scholarly journals Season and Explant Origin Affect Phenolic Content, Browning of Explants, and Micropropagation of ×Malosorbus florentina (Zucc.) Browicz

HortScience ◽  
2013 ◽  
Vol 48 (1) ◽  
pp. 102-107 ◽  
Author(s):  
Aekaterini N. Martini ◽  
Maria Papafotiou ◽  
Stavros N. Vemmos
Keyword(s):  
Phenolic Content ◽  
Total Phenolics ◽  
Shoot Tip ◽  
Nodal Explants ◽  
Rare And Endangered ◽  
Shoot Tip Explants ◽  
Adult Plants ◽  
Compact Shape ◽  
Juvenile Plants

The aim of this study was to develop an efficient protocol for in vitro propagation of the rare and endangered ×Malosorbus florentina, not only enabling conservation of the species, but also its use as an ornamental. Explants excised from adult plants, shoot tip explants, and explants collected in March and April showed more browning and had higher content of total phenolics than explants excised from juvenile tissue, nodal explants, and those collected during any of the other months of the year. Shoot tip explants from adult plants were more difficult to establish in vitro (14%) compared with explants from micropropagated plantlets or sprouts of burned plants (29% to 36%). Nodal explants excised from seedlings were established at the highest percentage (83%), giving the most shoots per explant (5.2). Generally, in vitro cultures established from adult plants, with the exception of one culture, showed lower multiplication rates compared with cultures from juvenile plants. Nodal explants from the base of sprouts produced a higher percentage (60%) of shoots than explants from upper locations (20% to 31%), but any differences in proliferation rates of established cultures ceased after the third subculture. Microshoots from juvenile cultures were more capable of rooting (51% to 58%) than were those from adult plants (16% to 32%), whereas 83% of the plantlets were acclimatized ex vitro independently of their origin, but plantlets of juvenile origin, although developing the same height as those originating from adult plants, had shorter internodes and thus more compact shape.

scholarly journals In vitro Mass Propagation of Artemisia (Artemisia annua L.) cv: Anamed

2014 ◽  
Vol 23 (2) ◽  
pp. 165-176 ◽  
Author(s):  
Tilahun Hailu ◽  
Balcha Abera ◽  
Gabra Mariam
Keyword(s):  
Large Scale ◽  
Artemisia Annua ◽  
Shoot Tip ◽  
Nodal Explants ◽  
Mass Propagation ◽  
Mean Values ◽  
Coffee Husk ◽  
Soil Mix ◽  
Shoot Tip Explants

An efficient in vitro propagation protocol was developed for anamed (A-3) cultivar of Artemisia annua. Two and 1.5% concentration of NaOCl treatment for 10 and 20 min were found to be optimum for sterilization of shoot tip and nodal explants, respectively. Maximum percentage (98.75 ± 2.50) shoot induction was observed from nodal explants cultured on MS supplemented with 0.8 mg/l BAP + 0.1 mg/l IBA  followed by 82.50 ± 2.88% from shoot tip explants on the same medium with 0.8 mg/l TDZ for shoot tip explants. The highest number of shoots (8.05 ± 0.66/explant) was regenerated on MS + 1 mg/l BAP + 0.1 mg/l IBA. Best rooting with mean values of 18.25 ± 0.95/explant root number and root length (6.35 ± 0.10 cm) was recorded on 1/2 MS + 0.5 mg/l IBA. Up on acclimation and transplanting, 80% survival efficiency was observed on the soil mix ratio of  2 : 1 : 1 (decomposed coffee husk, forest soil and sand, respectively). The developed regeneration protocol enables a large scale commercial production and a possible system towards the genetic improvement of this crop. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17518 Plant Tissue Cult. & Biotech. 23(2): 165-176, 2013  (December)

scholarly journals Clonal Propagation In Vitro of Paphiopedilum Hybrids from Adult Plants

HortScience ◽  
2019 ◽  
Vol 54 (9) ◽  
pp. 1565-1569
Author(s):  
Vi Nguyen Tuong Do ◽  
Shan-Te Hsu ◽  
Yung-I Lee
Keyword(s):  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Shoot Tip ◽  
Seasonal Effect ◽  
Optimal Timing ◽  
Axillary Shoot ◽  
Shoot Bud ◽  
Shoot Tip Explants ◽  
Adult Plants

The aim of this study was to develop an efficient protocol for shoot tip culture from adult plants of Paphiopedilum Pfitzer. A considerable seasonal effect on explant collection was observed in the aseptic cultures established from adult plants, including the survival and microbial contamination of explants. The shoot tip explants excised from adult plants in February and May showed higher survival and had less contamination than those explants excised in August and November. Moreover, the season of explant collection also affected the subsequent shoot forming capacity and multiplication of axillary buds. In Paphiopedilum ‘In-Charm Silver Bell’, higher shoot forming capacity was observed in February and May, whereas higher shoot multiplication was observed only in February. In Paphiopedilum ‘Hsinying Maudiae Leopard’, both February and May were optimal timing for shoot forming capacity and multiplication. We also demonstrated the effectiveness of transcinnamic acid (tCA), an antiauxin chemical in diminishing the apical dominance of shoot tip explant and thus improving the axillary bud outgrowth. In P. ‘In-Charm Silver Bell’, the addition of 100 μM tCA plus 13.3 μM 6-benzylaminopurine (BA) for 1 month promoted axillary shoot bud formation from shoot tip explants as compared with the control.

scholarly journals In vitro propagation and cytological analysis of Sophora mollis Royle: an endangered medicinal shrub

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Aakriti Bhandari ◽  
Harminder Singh ◽  
Amber Srivastava ◽  
Puneet Kumar ◽  
G. S. Panwar ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Average Length ◽  
Germplasm Conservation ◽  
Basic Chromosome Number ◽  
Shoot Tip ◽  
Ex Situ ◽  
Ms Medium ◽  
Basic Chromosome ◽  
Shoot Tip Explants

Abstract Background Sophora mollis Royle (family Fabaceae, subfamily-Papilionaceae) is a multipurpose legume distributed in plains and foothills of the North-West Himalaya to Nepal and is facing high risk of extinction due to habitat loss and exploitation by the local people for its fuel and fodder values. Therefore, the present study was conducted to standardize a micropropagation protocol for Sophora mollis by using shoot tip explants and to study the meiotic chromosome count in the species. Results Multiple shoots were induced in shoot tip explants of Sophora mollis in Murashige and Skoog medium supplemented with different concentrations of cytokinins alone (BAP, TDZ, and Kinetin) and in combination with varying concentrations of NAA. MS medium supplemented with BAP (8.9 μM) was observed to be the optimal medium for multiple shoot induction and maximum 25.32 shoots per explant was obtained with average length of 4.5 ± 0.8 cm. In vitro developed shoots were transferred onto rooting media supplemented with different concentrations of auxin (IAA, IBA, and NAA). Maximum 86% rooting was observed in half-strength MS medium supplemented with 21.20 μM NAA with an average of 21.26 roots per culture. In vitro raised plantlets were adapted to greenhouse for better acclimatization and 60% plants were successfully transferred to the open environment. Based on the chromosome counts available from the literature and the current study, the species tend to show a basic chromosome number of x = 9. Conclusion The micropropagation protocol standardized can be helpful for the ex situ mass multiplication and germplasm conservation of the endangered species. Moreover, the ex situ conservation approach will be helpful in actively bridging the gap between ex situ and in situ approaches through the reintroduction of species in the wild. The cytological studies revealed the basic chromosome number x = 9 of the species.

scholarly journals An Improved Protocol for Multiple Shoot Regeneration from Seedlings and Mature Explants of Citrus macroptera Mont.

2009 ◽  
Vol 18 (1) ◽  
pp. 17-24
Author(s):  
Md. Nesawar Miah ◽  
Shahina Islam ◽  
Syed Hadiuzzaman
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Cow Dung ◽  
Half Strength ◽  
Multiple Shoot Regeneration ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Regeneration

Efforts have been made to establish a protocol for direct multiple shoot regeneration from both in vitro grown seedlings and mature plants of Citrus macroptera. Both nodal and shoot tip explants taken from in vitro grown seedlings were cultured in MS supplemented with different concentrations of BAP and Kn either singly or in combinations. Both these explants are capable to regenerate and produce in vitro multiple shoots. Maximum number of shoots were obtained from nodal explants in MS supplemented with 1.0 mg/l BAP. BAP alone was found superior to Kn. On the other hand, only nodal explants from mature plants were used and 1.0 mg/1 BAP was also found best suitable for shoot induction and multiplication. Ex vitro rooting in pot soil (mixed with biogas slurry derived from cow-dung) was most successful compared to in vitro rooting in half strength of MS supplemented with different concentrations of NAA and IBA. Key words: In vitro, Shoot regeneration, Citrus macroptera D.O.I. 10.3329/ptcb.v18i1.3246 Plant Tissue Cult. & Biotech. 18(1): 17-24, 2008 (June)

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

scholarly journals In vitro Mass Propagation of a Ground Orchid - Phaius tancarvilleae (L'Her.) Blume through Shoot Tip Culture

2011 ◽  
Vol 21 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha
Keyword(s):  
High Frequency ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Shoot Tip ◽  
Mass Propagation ◽  
Shoot Tip Culture ◽  
Shoot Tip Explants ◽  
Direct Shoot

High frequency direct shoot proliferation was induced from the shoot tip explants derived from the in vitro grown seedlings of a critically endangered and horticulturally important ground orchid Phaius tancarvilleae (L'Her) Blume. Shoot tip explants cultured on solidified MS with alone or combination of various concentrations of NAA and BAP produced shoots and multiple shoots. The maximum number of healthy shoots was observed on MS with BAP (1.0 mg/l) with an average of 13.3 shoots per culture in 20 weeks; where shoot multiplication was initiated after 4 weeks of culture. Regenerated shoots rooted on MS with various concentrations of NAA, IAA, IBA. MS with NAA (0.5 mg/l) was the most appropriate condition for rooting. The well developed in vitro rooted plantlets were hardened successfully in the potting mixture containing cocopeat and sphagnum moss in the ratio of 2 : 1.   Key words: Mass propagation, Phaius tancarvilleae, shoot multiplication   D. O. I. 10.3329/ptcb.v21i2.10241   Plant Tissue Cult. & Biotech. 21(2): 181-188, 2011 (December)

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