopsis thaliana ROOTY ( RTY ) results in increased endogenous auxin levels and roots and shoot growth, but the effects of this gene in strawberry remain unclear. Results Here, we heterologously expressed Arabidopsis rty in strawberry plants and examined the effects of rty expression on the hormonal and physiological properties of the plants. Heterologous expression of rty induced IAA accumulation and increased the production of adventitious roots as well as trichomes on the abaxial leaf surface of the transgenic plants. Furthermore, the transgenic strawberry plants had increased ABA accumulation and stomatal closure. The transgenic strawberry plants exhibited enhanced water use efficiency and a reduced water loss rate. Additionally, peroxidase and catalase activities were significantly higher in the transgenic plants than in the untransformed controls, and the transgenic plants were more drought tolerant than the wild-type plants. Our results uncover a transgenic approaches can be used to overcome the inherent trade-off between plant growth and drought tolerance by enhancing water use efficiency and reducing water loss rate under water shortage conditions. Conclusions In this study, the rty gene improves hormone-mediated drought tolerance in transgenic strawberry. We demonstrated that the heterologous expression of rty in strawberry improved drought tolerance by promoting auxin and ABA accumulation. These phytohormones together brought about various physiological changes that improved drought tolerance via increased root production, trichome density, and stomatal closure. This study provides the basis for future genetic modifications of strawberry to improve drought tolerance.

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Intrinsic water use efficiency at the pollination stage as a parameter for drought tolerance selection in Phaseolus vulgaris

Physiologia Plantarum ◽

10.1034/j.1399-3054.1999.106206.x ◽

1999 ◽

Vol 106 (2) ◽

pp. 184-189 ◽

Cited By ~ 37

Author(s):

C. Pimentel ◽

D. Laffray ◽

P. Louguet

Keyword(s):

Phaseolus Vulgaris ◽

Drought Tolerance ◽

Water Use Efficiency ◽

Water Use ◽

Intrinsic Water Use Efficiency ◽

Use Efficiency

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The physiological basis for genetic variation in water use efficiency and carbon isotope composition in Arabidopsis thaliana

Photosynthesis Research ◽

10.1007/s11120-013-9891-5 ◽

2013 ◽

Vol 119 (1-2) ◽

pp. 119-129 ◽

Cited By ~ 49

Author(s):

Hsien Ming Easlon ◽

Krishna S. Nemali ◽

James H. Richards ◽

David T. Hanson ◽

Thomas E. Juenger ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Genetic Variation ◽

Water Use Efficiency ◽

Carbon Isotope ◽

Water Use ◽

Isotope Composition ◽

Carbon Isotope Composition ◽

Physiological Basis ◽

Use Efficiency

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Characterization of wheat genotypes for drought tolerance and water use efficiency

Scientia Agricola ◽

10.1590/1678-992x-2019-0304 ◽

2021 ◽

Vol 78 (5) ◽

Author(s):

Guilherme Filgueiras Soares ◽

Walter Quadros Ribeiro Júnior ◽

Lucas Felisberto Pereira ◽

Cristiane Andréa de Lima ◽

Daiane dos Santos Soares ◽

...

Keyword(s):

Drought Tolerance ◽

Water Use Efficiency ◽

Water Use ◽

Wheat Genotypes ◽

Use Efficiency

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Overexpression of an aquaporin gene PvPIP2;9 improved biomass yield, protein content, drought tolerance and water use efficiency in switchgrass ( Panicum virgatum L.)

GCB Bioenergy ◽

10.1111/gcbb.12751 ◽

2020 ◽

Vol 12 (11) ◽

pp. 979-991

Author(s):

Jing Zhang ◽

Wuwu Wen ◽

Hui Li ◽

Qiyu Lu ◽

Bin Xu ◽

...

Keyword(s):

Drought Tolerance ◽

Water Use Efficiency ◽

Protein Content ◽

Water Use ◽

Panicum Virgatum ◽

Biomass Yield ◽

Panicum Virgatum L ◽

Aquaporin Gene ◽

Use Efficiency

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Transcription co-activatorArabidopsisANGUSTIFOLIA3 (AN3) regulates water-use efficiency and drought tolerance by modulating stomatal density and improving root architecture by the transrepression ofYODA(YDA)

Plant Biotechnology Journal ◽

10.1111/pbi.12324 ◽

2015 ◽

Vol 13 (7) ◽

pp. 893-902 ◽

Cited By ~ 35

Author(s):

Lai-Sheng Meng ◽

Shun-Qiao Yao

Keyword(s):

Drought Tolerance ◽

Water Use Efficiency ◽

Water Use ◽

Root Architecture ◽

Stomatal Density ◽

Use Efficiency

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Effects of Drought Frequency on Growth Performance and Transpiration of Young Black Locust (Robinia pseudoacaciaL.)

International Journal of Forestry Research ◽

10.1155/2014/821891 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 12

Author(s):

Dario Mantovani ◽

Maik Veste ◽

Dirk Freese

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Water Use Efficiency ◽

Growth Performance ◽

Water Use ◽

Water Availability ◽

Black Locust ◽

Marginal Land ◽

Drought Tolerant ◽

Use Efficiency

Black locust (Robinia pseudoacaciaL.) is a drought-tolerant fast growing tree, which could be an alternative to the more common tree species used in short-rotation coppice on marginal land. The plasticity of black locust in the form of ecophysiological and morphological adaptations to drought is an important precondition for its successful growth in such areas. However, adaptation to drought stress is detrimental to primary production. Furthermore, the soil water availability condition of the initial stage of development may have an impact on the tree resilience. We aimed to investigate the effect of drought stress applied during the resprouting on the drought tolerance of the plant, by examining the black locust growth patterns. We exposed young trees in lysimeters to different cycles of drought. The drought memory affected the plant growth performance and its drought tolerance: the plants resprouting under drought conditions were more drought tolerant than the well-watered ones. Black locust tolerates drastic soil water availability variations without altering its water use efficiency (2.57 g L−1), evaluated under drought stress. Due to its constant water use efficiency and the high phenotypic plasticity, black locust could become an important species to be cultivated on marginal land.

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