In-Vivo Comparative Virulence of Different White Spot Syndrome Virus Isolates In Penaeus Vannamei And Whole Genome Comparison Analysis

Abstract White Spot Syndrome Virus (WSSV) infects several economically important aquacultural species, causing significant losses to the industry. This virus belongs to the Nimaviridae family, and has a dsDNA genome ranging from 257 to 309 kb (more than 20 isolate genomes fully sequenced and published to date). Multiple routes of infection could be the cause of the high virulence and mortality rates detected in shrimp species. In particular, Penaeus vannamei, differences in isolate virulence have been observed, along with controversy over whether deletions or insertions are associated with gain or loss of virulence.The pathogenicity of three isolates from three locations in Mexico (two from Sinaloa: 'CIAD', and 'Angostura', and one from Sonora: 'Sonora') was evaluated in vivo in white shrimp (P. vannamei) infection assays. Differences were observed in the mortality rate of shrimp among the three isolates, with the Sonora isolate being the most virulent. Subsequently, the complete WSSV genomes were sequenced in depth from the tissues of infected shrimp, and assembled in reference to the genome of isolate CN01 (KT995472), identifying genome sizes for Angostura and Sonora of 289,350 bp and 288,995 bp, respectively. Where three deletion zones were identified compared to CN01 comprising 15 genes, including three envelope proteins VP41A, VP52A and VP41B, one non-structural protein ICP35 and 11 others encoding proteins whose function is currently unknown. In addition, five genes (wsv129, wsv178, wsv204, wsv249 and wsv497) show a modified number of repeat motifs. The main implications and possible effects on viral infection of these modifications are discussed.

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In Vivo Titration and Development of a Challenge Model for White Spot Syndrome Virus (WSSV) in Pacific White Shrimp (Litopeneus vannamei)

10.31274/ans_air-180814-1052 ◽

2011 ◽

Author(s):

Duan S. Loy ◽

Lyric C. Bartholomay ◽

D.L. Hank Harris

Keyword(s):

White Spot Syndrome Virus ◽

Pacific White Shrimp ◽

White Spot ◽

White Shrimp ◽

White Spot Syndrome

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Screening and characterization of antiviral compounds from Enteromorpha flexuosa against white spot syndrome virus (WSSV) and its in vivo influence on Indian white shrimp Fenneropenaeus indicus

Aquaculture International ◽

10.1007/s10499-014-9798-y ◽

2014 ◽

Vol 23 (1) ◽

pp. 65-80 ◽

Cited By ~ 5

Author(s):

S. Velmurugan ◽

N. Jerin ◽

M. Michael Babu ◽

F. Bindhu ◽

S. Albin Dhas ◽

...

Keyword(s):

White Spot Syndrome Virus ◽

White Spot ◽

White Shrimp ◽

Fenneropenaeus Indicus ◽

Antiviral Compounds ◽

Enteromorpha Flexuosa ◽

White Spot Syndrome

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Identification of antigenic domains and peptides from VP15 of white spot syndrome virus and their antiviral effects in Marsupenaeus japonicus

Scientific Reports ◽

10.1038/s41598-021-92002-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Jirayu Boonyakida ◽

Jian Xu ◽

Jun Satoh ◽

Takafumi Nakanishi ◽

Tohru Mekata ◽

...

Keyword(s):

Survival Rate ◽

White Spot Syndrome Virus ◽

Expression System ◽

Penaeid Shrimp ◽

White Spot ◽

Marsupenaeus Japonicus ◽

Structural Protein ◽

E Coli ◽

White Spot Syndrome

AbstractWhite spot syndrome virus (WSSV) is one of the most devastating pathogens in penaeid shrimp and can cause massive damage in shrimp aquaculture industries. Previously, the WSSV structural protein VP15 was identified as an antigenic reagent against WSSV infections. In this study, we truncated this protein into VP15(1–25), VP15(26–57), VP15(58–80), and VP15(1–25,58–80). The purified proteins from the E. coli expression system were assayed as potential protective agents in Kuruma shrimp (Marsupenaeus japonicus) using the prime-and-boost strategy. Among the four truncated constructs, VP15(26–57) provided a significant improvement in the shrimp survival rate after 20 days of viral infection. Subsequently, four peptides (KR11, SR11, SK10, and KK13) from VP15(26–57) were synthesized and applied in an in vivo assay. Our results showed that SR11 could significantly enhance the shrimp survival rate, as determined from the accumulated survival rate. Moreover, a multiligand binding protein with a role in the host immune response and a possible VP15-binding partner, MjgC1qR, from the host M. japonicus were employed to test its binding with the VP15 protein. GST pull-down assays revealed that MjgC1qR binds with VP15, VP15(26–57), and SR11. Taken together, we conclude that SR11 is a determinant antigenic peptide of VP15 conferring antiviral activity against WSSV.

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Identification of Antigenic Domains and Peptides from VP15 of White Spot Syndrome Virus and their Antiviral Effects in Marsupenaeus Japonicus

10.21203/rs.3.rs-143155/v1 ◽

2021 ◽

Author(s):

Jirayu Boonyakida ◽

Jian Xu ◽

Jun Satoh ◽

Takafumi Nakanishi ◽

Toru Mekata ◽

...

Keyword(s):

Survival Rate ◽

White Spot Syndrome Virus ◽

Expression System ◽

Penaeid Shrimp ◽

White Spot ◽

Marsupenaeus Japonicus ◽

Structural Protein ◽

E Coli ◽

White Spot Syndrome

Abstract White spot syndrome virus (WSSV) is one of the most devastating pathogens in penaeid shrimp and can cause massive damage in shrimp aquaculture industries. Previously, the WSSV structural protein VP15 was identified as an antigenic reagent against WSSV infections. In this study, we truncated this protein into VP15(1−25), VP15(26−57), VP15(58−80), and VP15(1−25,58−80). The purified proteins from the E. coli expression system were assayed as potential protective agents in Kuruma shrimp (Marsupenaeus japonicus) using the prime-and-boost strategy. Among the four truncated constructs, VP15(26−57) provided a significant improvement in the shrimp survival rate after 20 days of viral infection. Subsequently, four peptides (KR11, SR11, SK10, and KK13) from VP15(26−57) were synthesized and applied in an in vivo assay. Our results showed that SR11 could significantly enhance the shrimp survival rate, as determined from the accumulated survival rate. Moreover, a multiligand binding protein with a role in the host immune response and a possible VP15-binding partner, MjgC1qR, from the host M. japonicus were employed to test its binding with the VP15 protein. GST pull-down assays revealed that MjgC1qR binds with VP15, VP15(26−57), and SR11. Taken together, we conclude that SR11 is a determinant antigenic peptide of VP15 conferring antiviral activity against WSSV.

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