scholarly journals Clonal Propagation In Vitro of Paphiopedilum Hybrids from Adult Plants

HortScience ◽  
2019 ◽  
Vol 54 (9) ◽  
pp. 1565-1569
Author(s):  
Vi Nguyen Tuong Do ◽  
Shan-Te Hsu ◽  
Yung-I Lee
Keyword(s):  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Shoot Tip ◽  
Seasonal Effect ◽  
Optimal Timing ◽  
Axillary Shoot ◽  
Shoot Bud ◽  
Shoot Tip Explants ◽  
Adult Plants

The aim of this study was to develop an efficient protocol for shoot tip culture from adult plants of Paphiopedilum Pfitzer. A considerable seasonal effect on explant collection was observed in the aseptic cultures established from adult plants, including the survival and microbial contamination of explants. The shoot tip explants excised from adult plants in February and May showed higher survival and had less contamination than those explants excised in August and November. Moreover, the season of explant collection also affected the subsequent shoot forming capacity and multiplication of axillary buds. In Paphiopedilum ‘In-Charm Silver Bell’, higher shoot forming capacity was observed in February and May, whereas higher shoot multiplication was observed only in February. In Paphiopedilum ‘Hsinying Maudiae Leopard’, both February and May were optimal timing for shoot forming capacity and multiplication. We also demonstrated the effectiveness of transcinnamic acid (tCA), an antiauxin chemical in diminishing the apical dominance of shoot tip explant and thus improving the axillary bud outgrowth. In P. ‘In-Charm Silver Bell’, the addition of 100 μM tCA plus 13.3 μM 6-benzylaminopurine (BA) for 1 month promoted axillary shoot bud formation from shoot tip explants as compared with the control.

scholarly journals In vitro Shoot Multiplication of Bupleurum disticho-phyllum Wight - A Native Medicinal Plant of Southern India

1970 ◽  
Vol 17 (2) ◽  
pp. 115-124 ◽  
Author(s):  
S. Karuppusamy ◽  
T. Pullaiah
Keyword(s):  
Medicinal Plant ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Shoot Tip ◽  
Axillary Shoot ◽  
Axillary Shoots ◽  
Axillary Shoot Proliferation ◽  
Shoot Tip Explants

Shoot multiplication of Bupleurum distichophyllum was achieved from the nodal and shoot tip explants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum explant response was from axillary shoots and the highest number of shoots per explant was obtained on MS fortified with 1.0 mg/l BAP. The highest degree of axillary shoot proliferation was found to be 74 and 70% for nodal- and shoot tip explants, respectively on the medium containing 1.0 mg/l BAP + 0.1 mg/l NAA. The combination of BAP and GA3 was also found to be effective for both type of explants. The degree of shoot formation was affected by explant types and the exogenous hormonal regime in the medium. The regenerated shoots were successfully rooted on MS supplemented with 2.0 mg/l IBA, after sequential hardening, survival rate was 71%. Key words: Bupleurum distichophyllum, Medicinal plant, Micropropagation, Conservation Plant Tissue Cult. & Biotech. 17(2): 115-124, 2007 (December) DOI: 10.3329/ptcb.v17i2.2574

scholarly journals 586 PB 123 AXILLARY SHOOT REGENERATION IN CHINKAPIN OAK

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 515g-515
Author(s):  
Sudeep Vyapari ◽  
Houchang Khatamian
Keyword(s):  
Shoot Regeneration ◽  
Shoot Multiplication ◽  
Shoot Tip ◽  
Axillary Shoot ◽  
Ms Medium ◽  
Pulse Treatment ◽  
Axillary Shoots ◽  
Shoot Tip Explants

Surface disinfested nodal and shoot-tip sections of chinkapin oak (Quercus muehlenbergii Engelm.), obtained from adult or juvenile source, when cultured on WFM supplemented with BA or kinetin (1.0 -5.0 mg l-1) produced greater number of axillary shoots per explant and shoot lengths than MS medium. Nodal and shoot-tip explants cultured in WPM containing cytokinins, BA or kinetin (0.1 - 5.0 mg l“) resulted in greater number of axillary shoots than media containing auxins, 2,4-D or NAA (1.0 - 5.0 mg l-1). In vitro grown shoot explants cultured in WFM shoot multiplication medium containing thidiazuron did not produce axillary shoots. Microshoots when cultured in WFM plus NAA or IBA (0.1 -2.0 mg l-1), or subjected to IBA (0.5 mg l-1) pulse treatment (0, 5, 10 or 15 min.) did not root.

scholarly journals In vitro Mass Propagation of a Ground Orchid - Phaius tancarvilleae (L'Her.) Blume through Shoot Tip Culture

2011 ◽  
Vol 21 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha
Keyword(s):  
High Frequency ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Shoot Tip ◽  
Mass Propagation ◽  
Shoot Tip Culture ◽  
Shoot Tip Explants ◽  
Direct Shoot

High frequency direct shoot proliferation was induced from the shoot tip explants derived from the in vitro grown seedlings of a critically endangered and horticulturally important ground orchid Phaius tancarvilleae (L'Her) Blume. Shoot tip explants cultured on solidified MS with alone or combination of various concentrations of NAA and BAP produced shoots and multiple shoots. The maximum number of healthy shoots was observed on MS with BAP (1.0 mg/l) with an average of 13.3 shoots per culture in 20 weeks; where shoot multiplication was initiated after 4 weeks of culture. Regenerated shoots rooted on MS with various concentrations of NAA, IAA, IBA. MS with NAA (0.5 mg/l) was the most appropriate condition for rooting. The well developed in vitro rooted plantlets were hardened successfully in the potting mixture containing cocopeat and sphagnum moss in the ratio of 2 : 1.   Key words: Mass propagation, Phaius tancarvilleae, shoot multiplication   D. O. I. 10.3329/ptcb.v21i2.10241   Plant Tissue Cult. & Biotech. 21(2): 181-188, 2011 (December)

scholarly journals Season and Explant Origin Affect Phenolic Content, Browning of Explants, and Micropropagation of ×Malosorbus florentina (Zucc.) Browicz

HortScience ◽  
2013 ◽  
Vol 48 (1) ◽  
pp. 102-107 ◽  
Author(s):  
Aekaterini N. Martini ◽  
Maria Papafotiou ◽  
Stavros N. Vemmos
Keyword(s):  
Phenolic Content ◽  
Total Phenolics ◽  
Shoot Tip ◽  
Nodal Explants ◽  
Rare And Endangered ◽  
Shoot Tip Explants ◽  
Adult Plants ◽  
Compact Shape ◽  
Juvenile Plants

The aim of this study was to develop an efficient protocol for in vitro propagation of the rare and endangered ×Malosorbus florentina, not only enabling conservation of the species, but also its use as an ornamental. Explants excised from adult plants, shoot tip explants, and explants collected in March and April showed more browning and had higher content of total phenolics than explants excised from juvenile tissue, nodal explants, and those collected during any of the other months of the year. Shoot tip explants from adult plants were more difficult to establish in vitro (14%) compared with explants from micropropagated plantlets or sprouts of burned plants (29% to 36%). Nodal explants excised from seedlings were established at the highest percentage (83%), giving the most shoots per explant (5.2). Generally, in vitro cultures established from adult plants, with the exception of one culture, showed lower multiplication rates compared with cultures from juvenile plants. Nodal explants from the base of sprouts produced a higher percentage (60%) of shoots than explants from upper locations (20% to 31%), but any differences in proliferation rates of established cultures ceased after the third subculture. Microshoots from juvenile cultures were more capable of rooting (51% to 58%) than were those from adult plants (16% to 32%), whereas 83% of the plantlets were acclimatized ex vitro independently of their origin, but plantlets of juvenile origin, although developing the same height as those originating from adult plants, had shorter internodes and thus more compact shape.

scholarly journals Micropropagation of Cymbidium iridioides

2012 ◽  
Vol 12 ◽  
pp. 91-96 ◽  
Author(s):  
Bijaya Pant ◽  
Sangeeta Swar
Keyword(s):  
Shoot Multiplication ◽  
Shoot Tip ◽  
Naphthaleneacetic Acid ◽  
Ideal Condition ◽  
Short Period ◽  
Single Shoot ◽  
The Media ◽  
Over Exploitation ◽  
Shoot Tip Explants

Seeds of Cymbidium iridioides D. Don, a highly collected orchid species of Nepal, has been developed were cultured on Murashige and Skoog, 1962 (MS) static media solidified with 0.8% agar. Complete seedlings were obtained from asymbiotically germinated seeds on MS media supplemented with 6- benzyalaminopurine (BAP) 1 mg/l plus ?-naphthaleneacetic acid (NAA) 1 mg/l in eight weeks of primary culture. The shoot tip explants were obtained from in vitro grown seedling for the shoot multiplication. Maximum number of shoots were developed from shoot tip explants on MS media supplemented with BAP 0.5 mg/l (8.25 shoots per single shoot). This ability did not decline even after a year of subculture. For rooting of shoots the media was supplemented with various auxins. Media supplemented with 1mg/l indole-3-butyric acid (IBA) was the ideal condition for root formation. Thus obtained complete plantlets were able to grow in community pots after a short period of acclimatization. This result suggests that the methodology might be applied as an alternative to minimize the over exploitation of the natural population of this species.DOI: http://dx.doi.org/10.3126/njst.v12i0.6485 Nepal Journal of Science and Technology 12 (2011) 91-96 

scholarly journals In vitro propagation and cytological analysis of Sophora mollis Royle: an endangered medicinal shrub

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Aakriti Bhandari ◽  
Harminder Singh ◽  
Amber Srivastava ◽  
Puneet Kumar ◽  
G. S. Panwar ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Average Length ◽  
Germplasm Conservation ◽  
Basic Chromosome Number ◽  
Shoot Tip ◽  
Ex Situ ◽  
Ms Medium ◽  
Basic Chromosome ◽  
Shoot Tip Explants

Abstract Background Sophora mollis Royle (family Fabaceae, subfamily-Papilionaceae) is a multipurpose legume distributed in plains and foothills of the North-West Himalaya to Nepal and is facing high risk of extinction due to habitat loss and exploitation by the local people for its fuel and fodder values. Therefore, the present study was conducted to standardize a micropropagation protocol for Sophora mollis by using shoot tip explants and to study the meiotic chromosome count in the species. Results Multiple shoots were induced in shoot tip explants of Sophora mollis in Murashige and Skoog medium supplemented with different concentrations of cytokinins alone (BAP, TDZ, and Kinetin) and in combination with varying concentrations of NAA. MS medium supplemented with BAP (8.9 μM) was observed to be the optimal medium for multiple shoot induction and maximum 25.32 shoots per explant was obtained with average length of 4.5 ± 0.8 cm. In vitro developed shoots were transferred onto rooting media supplemented with different concentrations of auxin (IAA, IBA, and NAA). Maximum 86% rooting was observed in half-strength MS medium supplemented with 21.20 μM NAA with an average of 21.26 roots per culture. In vitro raised plantlets were adapted to greenhouse for better acclimatization and 60% plants were successfully transferred to the open environment. Based on the chromosome counts available from the literature and the current study, the species tend to show a basic chromosome number of x = 9. Conclusion The micropropagation protocol standardized can be helpful for the ex situ mass multiplication and germplasm conservation of the endangered species. Moreover, the ex situ conservation approach will be helpful in actively bridging the gap between ex situ and in situ approaches through the reintroduction of species in the wild. The cytological studies revealed the basic chromosome number x = 9 of the species.

scholarly journals Influence of Cytokinins in Combination with GA3on Shoot Multiplication and Elongation of Tea Clone Iran 100 (Camellia sinensis(L.) O. Kuntze)

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Reza Azadi Gonbad ◽  
Uma Rani Sinniah ◽  
Maheran Abdul Aziz ◽  
Rosfarizan Mohamad
Keyword(s):  
Camellia Sinensis ◽  
Woody Plants ◽  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Liquid Form ◽  
Solid Media

The use ofin vitroculture has been accepted as an efficient technique for clonal propagation of many woody plants. In the present research, we report the results of a number of experiments aimed at optimizing micropropagation protocol for tea (Camellia sinensis(L.) O. Kuntze) (clone Iran 100) using nodal segments as the explant. The effect of different combinations and concentrations of plant growth regulators (PGR) (BAP, TDZ, GA3) on shoot multiplication and elongation was assessed. The influence of exposure to IBA in liquid form prior to transfer to solid media on rooting of tea microshoots was investigated. The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA3. TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L. Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

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