scholarly journals RAPD Banding Patterns in Sweetpotato Infected with Fusarium lateritium

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 912C-912
Author(s):  
F.A. Buffone ◽  
D.R. LaBonte ◽  
C.A. Clark
Keyword(s):  
Environmental Conditions ◽  
Fusarium Species ◽  
Shoot Tips ◽  
Common Disease ◽  
Banding Patterns ◽  
Fusarium Lateritium ◽  
Bright Sunlight ◽  
Chlorotic Leaf ◽  
Pcr Techniques ◽  
Leaf Distortion

Chlorotic leaf distortion is a common disease of sweetpotato caused by Fusarium lateritium. This fungus is unique among Fusarium species in that it grows epiphytically on leaves and shoot tips of sweetpotato. Fusarium lateritium mycelia appear as white masses on leaves, and this fungus can cause chlorosis under periods of bright sunlight. When environmental conditions are not favorable for growth, this organism is not readily observed on sweetpotato. The objective of this research was to see if DNA of F. lateritium is amplified using PCR techniques during amplification of sweetpotato DNA. Our results show cTAB extracts of sweetpotato inoculated with F. lateritium have additional bands not present in a control free of F. lateritium. Furthermore, these bands correspond to banding patterns obtained from the F. lateritium isolate DNA when amplified alone. Researchers who use sweetpotato tissue in PCR-based research, e.g., phylogenetic research, should be aware of these amplified products. This situation is further compounded because numerous F. lateritium biotypes are present in the environment.

scholarly journals RAPD BANDING PATTERNS IN SWEETPOTATO INFECTED WITH FUSARIUM LATERITIUM

HortScience ◽  
1994 ◽  
Vol 29 (7) ◽  
pp. 728f-728
Author(s):  
Frank A. Buffone ◽  
Don R. La Bonte
Keyword(s):  
Common Disease ◽  
Banding Patterns ◽  
Fusarium Lateritium ◽  
Leaf Chlorosis ◽  
Cloudy Weather ◽  
Bright Sunlight ◽  
Chlorotic Leaf ◽  
Preliminary Study ◽  
Foreign Dna ◽  
Leaf Distortion

Chlorotic Leaf Distortion (CLD) is a common disease of sweetpotato caused by Fusarium lateritium. This fungus is unique among Fusarium species in that it grows on the epidermis of leaves and shoot tips of sweetpotato. Fusarium lateritium appears as a white epiphytic material and under bright sunlight causes leaf chlorosis. When cloudy weather persists for several days, all symptoms disappear. Researchers who use RAPD to examine banding patterns of sweetpotato DNA assume that foreign DNA present in the cTAB extract is quantitatively low and will not appreciably amplify and appear as bands. In this study we found the modified cTAB procedure used to amplify sweetpotato DNA also amplifies DNA of Fusarium lateritium cultures. DNA banding patterns of infected leaves was compared with those free of the disease. No differences in banding patterns were observed in this preliminary study.

scholarly journals FUSARIUM LATERITIUM CAUSES SWEETPOTATO CHLOROTIC LEAF DISTORTION.

HortScience ◽  
1990 ◽  
Vol 25 (8) ◽  
pp. 855b-855
Author(s):  
C. A. Clark ◽  
R. A. Valverde ◽  
J. A. Wilder-Ayers ◽  
P. E. Nelson
Keyword(s):  
Leaf Primordia ◽  
Apical Meristems ◽  
Fusarium Lateritium ◽  
Leaf Surfaces ◽  
Meristem Tip Culture ◽  
Chlorotic Leaf ◽  
Sunny Weather ◽  
Leaf Distortion ◽  
White Material ◽  
Fungal Mycelia

Symptoms of chlorotic leaf distortion (CLD) develop on vigorously growing sweetpotato (Ipomoea batatas) plants during sunny weather. They include chlorosis and twisting of young, expanding leaves and the appearance of white material on the adaxial leaf surfaces. The white material consisted of extramatrical fungal mycelia and Fusarium macroconidia. Fusarium lateritium Nees was isolated from surface-sterilized vine segments, leaf primordia, apical meristems, flower parts and true seeds of plants with CLD. Meristem-tip-culture-derived plants (mericlones) did not develop symptoms when grown for extended periods under disease-conducive conditions in the greenhouse. The fungus was not isolated from mericlones or other plants which had remained symptomless in the greenhouse but was isolated from lower nodes of symptomless plants from growers' fields. Symptoms developed on 84% of 185 mericlones of nine sweetpotato genotypes inoculated with F. lateritium isolated from CLD-affected plants. The pathogen was reisolated only from inoculated mericlones.

scholarly journals Heritability Estimates for Reaction Resistance to Sweetpotato Chlorotic Leaf Distortion Caused by Fusarium lateritium

HortScience ◽  
1996 ◽  
Vol 31 (3) ◽  
pp. 433-435
Author(s):  
Bong-kyoo Kim ◽  
Don R. La Bonte ◽  
Christopher A. Clark ◽  
Mario I. Buteler
Keyword(s):  
Ipomoea Batatas ◽  
Variance Component Analysis ◽  
Breeding Cycle ◽  
Individual Plant ◽  
Narrow Sense ◽  
Narrow Sense Heritability ◽  
Fusarium Lateritium ◽  
Chlorotic Leaf ◽  
Heritability Estimates ◽  
Leaf Distortion

Narrow-sense heritabilities for reaction to chlorotic leaf distortion (CLD), incited by Fusarium lateritium Nees: Fr., were estimated in sweetpotato [Ipomoea batatas (L.) Lam] by variance component analysis and parent–offspring regression. Visually rated severity reactions to CLD varied greatly among the 20 parents used to generate half-sib progeny from open-pollinated nurseries in 1990 and 1991. Progeny from each nursery were evaluated along with parents in a completely randomized design in two consecutive years. Narrow-sense heritability (h2) estimates based on variance components were moderate on an entry mean basis at 0.61 in 1990, 0.38 in 1991, and 0.33 for the two years combined. Slightly higher, but still moderate, estimates were obtained on an individual plant basis. Narrow-sense heritability estimates using parent–offspring regression were 0.35 in 1990, 0.33 in 1991, and 0.33 for the two years combined. Predicted next-generation response was highest using a half-sib family recurrent selection among three schemes compared at a 10% selection intensity. Our data indicate –0.63 improvement in the half-sib family CLD severity rating in one breeding cycle.

Studies on sweetpotato with chlorotic leaf distortion, caused byFusarium lateritium

1996 ◽  
Vol 42 (1) ◽  
pp. 67-70 ◽  
Author(s):  
Bong‐Kyoo Kim ◽  
Don R. La Bonte ◽  
Christopher A. Clark
Keyword(s):  
Chlorotic Leaf ◽  
Leaf Distortion

scholarly journals Trichothecene Nonproducer Gibberella Species Have Both Functional and Nonfunctional 3-O-Acetyltransferase Genes

Genetics ◽  
2003 ◽  
Vol 163 (2) ◽  
pp. 677-684
Author(s):  
Makoto Kimura ◽  
Takeshi Tokai ◽  
Gentaro Matsumoto ◽  
Makoto Fujimura ◽  
Hiroshi Hamamoto ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Fission Yeast ◽  
Fusarium Graminearum ◽  
Sequence Similarity ◽  
Fusarium Species ◽  
Fungal Genome ◽  
Expression Cloning ◽  
Nucleotide Sequence Similarity ◽  
Cdna Expression ◽  
Pcr Techniques

Abstract The trichothecene 3-O-acetyltransferase gene (FgTri101) required for trichothecene production by Fusarium graminearum is located between the phosphate permease gene (pho5) and the UTP-ammonia ligase gene (ura7). We have cloned and sequenced the pho5-to-ura7 regions from three trichothecene nonproducing Fusarium (i.e., F. oxysporum, F. moniliforme, and Fusarium species IFO 7772) that belong to the teleomorph genus Gibberella. BLASTX analysis of these sequences revealed portions of predicted polypeptides with high similarities to the TRI101 polypeptide. While FspTri101 (Fusarium species Tri101) coded for a functional 3-O-acetyltransferase, FoTri101 (F. oxysporum Tri101) and FmTri101 (F. moniliforme Tri101) were pseudogenes. Nevertheless, F. oxysporum and F. moniliforme were able to acetylate C-3 of trichothecenes, indicating that these nonproducers possess another as yet unidentified 3-O-acetyltransferase gene. By means of cDNA expression cloning using fission yeast, we isolated the responsible FoTri201 gene from F. oxysporum; on the basis of this sequence, FmTri201 has been cloned from F. moniliforme by PCR techniques. Both Tri201 showed only a limited level of nucleotide sequence similarity to FgTri101 and FspTri101. The existence of Tri101 in a trichothecene nonproducer suggests that this gene existed in the fungal genome before the divergence of producers from nonproducers in the evolution of Fusarium species.

Tomato chlorotic leaf distortion virus, a new bipartite begomovirus infecting Solanum lycopersicum and Capsicum chinense in Venezuela

2011 ◽  
Vol 156 (12) ◽  
pp. 2263-2266 ◽  
Author(s):  
Karla Zambrano ◽  
Francis Geraud-Pouey ◽  
Doris Chirinos ◽  
Gustavo Romay ◽  
Edgloris Marys
Keyword(s):  
Solanum Lycopersicum ◽  
Bipartite Begomovirus ◽  
Capsicum Chinense ◽  
Chlorotic Leaf ◽  
Leaf Distortion
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