scholarly journals Fluorometric Assessment of Sulfhydryl Oxidase Activity: Optimization by Response Surface Methodology

2021 ◽  
Author(s):  
mahmoud hussein hadwan ◽  
Abdul Razzaq S. Alsalman ◽  
Lamia A. Almashhedy ◽  
Abdulsamie H. Alta'ee ◽  
Asad M. Hadwan
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Oxidase Activity ◽  
Colorimetric Method ◽  
Current Method ◽  
Biological Tissues ◽  
The Novel ◽  
Sulfhydryl Oxidase ◽  
Peroxidase Mimic ◽  
Analytical Recovery

Abstract Sulfhydryl oxidase was studied using a spectrofluorometric assay. The current protocol operates by using a combination of hemoglobin (HB) and hematin (HT) as a peroxidase mimic to catalyze the H2O2-dependent oxidation of thiamine. The response surface methodology is used to optimize the new method (RSM). The current method is very accurate, sensitive, and linear up to 200 IU. When compared to the colorimetric method, the method produced a satisfactory correlation. The novel protocol is being used to evaluate asthenospermic patients' and fertile men's seminal SHO activity. The current protocol was used to determine reference values for seminal sulfhydryl oxidase activity. Due to the fact the newly developed spectrofluorometric method is more sensitive and precise than other colorimetric methods, and because thiamine is less expensive than other types of probes used in colorimetric and spectrofluorometric methods, it is likely to find widespread use among scientists studying SHO activity in biological tissues. The present method's analytical recovery yielded high specific findings.

scholarly journals Fluorometric Assessment of Sulfhydryl Oxidase Activity: Optimization by Response Surface Methodology

2021 ◽  
Author(s):  
Mahmoud H. Hadwan ◽  
AbdulRazzaq S. Alsalman ◽  
Lamia A. Almashhedy ◽  
Abdulsamie H. Altaee ◽  
Asad M. Hadwan
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Oxidase Activity ◽  
Sulfhydryl Oxidase ◽  
Activity Optimization

scholarly journals Optimization of the Novel Bisulfite Steeping Method by Response Surface Methodology for Natural Starch Synthesis From Oil Palm Trunk Biomass

2020 ◽  
Author(s):  
Zaber Ahmed ◽  
Mohd Suffian Yusoff ◽  
Nurul Hana Mokhtar Kamal ◽  
Hamidi Abdul Aziz
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Palm Oil ◽  
Pure Water ◽  
Starch Synthesis ◽  
Type Model ◽  
The Novel ◽  
Mixing Ratio ◽  
Ultra Pure Water ◽  
Oil Export

Abstract Malaysia is the 2nd largest in palm oil export, and after overcoming economic age (average 25 years), the palm oil trees entail to replace usually. Therefore, a massive quantity of palm oil trunk biomass, containing a significant amount of starch, is available as bio-waste annually. The efficient extraction of this starch (carbohydrate polymer) would be worthwhile concerning the environment, economy, conversion of biowaste to bioresources, and waste dumping challenges. Central composite design executed an experimental model design, evaluated the impacts of process variables and their interaction through response surface methodology to optimize the novel bisulfite steeping method for starch synthesis. Design-Expert software performed the data analysis. The developed quadratic models for four factors (Strength of Sodium bisulfite solution, steeping hour, mixing ratio with the bisulfite solution and ultra-pure water) and one response (%Yield), demonstrated that a significant starch yield (13.54%) is achievable utilizing 0.74% bisulfite solution, 5.6 steeping hours, for 1.6 and 0.6 mixing ratio with the bisulfite solution and ultra-pure water respectively. Experimental outcomes were quite consistent with the predicted model, which eventually sustains the significance of this method. Malvern Zetasizer test revealed a bimodal granular distribution for starch, with 7.15µm of hydrodynamic size. Starch morphology was determined by scanning electron microscopy. X-ray diffraction investigation exhibits an A-type model, specifying persistent characteristics of extracted starch.

scholarly journals Pelletization of Sunflower Seed Husks: Evaluating and Optimizing Energy Consumption and Physical Properties by Response Surface Methodology (RSM)

Processes ◽  
2019 ◽  
Vol 7 (9) ◽  
pp. 591 ◽  
Author(s):  
Xuyang Cui ◽  
Junhong Yang ◽  
Xinyu Shi ◽  
Wanning Lei ◽  
Tao Huang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Energy Consumption ◽  
Moisture Content ◽  
Physical Properties ◽  
Response Surface ◽  
Sunflower Seed ◽  
Biomass Energy ◽  
Raw Material ◽  
The Novel ◽  
Seed Husk

Pelletization is a significant approach for the efficient utilization of biomass energy. Sunflower seed husk is a common solid waste in the process of oil production. The novelty of this study was to determine the parameters during production of a novel pellet made from sunflower seed husk. The energy consumption (W) and physical properties (bulk density (BD) and mechanical durability (DU)) of the novel pellet were evaluated and optimized at the laboratory by using a pelletizer and response surface methodology (RSM) under a controlled moisture content (4%–14%), compression pressure (100–200 MPa), and die temperature (70–170 °C). The results show that the variables of temperature, pressure, and moisture content of raw material are positively correlated with BD and DU. Increasing the temperature and moisture content of raw materials can effectively reduce W, while increasing the pressure has an adverse effect on W. The optimum conditions of temperature (150 °C), pressure (180 MPa), and moisture content (12%) led to a BD of 1117.44 kg/m3, DU of 98.8%, and W of 25.3 kJ/kg in the lab. Overall, although the nitrogen content was slightly high, the novel manufactured pellets had excellent performance based on ISO 17225 (International Organization for Standardization of 17225, Geneva, Switzerland, 2016). Thus, sunflower seed husk could be considered as a potential feedstock for biomass pelletization.

Response surface methodology approach for optimization of simultaneous dye and metal ion ultrasound-assisted adsorption onto Mn doped Fe3O4-NPs loaded on AC: kinetic and isothermal studies

2015 ◽  
Vol 44 (33) ◽  
pp. 14707-14723 ◽  
Author(s):  
Arash Asfaram ◽  
Mehrorang Ghaedi ◽  
Alireza Goudarzi ◽  
Maryam Rajabi
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Metal Ion ◽  
Ultrasonic Power ◽  
The Novel ◽  
Fe3o4 Nps ◽  
Ultrasound Assisted ◽  
Mn Doped

Ultrasonic power was investigated as a dispersion and mixing tool to accelerate the adsorption of SO, MB, Pb2+ ions and Cr3+ ions onto the novel Fe3O4-NPs-AC composite.

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