Human Mammary Epithelial Cell Transformation by Rho GTPase through a Novel Mechanism

2008 ◽  
Author(s):  
Vimia Band
Keyword(s):  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Rho Gtpase ◽  
Cell Transformation ◽  
Human Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Human Mammary Epithelial

scholarly journals Human mammary epithelial cell transformation through the activation of phosphatidylinositol 3-kinase

Cancer Cell ◽  
2003 ◽  
Vol 3 (5) ◽  
pp. 483-495 ◽  
Author(s):  
Jean J Zhao ◽  
Ole V Gjoerup ◽  
Romesh R Subramanian ◽  
Yuan Cheng ◽  
Wen Chen ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Cell Transformation ◽  
Human Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Phosphatidylinositol 3 Kinase ◽  
Human Mammary Epithelial ◽  
Phosphatidylinositol 3

scholarly journals Mechanisms of HERV-K (HML-2) Transcription during Human Mammary Epithelial Cell Transformation

2017 ◽  
Vol 92 (1) ◽  
Author(s):  
Meagan Montesion ◽  
Neeru Bhardwaj ◽  
Zachary H. Williams ◽  
Charlotte Kuperwasser ◽  
John M. Coffin
Keyword(s):  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Promoter Activity ◽  
Cell Transformation ◽  
Human Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Human Mammary Epithelial ◽  
Tumorigenic Cells ◽  
Ltr Promoter

ABSTRACTIncreasing evidence suggests that repetitive elements may play a role in host gene regulation, particularly through the donation of alternative promoters, enhancers, splice sites, and termination signals. Elevated transcript expression of the endogenous retrovirus group HERV-K (HML-2) is seen in many human cancers, although the identities of the individual proviral loci contributing to this expression as well as their mechanisms of activation have been unclear. Using high-throughput next-generation sequencing techniques optimized for the capture of HML-2 expression, we characterized the HML-2 transcriptome and means of activation in anin vitromodel of human mammary epithelial cell transformation. Our analysis showed significant expression originating from 15 HML-2 full-length proviruses, through four modes of transcription. The majority of expression was in the antisense orientation and from proviruses integrated within introns. We found two instances of long terminal repeat (LTR)-driven provirus transcription but no evidence to suggest that these active 5′ LTRs were influencing nearby host gene expression. Importantly, LTR-driven transcription was restricted to tumorigenic cells, suggesting that LTR promoter activity is dependent upon the transcriptional environment of a malignant cell.IMPORTANCEHere, we use anin vitromodel of human mammary epithelial cell transformation to assess how malignancy-associated shifts in the transcriptional milieu of a cell may impact HML-2 activity. We found 15 proviruses to be significantly expressed through four different mechanisms, with the majority of transcripts being antisense copies of proviruses located within introns. We saw active 5′ LTR use in tumorigenic cells only, suggesting that the cellular environment of a cancer cell is a critical component for induction of LTR promoter activity. These findings have implications for future studies investigating HML-2 as a target for immunotherapy or as a biomarker for disease.

scholarly journals Gene Expression Profile in Response to Doxorubicin–Rapamycin Combined Treatment of HER-2–Overexpressing Human Mammary Epithelial Cell Lines

2011 ◽  
Vol 11 (2) ◽  
pp. 464-474 ◽  
Author(s):  
Adriana Priscila Trapé ◽  
Maria Lucia Hirata Katayama ◽  
Rosimeire Aparecida Roela ◽  
Helena Brentani ◽  
Graziela Rosa Ravacci ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cell ◽  
Gene Expression Profile ◽  
Mammary Epithelial Cell ◽  
Combined Treatment ◽  
Human Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Epithelial Cell Lines ◽  
Human Mammary Epithelial ◽  
Her 2

scholarly journals Improved proteome coverage by using high efficiency cysteinyl peptide enrichment: The human mammary epithelial cell proteome

PROTEOMICS ◽  
2005 ◽  
Vol 5 (5) ◽  
pp. 1263-1273 ◽  
Author(s):  
Tao Liu ◽  
Wei-Jun Qian ◽  
Wan-Nan U. Chen ◽  
Jon M. Jacobs ◽  
Ronald J. Moore ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
High Efficiency ◽  
Human Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Proteome Coverage ◽  
Human Mammary Epithelial ◽  
Peptide Enrichment ◽  
Cell Proteome
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