Rapid Propagation System in Vitro of Medicinal Plant Cynanchum Atratum Bunge

2021 ◽  
Author(s):  
P Chang ◽  
GF Dong ◽  
MF Li ◽  
YH Zhang ◽  
Yumei Dong
Keyword(s):  
Urinary Infection ◽  
Axillary Bud ◽  
Adventitious Bud ◽  
Ms Medium ◽  
Adventitious Buds ◽  
Axillary Bud Proliferation ◽  
Rapid Propagation ◽  
Wild Resources ◽  
Culture Technology

Abstract As a traditional Chinese medicinal material, Cynanchum atratum Bunge has been widely used in traditional Chinese medicine for its treatment of abscesses, acute urinary infection and hectic fever.Thus, wild resources of it have been endangered by overharvesting. Plant tissue culture technology is an important measure to protect wild resources of medicinal plants, including C. atratum. Therefore, a fast and efficient propagation system of C. atratum through axillary bud proliferation pathwayhas been established. Through axillary bud proliferation, the medium [MS+sucrose 30 g/L+Agar 7 g/L+NAA 0.2 mg/l+IBA 1.5mg/l+KT 0.5 mg/l] can effectively proliferate adventitious buds, and the induction rate was 100 %, proliferation coefficient could reach 8.56. MS medium was used to induce adventitious bud rooting, with rooting rate of 98% and no callus. The highest survival rate was 90% when the ratio of grass mud pond and orchard red soil was 1:1. To our knowledge this is the first report of rapid propagation system in C. atratum, it achieve rapid reproduction of C. atratum.

scholarly journals Callus induction and adventitious bud differentiation of Cyclocodon lancifolius (Roxb.) Kurz

2020 ◽  
Vol 98 (4) ◽  
pp. 534-544
Author(s):  
Yin-Kai Xi ◽  
Ye Wang ◽  
Biao Zeng ◽  
Heng-Yu Huang ◽  
Wu-Dei Yang
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Culture Method ◽  
Adventitious Shoot ◽  
Adventitious Bud ◽  
Ms Medium ◽  
Regeneration System ◽  
Edible Plant ◽  
Wild Resources

Background: Cyclocodon lancifolius (Roxb.) Kurz is a perennial medicinal and edible plant with a huge potential economic value. The wild resources of this plant are gradually scarce by the serious destruction of the habitat and the limitations of sexual reproduction. This is the first attempt to establish an in vitro reproductive system for the species. Hypothesis: The suitable plant regulator types and its mass concentration range, combined with the explants, can induce the development of plants at various stages. We expected to establish an in vitro regeneration system of C. lancifolius based on these factors. Species studied: Cyclocodon lancifolius Study site and years of study: Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, from 2017 to 2019. Methods: The plant regeneration of C. lancifolius was established by single factor, L9 (34) orthogonal and complete combination experiments. Results: Stem segments were the best explants for callus induction on MS medium containing 0.05 mg·L-1 KT, 0.5 mg·L-1 6-BA and 0.5 mg·L-1 NAA. MS medium with 0.2 mg·L-1 TDZ and MS medium were used alternately as the culture method to conduct differentiation and proliferation of adventitious shoot. The optimal protocol for the rooting was MS medium combined with 0.1 mg·L-1 6-BA and 1.0 mg·L-1 NAA. Conclusions: A rapid propagation system of C. lancifolius was established which provided a possible solution for the protection of wild resources and artificial cultivation.

scholarly journals Induction of callus and adventitious shoots on epicotyl and hypocotyl segments of cumaru (Dipteryx odorata)

2018 ◽  
Vol 9 (3) ◽  
pp. 475-480
Author(s):  
Paulo Tarso Barbosa Sampaio ◽  
Lyana Silva Jardim ◽  
Ariel Dotto Blind ◽  
Flavio Mauro Souza Bruno
Keyword(s):  
Native Species ◽  
Callus Formation ◽  
Adventitious Shoots ◽  
Ms Medium ◽  
Adventitious Buds ◽  
Clonal Multiplication ◽  
Benefit Ratio ◽  
Dipteryx Odorata ◽  
Hypocotyl Segments

Somatic embryogenesis from callus induced in epicotyl and hypocotyl segments can be viable native species in order to better -benefit ratio costs, and rates of clonal multiplication. In this sense, two trials were established to induce callus and adventitious buds on hypocotyl and epicotyl segments of cumaru bean seedlings germinated in vitro in different concentrations and combinations of growth regulators. At first, we used the MS medium supplementwith ANA (0.0, 1.5 mg.L-1) and TDZ (0.0, 4.0 and 8.0 mg.L-1) distributed in factorial 2 x 3 x 2 (x auxin cytokinin x explant) with eight replications. In the second, it was used the WPM medium supplemented with BAP (2.0 mg L-1) and plus 2,4-D (2.0 and 4.0 mg L-1) in a factorial 2 x 2 (auxin x explant) with 15 repetitions each. They were evaluating callus formation and the average number of adventitious shoots during the period of 90 days. The results indicated that the highest average for callus formation was observed when the explants were subjected to concentrations of 8.0 mg L-1 TDZ combined with 1.5 mg L-1 ANA in MS medium. For the formation of buds, the WPM medium plus 2.0 mg L-1 2,4-D in the second experiment, induced higher number of shoots, being significant the use of auxin, and its interaction with the type of explant.

scholarly journals Ethnobotany and In vitro regeneration of Acorus calamus L. (Acoraceae): a high valued medicinal and economic plant

2017 ◽  
Vol 6 (2) ◽  
pp. 1566
Author(s):  
Jintu Sarma* ◽  
Pratibha Sharma
Keyword(s):  
In Vitro Regeneration ◽  
Shoot Proliferation ◽  
Culture Technique ◽  
Axillary Bud ◽  
Cow Dung ◽  
Acorus Calamus ◽  
Economic Plant ◽  
Ms Medium ◽  
Important Medicinal Plant

Acorus calamus L. is a species of enormous medicinal and economic importance. In vitro propagation of this plant was achieved using axillary bud explant. In the present investigation, naturally grown axillary bud and rhizome explants were cultured on standard MS and B5 medium supplemented with different concentration and combination of cytokinines and auxines. The best shoot proliferation was observed in MS medium containing Kn (1.0mg/l) +IBA (0.5mg/l) with 3.33±0.58 nos. of Shoots, 7.33±0.58 nos. of roots and 15.33±0.58 nos. leaves. In B5 medium best results found in Kn (1.5mg/) + NAA (1.0mg/l) with 2.67±0.58 nos. shoots, 3.67±0.58 nos. of roots and11.67±0.58 nos. of leaves. They were then transplanted in soil: sand: cow dung mixture (1:1:2) and kept in shade for 4 to 5 weeks and then transferred to field for one month. Survival rate was found 80 % in MS medium and 100 % in B5 medium. The present investigation was carried out with a view to standardize an in vitro culture technique for mass propagation of this important medicinal plant species and was found successful.

scholarly journals Regeneration of Pruatjan (Pimpinella pruatjan Molk): Axillary Bud Proliferation and Encapsulation

2016 ◽  
Vol 2 (2) ◽  
pp. 68 ◽  
Author(s):  
Ika Roostika ◽  
Ireng Darwati ◽  
Ika Mariska
Keyword(s):  
Axillary Bud ◽  
Growth Chamber ◽  
Axillary Bud Proliferation

<p class="p1">Purwoceng (<em>Pimpinella alpina </em>KDS atau <em>Pimpinella pruatjan </em>Molk.) merupakan tanaman obat asli Indonesia yang terancam punah. Akarnya dapat dimanfaatkan sebagai obat afrodisiak, diuretik, dan tonik. Teknik kultur <em>in vitro </em>merupakan teknologi alternatif yang dapat diterapkan untuk konservasi dan perbanyakan tanaman tersebut. Mikropropagasi telah dilakukan melalui jalur organogenesis dengan proliferasi tunas aksilar dan enkapsulasi. Penelitian dilakukan di Laboratorium Kultur Jaringan BB-Biogen, Bogor mulai tahun 2004 hingga 2005. Penelitian ini terbagi atas empat percobaan, yaitu (1) optimasi lingkungan tumbuh kultur, (2) optimasi formulasi media untuk proliferasi tunas aksilar dan enkapsulasi tunas aksilar, (3) induksi perakaran, dan (4) aklimatisasi. Kondisi lingkungan kultur yang optimum adalah di <em>growth chamber </em>dengan suhu 9<span class="s1">o</span>C dan intensitas cahaya 1000 lux. Formulasi media terbaik untuk proliferasi tunas aksilar adalah media DKW dengan penambahan BA 4 ppm dengan eksplan berupa tunas tanpa daun. Penggunaan arginin 100 ppm lebih baik daripada glutamin 100 ppm dan modifikasi vitamin (mioinositol 100 ppm dan thiamine-HCl 1 ppm). Pada media yang sama, pertumbuhan tunas aksilar terenkapsulasi juga paling baik dan tunas tersebut dapat menembus kapsul alginat setelah 4 minggu dalam periode <em>in vitro </em>(85%). Penggunaan NAA 1,0 ppm menginduksi perakaran paling cepat (40 hari) dengan persentase perakaran paling tinggi (100%). Vermikulit bertekstur kasar paling baik untuk aklimatisasi tunas aksilar terenkapsulasi sedangkan arang sekam paling baik untuk aklimatisasi planlet.</p>

scholarly journals Elimination of In vitro Contamination, Shoot Multiplication, and Ex vitro Rooting of Aglaonema

HortScience ◽  
2007 ◽  
Vol 42 (3) ◽  
pp. 629-632 ◽  
Author(s):  
W.L. Chen ◽  
D.M. Yeh
Keyword(s):  
Shoot Multiplication ◽  
Ex Vitro Rooting ◽  
Axillary Bud ◽  
Ms Medium ◽  
Ex Vitro

Elimination of in vitro contamination and shoot multiplication were studied with Aglaonema Schott ‘White Tip’. Apparently, contamination was reduced, but explants browned when 200 mg·L−1 streptomycin was used as either a pretreatment or incorporated into the medium. Reduced occurrence of contamination and browning was achieved in axillary bud explants excised from the stock plants that had not been watered for 2 months. Six shoots per explant elongated normally in Murashige and Skoog (MS) medium containing 30 μm benzylaminopurine (BA). MS medium containing 20 μm thidiazuron (TDZ) also resulted in six shoots per explant, but these shoots failed to extend beyond a rosette. Only microcuttings from 30 μm BA treatment were used for the ex vitro rooting trial, and indole-3-butytric acid (IBA) at 9.8 or 19.7 mm applied to the base of the microcuttings resulted in 100% ex vitro rooting and the longest roots.

scholarly journals An Efficient and Easy Micro-propagation of Reseda Lutea (Resedaceae) a Rare and Medicinally Valuable Plant of Saudi Arabia

2021 ◽  
Author(s):  
FAHAD AL-QURAINY ◽  
SALEH ALANSI ◽  
SALIM KHAN ◽  
MOHAMMAD NADEEM ◽  
AREF AL-SHAMERI ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Saudi Arabia ◽  
Peat Moss ◽  
Ms Medium ◽  
Root Initiation ◽  
Adventitious Buds ◽  
Micro Propagation ◽  
Complete Procedure

Abstract The goal of this work was to look at the propagation of Reseda lutea L. by organogenesis in tissue culture. Explants from in vitro grown seedlings were taken from the axillary bud. After seven days of culture on MS medium supplemented with 1.0 mg/l BA, the adventitious buds developed. After three weeks of culturing on MS medium supplemented with 1.5 mg/l BA, the maximum multiplication of shoots (16.12 shoots/explant) was discovered, with an average (7.37 cm) shoots/explant. These shoots were sub-cultured on MS media with varying concentrations of NAA and IBA for root initiation. The MS medium combined with IBA produced the greatest percentage of root development (92%) and the greatest number of roots (7.37 roots/plant). In MS media supplemented with 0.5 NAA, the longest roots (3.08 cm) were found. After 17 days in a glasshouse, the plantlets were acclimatized in pots containing Peat moss and pearlite, 98 percent of the plantlets were acclimatized. To get a plant in a pot, the complete procedure took about 75 days. The technique proposed could aid in the preservation of the plant both in vivo and in vitro.

Rapid in vitro Propagation of Dodonaea viscosa (Sapindaceae) Through Axillary Bud Multiplication and Indirect Organogenesis

2015 ◽  
Vol 22 (1) ◽  
pp. 33-36
Author(s):  
A. Benniamin ◽  
G. Jothi ◽  
M. Sundari
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
High Rate ◽  
Axillary Bud ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Induction ◽  
Dodonaea Viscosa ◽  
Indirect Organogenesis

Protocols of axillary bud multiplication were established for Dodonaea viscosa (Sapindaceae). Murashig & Skoog’s medium with 0.2 mg/l BAP with 0.01 Naphthalene acetic acid induced high rate of shoot induction and an average of five shoots per node. Subsequent culture enhanced the number of shoots. Callus initiated from the basal cut end explants differentiated in to more than 10 shoots on MS Medium with 0.4mg/l Benzylaminopurine and 0.02mg/l Indole Butric Acid. Eighty per cent of the rooted shoots survived when transferred to greenhouse and subsequently to the field.

scholarly journals Induction of Adventitious buds from stem explants in Aoectochilus formosanus

2019 ◽  
Vol 131 ◽  
pp. 01131
Author(s):  
Yingbin Xue ◽  
Fengyi Xiao ◽  
Xiaohao Li ◽  
Huamei Chen ◽  
Gangshun Rao ◽  
...  
Keyword(s):  
Culture Conditions ◽  
Adventitious Bud ◽  
Naphthaleneacetic Acid ◽  
Stem Explants ◽  
Ms Medium ◽  
Adventitious Buds ◽  
High Concentration ◽  
Diethyl Aminoethyl Hexanoate ◽  
Adventitious Bud Regeneration ◽  
Stem Segments

In order to explore the optimal culture conditions for adventitious bud regeneration of stem explants in Aoectochilus formosanus, the stem segments from the sterile seedlings were used as explants, and different concentrations of diethyl aminoethyl hexanoate (DA-6), kinetin (KT), Cu2+ and glutamine (Gln) were separately added into MS medium containing 6-benzylaminopurine (6-BA) and naphthaleneacetic acid (NAA), and the induction rate and the induction multiple of adventitious buds were recorded and analyzed. The results showed that the regeneration of adventitious buds could be promoted, when 2 mg/L DA-6, 0.4 mg/L KT and 15 mg/L Gln were added in mediums. However, the effect of Cu2+ on the regeneration of adventitious buds in A. formosanus was enhanced by low concentration and suppressed by high concentration, and the best concentration of Cu2+ was 5 mg/L.

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