scholarly journals In Vitro Shoot Proliferation and Root Induction of Shoot Tip Explants from Mature Male Plants of Casuarina cunninghamiana Miq.

HortScience ◽  
2010 ◽  
Vol 45 (5) ◽  
pp. 797-800 ◽  
Author(s):  
Xiuli Shen ◽  
William S. Castle ◽  
Frederick G. Gmitter
Keyword(s):  
Acetic Acid ◽  
Shoot Proliferation ◽  
Shoot Tip ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Casuarina Cunninghamiana ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Proliferation

This study examined the effects of plant growth regulators, explant types, and their orientations on in vitro shoot proliferation of Casuarina cunninghamiana Miq. and also the subsequent rooting ability of shoots. Results showed that shoot proliferation occurred only in shoot tip explants cultured vertically on Murashige and Skoog (MS) medium supplemented with 2 or 4 μM thidiazuron (TDZ). Neither 6-benzylaminopurine alone nor in a combination with 1-naphthalene acetic acid (NAA) or gibberellic acid had any effect on shoot proliferation. TDZ at 4 μM resulted in the greatest percentage of axillary bud sprouting (70%) and mean number of sprouts per explant (2.3). Additionally, no shoot proliferation was observed from detipped or single-node explants or from horizontally placed shoot tip explants when cultured on the same TDZ-containing medium. The induced shoots produced adventitious roots on MS medium supplemented with 2.5, 5, or 10 μM indole-3-butyric acid (IBA), not with indole-3-acetic acid and NAA. Although the mean number of roots per explant was not significantly different between 2.5 and 5 μM IBA, the highest rooting percentage (68%) and mean length of roots per explant (0.7 cm) was achieved at 5 μM IBA. The current study provided preliminary information toward commercial in vitro propagation of Casuarina cunninghamiana male plants.

scholarly journals COMPARISON OF TWO METHODS FOR IN VITRO PROPAGATION OF RAUWOLFIA SERPENTINA FROM NODAL EXPLANTS

2007 ◽  
Vol 44 (07) ◽  
pp. 514-519 ◽  
Author(s):  
Ved Prakash Pandey ◽  
Jose Kudakasseril ◽  
Elizabeth Cherian ◽  
George Patani ◽  
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
Shoot Proliferation ◽  
Nodal Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
In Vitro Multiplication ◽  
Rauwolfia Serpentina ◽  
In Vitro Micropropagation

Two different methods of in vitro multiplication of Rauwolfia serpentina from nodal explants were compared viz. multiplication via callus morphogenesis and that via shoot proliferation from axillary buds. The second method was found to be far better. The optimum shoot proliferation occurred on Murashige and Skoog (MS) medium supplemented with 1 mg/L naphthalene acetic acid (NAA) and 2 mg/L of benzyl aminopurine (BAP). The best rooting of shoots occurred on MS medium containing 4% sucrose and 1 mg/L of NAA. Solid and liquid MS media were found to be similar in supporting shoot proliferation. The plants produced were successfully hardened and established in soil. An easy, reliable and reproducible protocol was developed for in vitro micropropagation of Rauwolfia serpentina from nodal explants.

scholarly journals Propagation Techniques for a Spineless Acacia wrightii

HortScience ◽  
2005 ◽  
Vol 40 (6) ◽  
pp. 1832-1837 ◽  
Author(s):  
Donita L. Bryan ◽  
Michael A. Arnold ◽  
R. Daniel Lineberger ◽  
W. Todd Watson
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Shoot Proliferation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Tissue Culture Propagation

Three spineless phenotypes of Acacia wrightii G. Bentham ex A. Gray were identified with aesthetic landscape potential. Experiments in seed, cutting, grafting, and tissue culture propagation were undertaken to perpetuate this desired spineless phenotype. Germination percentages for mechanically scarified seeds ranged from 33% to 94%, however yield of spineless seedlings was low (0% to 34%). Sulfuric acid scarification for 10, 20, 30, or 60 minutes hastened and unified germination compared to nontreated seeds by 7 to 8 days. Vegetative propagation was successful for softwood cuttings. Rooting measures increased with auxin (2:1 indole butyric acid to naphthalene acetic acid) concentrations from 0 to 15000 mg·L–1, with maximum rooting percentage (70%), root number (9.2), and root length (12.4 cm) per softwood cutting at 15000 mg·L–1 auxin 8 weeks after treatment. Rooting was not successful for semi-hardwood or hardwood cuttings. Whip-and-tongue or T-bud grafting was not successful. Tissue culture of shoots from in vitro germinated seedlings indicated that shoot proliferation was greatest in Murashige and Skoog (MS) medium with 15 μm zeatin. The number of shoots that rooted in vitro increased with increasing concentrations of indole-3-butyric acid from 0 to 25 μm.

scholarly journals Thidiazuron and Benzylamino Purine Stimulation of Apple Shoot Proliferation in Vitro

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 629e-629
Author(s):  
Karim H. Al-Juboory ◽  
Jabar H. Al-Niami
Keyword(s):  
Malus Domestica ◽  
Shoot Proliferation ◽  
Shoot Tip ◽  
Ms Medium ◽  
Proliferation In Vitro ◽  
Shoot Tip Explants ◽  
Apple Shoot ◽  
Stimulation Of

Thidiazuron (TDZ) and benzylamino purine stimulated shoot proliferation on shoot tip explants of wild apple (Malus domestica Borkh) when incorporated in Murashige and Skoog (MS) medium at concentrations of 1.0–10 μm. Shoot numbers obtained with TDZ were greater than the number produced when using BA in the medium but the shoots were shorter than with BA. Increasing TDZ levels increased shoot proliferation with 10 μm. Apple shoots were successfully rooted on MS medium with 2.0 mg·L–1 NAA and then transferred to a mixture of 1 peat: 1 perlite: 1 soil and acclimatized for potting.

scholarly journals High Frequency Plant Regeneration System of Aerides odorata Lour. Through Foliar and Shoot Tip Culture

2013 ◽  
Vol 41 (1) ◽  
pp. 169 ◽  
Author(s):  
Huidrom Sunitibala DEVI ◽  
Sanglakpam Irabati DEVI ◽  
Thingbaijam Dikash SINGH
Keyword(s):  
Shoot Tip ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Regeneration System ◽  
Direct Shoot Regeneration ◽  
Leaf Base ◽  
Clonal Multiplication ◽  
Commercially Important ◽  
Shoot Tip Explants

An efficient protocol for rapid clonal propagation from different explants of Aerides odorata Lour.- an endemic orchid of Manipur has been established. Leaf base explants showed significant response in ½ strength Murashige and Skoog medium supplemented with thidiazuron (TDZ) and 6-benzylaminopurine (BAP). Callus were initiated only from leaf base explants after 60 days of culture while other parts of leaves failed to response in all the treatments. Medium supplemented with 1.0 mg/L TDZ produced protocorm like bodies (PLBs) at the leaf base. Shoot tip explants of A. odorata showed different morphogenetic responses in different phytohormone treatments. Calli were initiated only in the medium containing α-naphthalene acetic acid (NAA). Highest calli frequency was observed in the medium containing 2 mg/L (NAA) (85.71±0.21) which indicates the importance of exogenous auxin in embryonic callus proliferation. Direct shoot regeneration on the other hand was observed in all the treatments. Highest number of shoot was obtained in higher concentration of NAA (2 mg/L) and BAP (4 mg/L) (4.80±0.18), showing combined effect of BAP and NAA, which may be due to the synergistic effect of cytokinin and auxin. Among the different rooting phytohormones, addition of NAA (0.5 mg/L) in ½ MS medium shows highest frequency of root induction. More than 95% in vitro plants survived during acclimatization under ex vitro conditions. This phytohormones and explants based micropropagation system can open up the route for in vitro clonal multiplication of this commercially important Aerides species.

Optimizing concentrations of growth regulators for in-vitro ginger propagation

2010 ◽  
Vol 27 (2) ◽  
pp. 61-65 ◽  
Author(s):  
Chukwuemeka Nkere ◽  
Egbichi Mbanaso
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Root Development ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Media Composition ◽  
Regeneration Rate ◽  
The Cost ◽  
Shoot Tip Explants

Optimizing concentrations of growth regulators for in-vitro ginger propagationThe study evaluates the performance of ginger in media containing different concentrations of growth regulators. Twenty-eight different treatment combinations of benzylaminopurine (BAP) and naphthalene acetic acid (NAA) incorporated into Murashige and Skoog (MS) medium were evaluated for optimal media composition for ginger micropropagation. The combination of 0.05 mgl-1 NAA and 4.0 mgl-1 BAP gave the highest shoot regeneration rate of 4.25. However, this did not differ significantly (p>0.05) from the result (3.38) from 0.05 mgl-1 NAA and 1.0 mgl-1 BAP. Considering the performance of the shoot tip explants in media and the need to lower the cost of micropropagation, the latter combination (0.05 mgl-1 NAA and 1.0 mgl-1 BAP) with 80 percent explant survival, gave an appropriate concentration of growth regulators in media composition for ginger propagation. This combination also supported root development, and perhaps would eliminate the stage of in vitro rooting.

scholarly journals Establishment of an efficient in vitro propagation protocol for Sumac (Rhus coriaria L.) and confirmation of the genetic homogeneity

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Saleh Amiri ◽  
Reza Mohammadi
Keyword(s):  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
High Rate ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Lateral Buds ◽  
Rhus Coriaria ◽  
Four Levels

AbstractThe conventional reproduction methods are not efficient for regeneration of Sumac (Rhus coriaria L.). The purpose of this work was to study the micropropagation of R. coriaria using lateral buds as explant in Murashige and Skoog (MS) medium with different concentrations of plant growth regulator (PGRs). Four concentrations of Benzylaminopurine (BAP) in combination with three concentrations of indol-3-butyric acid (IBA) and 1.0 mg/L gibberellic acid (GA3) were tested for establishment and shoot multiplication. For root induction, IBA was used at four levels combined with 0, 0.5 and 1 mg/L of naphthalene acetic acid (NAA) in full and half strength of MS medium. BAP at 2 mg/L with 1 mg/L IBA was best, with 88.88% of establishment. The highest shoot proliferation (12.30 ± 0.30) was obtained in medium fortified with 2 mg/L BAP plus 0.5 mg/L IBA and the highest shoot length (8.50 cm) was obtained at 3 mg/L BAP plus 1 mg/L IBA. The highest rooting (100%) was observed in 1/2-strength MS medium containing 1 mg/L IBA with 0.5 mg/L NAA. In conclusion, an efficient protocol with high rate of proliferation and rooting is described for R. coriaria, which can be used in massive propagation.

scholarly journals In vitro Shoot Proliferation through Cotyledonary Node and Shoot Tip Explants of Ficus religiosa L.

2017 ◽  
Vol 27 (1) ◽  
pp. 85-88 ◽  
Author(s):  
Mohsen Hesami ◽  
Mohammad Hosein Daneshvar ◽  
Amin Lotfi
Keyword(s):  
Shoot Proliferation ◽  
Cotyledonary Node ◽  
Shoot Tip ◽  
Ficus Religiosa ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Proliferation

Abstract not availablePlant Tissue Cult. & Biotech. 27(1): 85-88, 2017 (June)

scholarly journals Rapid In vitro Multiplication of Chirita longgangensis W.T. Wang: An Endemic and Endangered Gesneriaceae Species in China

HortScience ◽  
2007 ◽  
Vol 42 (3) ◽  
pp. 638-641 ◽  
Author(s):  
Zhenghui Tang ◽  
Honghui Lin ◽  
Lei Shi ◽  
Weilun Chen
Keyword(s):  
Acetic Acid ◽  
Somatic Embryos ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Basal Leaf ◽  
Second Stage ◽  
Regenerated Plantlets

Experiments were conducted to establish an efficient protocol for micropropagation of Chirita longgangensis W.T. Wang. Somatic embryos formed directly at the cut edges of C. longgangensis leaf explants on Murashige and Skoog (MS) medium supplemented with benzylaminopurine (BA) and α-naphthalene acetic acid (NAA). During the somatic embryo induction stage, leaf explants and basal leaf explants were used. Leaves were more appropriate explants than the basal leaf explants. The best medium was modified MS macronutrients and micronutrients supplemented with 0.5 mg·L−1 BA and 0.1 mg·L−1 NAA (the best mean number of somatic embryos per explants was 24.10 ± 1.63). The second stage was root induction and elongation. In vitro regenerated plantlets rooted best on MS medium containing 0.1 mg·L−1 indole-3-acetic acid (IAA) and 30 g·L−1 sucrose. Rooted plantlets, following acclimatization in a greenhouse, were successfully transferred to field conditions, and 95% of the plants survived. Application of this protocol has the ability for mass multiplication, in a limited time, of the endangered species C. longgangensis.

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