scholarly journals Suspension Culture and Plant Regeneration of Typha latifolia

HortScience ◽  
2002 ◽  
Vol 37 (2) ◽  
pp. 406-408 ◽  
Author(s):  
Lunique Estime ◽  
Marie O'Shea ◽  
Michael Borst ◽  
Jennifer Gerrity ◽  
Shih-Long Liao
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Line ◽  
Growth Regulators ◽  
Growth Curve ◽  
Typha Latifolia ◽  
Ms Medium ◽  
Growing Cell ◽  
Basal Media ◽  
Methoxybenzoic Acid

Typha latifolia L. (broadleaf cattail) callus was initiated from leaf sections, as well as from pistillate and staminate spikes. Two basal media in combination with three growth regulator regimes were tested for their capacity to induce callus from the explants. Pistillate spikes maintained in the dark on B5 medium supplemented with 5 mg·L-1 dicamba and 1 mg·L-1 BA produced the fastest growing cell line compared to other explants and media combinations. A growth curve in suspension culture was generated for this cell line on B5 medium. The mass of the callus increased by 150% by the end of the growth curve. Upon transfer of the callus to MS medium without growth regulators but with 3% sucrose and 3% phytagel, plants could be regenerated from 22% of the cultures. Chemical names used: 3,6-dichloro-2-methoxybenzoic acid (dicamba); N6-benzyladenine (BA).

scholarly journals Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra

2008 ◽  
Vol 43 (10) ◽  
pp. 1325-1330 ◽  
Author(s):  
Lucymeire Souza Morais-Lino ◽  
Janay Almeida dos Santos-Serejo ◽  
Sebastião de Oliveira e Silva ◽  
José Raniere Ferreira de Santana ◽  
Adilson Kenji Kobayashi
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Somatic Embryos ◽  
Culture Media ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Male Flowers

The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerate plants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 µM of IAA and 2.2 µM of BAP.

scholarly journals Haploid plantlet regeneration through anther culture in oilseed Brassica species

1970 ◽  
Vol 34 (4) ◽  
pp. 693-703 ◽  
Author(s):  
MA Alam ◽  
MA Haque ◽  
MR Hossain ◽  
SC Sarker ◽  
R Afroz
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Growth Regulators ◽  
Callus Induction ◽  
Brassica Species ◽  
Plantlet Regeneration ◽  
Root Induction ◽  
Ms Medium ◽  
Half Strength

Anther of five varieties of Brassica species, namely BARI Shariaha-7, Tori-7, Agrani, Daulat and Safal were cultured in vitro to observe their regeneration potentiality. Different concentrations and combinations of growth regulators were supplemented in MS medium. The range of callus induction was 12.50-87.50 %. Maximum callus induction (75.00%) was observed on MS +4 mg/L 2, 4-D + 1.0 mg/L BAP. Among the genotypes, BARI Sharisha-7 showed the highest percentage of callus induction (60.42%). Among the treatments, highest percentage of shoot regeneration (75.00%) was observed on MS + 4 mg/L BAP + 1.0 mg/L NAA. BARI Sharisha-7 also showed the highest rate of plant regeneration (66.67%). Root induction was highest (75%) on half strength MS medium supplemented with 1.0 mg/L IBA and 0.5 mg/L NAA. The plantlets with sufficient roots thus obtained were transferred successfully to plastic pots and subsequently to the field. BARI Sharisha-7 and Tori-7 survived easily in the pots as well as in the field but Safal was very poor in survivability both in the pots and in the field. Key Words: Brassica; haploid; anther culture; in vitro regeneration.DOI: 10.3329/bjar.v34i4.5844Bangladesh J. Agril. Res. 34(4) : 693-703, December 2009 

scholarly journals ASEXUAL EMBRYOGENESIS AND PLANT REGENERATION FROM MALE CATKINS OF QUERCUS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1101a-1101
Author(s):  
V.M. Gingas
Keyword(s):  
Plant Regeneration ◽  
Growth Regulators ◽  
Embryogenic Callus ◽  
Quercus Rubra ◽  
Ms Medium ◽  
Callus Proliferation ◽  
Quercus Bicolor ◽  
Asexual Embryogenesis ◽  
Repetitive Embryogenesis

Partially expanded male catkins at the pre-pollen shedding stage of Quercus rubra L. and Quercus bicolor Willd. were cultured on MS medium supplemented with BA or 2,4D Explants on 2,4D produced a yellow embryogenic callus, seeming to originate from the pedicels. Subsequent transfers to BA and then, MS without growth regulators, resulted in callus proliferation. After ten weeks in culture, white embryoids developed from the callus of Q. bicolor. Separated and individually cultured embryoids underwent direct, repetitive embryogenesis. Upon transfer to ½-strength MS, embryoid germination and plant regeneration occurred, Callus of Q. rubra degenerated after five months in culture, failing to produce embryogenic structures.

scholarly journals Potential Embryogenic Callus Induction Protocol Through Cell Suspension Culture For High Frequency Plant Regeneration Of Maspine Pineapple (Ananas comosus L.)

1970 ◽  
Vol 3 (2) ◽  
pp. 40-45
Author(s):  
M.F. Mohamad Bukhori ◽  
Norzulaani Khalid ◽  
Ch'ng Lou Ven
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Ananas Comosus ◽  
Ms Medium ◽  
Embryogenic Callus Induction

To explore the potential for embryogenic callus induction protocol through cell suspension culture forhigh frequency plant regeneration of Maspine pineapple (Ananas comosus L.), eight different culturemedia formulation were evaluated for their effects on the induction of somatic embryos from suckerexplants. Explants were cultured on MS medium supplemented with various media concentration(NAA, Dicamba and BAP, Picloram, Kinetin and NAA, 2,4-D, TDZ, and TDZ and BAP).Embryogenic callus induction percentage, color and texture of the callus were assessed after fivemonths of culture. The optimum medium for the proliferation of in vitro shoots from sucker explantswas MS medium supplemented with 3 mg/L BAP. Meanwhile, the optimum medium for the inductionof fastest and high percentage of embryogenic callus growth from in vitro leaf-based was MS mediumsupplemented with Picloram. Results of mean comparison showed that 3 mg/L Picloram were moreeffective on explants than 10 mg/L. Results of the double staining method proved that somaticembryogenesis occurred in MS supplemented with 3 mg/L Picloram. Under microscopic observations,the globular-stage of the embryos were revealed in callus cells which is relatively suitable forsuspension cells inoculums, indicating that the tested PGR were significantly effective for somaticembryogenesis formation in this species. Most embryogenic callus from sucker explants wasyellowish-mucilaginous-wet-friable. The developed protocol potentially leads to the production ofembryogenic callus from sucker explants and plant regeneration through somatic embryogenesis.

scholarly journals In vitro regeneration potentiality of Brassica genotypes in differential growth regulators

1970 ◽  
Vol 35 (2) ◽  
pp. 189-199 ◽  
Author(s):  
MMA Khan ◽  
ABM Arif Hasan Khan Robin ◽  
MAN Nazim-Ud-Dowla ◽  
SK Talukder ◽  
L Hassan
Keyword(s):  
Plant Regeneration ◽  
Growth Regulators ◽  
Callus Induction ◽  
Root Formation ◽  
Ms Medium ◽  
Differential Growth ◽  
The Media ◽  
Regenerated Plantlets

Petiole of six genotypes of oilseed Brassica viz. Tori-7, Sampad, Kallyania, BARI Sarisha-7, BARI Sarisha-8, and MM 20-3 were cultured in MS medium with different concentrations of BAP, NAA, and AgNO3 for callus induction and subsequent plant regeneration. The highest percentage of callus induction (91.43%) was observed in Tori-7 in the media supplemented with 2 mg/L BAP, 0.1 mg/L NAA and 2.0 mg/L AgNO3. Calli were maintained in order to get sufficient number of regenerants. With the increased concentration of BAP, the highest percentage (57.14) of regenerants were found in Tori-7 followed by Sampad (33.13%) and BARI Sarisha-8 (31.42%) in MS media supplemented with 2.5 mg/L BAP, 0.1 mg/L NAA and 2.0 mg/L AgNO3. Root formation from the regenerants was found best in half MS medium supplemented with 0.5 mg/L NAA in genotype Tori-7. Regenerated plantlets of four genotypes (Tori-7, BARI Sarisha-8, Kallyania, BARI Sarisha-7) were successfully established in the field.Keywords: AgNO3; BAP; Brassica; NAA; regeneration.  DOI: 10.3329/bjar.v35i2.5881Bangladesh J. Agril. Res. 35(2) : 189-199, June 2010

scholarly journals In vitro propagation of banana

1970 ◽  
Vol 34 (2) ◽  
pp. 269-278
Author(s):  
M Rezaul Karim ◽  
MA Malek ◽  
Sajia Rahman ◽  
M Al-Amin ◽  
M Ruhul Amin
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Room Temperature ◽  
Ms Medium ◽  
Musa Sp ◽  
In Vitro Technique ◽  
Half Strength ◽  
The Mean ◽  
Basal Media

An in vitro technique for plant regeneration using meristem-derived plantlets of banana cv. BARI-l (Musa sp.) has been developed. Highest number of shoot regeneration was noticed on basal media supplemented with 7.5 mgL-1 BAP + 0.5 mgL-1 NAA at 30 days after inoculation (DAI). The mean number of shoots significantly reduced when the concentrations of BAP and NAA in the medium was high. Regenerated shoots were rooted on half strength MS medium containing 0.5 mgL-1 IAA + 0.5 mgL-1 IBA at 30 DAI. In vitro raised plantlets were transferred to poly bags containing ground soil and cowdung mixture (1:1) for acclimatization and hardening in room temperature (28-30°C) and the established plantlets are ready for planting in the field. Key Words: In vitro propagation; banana; Musa sp.DOI: 10.3329/bjar.v34i2.5799Bangladesh J. Agril. Res. 34(2): 269-278, June 2009

Somatic Embryogenesis and Plant Regeneration of Wiregrass (Aristida stricta) and Creeping Bluestem (Schizachyrium scoparium var. stoloniferum)

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 479a-479
Author(s):  
Wusi Chen ◽  
Jeffrey G. Norcini ◽  
Robert S. Kalmbacher ◽  
James H. Aldrich
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Growth Regulators ◽  
Cell Suspension Culture ◽  
Embryogenic Callus ◽  
Basal Medium ◽  
Suspension Cultures ◽  
Embryoid Formation ◽  
Semi Solid

Initiation of callus and induction of embryogenesis were achieved from both wiregrass and creeping bluestem. MS basal medium containing coconut milk, sucrose, and 2,4-D were used to initiate callus from young inflorescence of wiregrass and creeping bluestem. The presence of 2,4-D was found to be essential for the induction and early development of embryoids, possibly up to the globular stage. In the case of bluestem, initiation of embryogenic callus required the presence of a low concentration of BA; using only 2,4-D resulted in more non-embryogenic callus. More globular embryos were formed when embryogenic cultures grew rapidly without subculturing, or after being transferred to a hormone-free or a reduced 2,4-D medium. Plant regeneration was carried on a hormone-free MS medium. Initiation of cell suspension and induction of embryoid formation of wiregrass were achieved. However, maintaining cell suspensions seems to have some problems. A majority of the cells were thick-walled, elongated, and non-dividing. No embryos were formed in suspension cultures planted onto solid media. Reinitiation of cell suspension culture of wiregrass is in progress. Initiation of creeping bluestem cell suspension culture was carried out in MS basal medium containing coconut milk, sucrose, and 2,4-D. The maintenance of the cell suspension cultures and induction of embryoid formation were tested under different combinations and concentrations of growth regulators. Suspension cultures were selected and planted onto semi-solid MS basal medium with or without growth regulators. Somatic embryoids formed from suspension culture 3 to 4 weeks after being planted on semi-solid medium. Germination and plant regeneration of somatic embryoid of creeping bluestem are in progress.

scholarly journals Asexual Embryogenesis and Plant Regeneration from Male Catkins of Quercus

HortScience ◽  
1991 ◽  
Vol 26 (9) ◽  
pp. 1217-1218 ◽  
Author(s):  
V.M. Gingas
Keyword(s):  
Plant Regeneration ◽  
Growth Regulators ◽  
Embryogenic Callus ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
White Oak ◽  
Callus Proliferation ◽  
Quercus Bicolor ◽  
Swamp White Oak ◽  
Asexual Embryogenesis

Partially expanded male catkins of swamp white oak (Quercus bicolor Willd.) and red oak (Quercus rubra L.) were cultured on Murashige and Skoog (MS) medium supplemented with BA or 2,4-D. Explants on 2,4-D produced a yellow embryogenic callus originating from the junction of the pedicel and peduncle. Subsequent transfers to MS with BA and then MS without growth regulators resulted in callus proliferation. After 10 to 14 weeks in culture, white embryoids developed from the callus of Q. bicolor. Separated and individually cultured embryoids underwent direct, repetitive embryogenesis. Upon transfer to l/2-strength MS, embryoid germination and plant regeneration occurred. Callus of Q. rubra degenerated after 5 months in culture, failing to yield embryogenic structures. Chemical names used: dichlorophenoxyacetic acid (2,4-D); benzyladenine (BA).

scholarly journals Somatic embryogenesis and plant regeneration from cell suspension cultures of Gentiana kurroo Royle.

2018 ◽  
Vol 7 (5) ◽  
pp. 2239
Author(s):  
Shiwani Kaushal ◽  
Arushdeep Sidana
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Cell Suspension ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Western Himalayas ◽  
Ms Medium ◽  
Gentiana Kurroo

Gentiana kurroo Royle, native to North- Western Himalayas, is one of the critically endangered perennial herbs, which is being overexploited due to its multiple medicinal uses. The goal of this work was to develop a protocol for somatic embryogenesis and efficient plant regeneration through cell suspension culture. Selected ranges of plant growth regulators were experimented for somatic embryogenesis. Light micrographs of the established suspension cell cultures of somatic embryos at different developmental stages were also observed under light microscope. Somatic embryogenesis was induced from the leaf explants and the maximum induction frequency (97.2%) was obtained on Murashige and Skoog (MS) medium supplemented with 0.5 mg/l each of NAA, BAP and TDZ. Cell suspension cultures were established in MS liquid medium containing IAA (0.5 mg/l) + BAP (1.0 mg/l) with 89-93% viable cells. Transfer of cotyledonary somatic embryos to the MS agar medium containing 2, 4-D (0.4 mg/l) and KN (1.5 mg/l) resulted in somatic embryogenesis at high frequencies (80%) with an average of 28 ± 0.2 embryos/ callus piece. After transfer onto the half strength MS medium without growth regulators approximately 80-90% somatic embryos developed into complete plantlets. The protocol described here could be used for somatic hybridization, genetic transformation, isolation of protoplasts and large-scale propagation of G. kurroo.

scholarly journals Influence of growth regulators on plant regeneration in tomato

2011 ◽  
Vol 32 (No. 3) ◽  
pp. 118-122
Author(s):  
J. Gubiš ◽  
Z. Lajchová ◽  
L. Klčová ◽  
Z. Jureková
Keyword(s):  
Plant Regeneration ◽  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Ms Medium ◽  
Explant Type ◽  
Regeneration Capacity

We studied the effect of different plant growth regulators on in vitro regeneration and plant growth of three cultivars of tomato (Lycopersicon esculentum Mill.) from explants derived from hypocotyls and cotyledons of aseptically grown seedlings. The regeneration capacity was significantly influenced by cultivar and explant type. The highest number of shoots regenerated in both types of explants was recorded on MS medium supplemented with 1.0 mg/dm<sup>3</sup> zeatin and 0.1 mg/dm<sup>3</sup> IAA. The cultivar UC 82 showed the best regeneration capacity on all types of used media. The most responsive explants were hypocotyls with 90&ndash;92% regeneration in dependence on the used cultivars and mean production from 0.18 to 0.38 shoots per explant. &nbsp;

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