SEEKING POTATO CLONES WITH ELEVATED TUBER METHIONINE USING PROTOPLAST REGENERATION PROTOCOLS
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`Russet Burbank' leaf protoplasts were cultured in cell layer (CL) media containing the amino acid analog, ethionine (ETN), at concentrations ranging from 0 to 9 μm. A decrease in plating efficiency was observed when ETN concentrations in CL exceeded 3 μm. Calli that developed were placed on C media containing ETN concentrations up to 200 μm. Regenerated plants were grown to maturity in a growth chamber and resulting tubers were analyzed for free methionine. Selected protoclones produced tubers whose free methionine content exceeded that of the `Russet Burbank' control by ≈3-fold. Methodology used will be discussed.
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1997 ◽
Vol 119
(28)
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pp. 6457-6460
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1983 ◽
Vol 192
(1-2)
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pp. 235-240
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1974 ◽
Vol 46
(1)
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pp. 255-270
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2008 ◽
Vol 95
(7)
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pp. 3306-3321
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1980 ◽
Vol 141
(1)
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pp. 100-105
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