Identification and Characterization of MYC Regulatory Elements: Links to Prostate Cancer

2012 ◽  
Author(s):  
Nora Wasserman ◽  
Marcelo Nobrega
Keyword(s):  
Prostate Cancer ◽  
Regulatory Elements ◽  
Identification And Characterization

scholarly journals Identification and characterization of cis-regulatory elements for photoreceptor type-specific transcription in zebrafish

2019 ◽  
Author(s):  
Wei Fang ◽  
Yi Wen ◽  
Xiangyun Wei
Keyword(s):  
Core Promoter ◽  
Regulatory Elements ◽  
Specific Gene ◽  
Protein Coding ◽  
Core Promoters ◽  
Protein Coding Genes ◽  
The Core ◽  
Cell Type Specific ◽  
Identification And Characterization

AbstractTissue-specific or cell type-specific transcription of protein-coding genes is controlled by both trans-regulatory elements (TREs) and cis-regulatory elements (CREs). However, it is challenging to identify TREs and CREs, which are unknown for most genes. Here, we describe a protocol for identifying two types of transcription-activating CREs—core promoters and enhancers—of zebrafish photoreceptor type-specific genes. This protocol is composed of three phases: bioinformatic prediction, experimental validation, and characterization of the CREs. To better illustrate the principles and logic of this protocol, we exemplify it with the discovery of the core promoter and enhancer of the mpp5b apical polarity gene (also known as ponli), whose red, green, and blue (RGB) cone-specific transcription requires its enhancer, a member of the rainbow enhancer family. While exemplified with an RGB cone-specific gene, this protocol is general and can be used to identify the core promoters and enhancers of other protein-coding genes.

scholarly journals Chromatin accessibility is dynamically regulated across C. elegans development and ageing

2018 ◽  
Author(s):  
Jürgen Jänes ◽  
Yan Dong ◽  
Michael Schoof ◽  
Jacques Serizay ◽  
Alex Appert ◽  
...  
Keyword(s):  
Regulatory Mechanism ◽  
Regulatory Elements ◽  
Chromatin Accessibility ◽  
Protein Coding ◽  
C Elegans ◽  
Transcription Profiles ◽  
Physiological Processes ◽  
Global Identification ◽  
Identification And Characterization

AbstractAn essential step for understanding the transcriptional circuits that control development and physiology is the global identification and characterization of regulatory elements. Here we present the first map of regulatory elements across the development and ageing of an animal, identifying 42,245 elements accessible in at least one C. elegans stage. Based on nuclear transcription profiles, we define 15,714 protein-coding promoters and 19,231 putative enhancers, and find that both types of element can drive orientation-independent transcription. Additionally, hundreds of promoters produce transcripts antisense to protein coding genes, suggesting involvement in a widespread regulatory mechanism. We find that the accessibility of most elements is regulated during development and/or ageing and that patterns of accessibility change are linked to specific developmental or physiological processes. The map and characterization of regulatory elements across C. elegans life provides a platform for understanding how transcription controls development and ageing.

scholarly journals Identification and characterization of biomarkers and their functions for docetaxel‑resistant prostate cancer cells

2019 ◽  
Author(s):  
Leihong Deng ◽  
Xiaopeng Gu ◽  
Tao Zeng ◽  
Fanghua Xu ◽  
Zhifeng Dong ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Prostate Cancer Cells ◽  
Identification And Characterization

Identification and characterization of selective androgen receptor degraders (SARDs) for the treatment of enzalutamide unresponsive and/or resistant prostate cancer

2017 ◽  
Vol 16 (3) ◽  
pp. e1288-e1289
Author(s):  
R. Getzenberg ◽  
S. Ponnusamy ◽  
T. Thiyagarajan ◽  
D.-J. Hwang ◽  
Y. He ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Androgen Receptor ◽  
Identification And Characterization

scholarly journals Identification and Characterization of a Novel Androgen Receptor Coregulator ARA267-α in Prostate Cancer Cells

2001 ◽  
Vol 276 (44) ◽  
pp. 40417-40423 ◽  
Author(s):  
Xin Wang ◽  
Shuyuan Yeh ◽  
Guan Wu ◽  
Cheng-Lung Hsu ◽  
Liang Wang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Androgen Receptor ◽  
Cancer Cells ◽  
Prostate Cancer Cells ◽  
Identification And Characterization
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