Comparison of MTT Colorimetric Assay and Tritiated Thymidine Uptake for Lymphocyte Proliferation Assays Using Chicken Splenocytes

1992 ◽  
Vol 36 (4) ◽  
pp. 1022 ◽  
Author(s):  
Denise I. Bounous ◽  
Raymond P. Campagnoli ◽  
John Brown
Keyword(s):  
Lymphocyte Proliferation ◽  
Colorimetric Assay ◽  
Tritiated Thymidine ◽  
Thymidine Uptake ◽  
Mtt Colorimetric Assay ◽  
Tritiated Thymidine Uptake ◽  
Proliferation Assays

Permeability alterations in amphibian embryos caused by salt solution and measured by tritiated thymidine uptake

Development ◽  
1964 ◽  
Vol 12 (3) ◽  
pp. 407-424
Author(s):  
Clarence A. Loeffler ◽  
Malcolm C. Johnston
Keyword(s):  
Salt Solution ◽  
Tap Water ◽  
Tritiated Thymidine ◽  
Permeability Barrier ◽  
Thymidine Uptake ◽  
Surface Coat ◽  
Amphibian Embryo ◽  
Amphibian Embryos ◽  
Tritiated Thymidine Uptake ◽  
Permeability Alterations

In attempts to label the nuclei of intact amphibian embryos with tritiated thymidine we found, in agreement with other authors (Tencer, 1961; Quertier, 1962; Chibon, 1962), that when the nucleoside is dissolved in tap water or media of low salt concentration it fails to enter the embryo. However, when tritiated thymidine was either injected into the embryo or applied to isolated tissues immersed in salt solution, the marker diffused readily through the tissues. Evidently the outer surface of the amphibian embryo is furnished with a special permeability barrier which prevents or reduces the entrance of these molecules. This barrier corresponds most likely to what Holtfreter (1943a, 1943b) has described as the ‘surface coat’. In order that the results of our present experiments may be better understood some of the pertinent properties of the coat will be reviewed. A protective coat, while still absent in the ovarian egg, arises after the egg is deposited in water.

Damage by Dichlorvos of Human Lymphocyte DNA

1980 ◽  
Vol 66 (4) ◽  
pp. 425-430 ◽  
Author(s):  
Paolo Perocco ◽  
Angela Fini
Keyword(s):  
Dna Synthesis ◽  
Human Lymphocyte ◽  
Tritiated Thymidine ◽  
Human Lymphocytes ◽  
Ultraviolet Rays ◽  
Thymidine Uptake ◽  
Short Term ◽  
In Vitro System ◽  
Tritiated Thymidine Uptake

The action of dichlorvos (2.2-dichlorovinyldimethyl phosphate) was studied with a short-term in vitro system which utilizes human lymphocytes. The parameters studied were the action exerted by the pesticide on scheduled (semiconservative) and unscheduled (reparative) DNA synthesis measured as tritiated thymidine uptake. The results obtained show that dichlorvos affects semiconservative DNA synthesis, damages human lymphocyte DNA inducing low reparative synthesis, and interferes with DNA repair processes after damage exerted by ultraviolet rays.

Vandellia Cordifolia Regulated Cell Proliferation and Cytokines Production in Human Mononuclear Cells

2000 ◽  
Vol 28 (03n04) ◽  
pp. 313-323 ◽  
Author(s):  
An-Pang Lin ◽  
Wei-Jern Tsai ◽  
Chi-Yen Fan ◽  
Ming-Jen Lee ◽  
Yuh-Chi Kuo
Keyword(s):  
Cell Cycle Progression ◽  
Mononuclear Cells ◽  
Tritiated Thymidine ◽  
Interleukin 2 ◽  
Interferon Γ ◽  
Thymidine Uptake ◽  
Dependent Manner ◽  
Human Mononuclear Cells ◽  
Tritiated Thymidine Uptake ◽  
Ifn Γ

Vandellia cordifolia (V. cordifolia) used for treatment inflammation in traditional Chinese medicine was selected for immunopharmacological activity test. The effects of V. cordifolia extracted fractions on human mononuclear cells (HMNC) proliferation were determined by tritiated thymidine uptake. The results indicated that VC-ME fraction suppressed HMNC proliferation activated with phytohemagglutinin (PHA) and stimulated cell cycle progression was arrested at the G0/G1 stage. The inhibitory mechanisms may involve the blocking of interleukin-2 (IL-2) and interferon-γ (IFN-γ) production, since VC-ME suppressed IL-2 and IFN-γ production of HMNC in a dose-dependent manner. Therefore, it is suggested that immunomodulatory agents are contained in V. cordifolia.

Sign in / Sign up

Export Citation Format

Share Document