scholarly journals Rhabdonatronobacter Sediminivivens gen. nov., sp. nov. Isolated from the Sediment of Hutong Qagan Soda Lake

2021 ◽  
Author(s):  
Heng Zhou ◽  
Ming Yang ◽  
Qiong Xue ◽  
Shengjie Zhang ◽  
Jian Zhou ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
New Genus ◽  
Gene Sequence ◽  
Novel Species ◽  
Soda Lake ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
The Family ◽  
Inner Mongolia Autonomous Region ◽  
Genomic Relatedness

Abstract A novel Gram-stain negative bacterium, designated IM2376T, was isolated from the sediment of Hutong Qagan Lake in Ordos, Inner Mongolia Autonomous Region of China. The strain IM2376T had the highest similarity with Roseinatronobacter thiooxidans DSM 13087T (96.18%) and Rhodobaca bogoriensis LBB1T (96.18%) of the family Rhodobacteraceae according to 16S rRNA gene sequence comparison. Genomic relatedness analyses showed that strain IM2376T was clearly distinguished from other species in the family Rhodobacteraceae, with average nucleotide identities, amino acid identities and in silico DNA-DNA hybridization values not more than 74.1%, 68.5% and 20.2%. The fatty acid was mainly composed of C18:1ω7c (64.86%), iso-C16:0 (16.33%) and C16: 1ω7c/C16:1ω6c (6.02%). The major polar lipid was diphosphatidyl glycerol, phosphatidylglycerol and phosphatidylcholine. The predominant ubiquinone was Q-10 (94.9%) and Q-11 (5.1%). The DNA G + C was 66 mol%. Based on all these results, strain IM2376T was considered to be a novel species of a new genus in the family Rhodobacteraceae, for which the name Rhabdonatronobacter sediminivivens gen. nov., sp. nov. is proposed. The type strain is IM2376T (= CGMCC 1.17852T).

scholarly journals Halolamina pelagica gen. nov., sp. nov., a new member of the family Halobacteriaceae

2011 ◽  
Vol 61 (7) ◽  
pp. 1617-1621 ◽  
Author(s):  
Heng-Lin Cui ◽  
Xia Gao ◽  
Xin Yang ◽  
Xue-Wei Xu
Keyword(s):  
Dna Hybridization ◽  
New Genus ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Marine Solar Saltern ◽  
The Family ◽  
Nacl Concentration ◽  
Optimum Ph

Two extremely halophilic archaeal strains, TBN21T and TBN49, were isolated from the Taibei marine solar saltern near Lianyungang city, Jiangsu province, China. Cells of the two strains were pleomorphic and Gram-negative and colonies were red. Strains TBN21T and TBN49 were able to grow at 25–50 °C (optimum 37 °C), at 1.4–5.1 M NaCl (optimum 3.4–3.9 M) and at pH 5.5–9.5 (optimum pH 7.0–7.5) and neither strain required Mg2+ for growth. Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). The major polar lipids of the two strains were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and eight glycolipids; three of these glycolipids (GL3, GL4 and GL5) were chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1), galactosyl mannosyl glucosyl diether (TGD-1) and mannosyl glucosyl diether (DGD-1), respectively. Phylogenetic analysis revealed that strains TBN21T and TBN49 formed a distinct clade with their closest relative, Halobaculum gomorrense JCM 9908T (89.0–89.5 % 16S rRNA gene sequence similarity). The DNA G+C contents of strains TBN21T and TBN49 were 64.8 and 62.7 mol%, respectively. DNA–DNA hybridization between strains TBN21T and TBN49 was 90.1 %. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains TBN21T and TBN49 represent a novel species in a new genus within the family Halobacteriaceae, for which the name Halolamina pelagica gen. nov., sp. nov. is proposed. The type strain of Halolamina pelagica is TBN21T ( = CGMCC 1.10329T  = JCM 16809T).

Ornithinibacter aureus gen. nov., sp. nov., a novel member of the family Intrasporangiaceae

2011 ◽  
Vol 61 (3) ◽  
pp. 659-664 ◽  
Author(s):  
Chuan Xiao ◽  
Huiqin Huang ◽  
Jianjun Ye ◽  
Xiaopeng Wu ◽  
Jun Zhu ◽  
...  
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Gram Stain ◽  
Rrna Gene Sequence ◽  
Cellular Fatty Acids ◽  
The Family ◽  
Diamino Acid

A novel strain of the class Actinobacteria was isolated from a seawater sample collected in the South China Sea using modified R2A agar plates. The strain was a Gram-stain-positive, non-motile, non-spore-forming, catalase-positive, irregular rod-shaped bacterium. The strain grew at 4–45 °C and pH 5.0–10.2, and tolerated 5 % (w/v) NaCl. Based on its 16S rRNA gene sequence, the organism was related phylogenetically to members of the genera Fodinibacter (96.7 % similarity), Lapillicoccus (96.5 %), Knoellia (95.0–95.8 %), Oryzihumus (95.6 %) and Humibacillus (95.6 %). The cell-wall contained l-ornithine as the major diagnostic diamino acid in the peptidoglycan. MK-8(H4) was the predominant menaquinone. Major cellular fatty acids were iso-C18 : 1 ω9c, iso-C16 : 0, iso-C15 : 0 and C17 : 0. The G+C content of the DNA was 69.6 mol%. Phenotypic and phylogenetic data revealed that this strain represents a novel species in a new genus of the family Intrasporangiaceae, for which the name Ornithinibacter aureus gen. nov., sp. nov. is proposed; the type strain of Ornithinibacter aureus is HB09001T (=CGMCC 1.10341T =DSM 23364T).

scholarly journals Luteipulveratus halotolerans sp. nov., an actinobacterium (Dermacoccaceae) from forest soil

2015 ◽  
Vol 65 (Pt_11) ◽  
pp. 4113-4120 ◽  
Author(s):  
Holed Juboi ◽  
Ann Anni Basik ◽  
Sunita Sara Gill Shamsul ◽  
Phil Arnold ◽  
Esther K. Schmitt ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Monophyletic Clade ◽  
Phenotypic Characteristics ◽  
Pacbio Rs Ii ◽  
The Family ◽  
The 16S Rrna Gene

The taxonomic position of an actinobacterium strain, C296001T, isolated from a soil sample collected in Sarawak, Malaysia, was established using a polyphasic approach. Phylogenetically, strain C296001T was closely associated with the genus Luteipulveratus and formed a distinct monophyletic clade with the only described species, Luteipulveratus mongoliensis NBRC 105296T. The 16S rRNA gene sequence similarity between strain C296001T and L. mongoliensis was 98.7 %. DNA–DNA hybridization results showed that the relatedness of strain C296001T to L. mongoliensis was only 21.5 %. The DNA G+C content of strain C296001T was 71.7 mol%. Using a PacBio RS II system, whole genome sequences for strains C296001T and NBRC 105296T were obtained. The genome sizes of 4.5 Mbp and 5.4 Mbp determined were similar to those of other members of the family Dermacoccaceae. The cell-wall peptidoglycan contained lysine, alanine, aspartic acid, glutamic acid and serine, representing the peptidoglycan type A4α l-Lys-l-Ser-d-Asp. The major menaquinones were MK-8(H4), MK-8 and MK-8(H2). Phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol and phosphoglycolipid were the polar lipids, while the whole-cell sugars were glucose, fucose and lesser amounts of ribose and galactose. The major fatty acids were iso-C16 : 0, anteiso-C17 : 0, iso-C16 : 1 H, anteiso-C17 : 1ω9c, iso-C18 : 0 and 10-methyl C17 : 0. Chemotaxonomic analyses showed that C296001T had typical characteristics of members of the genus Luteipulveratus, with the main differences occurring in phenotypic characteristics. On the basis of the phenotypic and chemotaxonomic evidence, it is proposed that strain C296001T be classified as a representative of a novel species in the genus Luteipulveratus, for which the name Luteipulveratus halotolerans sp. nov. is recommended. The type strain is C296001T ( = ATCC TSD-4T = JCM 30660T).

scholarly journals Herminiimonas arsenicoxydans sp. nov., a metalloresistant bacterium

2006 ◽  
Vol 56 (8) ◽  
pp. 1765-1769 ◽  
Author(s):  
Daniel Muller ◽  
Diliana D. Simeonova ◽  
Philippe Riegel ◽  
Sophie Mangenot ◽  
Sandrine Koechler ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Carbon Sources ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Industrial Sludge ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Curved Rods

An arsenite-oxidizing bacterium, designated strain ULPAs1T, was isolated from industrial sludge heavily contaminated with arsenic. Cells of this isolate were Gram-negative, curved rods, motile by means of a polar flagellum. The strain was positive for oxidase and catalase activities, was able to reduce nitrate to nitrite, used acetate, lactate and peptone as organic carbon sources under aerobic conditions and was able to oxidize arsenite (As[III]) to arsenate (As[V]). 16S rRNA gene sequence analysis and the absence of dodecanoic fatty acids suggested that this strain represents a member of the genus Herminiimonas of the family Oxalobacteraceae, order Burkholderiales in the Betaproteobacteria. Genomic DNA–DNA hybridization between strain ULPAs1T and Herminiimonas fonticola S-94T and between strain ULPAs1T and Herminiimonas aquatilis CCUG 36956T revealed levels of relatedness of <10 %, well below the recommended 70 % species cut-off value. Thus, strain ULPAs1T (=CCM 7303T=DSM 17148T=LMG 22961T) is the type strain of a novel species of Herminiimonas, for which the name Herminiimonas arsenicoxydans sp. nov. is proposed.

Amnibacterium kyonggiense gen. nov., sp. nov., a new member of the family Microbacteriaceae

2011 ◽  
Vol 61 (1) ◽  
pp. 155-159 ◽  
Author(s):  
Sun-Jung Kim ◽  
Sang-Seob Lee
Keyword(s):  
New Genus ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Diamino Acid ◽  
Taxonomic Approach

A Gram-positive, non-motile bacterium, designated KSL51201-037T, was isolated from Anyang stream, Republic of Korea, and was characterized using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis showed that strain KSL51201-037T belonged to the family Microbacteriaceae of the class Actinobacteria and exhibited 96.9 % gene sequence similarity to Labedella gwakjiensis KSW2-17T, 96.0 % to Leifsonia ginsengi wged11T and 95.9 % to Microterricola viridarii KV-677T. The G+C content of the genomic DNA was 72.7 mol%. Strain KSL51201-037T had l-2,4-diaminobutyric acid as the diagnostic cell-wall diamino acid, MK-11 and MK-12 as the major menaquinones, anteiso-C15 : 0 (47.8 %) and iso-C16 : 0 (24.0 %) as the major fatty acids and phosphatidylglycerol and three unknown phospholipids as the major polar lipids. On the basis of phenotypic and genotypic properties and phylogenetic distinctiveness, it is suggested that strain KSL51201-037T represents a novel species of a new genus in the family Microbacteriaceae for which the name Amnibacterium kyonggiense gen. nov., sp. nov. is proposed. The type strain of the type species is KSL51201-037T (=KEMC 51201-037T=JCM 16463T).

scholarly journals Salsuginibacillus halophilus sp. nov., a halophilic bacterium isolated from a soda lake

2010 ◽  
Vol 60 (6) ◽  
pp. 1339-1343 ◽  
Author(s):  
Shu-Juan Cao ◽  
Jian-Hang Qu ◽  
Hong-Li Yuan ◽  
Bao-Zhen Li
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Soda Lake ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Optimum Ph ◽  
Unknown Structure ◽  
Inner Mongolia Autonomous Region

A Gram-stain-positive, rod-shaped, endospore-forming, halophilic, alkalitolerant bacterium, designated halo-1T, was isolated from sediment of Xiarinaoer soda lake, located in the Inner Mongolia Autonomous Region of China. Strain halo-1T grew in the presence of 9–30 % (w/v) NaCl (optimum 19 %) and at pH 5–10 (optimum pH 9). The cell-wall peptidoglycan contained meso-diaminopimelic acid and the major respiratory isoprenoid quinone was MK-7. The predominant cellular fatty acids of the isolate were anteiso-C15 : 0 (58.35 %), anteiso-C17 : 0 (12.89 %) and C16 : 0 (6.52 %). The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, glycolipid and a phospholipid of unknown structure. The DNA G+C content of the strain was 46.4 mol%. On the basis of 16S rRNA gene sequence similarity, strain halo-1T showed the highest similarity (93.9 %) to Salsuginibacillus kocurii CH9dT. Strain halo-1T could be clearly differentiated from its closest phylogenetic relative on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain halo-1T represents a novel species, for which the name Salsuginibacillus halophilus sp. nov. is proposed, with the type strain halo-1T (=CGMCC 1.7653T =NBRC 104934T).

scholarly journals Zobellia amurskyensis sp. nov., Zobellia laminariae sp. nov. and Zobellia russellii sp. nov., novel marine bacteria of the family Flavobacteriaceae

2004 ◽  
Vol 54 (5) ◽  
pp. 1643-1648 ◽  
Author(s):  
Olga I. Nedashkovskaya ◽  
Makoto Suzuki ◽  
Marc Vancanneyt ◽  
Ilse Cleenwerck ◽  
Anatoly M. Lysenko ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Gram Negative ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Type Strains

The taxonomic position of four newly isolated marine, heterotrophic, gliding, Gram-negative, aerobic, pigmented, agarolytic bacteria was established. 16S rRNA gene sequence analysis indicated affiliation of the isolates to the genus Zobellia in the family Flavobacteriaceae. DNA–DNA hybridization experiments revealed that the strains studied represent three distinct and novel species, for which the names Zobellia amurskyensis sp. nov., Zobellia laminariae sp. nov. and Zobellia russellii sp. nov. are proposed, with KMM 3526T (=LMG 22069T=CCUG 47080T), KMM 3676T (=LMG 22070T=CCUG 47083T) and KMM 3677T (=LMG 22071T=CCUG 47084T), respectively, as the type strains.

scholarly journals Formosa algae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae

2004 ◽  
Vol 54 (3) ◽  
pp. 705-711 ◽  
Author(s):  
Elena P. Ivanova ◽  
Yulia V. Alexeeva ◽  
Sébastien Flavier ◽  
Jonathan P. Wright ◽  
Natalia V. Zhukova ◽  
...  
Keyword(s):  
New Genus ◽  
Gene Sequence ◽  
Novel Species ◽  
Enrichment Culture ◽  
Rrna Gene ◽  
16S Rdna Sequence ◽  
Light Yellow ◽  
The Family ◽  
Phyletic Line ◽  
Sequence Similarities

Four light-yellow-pigmented, Gram-negative, short-rod-shaped, non-motile isolates were obtained from enrichment culture during degradation of the thallus of the brown alga Fucus evanescens. The isolates studied were chemo-organotrophic, alkalitolerant and mesophilic. Polar lipids were analysed and phosphatidylethanolamine was the only phospholipid identified. The predominant cellular fatty acids were 15 : 0, i15 : 0, ai15 : 0, i15 : 1 and 15 : 1(n-6). The DNA G+C contents of the four strains were 34·0–34·4 mol%. The level of DNA relatedness of the four isolates was conspecific (88–98 %), indicating that they belong to the same species. The 16S rDNA sequence of strain KMM 3553T was determined. Phylogenetic analysis revealed that KMM 3553T formed a distinct phyletic line in the phylum Bacteroidetes, class Flavobacteria in the family Flavobacteriaceae and that, phylogenetically, this strain could be placed almost equidistant from the genera Gelidibacter and Psychroserpens (16S rRNA gene sequence similarities of 94 %). On the basis of significant differences in phenotypic and chemotaxonomic characteristics, it is suggested that the isolates represent a novel species in a new genus; the name Formosa algae gen. nov., sp. nov. is proposed. The type strain is KMM 3553T (=CIP 107684T).

scholarly journals Bacillus lehensis sp. nov., an alkalitolerant bacterium isolated from soil

2007 ◽  
Vol 57 (2) ◽  
pp. 238-242 ◽  
Author(s):  
A. Ghosh ◽  
M. Bhardwaj ◽  
T. Satyanarayana ◽  
M. Khurana ◽  
S. Mayilraj ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Phenotypic Characteristics ◽  
Phenotypic Properties ◽  
Low Levels ◽  
Type Strains ◽  
Genomic Relatedness

A Gram-positive, endospore-forming, alkalitolerant bacterial strain, designated MLB2T, was isolated from soil from Leh, India, and was subjected to a polyphasic taxonomic study. The strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus Bacillus. Growth was observed at pH 7.0–11.0, but not at pH 6.0. The DNA G+C content was 41.4 mol%. The highest level of 16S rRNA gene sequence similarity was with Bacillus oshimensis JCM 12663T (98.8 %). However, DNA–DNA hybridization experiments indicated low levels of genomic relatedness with the type strains of B. oshimensis (62 %), Bacillus patagoniensis (55 %), Bacillus clausii (51 %) and Bacillus gibsonii (34 %), the species with which strain MLB2T formed a coherent cluster (based on the results of the phylogenetic analysis). On the basis of the phenotypic characteristics and genotypic distinctiveness of strain MLB2T, it should be classified within a novel species of Bacillus, for which the name Bacillus lehensis sp. nov. is proposed. The type strain is MLB2T (=MTCC 7633T=JCM 13820T).

scholarly journals Investigation of taxa of the family Pasteurellaceae isolated from Syrian and European hamsters and proposal of Mesocricetibacter intestinalis gen. nov., sp. nov. and Cricetibacter osteomyelitidis gen. nov., sp. nov.

2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3636-3643 ◽  
Author(s):  
H. Christensen ◽  
W. Nicklas ◽  
M. Bisgaard
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
New Genus ◽  
Gene Sequence ◽  
Published Data ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Type Strains

Eleven strains from hamster of Bisgaard taxa 23 and 24, also referred to as Krause’s groups 2 and 1, respectively, were investigated by a polyphasic approach including data published previously. Strains showed small, regular and circular colonies with smooth and shiny appearance, typical of members of the family Pasteurellaceae . The strains formed two monophyletic groups based on 16S rRNA gene sequence comparison to other members of the family Pasteurellaceae . Partial rpoB sequencing as well as published data on DNA–DNA hybridization showed high genotypic relationships within both groups. Menaquinone 7 (MK7) was found in strains of both groups as well as an unknown ubiquinone with shorter chain length than previously reported for any other member of the family Pasteurellaceae . A new genus with one species, Mesocricetibacter intestinalis gen. nov., sp. nov., is proposed to accommodate members of taxon 24 of Bisgaard whereas members of taxon 23 of Bisgaard are proposed to represent Cricetibacter osteomyelitidis gen. nov., sp. nov. Major fatty acids of type strains of type species of both genera are C14 : 0, C14 : 0 3-OH/iso-C16 : 1 I, C16 : 1ω7c and C16 : 0. The two genera are clearly separated by phenotype from each other and from existing genera of the family Pasteurellaceae . The type strain of Mesocricetibacter intestinalis is HIM 933/7T ( = Kunstyr 246/85T = CCUG 28030T = DSM 28403T) while the type strain of Cricetibacter osteomyelitidis is HIM943/7T ( = Kunstyr 507/85T = CCUG 36451T = DSM 28404T).

Sign in / Sign up

Export Citation Format

Share Document