scholarly journals PERTUMBUHAN KALUS DAUN MELON (Cucumis melo) VARIETAS MAI 119 DENGAN PEMBERIAN BAP (BENZYL AMINO PURINE) DAN 2,4-D (2,4 DICHLOROPHENOXYACETIC ACID)

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Nining Intan Toharah ◽  
Dwi Soelistya Dyah Jekti ◽  
Lalu Zulkifli
Keyword(s):  
Growth Regulators ◽  
Callus Induction ◽  
Cucumis Melo ◽  
Callus Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Benzyl Amino Purine

This study aims to determine the concentration of growth regulators BAP and   2,4-D which have the highest effect in stimulating the formation of callus melon plants (Cucumis melo) Mai 119 variety. Completely randomized design (CRD) was used in this research. Media used on callus induction was MS medium with addition of several concentration of BAP  (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) and 2,4-D (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) either alone or in a combination of both. Parameters measured were the time appearing of callus, callus diameter, callus texture, and callus color. Anova followed by Tukey's test was used to the analyse of time appearing of callus. Data of callus diameter was analyzed using Kruskal Wallis test followed by Mann-Whitney test. In the analysis of parameter related to the callus texture and callus color, descriptive test were used. The results showed that there were differences in the effect of growth regulators on the callus formation. The fastest callus induction and the largest diameter of callus were obtained on media with concentration of 2 mg/L BAP and 3 mg/L BAP.Keywords: BAP (benzyl amino purine), 2,4-D (2,4-dichlorophenoxyacetic acid), callus induction, melon (Cucumis melo) varieties Mai 119

scholarly journals INDUKSI KALUS CENGKEH DARI EKSPAN DAUN MENGGUNAKAN 2,4-D SECARA IN VITRO

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Yulianti Rasud ◽  
Zainuddin Basri ◽  
Nirwan Sahiri
Keyword(s):  
Growth Regulators ◽  
Callus Induction ◽  
Callus Formation ◽  
Cell Mass ◽  
Medium Composition ◽  
Ms Medium ◽  
Plant Parts ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRACT Callus induction is one method of tissue culture which is done by stimulating cell division continuously from certain plant parts such as leaves, roots, stems, and so on by using growth regulators to form cell mass. The cell mass (callus) will then regenerate through organogenesis or embryogenesis to become a new plant. One of the growth regulators used for callus induction is 2,4-D. The aims of this experiments was to evaluate the best concentration of 2,4-D for callus induction of clove leaves. The experiment used Completely Randomized Design with treatment tested was concentrations of 2,4-D, consisted of six levels, namely 0.5 ppm, 1.0 ppm, 1.5 ppm, 2.0 ppm, 2.5 ppm and 3.0 ppm. Results of this experiments indicated that the best medium composition for callus induction was MS medium supplemented with 0.5 ppm 2,4-D.  In the medium composition, the fastest callus formation, namely 6.00 weeks after culture and the percentage of callus formation reached 100% with the color and texture of the resulting callus white and crumb. Keyword : Callus Induction, Clove, 2,4-DABSTRAK Induksi kalus merupakan salah satu metode kultur jaringan yang dilakukan dengan jalan memacu pembelahan sel secara terus menerus dari bagian tanaman tertentu seperti daun, akar, batang, dan sebagainya dengan menggunakan zat pengatur tumbuh hingga terbentuk massa sel. Massa sel (kalus) tersebut selanjutnya akan beregenerasi melalui organogenesis ataupun embriogenesis hingga menjadi tanaman baru. Salah satu zat pengatur tumbuh yang digunakan untuk induksi kalus adalah 2,4-D. Penelitian ini bertujuan menentukan konsentrasi 2,4-D yang lebih baik untuk induksi kalus daun cengkeh.  Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan tiga kali ulangan. Media dasar yang digunakan adalah media MS yang ditambahkan berbagai konsentrasi 2,4-D yaitu 0,50 ppm, 1,5 ppm, 2 ppm, 2,5 ppm, dan 3 ppm. Hasil penelitian menunjukkan bahwa komposisi media yang terbaik untuk induksi kalus daun cengkeh adalah media MS yang ditambahkan 0,5 ppm 2,4-D.  Pada komposisi media tersebut diperoleh saat muncul kalus paling cepat, yaitu rata-rata 6,00 MST dengan persentase pembentukan kalus tertinggi mencapai 100% dengan warna dan tekstur kalus yang dihasilkan putih dan remah. Kata Kunci :  Induksi Kalus, Cengkeh, 2,4-D.

scholarly journals Callus Formation on Endosperm from Immature and Mature Fruits of Barringtonia Racemosa

2019 ◽  
Vol 7 (4.14) ◽  
pp. 107
Author(s):  
D S M Soder ◽  
D N A A Khalid ◽  
A Saleh ◽  
F Pardi ◽  
N J Sidik
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Chemical Compounds ◽  
Dichlorophenoxyacetic Acid ◽  
Maturity Stage ◽  
Ms Medium ◽  
Fresh Weight ◽  
Pharmacological Activities ◽  
Friable Callus ◽  
2,4 Dichlorophenoxyacetic Acid

Barringtonia racemosa is mangroves type of plant which had been extensively utilized in conventional practices for relieving ailments of pain and inflammation. Many studies have been done on ethnobotanical profiles, pharmacological activities and chemical compounds in Barringtonia racemosa. However, there is a limited study on callogenesis of this plant particularly from different maturity stage of fruits. The present study is to identify the callogenesis of Barringtonia racemosa from endosperm explants of immature and mature fruits in MS medium supplemented with different concentrations of hormones 2,4-Dichlorophenoxyacetic acid (2,4-D) (0, 0.5, 1.0, 1.5 and 2.0 mg/L) and Kinetin (KIN) (0, 0.5, 1.0, 1.5 and 2.0 mg/L). The optimum hormone combination was found in callus grown on endosperm of immature fruits in MS medium supplemented with 1.5 mg/L 2,4-D and 1.0 mg/L KIN. It was also found that the callus in this treatment grew profusely with highest fresh weight (0.513 ± 0.022 g), 100% callus induction and friable callus texture. The callus fresh weight on endosperm explants was higher in immature fruits compared to mature fruits for all the hormone combinations. Therefore, callogenesis were found more efficient from endosperm explant of immature fruits in Barringtonia racemosa species.   

scholarly journals Callus Derived Regeneration of Some Selected Brassica Genotypes

2018 ◽  
Vol 15 (2) ◽  
pp. 1-10
Author(s):  
S K Biswas ◽  
M Z Tareq ◽  
S Ahmmed ◽  
A B M Z Hoque ◽  
M T Rahman
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Brassica Species ◽  
Direct Organogenesis ◽  
Stem Explants ◽  
Root Induction ◽  
Ms Medium ◽  
Cotyledon Explants ◽  
Randomized Design ◽  
Completely Randomized Design

The experiment was conducted to observe the callus induction ability of Brassica species. Plantlets were regenerated from cotyledon and stem explants of Brassica napus, Brassica campestris and Brassica juncea through direct organogenesis. The experiments were conducted in a Completely Randomized Design (CRD) with 4 replications. The highest frequency of callus formation was recorded in MS containing 2.0 mgl-1 BAP, 0.5 mgl-1 NAA and 2.0 mgl-1 AgNO3 in both stem and cotyledon explants. Among these explants, stem was found to be better responsive in callus induction than cotyledon. Among the genotypes used, BINA Sarisha-4 induced the highest percentage (100.00%) of callus from stem explants which was followed by BINA Sarisha-5 (100.00%) and Sampad (83.35%). On the other hand, BINA Sarisha-4 induced callus from 91.67% cotyledon explants, followed by BINA Sarisha-5 (75.00%) and Sampad (66.67%). Similarly, the highest percentage of shoot regeneration (58.34%) from stem explants of BINA Sarisha-4 was observed in MS medium supplemented with combination of hormone and silver nitrate concentrations. The highest percentage of root induction was 66.67 and 58.33% in plantlets derived from stem and cotyledon explants, respectively in ½ MS medium supplemented with 2.0 mgl-1 IBA and 0.5 mgl-1 of NAA. The highest survival rate was found after acclimatization of plants derived from stem (77.78%) and cotyledon (64.28%) explants of BINA Sarisha-4 in pot and 64.33 and 55.55%, respectively in field.The Agriculturists 2017; 15(2) 01-10

scholarly journals Callus Induction of Aerides odorata Lour. by Adding 2,4 Dichlorophenoxyacetic Acid (2,4-D)

2019 ◽  
Vol 7 (2) ◽  
pp. 109
Author(s):  
Azharia Khalida ◽  
Suwirmen Suwirmen ◽  
Zozy Aneloi Noli
Keyword(s):  
Plant Physiology ◽  
Tissue Culture ◽  
Natural Science ◽  
Callus Induction ◽  
Dichlorophenoxyacetic Acid ◽  
Fresh Weight ◽  
Greenish Yellow ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid

The research about callus induction of Aerides odorata L. by adding 2,4 Dichlorophenoxyacetic Acid (2,4-D), has been done from August to October 2018 in Plant Physiology and Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, Padang. The aim of this research was found the effective consentration of 2,4-D to induce somatic embryo of A.odorata. The research used Completely Randomized Design (CRD) with 5 treatments and 6 replications. The treatments were: 0 mg/L 2,4-D; 0,25 mg/L 2,4-D; 0,5 mg/L 2,4-D; 0,75 mg/L 2,4-D; 1 mg/L 2,4-D. The result showed that the treatmeant were able induction callus of A.odorata, with compact until the friable texture, color of the resulting callus is yellowish green and greenish yellow. 2,4-D 1 mg/L was the best concentration to increase fresh weight of callus.

scholarly journals Pengaruh Hormon Indole Butyric Acid (IBA) dan 6-Benzyl Amino Purin (BAP) terhadap Induksi Kalus Piper betle L. var Nigra

2019 ◽  
Vol 4 (2) ◽  
pp. 85-90
Author(s):  
Junairiah . ◽  
Artifa Rachmah ◽  
Yosephine Sri Wulan Manuhara ◽  
Ni’matuzahroh . ◽  
Lilis Sulistyorini ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Callus Formation ◽  
Dry Weight ◽  
Piper Betle ◽  
Fresh Weight ◽  
Indole Butyric Acid ◽  
Randomized Design ◽  
Benzyl Amino Purine

ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh kombinasi konsentrasi zat pengatur tumbuh IBA (Indole Butyric Acid) dan BAP (6-Benzyl Amino Purine) yang paling baik untuk induksi kalus sirih hitam (Piper betle L.). Penelitian ini menggunakan rancangan acak lengkap dengan 25 perlakuan dan setiap perlakuan memiliki 6 ulangan sehingga terdapat 150 unit ekperimen. Pada tahap kultur kalus dilakukan dengan menambahkan zat pengatur tumbuh IBA dan BAP ke dalam medium Murashige and Skoog (MS). Hasil uji tersebut menunjukkan bahwa zat pengatur tumbuh IBA dan BAP dengan kombinasi konsentrasi berbeda berpengaruh terhadap waktu induksi kalus, berat segar dan berat kering kalus sirih hitam. Hasil penelitian menunjukkan waktu tercepat pembentukan kalus pada IBA 2,0 mg/L dan BAP 2,0 mg/L yaitu 10 hari. Berat segar dan berat kering tertinggi pada IBA 2,0 mg/L dan 2,0 mg/L yaitu 0,8507 gram untuk berat segar dan 0,0769 untuk berat kering. Warna kalus adalah putih kehijauan dengan tekstur kompak dan remah.Kata kunci: Induksi kalus, Piper betle L., Indole Butyric Acid, 6-Benzyl Amino Purine.ABSTRACTThe purpose of this research to determine the effect of the combination concentration of growth regulators IBA (Indole Butyric Acid) and BAP (6-Benzyl Amino Purine) was best for callus induction black betel (Piper betle L.). This research used completely randomized design with 25 treatments and 6 replicates of each treatment, hence there were 150 experimental units. At this stage of callus culture was done by adding the growth regulators IBA and BAP into Murashige and Skoog (MS). The test results showed that plant growth regulators IBA and BAP in combination with different concentrations of influence on callus induction time, fresh weight and dry weight callus Piper betle L. The results showed the fastest time of callus formation at IBA 2,0 mg/L and BAP 2,0 mg/L at 10 days. Fresh weight and dry weight of the highest in the IBA 2,0 mg/L and BAP 2,0 mg/L were 0,8507 grams and 0,0769 grams fresh weight to dry weight. The color of callus was white greenish with compact and friable texture.Keywords: Callus induction, Piper betle L., Indole Butyric Acid, 6-Benzyl Amino Purine.

scholarly journals Pertumbuhan Kalus Rejasa (Elaeocarpus grandiflorus) dari Eksplan Tangkai Daun pada Kondisi Gelap

Life Science ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Nur Wijawati ◽  
Noor Aini Habibah ◽  
Fajar Musafa ◽  
Khoirul Mukhtar ◽  
Y. Ulung Anggraito ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Growth Regulators ◽  
Callus Induction ◽  
Callus Formation ◽  
Growth Time ◽  
Dichlorophenoxyacetic Acid ◽  
Independent Variables ◽  
Callus Regeneration ◽  
Dark Conditions ◽  
2,4 Dichlorophenoxyacetic Acid

Rejasa (Elaeocarpus grandiflorus) is a Salatiga identity plant which is now rarely found. Rejasa produces secondary metabolites that have the potential as drugs. This study tested the growth of rejasa callus in the medium with the addition of various types and concentrations of growth regulators. The independent variables used were the type and concentration of growth regulators (2,4-dichlorophenoxyacetic acid (2,4-D) with concentrations of 1.5; 2.5 and 3.5 ppm and Picloram with a concentration of 3.5; 5; and 7.5 ppm). The dependent variable in this study was the growth of callus regeneration (percentage of callus growth, time of callus formation and morphology of callus) observed for five months in dark conditions. Explants used were young petiolus and the medium used in this study was medium Murashige and Skoog (MS). The results showed the lowest percentage of callus induction was found in explants with the addition of Picloram growth regulators with a concentration of 7.5 ppm (14%). Explants maintained in the medium with the addition of Picloram with a concentration of 5 ppm resulted in the highest percentage of callus induction. The time of callus induction is in the range of 10-22 days. The explants with the addition of Picloram growth regulator substances with a concentration of 5 ppm had the best callus induction time, which was 12 days. Most of the callus formed was friable and yellowish. Based on the results of this research, the best medium for callus induction of rejasa in dark conditions was medium with the addition of 5 ppm Picloram.   Rejasa (Elaeocarpus grandiflorus) adalah tanaman identitas Salatiga yang mulai jarang ditemukan. Pertumbuhan populasinya memiliki perkembangan yang lambat. Perkembangan generatif melalui perkecambahan biji terjadi dalam tingkat yang sangat rendah. Dalam kelangkaannya, rejasa memiliki khasiat sebagai tanaman obat melalui metabolit sekunder yang dihasilkan. Penelitian ini menguji pertumbuhan kalus rejasa dalam variasi jenis dan konsentrasi zat pengatur tumbuh. Variabel bebas yang digunakan adalah jenis dan konsentrasi zat pengatur tumbuh (2,4-dichlorophenoxyacetic acid (2,4-D) dengan konsentrasi 1,5; 2,5; dan 3,5 ppm serta pikloram dengan konsentrasi 3,5; 5; dan 7,5 ppm). Variabel terikat dalam penelitian ini adalah pertumbuhan kalus rejasa (persentase tumbuh kalus, waktu berkalus, dan morfologi kalus) dalam medium yang diamati selama 5 bulan. Eksplan yang digunakan adalah tangkai muda yang ditanam dalam medium agar Murashige & Skoog dengan penambahan zat pengatur tumbuh 2,4-D dan pikloram dalam berbagai konsentrasi dan dipelihara dalam kondisi gelap. Hasil penelitian menunjukkan persentase induksi kalus paling rendah terdapat pada eksplan dengan penambahan zat pengatur tumbuh pikloram dengan konsentrasi 7,5 ppm (14%). Eksplan yang dipelihara pada medium dengan penambahan pikloram konsentrasi 5 ppm memghasilkan persentasi induksi kalus tertinggi. Waktu induksi kalus berada dalam rentang 10-22 hari. Eksplan dengan penambahan zat pengatur tumbuh pikloram konsentrasi 5 ppm memiliki rerata waktu induksi kalus paling baik yaitu 12 hari. Kalus yang terbentuk dominan berwarna kekuningan dengan jenis meremah. Berdasarkan hasil penelitiaan, medium yang paling baik untuk induksi kalus dalam kondisi gelap adalah medium MS dengan penambahan zat pengatur tumbuh pikloram konsentrasi 5 ppm.

scholarly journals Callus induction and plant regeneration in the moss Aloina Aloides (Schultz) Kindb. (Pottiaceae, Bryopsida)

2003 ◽  
Vol 55 (3-4) ◽  
pp. 77-80 ◽  
Author(s):  
Aneta Bijelovic ◽  
Marko Sabovljevic
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Basal Medium ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Air Humidity ◽  
Moss Species ◽  
Bud Formation ◽  
Long Period ◽  
2,4 Dichlorophenoxyacetic Acid

Callus induction of moss species Aloina aloides (Schultz) Kindb. was obtained on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) or with 1.0 mg/L 2,4-D and 1.0 mg/L kinetin (KIN) or with 0.2 mg/L indole-3-butyric acid (IBA) and 2.0 mg/L 6-benzylaminopurine (BAP) or with 7.5 g/L of sucrose or with 15 g/L of sucrose or hormone - free and sugar free MS basal medium. The callus can be maintained for a long period of time without bud formation subcultured on the above media, at 16 h day/8 h night, 25 ? 2?C, 60-70% air humidity and irradiance of 50 ?mol m-2s-1. To obtain plant regeneration pieces, calli were transferred onto MS media supplemented with different concentrations of auxins and cytokinins (1.0 mg/L 2,4-D and 2 mg/L KIN; 0.2 mg/L IBA and 2 mg/L KIN; or 0.2 mg/L IAA and 2 mg/L BAP). In these media after subculturing, callus enlarges and turns to gametophytes with buds. Except for a smaller size, the plants obtained on the callus did not differ morphoanatomically from the shoots in the nature.

scholarly journals Embryogenic Callus Induction of Aquilaira Malaccensis Lam. and Aquilaria Subintegra Ding Hou

2019 ◽  
Vol 9 (1) ◽  
pp. 5746-5751
Keyword(s):  
Callus Induction ◽  
Embryogenic Callus ◽  
Callus Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Aseptic Culture ◽  
Aquilaria Malaccensis ◽  
The Family ◽  
Commercially Important ◽  
Embryogenic Callus Induction ◽  
2,4 Dichlorophenoxyacetic Acid

Aquilaria malaccensis Lam. and Aquilaria subintegra Ding Hou belong to the family of Thymelaeaceae which is commonly known as gaharu or agarwood. It is a commercially important tree and identified as a potential aromatic plant. The overwhelming responses in the lodging sector reduce gaharu species in the forest. Mass propagation through plant tissue culture technology will substitute this problem. The present study was conducted to investigate the embryogenic callus induction between these two species. The most optimum sterilization method for both species was sodium hypochlorite 5.0% which gave the highest percentage of aseptic culture (95%) with the absence of tissue browning. The leaves of both species were cultured on Murashige and Skoog, (1962) (MS) media supplemented with combination of various concentrations of 6-benzylaminopurine (BAP) (0.5, 1.0, 2.0 and 2.5 mg/L) and 2,4-dichlorophenoxyacetic acid (2, 4-D) (0.5, 1.0, 1.5 and 2.0 mg/L) and kept under dark condition. The explants produced embryogenic, white and compact callus at the end cut of the explants after two weeks of culture in all treatments. The highest frequency of embryogenic callus formation was observed in explants cultured on 2.0 mg/L BAP and 0.5 mg/L 2,4-D for both species. From the present study, the optimum sterilization technique and embryogenic callus induction for A. malaccensis Lam. and A. subintegra were established.

scholarly journals In vitro callus induction of gerbera from leaf explant

2020 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Sadia Afrin Jui ◽  
Md. Mijanur Rahman Rajib ◽  
M. Mofazzal Hossain ◽  
Sharmila Rani Mallik ◽  
Iffat Jahan Nur ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Leaf Explants ◽  
Leaf Explant ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Indole 3 Acetic Acid

The experiment was designed to evaluate the effect of growth regulators on leaf explant of Gerbera for callus induction. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Indole-3-acetic acid (IAA) were used to initiate cultures. These were added to Murashige and Skoog medium in different combinations and concentrations. Leaf explants cultured on MS medium supplemented with BAP+ 2, 4-D+ IAA in T4 treatment & BAP+ 2,4-D in T5 treatment showed the best results for callus induction. On the other hand callus was induced early in the combination of BA+ 2,4-D + IAA hormone in T5, T9 & T8 treatment respectively. The rate of callus induction was very low in BA + NAA combinations but it was much earlier.   

scholarly journals Viability of Cyperus rotundus L. tubers after application of herbicide in pre- and postemergence

2020 ◽  
Vol 87 ◽  
Author(s):  
Ana Ligia Giraldeli ◽  
André Felipe Moreira Silva ◽  
Felipe Carrara de Brito ◽  
Robson Josadaque Nogueira de Lima ◽  
Beatriz Sizilio dos Santos ◽  
...  
Keyword(s):  
Active Ingredient ◽  
Dry Matter ◽  
Visual Control ◽  
Cyperus Rotundus ◽  
Dichlorophenoxyacetic Acid ◽  
Purple Nutsedge ◽  
Chemical Management ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid

ABSTRACT Chemical management is the most widely adopted technique to control weeds in sugarcane crops. Purple nutsedge (Cyperus rotundus L.) is among the most difficult species to control. Herbicides need to be absorbed and translocated in a sufficient amount for the tuber chain to be effective. Thus, this study aimed to evaluate the effectiveness of the herbicides sulfentrazone, diclosulam, imazapic, imazapyr, halosulfuron, ethoxysulfuron, monosodium methyl arsenate (MSMA), and 2,4-dichlorophenoxyacetic acid (2,4-D) in controlling C. rotundus and reducing the viability of its tubers. The experiment was carried out in a greenhouse in a completely randomized design, with nine treatments and four replications. The herbicides sulfentrazone (800 g active ingredient – ai·ha?1), diclosulam (193.17 g ai·ha?1), imazapic (133 g ai·ha?1), and imazapyr (500 g acid equivalent – ae·ha?1) were applied in preemergence at five days after planting the tubers, while halosulfuron (112.5 g ai·ha?1), ethoxysulfuron (135 g ai·ha?1), MSMA (1,975 g ai·ha?1), and 2,4-D (1,340 g ae·ha?1) were applied in postemergence (4 to 5 leaves). The parameters visual control, shoot dry matter, number of tubers and bulbs, weight of tubers + bulbs, number of epigeal manifestations, and tuber viability were analyzed. The herbicides diclosulam, halosulfuron, and ethoxysulfuron provided 100% control of the shoot at 90 days after application (DAA). All herbicides reduced the number of bulbs, weight of tubers + bulbs, and shoot dry matter. The herbicides sulfentrazone, imazapic, halosulfuron, ethoxysulfuron, and MSMA provided the highest reduction in tuber viability.

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