Cucumis melo L. New Breeding Lines Tolerant to Melon Vine Decline

Two 24-mer primers, MUSKFOM I and MUSKFOM II, were developed that amplify a 1.5-kb DNA fragment in race 1 Fusarium wilt resistant muskmelon (Cucumis melo L.), but not in race 1 susceptible germplasm tested. Three race 1 resistant cultivars and two race 1 resistant breeding lines as well as eight race 1 susceptible lines were analyzed using the two sequence-specific primers in the polymerase chain reaction. These primers should prove valuable for nondestructive determination of Fom 2 gene introgression in breeding programs.

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Olpidium bornovanus-Mediated Germination of Ascospores of Monosporascus cannonballus: A Host-Specific Rhizosphere Interaction

Phytopathology ◽

10.1094/phyto-11-10-0313 ◽

2011 ◽

Vol 101 (7) ◽

pp. 794-796 ◽

Cited By ~ 7

Author(s):

Michael E. Stanghellini ◽

Iraj J. Misaghi

Keyword(s):

Cucumis Melo ◽

Soil Microflora ◽

Field Soil ◽

Population Densities ◽

Monosporascus Cannonballus ◽

Zoosporic Fungus ◽

Olpidium Bornovanus ◽

Ascospore Germination ◽

Vine Decline ◽

Cucumis Melo L

Monosporascus cannonballus, a host-specific root-infecting ascomycete, is the causal agent of a destructive disease of melon (Cucumis melo L.) known as vine decline. Ascospores germinate only in the rhizosphere of melon plants growing in field soil. However, no germination occurs in the rhizosphere of melon plants if the field soil is heated to temperatures >50°C prior to infestation with ascospores. This observation suggested that germination is mediated by one or more heat-sensitive members of the soil microflora. Although bacteria or actinomycetes were heretofore suspected as the germination-inducing microbes, our data demonstrate that Olpidium bornovanus, an obligate, host-specific, root-infecting zoosporic fungus, is responsible. In four experiments conducted in autoclaved field soil amended with various population densities of culturally produced ascospores, significant ascospore germination was recorded only in the rhizosphere of cantaloupe seedlings colonized by O. bornovanus.

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Assessment and Classification of Volatile Profiles in Melon Breeding Lines Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry

Plants ◽

10.3390/plants10102166 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2166

Author(s):

Drishti Majithia ◽

Rita Metrani ◽

Nitin Dhowlaghar ◽

Kevin M. Crosby ◽

Bhimanagouda S. Patil

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Cucumis Melo ◽

Solid Phase Microextraction ◽

Solid Phase ◽

Principal Component ◽

Gas Chromatography Mass Spectrometry ◽

Breeding Lines ◽

Chromatography Mass Spectrometry ◽

Cucumis Melo L

Cucumis melo L is one of the most commercial and economical crops in the world with several health beneficial compounds as such carotenoids, amino acids, vitamin A and C, minerals, and dietary fiber. Evaluation of the volatile organic compounds (VOCs) in different melon (Cucumis melo L.) breeding lines provides useful information for improving fruit flavor, aroma, and antimicrobial levels. In this study, the VOCs in 28 melon breeding lines harvested in 2019 were identified and characterized using head space solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). This identified 113 VOCs with significant differences in composition and contents of among the breeding lines, including 15 esters, 27 aldehydes, 35 alcohols, 14 ketones, 4 acids, 10 hydrocarbons, 5 sulfurs, and 3 other compounds. The highest average contents of all the VOCs were found in BL-30 (13,973.07 µg/kg FW) and the lowest were in BL-22 (3947.13 µg/kg FW). BL-9 had high levels of carotenoid-derived VOCs. The compounds with the highest contents were benzaldehyde, geranylacetone, and β-ionone. Quality parameters such as color and sugar contents of melons were also measured. All the melon color readings were within the typical acceptable range. BL-22 and BL-14 had the highest and lowest sugar contents, respectively. Principal component analysis (PCA) produced diverse clusters of breeding lines based on flavor and aroma. BL-4, BL-7, BL-12, BL-20, and BL-30 were thus selected as important breeding lines based on their organoleptic, antimicrobial, and health-beneficial properties.

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Differential Fruit Load in Melon (Cucumis melo L.) Affects Shoot and Root Growth, and Vine Decline Symptoms

HortScience ◽

10.21273/hortsci.32.3.526b ◽

1997 ◽

Vol 32 (3) ◽

pp. 526B-526 ◽

Cited By ~ 2

Author(s):

David W. Wolff ◽

Daniel I. Leskovar ◽

Mark C. Black ◽

Marvin E. Miller

Keyword(s):

Root Rot ◽

Cucumis Melo ◽

Growth Parameters ◽

Symptom Expression ◽

Germplasm Screening ◽

Fruit Maturity ◽

Vine Decline ◽

Cucumis Melo L ◽

Root Size ◽

Fruit Load

The effect of zero, one, and two fruits per vine on plant growth and reaction to Monosporascus root rot/vine decline were investigated. In the first study, four cultivars with differing levels of tolerance were evaluated (`Primo', `Deltex', `Caravelle', `Magnum 45'). Vine decline ratings were taken weekly during the harvest period for 4 weeks. Treatments with no fruit showed delayed and less-severe vine decline symptoms. Temperature also effected vine decline symptom expression. In a Fall test, with lower temperatures during fruit maturity, symptoms were delayed in all treatments and often absent in treatments with no fruit load. Vine decline symptom expression is greatly effected by physiological (fruit load) and temperature stress. A subsequent study was conducted to more precisely quantify the effect of various fruit loads on shoot/root partitioning and vine decline symptoms. In addition to growth parameters root disease ratings were taken. `Caravelle', the most-susceptible genotype, was grown under differing fruit loads as mentioned above in Weslaco and Uvalde, Texas. As fruit load increased, root size decreased. Increased vine decline symptoms were observed under higher fruit loads. The implications on germplasm screening and breeding for resistance will be discussed.

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