scholarly journals Genetic Control of Seven Enzyme Systems in Ipomoea Species

1992 ◽  
Vol 117 (6) ◽  
pp. 1000-1005 ◽  
Author(s):  
Luz Marina Reyes ◽  
Wanda W. Collins
Keyword(s):  
Genetic Control ◽  
Gel Electrophoresis ◽  
Genetic Basis ◽  
Starch Gel Electrophoresis ◽  
Duplicated Genes ◽  
Shikimate Dehydrogenase ◽  
Glutamate Oxaloacetate Transaminase ◽  
Ipomoea Trifida ◽  
Polyploid Series ◽  
Starch Gel

Genetic control of seven enzymes in Ipomoea trifida (H.B.K.) G. Don. (diploid, tetraploid, and hexaploid populations) and I. batatas (L.) Lam. was studied by starch gel electrophoresis. Inter- and intraspecific polymorphisms were detected for all enzymes in the populations analyzed, except catalase (CAT, EC 1.11.1.6). Phosphoglucomutase (PGM; EC 2.7.5.1), phosphoglucose isomerase (PGI; EC 5.3.1.9), glutamate oxaloacetate transaminase (GOT; EC 2.6.1.1), menadione reductase (MNR; EC 1.6.99.2), shikimate dehydrogenase (SAD; EC 1.1.1.25), and malate dehydrogenase (MDH; EC 1.1.1.37) collectively were encoded by a minimum of 13 genetic loci resulting in 24 allozymes. Results from the diploid I. trifida were used to infer the genetic basis of these enzymes in the polyploid species. All polyploid populations shared almost the same number of allozymes with diploid I. trifida. PGM and PGI showed evidence of duplicated genes in the polyploid series. A unique allele for MNR was detected only in polyploid series.

scholarly journals Identifying Crabapple Cultivars by Isozymes

1995 ◽  
Vol 120 (5) ◽  
pp. 706-709 ◽  
Author(s):  
Robert D. Marquard ◽  
Charlotte R. Chan
Keyword(s):  
Alcohol Dehydrogenase ◽  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Enzyme System ◽  
Starch Gel Electrophoresis ◽  
Shikimate Dehydrogenase ◽  
Polymorphic Region ◽  
Banding Patterns ◽  
Phosphogluconate Dehydrogenase ◽  
Starch Gel

Forty-five crabapple (Malus spp.) cultivars were evaluated for 16 isozyme systems by starch gel electrophoresis. Of the 16 systems evaluated, 6 were useful in separating among cultivars. Enzyme systems used to distinguish among the cultivars included alcohol dehydrogenase, aspartate aminotransferase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucoisomerase, and shikimate dehydrogenase. Each enzyme system produced one well-resolved polymorphic region except for 6-phosphogluconate dehydrogenase, which produced two. Most crabapple selections could be identified when all six enzymes were evaluated. Alcohol dehydrogenase had the most diagnostic banding patterns useful for cultivar identification.

scholarly journals Inheritance of ADH, 6-PGDH, PGM, and SKDH in Allium fistulosum L.

1994 ◽  
Vol 119 (2) ◽  
pp. 335-338 ◽  
Author(s):  
Paul D. Mangum ◽  
Ellen B. Peffley
Keyword(s):  
Alcohol Dehydrogenase ◽  
Gel Electrophoresis ◽  
Allium Fistulosum ◽  
Starch Gel Electrophoresis ◽  
Shikimate Dehydrogenase ◽  
Chi Square ◽  
Phosphogluconate Dehydrogenase ◽  
Enzyme Systems ◽  
Isozyme Loci ◽  
Starch Gel

Horizontal starch gel electrophoresis was used to study the inheritance of isozyme phenotypes of four enzyme systems [alcohol dehydrogenase (ADH), 6-phosphogluconate dehydrogenase (6-PGDH), phosphoglucomutase (PGM), and shikimate dehydrogenase (SKDH)] in Allium fistulosum L. by monitoring segregations in backcross and F2 progeny. Segregation for most of the polymorphisms fit the expected Mendelian ratios as tested by the chi-square statistic. Three new isozyme loci were defined for onion. Two loci were found for 6-PGDH. Locus one was dimeric with two alleles, and locus two was monomorphic. SKDH was monomeric with two alleles.

scholarly journals Identification of Sweetpotato Cultivars Using Isozyme Analysis

HortScience ◽  
1991 ◽  
Vol 26 (3) ◽  
pp. 300-302 ◽  
Author(s):  
Larry S. Kennedy ◽  
Paul G. Thompson
Keyword(s):  
Gel Electrophoresis ◽  
Malic Enzyme ◽  
Ipomoea Batatas ◽  
Leaf Tissue ◽  
Xanthine Dehydrogenase ◽  
Starch Gel Electrophoresis ◽  
Glucosephosphate Isomerase ◽  
Shikimate Dehydrogenase ◽  
Phosphogluconate Dehydrogenase ◽  
Starch Gel

The enzymes alcohol dehydrogenase, diaphorase, esterase, glutamate dehydrogenase, glucosephosphate isomerase, isocitrate dehydrogenase, malate dehydrogenase, malic enzyme, 6-phosphogluconate dehydrogenase, phosphoglucomutase, shikimate dehydrogenase, and xanthine dehydrogenase were analyzed by starch gel electrophoresis of leaf tissue from nine sweetpotato [Ipomoea batatas (L.) Lam.] cultivars. Bands of most enzymes were well-defined. Polymorphisms were found in nine enzymes, and cultivars were identified by comparing polymorphisms.

scholarly journals Inheritance of Aconitase, Shikimate Dehydrogenase, and Phosphoglucose Isomerase in Guayule

1991 ◽  
Vol 116 (4) ◽  
pp. 737-739 ◽  
Author(s):  
A. Hashemi ◽  
A. Estilai ◽  
B. Ehdaie
Keyword(s):  
Gel Electrophoresis ◽  
Protein Structures ◽  
Phosphoglucose Isomerase ◽  
Starch Gel Electrophoresis ◽  
Self Incompatibility ◽  
Parthenium Argentatum ◽  
Shikimate Dehydrogenase ◽  
Starch Gel ◽  
Early Seedling ◽  
Monomeric Structure

Starch gel electrophoresis was carried out to investigate inheritance of aconitase (ACO), shikimate dehydrogenase (SKDH), and phosphoglucose isomerase (PGI) in guayule (Parthenium argentatum Gray). Self-incompatibility of diploid guayule (2n = 36), prevented production of F2 generations. A series of crosses was made for segregation analysis. The enzyme ACO showed two zones of activity—one monomorphic and the other polymorphic, representing one locus with two alleles. SKDH was found to be coded by two loci with three alleles at each locus. Four zones of activity were found for PGI. PGI-1, the fastest zone, was poorly resolved and appeared variable. Segregation data indicated that PGI-2 is monogenic and controlled by five codominant alleles. Poor resolution of PGI-4 made it impossible to determine whether PGI-3 was the product of a separate locus or resulted from intergenic cross dimerization between PGI-2 and PGI-4. The dimeric characteristic of PGI-2 and the monomeric structure of SKDH and ACO-1 in guayule agreed with the protein structures previously reported for these enzymes in other plant species. The isozyme variation of this investigation maybe used in a breeding program to identify sexual and maternal type progenies of facultative apomictic guayule plants at the early seedling stage.

scholarly journals PGI Isozyme Diversity and Its Genetic Control in Mango

HortScience ◽  
1992 ◽  
Vol 27 (3) ◽  
pp. 252-254 ◽  
Author(s):  
Chemda Degani ◽  
Menashe Cohen ◽  
Ruth El-Batsri ◽  
Shmuel Gazit
Keyword(s):  
Genetic Control ◽  
Gel Electrophoresis ◽  
Mangifera Indica ◽  
Phosphoglucose Isomerase ◽  
Starch Gel Electrophoresis ◽  
Banding Patterns ◽  
Starch Gel ◽  
Isozyme Diversity

Leaf phosphoglucose isomerase (PGI) isozymes from 139 cultivars and seedlings of mango (Mangifera indica L.) were analyzed by starch gel electrophoresis. Six distinct banding patterns of PGI-2 consisting of single- and triple-banded phenotypes were detected. The genetic control of PGI-2 isozymes were inferred from segregating progenies of self-pollinated parent cultivars having triple-banded phenotypes. Comparison of the banding patterns of PGI-2 isozymes extracted from the pollen and the leaf of the same heterozygous cultivar demonstrates the allelism of the Pgi-2 locus.

scholarly journals INHERITANCE OF ISOZYMES: DESCRIPTION OF NEW LOCI IN ONIONS ISOZYMES

HortScience ◽  
1993 ◽  
Vol 28 (4) ◽  
pp. 269D-269
Author(s):  
Paul D. Mangum ◽  
Ellen B. Peffley
Keyword(s):  
Allium Cepa ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Shikimate Dehydrogenase ◽  
Chi Square ◽  
Phosphogluconate Dehydrogenase ◽  
Enzyme Systems ◽  
Isozyme Loci ◽  
Starch Gel ◽  
Mode Of Inheritance

Horizontal starch gel electrophoresis was used to study the mode of inheritance of isozyme phenotypes of four enzyme systems (ADH, 6-PGDH, PGM, and SKDH) in Allium cepa L. and A. fistulosum L. by monitoring segregations in backcross and F2 progeny. Segregation for most of the polymorphisms fit the expected Mendelian ratios as tested by the chi-square statistic. Three new isozyme loci were defined for onion. 6-phosphogluconate dehydrogenase was dimeric in structure, with two alleles present at the first locus, while a second locus was monomorphic. Shikimate dehydrogenase was monomeric with two alleles.

Fractionation of Plasminogen by Starch Gel Electrophoresis

1964 ◽  
Vol 12 (01) ◽  
pp. 126-136 ◽  
Author(s):  
Karl H. Slotta ◽  
J. D Gonzalez
Keyword(s):  
Gel Electrophoresis ◽  
Room Temperature ◽  
Broad Band ◽  
Caproic Acid ◽  
Starch Gel Electrophoresis ◽  
Full Activity ◽  
Veronal Buffer ◽  
Starch Gel ◽  
Alkaline Range

SummaryWhen urea or ε-amino caproic acid were used as solublizing agents for plasminogen in electrophoretic experiments, only one broad band of the proenzyme was obtained on acetate cellulose, in starch block, and in acrylamide gel. In starch gel electrophoresis, however, both forms of plasminogen – the native or euglobulin and Kline’s or Pseudoglobulin plasminogen – separated into six bands. These migrated toward the cathode at room temperature in borate or veronal buffer in the alkaline range and showed full activity in fibrinagar-streptokinase plates.

scholarly journals THE INHERITANCE OF ENZYME VARIANTS FOR TYROSINE AMINOTRANSFERASE, NADP-DEPENDENT MALATE DEHYDROGENASE, NADP-DEPENDENT ISOCITRATE DEHYDROGENASE, AND TETRAZOLIUM OXIDASE IN TETRAHYMENA PYRIFORMIS, SYNGEN 1

Genetics ◽  
1973 ◽  
Vol 74 (4) ◽  
pp. 595-603
Author(s):  
D Borden ◽  
E T Miller ◽  
D L Nanney ◽  
G S Whitt
Keyword(s):  
Detailed Analysis ◽  
Malate Dehydrogenase ◽  
Isocitrate Dehydrogenase ◽  
Gel Electrophoresis ◽  
Tetrahymena Pyriformis ◽  
Tyrosine Aminotransferase ◽  
Breeding Program ◽  
Starch Gel Electrophoresis ◽  
Enzyme Locus ◽  
Starch Gel

ABSTRACT The isozymic patterns of tyrosine aminotransferase, NADP malate dehydrogenase, NADP isocitrate dehydrogenase, and tetrazolium oxidase were examined by starch-gel electrophoresis in Tetrahymena pyriformis, syngen 1. The genetics of the alleles controlling these enzymes was studied through a breeding program. Each enzyme locus was shown to assort vegetatively, as do other loci in this organism. A detailed analysis of the assortment process for the tyrosine aminotransferase locus indicated that the rate of stabilization of heterozygotes into pure types was essentially identical to previously-reported rates for other loci.

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