Production of Cadaverine in Recombinant Corynebacterium Glutamicum Overexpressing Lysine Decarboxylase (ldcC) and Response Regulator dr1558

2021 ◽  
Author(s):  
Soong-bin Kang ◽  
Jong-il choi
Keyword(s):  
Corynebacterium Glutamicum ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Deinococcus Radiodurans ◽  
Response Regulator ◽  
Fold Increase ◽  
Lysine Biosynthesis ◽  
Lysine Decarboxylase ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Polymerase Chain

Abstract In this study, the response regulator DR1558 from Deinococcus radiodurans was overexpressed in recombinant Corynebacterium glutamicum with lysine decarboxylase (ldcC). The recombinant C. glutamicum strain overexpressing dr1558 and ldcC produced 5.9 g/L of cadaverine by flask cultivation, whereas the control strain overexpressing only ldcC produced 4.5 g/L of cadaverine. To investigate the mechanism underlying the effect of DR1558, the expression levels of genes related to central metabolism and lysine-biosynthesis were analyzed by quantitative-real time polymerase chain reaction. The results showed that phosphoenolpyruvate carboxykinase (pck) was downregulated, and pyruvate kinase (pyk) and other lysine biosynthesis genes were upregulated. Furthermore, in fed-batch fermentation, C. glutamicum coexpressing dr1558 produced 25.14 g/L of cadaverine, a 1.25-fold increase in concentration relative to the control. These results suggested that the heterologous expression of dr1558 may improve the production of biorefinery products by recombinant C. glutamicum.

Co-Expression of Threonine Dehydratase and Acetolactate Synthase in Escherichia Coli for L-Isoleucine Production

2014 ◽  
Vol 989-994 ◽  
pp. 997-1002 ◽  
Author(s):  
Jian Wang ◽  
Jia Kai Sun ◽  
Qing Yang Xu
Keyword(s):  
Escherichia Coli ◽  
Corynebacterium Glutamicum ◽  
Carbon Flux ◽  
Batch Fermentation ◽  
Acetolactate Synthase ◽  
Fed Batch ◽  
Threonine Dehydratase ◽  
E Coli ◽  
Fed Batch Fermentation ◽  
Fermentation Period

Metabolic engineering ofCorynebacterium glutamicumhas sought to divert carbon into L-isoleucine. However, the fermentation period of this strain is long. TheC.glutamicumYILW strain (LeuL, AHVr, SGr, Leu-MEr) was previously derived by repeated compound mutagenesis which could accumulate 20.2 g/L L-isoleucine in a 5-L jar fermentor. Overexpression of the threonine dehydratase gene (ilvA) fromCorynebacterium glutamicumYILW and coexpression of threonine dehydratase and acetolactate synthase (ilvBN) from it were employed to divert carbon flux toward L-isoleucine. The strainE. coliTRFC with the expression ofilvA could accumulate L-isoleucine of 6.8 g/L without accumulation of any L-threonine by fed-batch fermentation in a 5-L jar fermentor. However, the production of L-isoleucine by the strainE.coliTRFC with the co-expression ofilvA andilvBN was decreased by 19.1%, and the production of L-valine was increased by 40% compared with that ofE. coliTRFC with the expression ofilvA.

scholarly journals Enhanced Cadaverine Production by Recombinant Corynebacterium Glutamicum with Response Regulator DR1558 at low pH Conditions

2021 ◽  
Author(s):  
Soong-bin Kang ◽  
Jong-Il Choi
Keyword(s):  
Cell Growth ◽  
Organic Acids ◽  
Corynebacterium Glutamicum ◽  
Response Regulator ◽  
Batch Cultivation ◽  
Low Ph ◽  
Fed Batch ◽  
Acidic Conditions ◽  
Fed Batch Cultivation ◽  
Stress Defense

Abstract Background: Corynebacterium glutamicum is used industrially to produce various bio-based organic acids. However, it is often cultivated under abiotic stress conditions, such as low pH, which can reduce both cell growth and the yield of the target compound. Here, a response regulator from Deinococcus radiodurans, DR1558, was introduced into a recombinant C. glutamicum strain expressing lysine decarboxylase (cadA) to enhance cadaverine production at acidic pHs.Results: During batch cultivation under acidic conditions, 6.4 g/L of cadaverine was produced by the recombinant C. glutamicum strain expressing cadA and dr1558; this yield was 1.7-fold higher than that produced by a recombinant C. glutamicum strain expressing only cadA. Transcriptional analysis revealed altered expression levels of stress defense- and cadaverine biosynthesis-related genes in the recombinant C. glutamicum strain expressing dr1558. During fed-batch cultivation, the recombinant C. glutamicum strain expressing cadA and dr1558 showed a 2.4-fold increase in cadaverine production compared to that produced by the recombinant C. glutamicum strain expressing only cadA. The cell growth of C. glutamicum expressing both cadA and dr1558 increased markedly during fed-batch cultivation at acidic pH.Conclusion: These results indicated that the response regulator dr1558 altered the expression of genes involved in metabolic pathways and stress defense mechanisms in C. glutamicum. Furthermore, C. glutamicum expressing the D. radiodurans dr1558 can be used to produce bio-based organic acids by fermentation in processes requiring acidic conditions.

scholarly journals Improved Mannanase Production from Penicillium occitanis by Fed-Batch Fermentation Using Acacia Seeds

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Monia Blibech ◽  
Raoudha Ellouz Ghorbel ◽  
Fatma Chaari ◽  
Ilyes Dammak ◽  
Fatma Bhiri ◽  
...  
Keyword(s):  
Batch Fermentation ◽  
Shake Flask ◽  
Fold Increase ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Culture Process ◽  
Coconut Meal ◽  
Penicillium Occitanis ◽  
Shake Flask Fermentation

By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase production using acacia seeds powder as inducer substrate showed the suitability of this culture process for industrial-scale development.

Glycerol Synthesis Attenuation ofCandida glycerinogenesin Fed-batch Fermentation

2012 ◽  
Vol 18 (5) ◽  
pp. 791
Author(s):  
Xiaoyun DING ◽  
Bin ZHUGE ◽  
Huiying FANG ◽  
Hong ZONG ◽  
Xiaoxiao LIU ◽  
...  
Keyword(s):  
Batch Fermentation ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Glycerol Synthesis
Sign in / Sign up

Export Citation Format

Share Document