Cholesterol oxidase activity of a new isolated bacterium and optimization of culture medium using response surface methodology

2011 ◽  
Vol 22 ◽  
pp. S88
Author(s):  
Azam Safari ◽  
Abdollah Ghasemian ◽  
Zahra Moradpour ◽  
Younes Ghasemi
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Oxidase Activity ◽  
Culture Medium ◽  
Cholesterol Oxidase

scholarly journals Production of nigericin and niphimycin by soil isolate Streptomyces sp. MS1: Anti-Candida bioassay guided response surface methodology for the optimized culture medium

2017 ◽  
Vol 15 (1) ◽  
pp. 1-16
Author(s):  
Marija Mojicevic ◽  
Jovana Grahovac ◽  
Milos Petkovic ◽  
Ivan Vuckovic ◽  
Jelena Dodic ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Culture Medium ◽  
Genus Streptomyces ◽  
Physiological And Biochemical Characteristics ◽  
Antifungal Antibiotics ◽  
Rdna Sequencing ◽  
Soil Isolate ◽  
Physiological And Biochemical

Anti-Candida bioassay guided optimization of the culture medium was used in order to enhance the antifungal activity of the soil isolate MS1. Its morphological, physiological and biochemical characteristics, as well as 16S rDNA sequencing, assigned an MS1 isolate to genus Streptomyces. Optimization of the culture medium was achieved by experimental factorial design and response surface methodology. Maximal antifungal components production was obtained with starch, soybean meal and phosphates content of 40.52, 5.10 and 2.21 g/L, respectively. Chromatography and 1H and 13CNMR spectroscopy were employed for purification and structural characterization of antifungal antibiotics concurrently produced by this strain. These antibiotics were identified as polyether carboxylic antibiotic nigericin and guanidyl-polyol macrolide, niphimycin.

Schizophyllan production by newly isolated fungus Schizophyllum commune IBRC-M 30213: optimization of culture medium using response surface methodology

2017 ◽  
Vol 68 (1) ◽  
pp. 47-62 ◽  
Author(s):  
Aref Mohammadi ◽  
Seyed Abbas Shojaosadati ◽  
Hajar Jamshidian Tehrani ◽  
Seyed Mohammad Mousavi ◽  
Tayebeh Saleh ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Culture Medium ◽  
Schizophyllum Commune

scholarly journals Production of β-Glucosidase from a Newly IsolatedAspergillusSpecies Using Response Surface Methodology

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Pilanee Vaithanomsat ◽  
Molnapat Songpim ◽  
Taweesiri Malapant ◽  
Akihiko Kosugi ◽  
Warunee Thanapase ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ammonium Sulfate ◽  
Yeast Extract ◽  
Culture Medium ◽  
Specific Activity ◽  
Ph Value ◽  
Sulfate Concentration ◽  
Extract Concentration ◽  
Fermentation Parameters

A newly isolated fungusAspergillus nigerSOI017 was shown to be a good producer of β-glucosidase from all isolated fungal strains. Fermentation condition (pH, cellobiose concentration, yeast extract concentration, and ammonium sulfate concentration) was optimized for producing the enzyme in shake flask cultures. Response surface methodology was used to investigate the effects of 4 fermentation parameters (yeast extract concentration, cellobiose concentration, ammonium sulfate concentration, and pH) on β-glucosidase enzyme production. Production of β-glucosidase was most sensitive to the culture medium, especially the nitrogen source yeast extract. The optimized medium for producing maximum β-glucosidase specific activity consisted of 0.275% yeast extract, 1.125% cellobiose, and 2.6% ammonium sulfate at a pH value of 3.

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