scholarly journals Asexual Embryogenesis and Plant Regeneration from Male Catkins of Quercus

HortScience ◽  
1991 ◽  
Vol 26 (9) ◽  
pp. 1217-1218 ◽  
Author(s):  
V.M. Gingas
Keyword(s):  
Plant Regeneration ◽  
Growth Regulators ◽  
Embryogenic Callus ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
White Oak ◽  
Callus Proliferation ◽  
Quercus Bicolor ◽  
Swamp White Oak ◽  
Asexual Embryogenesis

Partially expanded male catkins of swamp white oak (Quercus bicolor Willd.) and red oak (Quercus rubra L.) were cultured on Murashige and Skoog (MS) medium supplemented with BA or 2,4-D. Explants on 2,4-D produced a yellow embryogenic callus originating from the junction of the pedicel and peduncle. Subsequent transfers to MS with BA and then MS without growth regulators resulted in callus proliferation. After 10 to 14 weeks in culture, white embryoids developed from the callus of Q. bicolor. Separated and individually cultured embryoids underwent direct, repetitive embryogenesis. Upon transfer to l/2-strength MS, embryoid germination and plant regeneration occurred. Callus of Q. rubra degenerated after 5 months in culture, failing to yield embryogenic structures. Chemical names used: dichlorophenoxyacetic acid (2,4-D); benzyladenine (BA).

scholarly journals ASEXUAL EMBRYOGENESIS AND PLANT REGENERATION FROM MALE CATKINS OF QUERCUS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1101a-1101
Author(s):  
V.M. Gingas
Keyword(s):  
Plant Regeneration ◽  
Growth Regulators ◽  
Embryogenic Callus ◽  
Quercus Rubra ◽  
Ms Medium ◽  
Callus Proliferation ◽  
Quercus Bicolor ◽  
Asexual Embryogenesis ◽  
Repetitive Embryogenesis

Partially expanded male catkins at the pre-pollen shedding stage of Quercus rubra L. and Quercus bicolor Willd. were cultured on MS medium supplemented with BA or 2,4D Explants on 2,4D produced a yellow embryogenic callus, seeming to originate from the pedicels. Subsequent transfers to BA and then, MS without growth regulators, resulted in callus proliferation. After ten weeks in culture, white embryoids developed from the callus of Q. bicolor. Separated and individually cultured embryoids underwent direct, repetitive embryogenesis. Upon transfer to ½-strength MS, embryoid germination and plant regeneration occurred, Callus of Q. rubra degenerated after five months in culture, failing to produce embryogenic structures.

Comparing the Missouri Gravel Bed and a Wood Chip Production Method for Tree Growth

2019 ◽  
Vol 45 (6) ◽  
Author(s):  
Kelby Fite ◽  
Liza Holmes ◽  
Elden LeBrun
Keyword(s):  
Wood Chip ◽  
Production Method ◽  
White Oak ◽  
Gravel Bed ◽  
System A ◽  
Nursery Production ◽  
Quercus Bicolor ◽  
Root Stock ◽  
Swamp White Oak ◽  
Bare Root

Tree root defects from current nursery production practices influence short- and long-term tree performance and survivability. The Missouri Gravel Bed (MGB) system, a production method using gravel as a substrate, has been used to prevent many of these defects from occurring. MGB production involves planting bare root stock into a bed of gravel with frequent drip irrigation in order to produce a root system with relatively few defects. MGB production methods have also been purported to allow for summer transplanting of many species, as opposed to traditional dormant transplanting.Because gravel has low water- and nutrient-holding capacity, biochar (5% by volume) was incorporated into one plot as a possible means of improving both water- and nutrient-holding capacity over gravel alone. Wood chip mulch was also investigated as a growing substrate in place of the gravel in a growing system. In 2015, three species, Quercus bicolor (swamp white oak), Taxodium distichum (baldcypress), and Tilia cordata (littleleaf linden), were studied in pea gravel (PG), biochar-amended pea gravel (BC), and wood chip mulch bed (MB) growing environments. Very few differences occurred over the growing season with above- or belowground parameters indicating that the minimal-to-no-cost, more readily available substrate of wood chip mulch should be considered in these growing systems.

scholarly journals Callus induction and plant regeneration in the moss Aloina Aloides (Schultz) Kindb. (Pottiaceae, Bryopsida)

2003 ◽  
Vol 55 (3-4) ◽  
pp. 77-80 ◽  
Author(s):  
Aneta Bijelovic ◽  
Marko Sabovljevic
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Basal Medium ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Air Humidity ◽  
Moss Species ◽  
Bud Formation ◽  
Long Period ◽  
2,4 Dichlorophenoxyacetic Acid

Callus induction of moss species Aloina aloides (Schultz) Kindb. was obtained on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) or with 1.0 mg/L 2,4-D and 1.0 mg/L kinetin (KIN) or with 0.2 mg/L indole-3-butyric acid (IBA) and 2.0 mg/L 6-benzylaminopurine (BAP) or with 7.5 g/L of sucrose or with 15 g/L of sucrose or hormone - free and sugar free MS basal medium. The callus can be maintained for a long period of time without bud formation subcultured on the above media, at 16 h day/8 h night, 25 ? 2?C, 60-70% air humidity and irradiance of 50 ?mol m-2s-1. To obtain plant regeneration pieces, calli were transferred onto MS media supplemented with different concentrations of auxins and cytokinins (1.0 mg/L 2,4-D and 2 mg/L KIN; 0.2 mg/L IBA and 2 mg/L KIN; or 0.2 mg/L IAA and 2 mg/L BAP). In these media after subculturing, callus enlarges and turns to gametophytes with buds. Except for a smaller size, the plants obtained on the callus did not differ morphoanatomically from the shoots in the nature.

scholarly journals In vitro callus induction of gerbera from leaf explant

2020 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Sadia Afrin Jui ◽  
Md. Mijanur Rahman Rajib ◽  
M. Mofazzal Hossain ◽  
Sharmila Rani Mallik ◽  
Iffat Jahan Nur ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Leaf Explants ◽  
Leaf Explant ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Indole 3 Acetic Acid

The experiment was designed to evaluate the effect of growth regulators on leaf explant of Gerbera for callus induction. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Indole-3-acetic acid (IAA) were used to initiate cultures. These were added to Murashige and Skoog medium in different combinations and concentrations. Leaf explants cultured on MS medium supplemented with BAP+ 2, 4-D+ IAA in T4 treatment & BAP+ 2,4-D in T5 treatment showed the best results for callus induction. On the other hand callus was induced early in the combination of BA+ 2,4-D + IAA hormone in T5, T9 & T8 treatment respectively. The rate of callus induction was very low in BA + NAA combinations but it was much earlier.   

Early responses of sapwood of Quercus bicolor to mechanical injury

1970 ◽  
Vol 48 (4) ◽  
pp. 683-686 ◽  
Author(s):  
J. F. Wardell ◽  
J. H. Hart
Keyword(s):  
Mechanical Injury ◽  
Annual Rings ◽  
Triphenyl Tetrazolium Chloride ◽  
White Oak ◽  
Tetrazolium Chloride ◽  
Starch Grains ◽  
Quercus Bicolor ◽  
Swamp White Oak ◽  
Ray Parenchyma Cells ◽  
Extraneous Material

The response of sapwood of swamp white oak (Quercus bicolor Willd.) to mechanical injury was studied over a 28-day period. In cells within 0.5 cm of a wound stimulus, starch grains disappeared and cells were unable to reduce the vital stain, triphenyl tetrazolium chloride (TTC), 8 to 10 days after mechanical injury. Extraneous material and tannins began to accumulate 10 to 12 days after mechanical injury. Nuclei began to disintegrate 12 to 14 days after mechanical injury, but some persisted throughout the period of investigation.With increasing distance from the cambium, starch grains disappeared from ray parenchyma. Cells in the annual rings of sapwood adjacent to the heartwood were unable to reduce TTC. Nuclei were present in all annual rings of sapwood but were lacking in heartwood. Tannins appeared at the heartwood boundary and accumulated in the heartwood.With respect to the criteria used, this investigation indicates that formation of discoloration in sapwood and conversion of sapwood to heartwood appear similar.

scholarly journals Haploid plantlet regeneration through anther culture in oilseed Brassica species

1970 ◽  
Vol 34 (4) ◽  
pp. 693-703 ◽  
Author(s):  
MA Alam ◽  
MA Haque ◽  
MR Hossain ◽  
SC Sarker ◽  
R Afroz
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Growth Regulators ◽  
Callus Induction ◽  
Brassica Species ◽  
Plantlet Regeneration ◽  
Root Induction ◽  
Ms Medium ◽  
Half Strength

Anther of five varieties of Brassica species, namely BARI Shariaha-7, Tori-7, Agrani, Daulat and Safal were cultured in vitro to observe their regeneration potentiality. Different concentrations and combinations of growth regulators were supplemented in MS medium. The range of callus induction was 12.50-87.50 %. Maximum callus induction (75.00%) was observed on MS +4 mg/L 2, 4-D + 1.0 mg/L BAP. Among the genotypes, BARI Sharisha-7 showed the highest percentage of callus induction (60.42%). Among the treatments, highest percentage of shoot regeneration (75.00%) was observed on MS + 4 mg/L BAP + 1.0 mg/L NAA. BARI Sharisha-7 also showed the highest rate of plant regeneration (66.67%). Root induction was highest (75%) on half strength MS medium supplemented with 1.0 mg/L IBA and 0.5 mg/L NAA. The plantlets with sufficient roots thus obtained were transferred successfully to plastic pots and subsequently to the field. BARI Sharisha-7 and Tori-7 survived easily in the pots as well as in the field but Safal was very poor in survivability both in the pots and in the field. Key Words: Brassica; haploid; anther culture; in vitro regeneration.DOI: 10.3329/bjar.v34i4.5844Bangladesh J. Agril. Res. 34(4) : 693-703, December 2009 

EFFICIENT CALLUS INDUCTION AND PLANT REGENERATION VIA SOMATIC EMBRYOGENESIS FROM IMMATURE LEAF-DERIVED PROTOPLASTS OF GROUNDNUT (ARACHIS HYPOGAEA L.)

1996 ◽  
Vol 44 (4) ◽  
pp. 387-396 ◽  
Author(s):  
Perumal Venkatachalam ◽  
Narayanasamypillai Jayabalan
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Arachis Hypogaea ◽  
Callus Cultures ◽  
Alternative Methods ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
High Yields ◽  
2,4 Dichlorophenoxyacetic Acid

High yields of protoplasts were obtained from immature leaves of aseptically grown plants of Arachis hypogaea using an enzyme solution containing cellulase 2.0% (w/v) and Macerozyme 1.0% (w/v) in 0.6 M mannitol. Isolated protoplasts were cultured in Kao's medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). The protoplasts started to divide after 3–5 days of culture. Sustained divisions resulted in mass production of cell colonies and mini calli in 4 weeks. After 4 weeks, protoplast colonies were transferred to the Murashige and Skoog (MS) medium supplemented with a-naphthalene acetic acid (NAA) and BAP. Colonies proliferated into actively growing calli. Further attempts to regenerate plants from such calli were not successful. However, protoclones differentiated roots on the same medium. Alternative methods for plant regeneration from protoplast derived callus cultures were tried through somatic embryogenesis. Protoplast-derived calli treated with 2,4-D and BAP formed somatic embryos. Somatic embryogenesis began in the proembryo stage and proceeded from globular to dicotyledonary stage. Embryos were then transferred onto hormone-free MS medium for germination. Five to ten percent of these embryoids germinated and grew to plantlets. Regenerated plants were transferred to plastic cups and grown to maturity.

scholarly journals Suspension Culture and Plant Regeneration of Typha latifolia

HortScience ◽  
2002 ◽  
Vol 37 (2) ◽  
pp. 406-408 ◽  
Author(s):  
Lunique Estime ◽  
Marie O'Shea ◽  
Michael Borst ◽  
Jennifer Gerrity ◽  
Shih-Long Liao
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Line ◽  
Growth Regulators ◽  
Growth Curve ◽  
Typha Latifolia ◽  
Ms Medium ◽  
Growing Cell ◽  
Basal Media ◽  
Methoxybenzoic Acid

Typha latifolia L. (broadleaf cattail) callus was initiated from leaf sections, as well as from pistillate and staminate spikes. Two basal media in combination with three growth regulator regimes were tested for their capacity to induce callus from the explants. Pistillate spikes maintained in the dark on B5 medium supplemented with 5 mg·L-1 dicamba and 1 mg·L-1 BA produced the fastest growing cell line compared to other explants and media combinations. A growth curve in suspension culture was generated for this cell line on B5 medium. The mass of the callus increased by 150% by the end of the growth curve. Upon transfer of the callus to MS medium without growth regulators but with 3% sucrose and 3% phytagel, plants could be regenerated from 22% of the cultures. Chemical names used: 3,6-dichloro-2-methoxybenzoic acid (dicamba); N6-benzyladenine (BA).

scholarly journals PERTUMBUHAN KALUS DAUN MELON (Cucumis melo) VARIETAS MAI 119 DENGAN PEMBERIAN BAP (BENZYL AMINO PURINE) DAN 2,4-D (2,4 DICHLOROPHENOXYACETIC ACID)

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Nining Intan Toharah ◽  
Dwi Soelistya Dyah Jekti ◽  
Lalu Zulkifli
Keyword(s):  
Growth Regulators ◽  
Callus Induction ◽  
Cucumis Melo ◽  
Callus Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Benzyl Amino Purine

This study aims to determine the concentration of growth regulators BAP and   2,4-D which have the highest effect in stimulating the formation of callus melon plants (Cucumis melo) Mai 119 variety. Completely randomized design (CRD) was used in this research. Media used on callus induction was MS medium with addition of several concentration of BAP  (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) and 2,4-D (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) either alone or in a combination of both. Parameters measured were the time appearing of callus, callus diameter, callus texture, and callus color. Anova followed by Tukey's test was used to the analyse of time appearing of callus. Data of callus diameter was analyzed using Kruskal Wallis test followed by Mann-Whitney test. In the analysis of parameter related to the callus texture and callus color, descriptive test were used. The results showed that there were differences in the effect of growth regulators on the callus formation. The fastest callus induction and the largest diameter of callus were obtained on media with concentration of 2 mg/L BAP and 3 mg/L BAP.Keywords: BAP (benzyl amino purine), 2,4-D (2,4-dichlorophenoxyacetic acid), callus induction, melon (Cucumis melo) varieties Mai 119

QUERCUS BICOLOR, SWAMP WHITE OAK

2014 ◽  
pp. 1307-1308
Author(s):  
Henry John Elwes ◽  
Augustine Henry
Keyword(s):  
White Oak ◽  
Quercus Bicolor ◽  
Swamp White Oak
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