A Division-Dependent Compartmental Model for Computing Cell Numbers in CFSE-based Lymphocyte Proliferation Assays

2012 ◽  
Author(s):  
H. T. Banks ◽  
W. C. Thompson ◽  
Cristina Peligero ◽  
Sandra Giest ◽  
Jordi Argilaguet ◽  
...  
Keyword(s):  
Lymphocyte Proliferation ◽  
Compartmental Model ◽  
Cell Numbers ◽  
Proliferation Assays

Enhancement of lymphocyte proliferation assays by use of serum-free medium

1992 ◽  
Vol 147 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Eric P. Kaldjian ◽  
Gwo-Hsiao Chen ◽  
Kemp B. Cease
Keyword(s):  
Lymphocyte Proliferation ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Proliferation Assays

Standardisation and quality assurance of lymphocyte proliferation assays for use in the assessment of immune function

1999 ◽  
Vol 227 (1-2) ◽  
pp. 85-97 ◽  
Author(s):  
K.S. Froebel ◽  
N.G. Pakker ◽  
F. Aiuti ◽  
M. Bofill ◽  
H. Choremi-Papadopoulou ◽  
...  
Keyword(s):  
Quality Assurance ◽  
Immune Function ◽  
Lymphocyte Proliferation ◽  
Proliferation Assays

Comparison of proliferative and immunomodulatory potential of adipose-derived mesenchymal stem cells from young and geriatric cats

2016 ◽  
Vol 19 (10) ◽  
pp. 1096-1102 ◽  
Author(s):  
Lara B Zajic ◽  
Tracy L Webb ◽  
Polly Webb ◽  
Jonathan W Coy ◽  
Steve W Dow ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
T Cell Activation ◽  
Lymphocyte Proliferation ◽  
Cell Activation ◽  
Diphenyltetrazolium Bromide ◽  
Significant Difference ◽  
Initial Culture ◽  
Autologous Therapy ◽  
Proliferation Assays

Objectives The objective of this study was to compare the ability of adipose-derived mesenchymal stem cells (aMSCs) generated from young vs geriatric cats to proliferate in culture, suppress lymphocyte proliferation and undergo senescence. Methods Adipose tissues from five young (<5 years) and six geriatric (>10 years) cats were harvested and cryopreserved for subsequent aMSC isolation and culture. aMSC proliferation in culture was compared via determination of time until passage two and by 3-(4,5-demethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The immunomodulatory capacity of aMSCs was assessed using lymphocyte proliferation assays, and senescence was evaluated using senescence-associated B-galactosidase (SABG) expression. All assays were performed on aMSCs between passage two and passage three. Results aMSCs from geriatric cats took significantly longer ( P = 0.008) to reach passage two (median 11 days, range 9–22 days) compared with aMSCs from young healthy cats (median 7 days, range 6–8 days). No significant difference was detected between young and geriatric cats in terms of their ability to suppress lymphocyte proliferation. SABG expression was not significantly different between young and geriatric aMSCs. Conclusions and relevance Compared with young feline aMSCs, geriatric aMSCs are significantly impaired in their ability to rapidly proliferate to passage two following initial culture, presenting a concern for autologous therapy. Nonetheless, once the cells are expanded, young and geriatric cat aMSCs appear to be equivalent in terms of their ability to functionally suppress T-cell activation and proliferation.

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