Modelling water use efficiency in a dynamic environment: An example using Arabidopsis thaliana

Abstract Background: Strawberry (Fragaria × ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutant rty of Arabidopsis thaliana ROOTY (RTY) results in increased endogenous auxin levels, more roots, and shoot growth. It is still unclear whether the rty gene improves stress tolerance in strawberry. Results: rty gene was isolated from Arabidopsis thaliana and placed under the control of the cauliflower mosaic virus (CaMV) 35S promoter in the pBI121-rty binary vector carrying the selectable marker of neomycin phosphotransferaseⅡ(NPTⅡ). Seven transgenic lines were confirmed by PCR and western blot analysis. Accumulations of IAA and ABA were significantly increased in the transgenic plants. The endogenous IAA contents were 46.5 ng g–1 and 66.0 ng g–1in control and transgenic plants respectively. The endogenous ABA contents in the control plant were 236.3 ng g–1 and in transgenic plants were 543.8 ng g–1. The production of adventitious roots and trichomes were enhanced in the transgenic plants. Furthermore, transcript levels of the genes including IAA and ABA biosynthetic, and stress-responsive genes, were higher in the transgenic plants than in the control plants under drought conditions. Water use efficiency and a reduced water loss rate were enhanced in the transgenic strawberry plants. Additionally, peroxidase and catalase activities were significantly higher in the transgenic plants than in the control plants. The experiment results revealed a novel function for rty related to ABA and drought responses. Conclusions: The rty gene improved hormone-mediated drought tolerance in transgenic strawberry. The heterologous expression of rty in strawberry improved drought tolerance by promoting auxin and ABA accumulation. These phytohormones together brought about various physiological changes that improved drought tolerance via increased root production, trichome density, and stomatal closure. Our results suggested that a transgenic approach can be used to overcome the inherent trade-off between plant growth and drought tolerance by enhancing water use efficiency and reducing water loss rate under water shortage conditions.

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Direct and indirect selection on flowering time, water‐use efficiency ( WUE , δ 13 C), and WUE plasticity to drought in Arabidopsis thaliana

Ecology and Evolution ◽

10.1002/ece3.1270 ◽

2014 ◽

Vol 4 (23) ◽

pp. 4505-4521 ◽

Cited By ~ 65

Author(s):

Amanda M. Kenney ◽

John K. McKay ◽

James H. Richards ◽

Thomas E. Juenger

Keyword(s):

Arabidopsis Thaliana ◽

Water Use Efficiency ◽

Flowering Time ◽

Water Use ◽

Indirect Selection ◽

Use Efficiency

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Determination of Water Use Efficiency for Arabidopsis thaliana

BIO-PROTOCOL ◽

10.21769/bioprotoc.1041 ◽

2014 ◽

Vol 4 (3) ◽

Cited By ~ 4

Author(s):

Weronika Wituszynska ◽

Stanisław Karpiński

Keyword(s):

Arabidopsis Thaliana ◽

Water Use Efficiency ◽

Water Use ◽

Use Efficiency

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Heterologous Expression of Arabidopsis thaliana rty Gene in Strawberry (Fragaria × ananassa Duch.) Improves Drought Tolerance

10.21203/rs.3.rs-34565/v3 ◽

2021 ◽

Author(s):

Maofu Li ◽

Yuan Yang ◽

Ali Raza ◽

Shanshan Yin ◽

Hua Wang ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Transgenic Plants ◽

Drought Tolerance ◽

Heterologous Expression ◽

Water Use Efficiency ◽

Water Use ◽

Water Loss ◽

Loss Rate ◽

Water Loss Rate ◽

Use Efficiency

Abstract Background: Strawberry (Fragaria × ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutant rty of Arabidopsis thaliana ROOTY (RTY) results in increased endogenous auxin levels, more roots, and shoot growth. It is still unclear whether the rty gene improves stress tolerance in strawberry. Results: rty gene was isolated from Arabidopsis thaliana and placed under the control of the cauliflower mosaic virus (CaMV) 35S promoter in the pBI121-rty binary vector carrying the selectable marker of neomycin phosphotransferase Ⅱ (NPT Ⅱ). Seven transgenic lines were confirmed by PCR and western blot analysis. Accumulations of IAA and ABA were significantly increased in the transgenic plants. The endogenous IAA contents were 46.5 ng g–1 and 66.0 ng g–1in control and transgenic plants respectively. The endogenous ABA contents in the control plant were 236.3 ng g–1 and in transgenic plants were 543.8 ng g–1. The production of adventitious roots and trichomes were enhanced in the transgenic plants. Furthermore, transcript levels of the genes including IAA and ABA biosynthetic, and stress-responsive genes, were higher in the transgenic plants than in the control plants under drought conditions. Water use efficiency and a reduced water loss rate were enhanced in the transgenic strawberry plants. Additionally, peroxidase and catalase activities were significantly higher in the transgenic plants than in the control plants. The experiment results revealed a novel function for rty related to ABA and drought responses. Conclusions: The rty gene improved hormone-mediated drought tolerance in transgenic strawberry. The heterologous expression of rty in strawberry improved drought tolerance by promoting auxin and ABA accumulation. These phytohormones together brought about various physiological changes that improved drought tolerance via increased root production, trichome density, and stomatal closure. Our results suggested that a transgenic approach can be used to overcome the inherent trade-off between plant growth and drought tolerance by enhancing water use efficiency and reducing water loss rate under water shortage conditions.

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Natural variation in stomata size contributes to the local adaptation of water-use efficiency in Arabidopsis thaliana

10.1101/253021 ◽

2018 ◽

Cited By ~ 2

Author(s):

H. Dittberner ◽

A. Korte ◽

T. Mettler-Altmann ◽

A.P.M. Weber ◽

G. Monroe ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Genetic Variation ◽

Water Use Efficiency ◽

Water Use ◽

Natural Variation ◽

Regional Differentiation ◽

Carbon Uptake ◽

Trade Offs ◽

Stomata Size ◽

Use Efficiency

AbstractStomata control gas exchanges between the plant and the atmosphere. How natural variation in stomata size and density contributes to resolve trade-offs between carbon uptake and water-loss in response to local climatic variation is not yet understood. We developed an automated confocal microscopy approach to characterize natural genetic variation in stomatal patterning in 330 fully-sequenced Arabidopsis thaliana accessions collected throughout the European range of the species. We compared this to variation in water-use efficiency, measured as carbon isotope discrimination (δ13C). We detect substantial genetic variation for stomata size and density segregating within Arabidopsis thaliana. A positive correlation between stomata size and δ13C further suggests that this variation has consequences on water-use efficiency. Genome-wide association analyses indicate a complex genetic architecture underlying not only variation in stomata patterning but also to its co-variation with carbon uptake parameters. Yet, we report two novel QTL affecting δ13C independently of stomata patterning. This suggests that, in A. thaliana, both morphological and physiological variants contribute to genetic variance in water-use efficiency. Patterns of regional differentiation and co-variation with climatic parameters indicate that natural selection has contributed to shape some of this variation, especially in Southern Sweden, where water availability is more limited in spring relative to summer. These conditions are expected to favor the evolution of drought avoidance mechanisms over drought escape strategies.

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Characterization of transgenic cotton (Gossypium hirsutum L.) over-expressing Arabidopsis thaliana Related to ABA-insensitive3(ABI3)/Vivparous1 (AtRAV1) and AtABI5 transcription factors: improved water use efficiency through altered guard cell physiology

Plant Biotechnology Reports ◽

10.1007/s11816-017-0455-6 ◽

2017 ◽

Vol 11 (5) ◽

pp. 339-353 ◽

Cited By ~ 1

Author(s):

Justin G. Fiene ◽

Sayani Mallick ◽

Amandeep Mittal ◽

Christian Nansen ◽

Lauren Kalns ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Transcription Factors ◽

Water Use Efficiency ◽

Water Use ◽

Guard Cell ◽

Transgenic Cotton ◽

Cell Physiology ◽

Gossypium Hirsutum L ◽

Use Efficiency

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Heterologous expression of Arabidopsis thaliana rty gene in strawberry (Fragaria × ananassa Duch.) improves drought tolerance

BMC Plant Biology ◽

10.1186/s12870-021-02839-4 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Maofu Li ◽

Yuan Yang ◽

Ali Raza ◽

Shanshan Yin ◽

Hua Wang ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Transgenic Plants ◽

Drought Tolerance ◽

Heterologous Expression ◽

Water Use Efficiency ◽

Water Use ◽

Water Loss ◽

Loss Rate ◽

Water Loss Rate ◽

Use Efficiency

Abstract Background Strawberry (Fragaria × ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutant rty of Arabidopsis thaliana ROOTY (RTY) results in increased endogenous auxin levels, more roots, and shoot growth. It is still unclear whether the rty gene improves stress tolerance in strawberry. Results rty gene was isolated from Arabidopsis thaliana and placed under the control of the cauliflower mosaic virus (CaMV) 35S promoter in the pBI121-rty binary vector carrying the selectable marker of neomycin phosphotransferase II (NPT II). Seven transgenic lines were confirmed by PCR and western blot analysis. Accumulations of IAA and ABA were significantly increased in the transgenic plants. The endogenous IAA contents were 46.5 ng g− 1 and 66.0 ng g− 1in control and transgenic plants respectively. The endogenous ABA contents in the control plant were 236.3 ng g− 1 and in transgenic plants were 543.8 ng g− 1. The production of adventitious roots and trichomes were enhanced in the transgenic plants. Furthermore, transcript levels of the genes including IAA and ABA biosynthetic, and stress-responsive genes, were higher in the transgenic plants than in the control plants under drought conditions. Water use efficiency and a reduced water loss rate were enhanced in the transgenic strawberry plants. Additionally, peroxidase and catalase activities were significantly higher in the transgenic plants than in the control plants. The experiment results revealed a novel function for rty related to ABA and drought responses. Conclusions The rty gene improved hormone-mediated drought tolerance in transgenic strawberry. The heterologous expression of rty in strawberry improved drought tolerance by promoting auxin and ABA accumulation. These phytohormones together brought about various physiological changes that improved drought tolerance via increased root production, trichome density, and stomatal closure. Our results suggested that a transgenic approach can be used to overcome the inherent trade-off between plant growth and drought tolerance by enhancing water use efficiency and reducing water loss rate under water shortage conditions.

Download Full-text