scholarly journals SHOOT REGENERATION FROM LEAF TISSUES OF AMERICAN ELM AND SIBERIAN ELM.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 584f-584 ◽  
Author(s):  
Z. M. Cheng ◽  
N. O. Shi ◽  
L. Tokach ◽  
B. K. Gaschk
Keyword(s):  
Factorial Design ◽  
Shoot Regeneration ◽  
Regeneration System ◽  
Ulmus Americana ◽  
Regeneration Rate ◽  
American Elm ◽  
Wide Range ◽  
Leaf Tissues

Shoot regeneration was obtained from leaf tissues of American (Ulmus americana) and Siberian elm (U. pumila) seedlings germinated in vitro and in greenhouse. Murashige and Skoog (MS, 1962) media supplemented with 4 levels of BA (0, 5, 10, 15, 20 μM) and 3 levels of IBA (0, 2.5, 5.0 μM) were tested in a factorial design to find an optimal hormonal combination for shoot regeneration. Shoot regeneration was obtained from both species within 3-4 weeks in a wide range of media. The highest regeneration rate (50%) of American elm was in the medium containing 10 μM BA and 2.5 μM IBA. Incubation under the light was essential for a higher rate of regeneration. Gelrite was found as a better solidifying agent than agar. The progress is under way to achieve transgenic elms by combining this regeneration system with Agrobacterium-mediated transformation.

scholarly journals Plant Regeneration of Periwinkle (Catharanthus roseus) via Organogenesis

HortScience ◽  
2008 ◽  
Vol 43 (3) ◽  
pp. 832-836 ◽  
Author(s):  
Andrea Swanberg ◽  
Wenhao Dai
Keyword(s):  
Plant Regeneration ◽  
Shoot Regeneration ◽  
Woody Plant ◽  
Naphthalene Acetic Acid ◽  
Regeneration System ◽  
Dark Treatment ◽  
Regeneration Rate ◽  
Shoot Production ◽  
Leaf Tissues

Two periwinkle cultivars, Pacific Coral (P1) and Sunstorm Rose (P2), were used for development of a plant regeneration system. Leaf and internodal explants collected from in vitro plants were plated onto woody plant medium (WPM) using a factorial arrangement of 6-benzyladeine (BA) and 1-naphthalene acetic acid (NAA). Shoots were successfully regenerated. Shoot production from leaf tissues was minimal for all cultivars, whereas internodal tissues showed variable rates of regeneration depending on the hormone combination. Cultivar P1 showed the maximum regeneration rate (73.3%) when internodal explants, 4 to 6 mm in length, were placed on WPM containing 5 μm BA and 5 μm NAA. Cultivar P2 showed a regeneration rate of 56.7% with a combination of 20 μm BA and 10 μm NAA. Shoot regeneration rate increased as the internodal explant size increased for P2; however, the regeneration rate decreased when the explant size was greater than 7 mm for P1. The shoot regeneration rate decreased as the period of the dark treatment of internodal explants increased in both P1 and P2. The antibiotics carbenicillin (Carb) and cefotaxime (Cef) had little effect on shoot regeneration. There was a slightly higher rate observed for P1 when Cef was added into the medium, whereas P2 showed a decrease with the addition of Cef. Carb showed no significant effect on shoot regeneration for both cultivars. Addition of both Carb and Cef to the medium slightly inhibited shoot regeneration.

scholarly journals In Vitro Shoot Regeneration of Georgia Plume, Elliottia racemosa, from Multiple Genotypes Collected from Wild Populations

HortScience ◽  
2011 ◽  
Vol 46 (2) ◽  
pp. 287-290
Author(s):  
Carrie A. Radcliffe ◽  
James M. Affolter ◽  
Hazel Y. Wetzstein
Keyword(s):  
Tissue Culture ◽  
Shoot Regeneration ◽  
Adventitious Shoots ◽  
Shoot Elongation ◽  
Shoot Cultures ◽  
Regeneration System ◽  
Low Genetic Diversity ◽  
Wide Range ◽  
In Vitro Shoot Regeneration

Georgia plume (Elliottia racemosa, Ericaceae) is a threatened, woody plant endemic to Georgia's Coastal Plain region in the southeastern United States. Populations of the plant have a fragmented distribution within a restricted range and are characterized by low genetic diversity and a lack of sexual recruitment. Georgia plume cannot be effectively propagated using conventional methods. We have developed an in vitro shoot regeneration system that is effective with explants obtained from mature plants in the wild. The objective of this study was to determine the efficacy of using this in vitro protocol to regenerate proliferating shoot cultures from 34 georgia plume genotypes obtained from divergent populations. Young expanding leaves were cultured on Gamborg's media supplemented with 10 μM thidiazuron and 5 μM indole-3-acetic acid. After 8 weeks, tissues were transferred to a shoot elongation medium with 25 μM 2-isopentenyl adenine. Of the 34 genotypes tested, 91% formed shoot primordia and 85% regenerated shoots within 6 months of inoculation. This study verifies that tissue culture can be used to produce adventitious shoots from a wide range of georgia plume genotypes. Within a coordinated conservation program, tissue culture is a feasible system to use for safeguarding and reintroduction of genetically diverse plant material, which may be critical to the survival of this rare species.

scholarly journals Factors Affecting Plant Regeneration from Leaf Tissues of Buddleia Species

HortScience ◽  
2007 ◽  
Vol 42 (7) ◽  
pp. 1670-1673 ◽  
Author(s):  
Wenhao Dai ◽  
Cielo Castillo
Keyword(s):  
Shoot Regeneration ◽  
Basal Medium ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Dark Treatment ◽  
Regeneration Rate ◽  
Factors Affecting ◽  
In Vitro Shoots ◽  
Leaf Tissues

The effects of genotype, basal medium, plant growth regulator (PGR), dark treatment, and antibiotics on shoot regeneration of two Buddleia cultivars, B. davidii ‘Potters Purple’ and Buddleia ‘Lochinch’, were investigated. In vitro shoots were regenerated from leaf tissues in either Murashige and Skoog (MS) or woody plant medium (WPM) media supplemented with benzyladenine (BA). In general, more shoots were regenerated in WPM medium than in MS medium. Dark treatment for 3 to 5 weeks dramatically increased shoot regeneration. Addition of indole-3-butyric acid (IBA) or naphthalene acetic acid (NAA) significantly enhanced the regeneration rate and shoots of each explant. The maximum regeneration rate (100%) of B. davidii ‘Potters Purple’ was achieved when cultured in WPM containing 5 μm BA plus 5 μm IBA. The maximum regeneration rate (98.4%) of Buddleia ‘Lochinch’ was found in WPM supplemented with 20 μm BA plus 4 μm IBA. Carbenicillin at 250 to 500 mg·L−1 and cefotaxime at 125 to 250 mg·L−1, individually or combined, promoted shoot regeneration. Interactions between genotype and medium or PGRs were found. In vitro shoots were easily rooted in half-strength MS medium with or without NAA. Rooted plants were transferred to potting mix and grown in the greenhouse. This research will facilitate genetic improvement and fast propagation of Buddleia species using biotechnology.

Establishment of an efficient shoot regeneration system in vitro in Brassica rapa

2021 ◽  
Author(s):  
Ying Zhao ◽  
Shengnan Huang ◽  
Yun Zhang ◽  
Fengyan Shi ◽  
Xuyao Liu ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Shoot Regeneration ◽  
Regeneration System

scholarly journals In vitro shoot regeneration of boldo from leaf explants

2010 ◽  
Vol 40 (10) ◽  
pp. 2210-2213
Author(s):  
Monalize Salete Mota ◽  
Juliana de Magalhães Bandeira ◽  
Eugenia Jacira Bolacel Braga ◽  
Valmor João Bianchi ◽  
José Antonio Peters
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Shoot Development ◽  
High Potential ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Bud Induction ◽  
Molecular Studies ◽  
In Vitro Shoot Regeneration

A shoot regeneration system for Plectranthus neochilus was studied from leaf explants. Leaves developed under in vitro conditions were cultured on Wood Plant Medium supplemented with 0.2mg dm-3 α-naphthaleneacetic acid (NAA) and different 6-benzilaminopurine (BAP) or thidiazuron (TDZ) concentrations (0, 1.5, 3.0, 4.5 and 6.0mg dm-3). An increase in percentage of responsive explants (85.3%) and in the number of shoots developed per explant (3.2) was observed when the explants were treated with 5.3 and 4.7mg dm-3 BAP, respectively. The leaf explants cultured on media supplemented with TDZ became vitreous and did not form buds. The regeneration system used is efficient for boldo bud induction and shoot development, showing high potential for advanced cellular and molecular studies.

In Vitro Regeneration of Valencia-type Peanut (Arachis hypogaea L.) from Cultured Petiolules, Epicotyl Sections and Other Seedling Explants1

1992 ◽  
Vol 19 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Ming Cheng ◽  
David C. H. Hsi ◽  
Gregory C. Phillips
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Arachis Hypogaea L ◽  
Wide Range ◽  
Cultivated Peanut

Abstract This study evaluated plant development via direct organogenesis from in vitro-cultured young seedling tissues of cultivated peanut, especially the valencia-type peanut. Complete plants were regenerated from in vitro-cultured petiolule-with-blade-attached explants, leaflet segments, and epicotyl andpetiole sections. Multiple shoots arose on Murashige and Skoog medium (MS) supplemented with 6-benzylaminopurine (BA) (5–25 mg/L) plus 1-naphthaleneacetic acid (NAA) (0.5–3 mg/L). After 30 d culture on 25 mg/L BA + 1 mg/L NAA, 1.6 buds or shoots/explant were regenerated from the petiolule-with-blade-attached explants. Comparable numbers of shoots were obtained from epicotyl sections of the first node region of the seedling after 60 d culture using 10 mg/L BA + 1 mg/L NAA. Leaflet segments and petiole sections were less responsive for shoot formation. Excised shoots developed roots in vitro upon transfer for 15 d to MS medium supplemented with NAA at 1 mg/L. Plantlets were transferred to soil and grown in a greenhouse to maturity. A wide range of cultivated peanut genotypes was evaluated for organogenic responsiveness, using the petiolule-with-blade-attached explant source. Only valencia-type cultivars, or a hybrid derivative with a Valencia background, were responsive with this regeneration system.

scholarly journals Preculture Medium Promotes Direct Shoot Regeneration from Micropropagated Strawberry Leaf Disks

HortScience ◽  
1993 ◽  
Vol 28 (1) ◽  
pp. 55-57 ◽  
Author(s):  
S. Sorvari ◽  
S. Ulvinen ◽  
T. Hietaranta ◽  
H. Hiirsalmi
Keyword(s):  
Shoot Regeneration ◽  
Casein Hydrolysate ◽  
Fragaria Ananassa ◽  
Direct Shoot Regeneration ◽  
Regeneration Medium ◽  
Indolebutyric Acid ◽  
Regeneration Rate ◽  
In Vitro Plantlets ◽  
Leaf Disks

The effect of preculturing in vitro plantlets of two strawberry (Fragaria ×ananassa Duch.) cultivars grown on micropropagation medium with and without hormones on regenerating shoots from leaf disks was examined. Preculturing stock plants on micropropagation medium with hormones (BAP at 0.5 mg·liter-1 + IBA at 0.5 mg·liter-1 GA, at 0.2 mg·liter-1) promoted shoot regeneration in the two cultivars tested. Using hormone-containing micropropagation medium for preculture, the highest mean regeneration rate of 9.9 shoots per total number of leaf disks was obtained for the Finnish cultivar Hiku on modified Murashige and Skoog (MS) regeneration medium supplemented with (in mg·liter-1) 2000 KNO3, 400 casein hydrolysate (CH), 3 BAP, and 0.1 IBA. For the Norwegian cultivar Jonsok, the highest mean regeneration rate of 12.8 shoots per total number of leaf disks was obtained on modified MS regeneration medium with (in mg·liter-1) 600 CH, 3 BAP, and 0.1 IBA. Chemical names used: 6-benzylaminopurine (BAP); 3-indolebutyric acid (IBA); gibberellic acid (GA).

Indirect shoot regeneration of Iranian purple coneflower (Echinacea purpurea L.) from cotyledon and hypocotyl explants

2011 ◽  
Vol 59 (1) ◽  
pp. 65-72 ◽  
Author(s):  
A. Zebarjadi ◽  
J. Motamedi ◽  
A. Ismaili
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Shoot Formation ◽  
Ms Medium ◽  
Medicinal Species ◽  
Regeneration Rate ◽  
Hypocotyl Explants ◽  
Purple Coneflower ◽  
Significant Difference

In vitro plant regeneration was optimized for Iranian purple coneflower via organogenesis from callus cultures derived from cotyledon and hypocotyl tissues by placing them on MS medium supplemented with different concentrations and combinations of BAP and NAA. The experiment was laid out as a completely randomized design in a factorial arrangement with three replications. The results indicated that the mean callus induction was influenced by explant type, with a significant difference between cotyledon (77.81%) and hypocotyl (65.33%) explants at the 0.01 probability level. In relation with the regeneration rate, no significant differences were observed between the two types of explants. For both cotyledons and hypocotyls the optimum shoot regeneration frequency (31.5% and 32.5%, respectively) and number of shoots per explant (5.2 and 5.3, respectively) were achieved using medium supplemented with 0.4 mg l−1 BAP. Proliferated shoots were elongated in hormone-free MS medium and well-developed shoots were rooted on MS medium, both with and without the addition of 2 mg l−1 IBA. All the plantlets survived acclimatization, producing normal plants under controlled conditions. This study revealed that cotyledon and hypocotyl explants of E. purpurea have relatively good potential for callus induction and shoot formation. Furthermore, a beneficial method has been established for the micropropagation of this valuable medicinal species.

In vitro conservation of American elm (Ulmus americana): potential role of auxin metabolism in sustained plant proliferation

2012 ◽  
Vol 42 (4) ◽  
pp. 686-697 ◽  
Author(s):  
Mukund R. Shukla ◽  
A. Maxwell P. Jones ◽  
J. Alan Sullivan ◽  
Chunzhao Liu ◽  
Susan Gosling ◽  
...  
Keyword(s):  
Dutch Elm Disease ◽  
Multiplication Rate ◽  
Mature Trees ◽  
Endangered Tree ◽  
In Vitro Proliferation ◽  
Ulmus Americana ◽  
Auxin Metabolism ◽  
American Elm

An efficient procedure for the conservation of mature American elm ( Ulmus americana L.) trees that have survived the epidemics of Dutch elm disease and are potential sources of disease resistance is reported. The model utilizes in vitro proliferation of fresh and dormant buds from mature trees for cloning nearly 100 year old American elm trees. The key factors that influenced sustained growth and multiplication included optimization of culture process and auxin metabolism in the source tissue. Blocking the action of endogenous auxins through the addition of antiauxin in the proliferation medium was crucial for high multiplication rate and optimum shoot development. Addition of antiauxin also mitigated the decline in productivity observed with multiple subcultures, which will enable long-term conservation of selected germplasm. The most effective medium for long-term proliferation contained 5.0 µmol/L p-chlorophenoxyisobutyric acid with 2.2 µmol/L benzylaminopurine and 0.29 µmol/L gibberellic acid. Medium with 2.5 µmol/L indole-3-butyric acid was the best for rooting microshoots (89%). Rooted plantlets were readily acclimatized to the greenhouse environment with a 90% survival rate. The strategy developed for American elm will aid in increasing multiplication of resistant clones, facilitate long-term conservation of elite genotypes, and also provide an approach to improve conservation of other endangered tree species.

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