scholarly journals Response of Some Watermelon Accessions to a Puerto Rico Strain of Zucchini yellow mosaic virus

HortScience ◽  
2020 ◽  
Vol 55 (9) ◽  
pp. 1509-1514
Author(s):  
Giseiry Rosa-Valentín ◽  
Linda Wessel-Beaver ◽  
Jose Carlos V. Rodrigues
Keyword(s):  
Puerto Rico ◽  
Mosaic Virus ◽  
Symptom Severity ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Accession Number ◽  
Department Of Agriculture ◽  
Plant Breeder ◽  
Severity Scores

One of the most important members of the Potyviridae is Zucchini yellow mosaic virus (ZYMV). It affects watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] as well as other cucurbits in most parts of the world. Although several genotypes have been reported as having resistance to ZYMV, differential responses to ZYMV strains are known to occur. Using a Puerto Rico strain of ZYMV (ZYMV-PR, GenBank accession number MN422959), we tested the response of 11 genotypes [PIs from the U.S. Department of Agriculture (USDA) National Genetic Resources Program] previously reported as having resistance to this virus. In two greenhouse trials, the first three leaves of seedlings of each genotype were mechanically inoculated with ZYMV-PR. An enzyme-linked immunosorbent assay (ELISA) was done on each seedling’s fourth leaf and symptom severity was rated on the first, third, fifth, and seventh leaves. There were significant genotype × trial interactions for most variables, but some genotypes performed consistently in both trials. All seedlings of PI 537277 tested negative for ELISA (absorbance < 0.200) across both trials. PI 537277, PI 595200, PI 595201, and PI 595203 were generally among the accessions with the lowest symptom severity scores. Overall, symptom severity correlated poorly with ELISA readings. But all plants of PI 537277, and most plants of PI 595201 and PI 595203, had low ELISA readings and low severity scores. Despite having low severity scores, PI 595200 was among the genotypes with the highest ELISA readings in trial 2. For the plant breeder, the most useful genotypes are those that exhibit reduced severity as well as low ELISA. PI 537277, PI 595201, and PI 595203 met those criteria in this study. Of these three accessions, PI 595203 would be the most useful in a breeding program because it has shown resistance to the Puerto Rico, Florida, and China strains of ZYMV.

scholarly journals Screening for Resistance to Zucchini yellow mosaic virus in the Watermelon Germplasm

HortScience ◽  
2019 ◽  
Vol 54 (2) ◽  
pp. 206-211 ◽  
Author(s):  
Nihat Guner ◽  
Zvezdana Pesic-VanEsbroeck ◽  
Luis A. Rivera-Burgos ◽  
Todd C. Wehner
Keyword(s):  
Mosaic Virus ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
Germplasm Collection ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Department Of Agriculture ◽  
Germplasm Screening ◽  
Disease Rating ◽  
The U.S

The Florida strain of Zucchini yellow mosaic virus (ZYMV-FL) is one of the major viral diseases of watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai]. Although some screening has been performed for ZYMV resistance in watermelon, the germplasm collection has not been screened extensively for resistance. The objectives of this study were to screen the U.S. Department of Agriculture (USDA) watermelon germplasm collection for ZYMV resistance and to verify the disease rating for the most resistant and most susceptible accessions. We used a randomized complete block with four replications, 1613 PI accessions, and 41 cultivars. ‘Charleston Gray’ and ‘Crimson Sweet’ susceptible controls were used to verify that the ZYMV inoculum was virulent. After the last rating, an enzyme-linked immunosorbent assay (ELISA) was performed to determine the presence of a virus in the plant tissue. The PI accessions with high resistance to ZYMV-FL that also exhibited resistance to other watermelon viruses were PI 595203, PI 386015, PI 386016, PI 386024, PI 386025, PI 386026, PI 244018, PI 244019, PI 485583, PI 494528, and PI 494529. The ZYMV-FL retest of the most resistant 46 PI accessions showed that there were some escapes. Sixteen resistant PI accessions had a rating of 3.0 or less for the average and maximum ratings: PI 595203, PI 537277, PI 560016, PI 386016, PI 386019, PI 485580, PI 494529, PI 595200, PI 494528, PI 595201, PI 386025, PI 494530, PI 386015, PI 386021, PI 386026, and PI 596662. Overall, PI 595203 had the highest resistance according to both the germplasm screening and the retest studies.

scholarly journals Development of Bottle Gourd Lines Resistant to Zucchini Yellow Mosaic Virus Using Ethyl Methanesulfonate Mutagenesis

HortScience ◽  
2021 ◽  
pp. 1-7
Author(s):  
Asma Mohammed Saeed Al-Kubati ◽  
Baoshan Kang ◽  
Liming Liu ◽  
Aqleem Abbas ◽  
Qinsheng Gu
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Ethyl Methanesulfonate ◽  
Bottle Gourd ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Practical Strategy ◽  
Resistant Lines ◽  
Polymerase Chain ◽  
Das Elisa

Zucchini yellow mosaic virus (ZYMV) causes serious damage to cucurbit crops worldwide and can be spread by aphids, by mechanical injury, and in seeds. With the popularization of cucurbit grafting, the use of susceptible rootstock has increased the risk of ZYMV infection in cucurbit crops. In China, the bottle gourd (Lagenaria siceraria) is a widely used rootstock in grafted watermelon production. However, few resistant bottle gourds are available commercially. This study developed bottle gourd lines resistant to ZYMV using ethyl methanesulfonate (EMS) mutagenesis. A new mutated bottle gourd population (M1) was generated by treating seeds with EMS. Diverse phenotypes were observed in the seedlings, flowers, and fruit of M2 plants, some of which are of potential commercial interest, such as dwarfing and different fruit shapes. Based on the M2 phenotypes, 106 M3 lines were selected and screened for resistance to ZYMV by mechanical inoculation and agroinfiltration. Nine M3 lines were resistant to ZYMV during three tests. One inbred M4 line (177-8) was developed and showed stable resistance and no virus when tested using a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and polymerase chain reaction. These resistant lines are promising materials for developing watermelon rootstock and exploring resistance genes as new ZYMV-resistant resources. EMS induction could be a practical strategy for creating resistant cucurbit crops.

scholarly journals Occurrence and Distribution of Pumpkin and Squash Viruses in Illinois

Plant Disease ◽  
2008 ◽  
Vol 92 (1) ◽  
pp. 61-68 ◽  
Author(s):  
S. Jossey ◽  
M. Babadoost
Keyword(s):  
Virus Infection ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Dual Infection ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Virus Infections ◽  
Dark Green

Surveys were conducted during 2004 to 2006 to identify the viruses infecting pumpkin and squash in Illinois. In 2004, 16 jack-o-lantern pumpkin (Cucurbita pepo) samples and one squash (C. pepo) sample were collected from 11 counties. In 2005, 85 jack-o-lantern pumpkin, 12 processing pumpkin (Cucurbita moschata), 37 squash, and six gourd (C. pepo) samples were collected from 54 counties. In 2006, 85 jack-o-lantern pumpkin, 16 processing pumpkin, 51 squash, and 18 gourd samples were collected from 47 counties. Cucumber mosaic virus (CMV), Papaya ringspot virus (PRSV), Squash mosaic virus (SqMV), Tobacco ringspot virus (TRSV), Tomato ringspot virus (ToRSV), Watermelon mosaic virus (WMV), Zucchini yellow mosaic virus (ZYMV), and unknown potyviruses were detected in pumpkin, squash, and gourd fields during the surveys, using enzyme-linked immunosorbent assay (ELISA). Overall, 86, 11, 75, and 79% of jack-o-lantern pumpkin, processing pumpkin, squash, and gourds, respectively, were tested positive for virus infection during the survey. WMV was detected in 47, 46, and 52% of the samples in 2004, 2005, and 2006, respectively, and was the most prevalent virus throughout the state. SqMV was detected in more counties than any other virus because it was identified in 65 and 88% of the counties surveyed in 2005 and 2006, respectively. SqMV was detected in 6, 41, and 48% of the samples in 2004, 2005, and 2006, respectively. During the surveys, CMV was detected in 6, 4, and 3% of the samples; PRSV was detected in 6, 11, and 4% of the samples; and ZYMV was detected in 18, 4, and 4% of the samples tested in 2004, 2005, and 2006, respectively. TRSV was detected in 3% of the samples in 2005, for the first time on pumpkin in Illinois. Pathogenicity of the detected viruses was proved for CMV, PRSV, SqMV, WMV, and ZYMV on summer squash (‘Fortune’ and ‘Grey Zucchini’), jack-o-lantern pumpkin (‘Howden’), and processing pumpkin (‘Dickinson’). All of the viruses were present alone and mixed in the samples tested. Earlier in the growing seasons (July and early August), single-virus infections were detected. Mixed infections were more common from the second week of August until the end of the growing season in October. Dual infection of WMV and SqMV was the most prevalent mixed virus infection detected in Illinois. Most viruses infecting pumpkin and squash showed similar symptoms. The most common symptoms observed in the commercial fields and in the greenhouse studies were light- and dark-green mosaic, veinbanding, veinclearing, puckering, and deformation of leaves of pumpkin, squash, and gourds. Severe symptoms included fernleaf and shoestring on leaves and color breaking and deformation of fruit.

scholarly journals Discriminating Mutations of HC-Pro of Zucchini yellow mosaic virus with Differential Effects on Small RNA Pathways Involved in Viral Pathogenicity and Symptom Development

2010 ◽  
Vol 23 (1) ◽  
pp. 17-28 ◽  
Author(s):  
Hui-Wen Wu ◽  
Shih-Shun Lin ◽  
Kuan-Chun Chen ◽  
Shyi-Dong Yeh ◽  
Nam-Hai Chua
Keyword(s):  
Amino Acids ◽  
Gene Silencing ◽  
Mosaic Virus ◽  
Symptom Severity ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Transcriptional Gene Silencing ◽  
Molecular Evidence ◽  
Virus Induced Gene Silencing ◽  
Conserved Amino Acids

Helper component-proteinase (HC-Pro), the gene-silencing suppressor of Potyvirus spp., interferes with microRNA (miRNA) and short-interfering RNA (siRNA) pathways. Our previous studies showed that three mutations of highly conserved amino acids of HC-Pro, R180I (mutation A), F205L (B), and E396N (C), of Zucchini yellow mosaic virus (ZYMV) affect symptom severity and viral pathogenicity. The mutant ZYMV GAC (ZGAC) with double mutations, R180I/E396N, induces transient leaf mottling in host plants followed by recovery. This mutant confers complete cross protection against subsequent infection by the parental ZYMV (ZG) strain. Here, we sought to obtain molecular evidence on the roles of the three highly conserved amino acids of HC-Pro in miRNA and siRNA pathways using transgenic Arabidopsis plants expressing comparable levels of wild-type and mutant HC-Pro proteins. We demonstrated that amino acid residues 180, 205, and 396 of HC-Pro are critical for suppression of miRNA, trans-acting siRNA (ta-siRNA), and virus-induced gene silencing (VIGS) pathways but not for sense-post transcriptional gene silencing (s-PTGS). Because the HC-Pro double mutant (R180I/E396N) does not interfere with miRNA and ta-siRNA pathways, the ZGAC mutant virus elicits only attenuated symptoms. Furthermore, the recovery seen on ZGAC-infected plants likely results from the weak VIGS suppression by the HC-Pro double AC mutant. Thus, through manipulating these three conserved amino acids on HC-Pro, symptom severity of diseases caused by Potyvirus spp. can be modulated to generate useful cross protectants for field application. Although some of our mutated HC-Pro proteins do not interfere with miRNA and ta-siRNA pathways, they still retain the ability to suppress s-PTGS.

scholarly journals Occurrence, Distribution, and Relative Incidence of Seven Viruses Infecting Greenhouse-Grown Cucurbits in Iran

Plant Disease ◽  
2007 ◽  
Vol 91 (2) ◽  
pp. 159-163 ◽  
Author(s):  
Hossain Massumi ◽  
Asghar Samei ◽  
Akbar Hosseini Pour ◽  
Mehdi Shaabanian ◽  
Heshmetollah Rahimian
Keyword(s):  
Mosaic Virus ◽  
Natural Infection ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Fruit Samples ◽  
Cucumber Necrosis Virus ◽  
Papaya Ring Spot Virus ◽  
Wilt Virus

Greenhouse-grown cucurbit crops in several Iranian regions were surveyed for the incidence of Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ring spot virus-type W (PRSV-W), Watermelon mosaic virus-2 (WMV-2), Zucchini yellow mosaic virus (ZYMV), Cucumber necrosis virus (CuNV) and Tomato spotted wilt virus (TSWV) from September 2002 to June 2004. In all, 1,304 random and 1,085 symptomatic leaf or fruit samples were collected. Samples were analyzed for virus infection by enzyme-linked immunosorbent assay. CMV and ZYMV were the viruses most frequently detected, accounting for 21.2 and 18% of the infected plants, respectively. WMV-2 was detected with 4.3% incidence in 15 regions and TSWV with 1.25% incidence only in 2 regions. CuNV, SqMV, and PRSV-W were not detected in any samples. Double and triple infections involving different combinations of CMV, ZYMV, WMV-2, and TSWV were noted in 117 and 4 samples, respectively. Natural infection of cucumber with TSWV and ZYMV is reported for the first time from Iran. Of 16 plant species from 14 genera, growing in or around greenhouse facilities, 6 were found infected with ZYMV, TSWV, WMV-2, and CMV and may act as reservoirs of the viruses. Four species are reported as new hosts of these viruses.

scholarly journals Cucumber green mottle mosaic virus in agroecosystems of Ukraine

2018 ◽  
Vol 76 (2) ◽  
pp. 71-78
Author(s):  
T. Rudnieva ◽  
T. Shevchenko ◽  
A. Shevchenko ◽  
I. Budzanivska
Keyword(s):  
Mosaic Virus ◽  
Open Field ◽  
Zucchini Yellow Mosaic Virus ◽  
Field Conditions ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Vegetable Crops ◽  
Seed Material ◽  
Planting Material

Cucumber green mottle mosaic virus (CGMMV) is a widespread pathogen capable of infecting plants cultivated in both greenhouse and openfield conditions with equal efficiency. The host range of CGMMV is restricted to cucurbit plants whereas induced crop losses may reach 25–50 %. Despite the wide array of available antiviral techniques, CGMMV could not be completely removed from the seed/planting material. In Ukraine, Cucumber green mottle mosaic virus occurs almost exclusively on greenhouse cucumbers. However, data from other countries suggest wide spread of CGMMV on watermelons, melons, cucumbers and squashes grown in both greenhouse and open-field conditions. In this view and taking into account high pathogenicity of CGMMV, we aimed at studying spread of Cucumber green mottle mosaic virus in Ukraine on cucurbit plants in the open-field conditions. Methods: enzyme-linked immunosorbent assay, electron microscopy, statistical methods. Results : Spread of Cucumber green mottle mosaic virus (CGMMV) on cucurbit vegetable crops grown in open-field conditions was analyzed in Ukraine. It was shown that CGMMV is an extremely rare pathogen in open-field conditions in our country. Duringthelast 8 years we have confirmed only several cases of CGMMV infection on cucumbers, squash, pumpkin and melon in Kyiv and Poltava regions. Additionally, half of these cases were mixed infection of 2–3 viruses. From 250 collected samples, CGMMV was detected in 5 plants totaling to only 2,4 %. Importantly, monoinfection of CGMMV was shown exclusively on cucumbers and watermelons, whereas melons and squashes were mixed infected. Melons were typically infected with CGMMV and Watermelon mosaic virus 2 (WMV 2) or with WMV 2 and Zucchini yellow mosaic virus (ZYMV). Squash plants were infected with CGMMV and Cucumber mosaic virus (CMV).Consequently, we have checked commercially available seeds of cucumber, squash, zucchini, melon and watermelon from different producers in Ukraine for virus contamination and have confirmed the occurrence of CGMMV in some seed batches. Conclusions: Long-term observations in Ukraine showed that Cucumber green mottle mosaic virus infected mostly cucumbers cultivated in greenhouses and was only rarely found in cucurbits grown in open-field conditions. Screening of commercially available seed material indicated that 16 % of it was contaminated with CGMMV. Basing on available data, it is presumed that virus-contaminated seed material may pose a major threat of CGMMV occurrence and spread on cucurbits cultivated in open-field conditions.

scholarly journals Inheritance of Resistance to the Watermelon Strain of Papaya Ringspot Virus in the Cucumber Line TMG-1

HortScience ◽  
1995 ◽  
Vol 30 (2) ◽  
pp. 338-340 ◽  
Author(s):  
T. Wai ◽  
R. Grumet
Keyword(s):  
Mosaic Virus ◽  
Dominant Gene ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Genetics Of Resistance ◽  
Elisa Data

The inbred cucumber (Cucumis sativus L.) line TMG-1 is resistant to three potyviruses: zucchini yellow mosaic virus (ZYMV), watermelon mosaic virus (WMV), and the watermelon strain of papaya ringspot virus (PRSV-W). In this study we sought to determine the genetics of resistance to PRSV-W. TMG-1 was crossed with WI-2757, an inbred line susceptible to all three viruses. Segregation data indicated that resistance to PRSV-W was due to a single dominant gene (proposed designation, Prsv-2). Enzyme-linked immunosorbent assay (ELISA) data suggested that the mechanism of resistance to PRSV-W differs from that for ZYMV and WMV, and may be better described as tolerance. Although the plants were free of symptoms, high PRSV-W titers existed in young expanding leaves of the TMG-1 plants and the WI-2757 × TMG-1 F1 progeny.

scholarly journals Virus Quantification, Flowering, Yield, and Fruit Quality in Tropical Pumpkin (Cucurbita moschata Duchesne) Genotypes Susceptible or Resistant to Two Potyviruses

HortScience ◽  
2020 ◽  
pp. 1-11
Author(s):  
Wilfredo Seda-Martínez ◽  
Linda Wessel-Beaver ◽  
Angela Linares-Ramírez ◽  
Jose Carlos V. Rodrigues
Keyword(s):  
Puerto Rico ◽  
Fruit Quality ◽  
Symptom Severity ◽  
Dry Matter ◽  
Fruit Weight ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cucurbita Moschata ◽  
Moderate Resistance ◽  
Moderately Resistant

Infecting cucurbits around the world, Papaya ringspot virus (PRSV) and Zucchini yellow mosaic virus (ZYMV) are members of the genus Potyvirus and family Potyviridae. Tropical pumpkin is grown globally in the lowland humid tropics. In Puerto Rico, tropical pumpkin is the second most important vegetable crop in economic value. In trials in Puerto Rico in 2016 and 2017, susceptible genotypes ‘Waltham’, Mos166, ‘Taína Dorada’ (2016 only), ‘Soler’ with moderate resistance to ZYMV, and resistant ‘Menina’ and ‘Nigerian Local’ were inoculated with PRSV and ZYMV and evaluated in the greenhouse and field. Mock-inoculated (buffer) controls were included. Puerto Rico strains of PRSV and ZYMV were originally collected from plants of Cucurbita moschata in Puerto Rico. Presence of virus was determined by Double Antibody Sandwich (DAS) enzyme-linked immunosorbent assay (ELISA) and symptom severity was evaluated on a 0 to 5 scale in both trials. Days to anthesis of first staminate and pistillate flower were recorded for each plot. Number of fruits, fruit weight per plant, average fruit weight, fruit and mesocarp diameter, mesocarp color, °Brix, and percentage dry matter were measured in 2017. ‘Waltham’, Mos166, ‘Taína Dorada’, and ‘Soler’ tested positive for PRSV when inoculated with PRSV and positive for ZYMV when inoculated with ZYMV. For both PRSV and ZYMV, symptom severity was less (severity = 0) in resistant genotypes ‘Menina’ and ‘Nigerian Local’ than in all other genotypes. ‘Soler’ inoculated with ZYMV exhibited less symptom severity than that of susceptible genotypes. The degree of symptom severity of ‘Soler’ inoculated with PRSV was similar to susceptible genotypes. Symptom severity in plants inoculated with ZYMV was generally greater than when inoculated with PRSV. Compared with controls, yield per plant was unaffected by inoculation with potyvirus in resistant cultivar ‘Menina’. Unexpectedly, yield in resistant ‘Nigerian Local’ was reduced an average of 45% over control plots. Yield loss was 100% in inoculated plots of susceptible ‘Waltham’. Yield reduction ranged from 35% to 80% for susceptible Mos166 and moderately resistant ‘Soler’. There was little evidence that days to anthesis, average fruit weight, fruit diameter, and fruit quality (mesocarp thickness, chroma, hue angle, °Brix and dry matter) of plants inoculated with virus were different from that of uninoculated control plants. The exception was moderately resistant ‘Soler’ where plants inoculated with ZYMV produced fruits with a 32% reduction in average weight, as well as reductions in diameter, mesocarp thickness, and color saturation (chroma) compared with controls. This was unexpected given that ‘Soler’ has some resistance to ZYMV. Greenhouse evaluations by ELISA or symptom severity were generally useful in predicting field resistance to PRSV and ZYMV. In summary, yield reductions of up to 100% can be expected in C. moschata genotypes susceptible to PRSV or ZYMV, but fruit quality traits are usually unaffected. Moderate resistance to ZYMV in ‘Soler’ was observed to reduced symptom severity but not negative effects on yield and other traits. ‘Soler’ was not resistant to PRSV. ‘Menina’ rather than ‘Nigerian Local’ appears to be the best source of resistance because yield of the former was not impacted by inoculation with either potyvirus.

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