scholarly journals Randomly Amplified Polymorphic DNAs in the Cultivated Strawberry, Fragaria ×ananassa

1994 ◽  
Vol 119 (4) ◽  
pp. 862-864 ◽  
Author(s):  
J.F. Hancock ◽  
P.A. Callow ◽  
Douglas V. Shaw
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Map ◽  
Fragaria Ananassa ◽  
Chain Reaction ◽  
Banding Patterns ◽  
Amplification Profile ◽  
Polymerase Chain ◽  
Coefficient Of Coancestry ◽  
Dna Primers ◽  
Strawberry Cultivars

Eight strawberry cultivars or advanced selections from the Univ. of California, Davis, breeding program were screened for polymorphisms using the polymerase chain reaction (PCR) and 43 random 10-base DNA primers. Over 60% of the primers screened resulted in replicable polymorphic banding patterns (amplification profiles), and a subset of ten primers that exhibited high levels of amplification profile polymorphism was used to identify each of the eight genotypes uniquely. There was also a significant product-moment correlation (r = 0.64, P < 0.01) between number of shared amplification profile phenotypes and pairwise coefficient of coancestry. This technology shows high promise as a means of verifying the identity of cultivars and developing a genetic map of the octoploid cultivated strawberry.

Polymerase chain reaction based mapping of rye involving repeated DNA sequences

Genome ◽  
1992 ◽  
Vol 35 (4) ◽  
pp. 621-626 ◽  
Author(s):  
Peter M. Rogowsky ◽  
Ken W. Shepherd ◽  
Peter Langridge
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Chromosome 1 ◽  
Repeated Dna ◽  
Chain Reaction ◽  
Pcr Marker ◽  
Banding Patterns ◽  
Repeated Dna Sequences ◽  
Cereal Rye ◽  
Polymerase Chain

A novel type of polymerase chain reaction (PCR) marker was developed for the mapping of cereal rye (Secale cereale). Primer pairs were synthesized targeting the insertion sites of three individual copies of the R173 family of rye specific repeated DNA sequences. While one primer was derived from a sequence within the respective R173 element, the second primer corresponded to a flanking region. The complex banding patterns obtained in rye allowed not only the mapping of the three R173 elements to certain chromosome regions of 1RS (the short arm of rye chromosome 1) but also the mapping of an additional 3–10 easily identifiable bands per primer pair to other rye chromosomes. Linkage mapping of a polymorphic 1R band derived from three rye cultivars demonstrated the presence of nonallelic, dominant markers in two independent crosses. Because of the high copy number of the R173 family (15 000 copies per diploid rye genome), its dispersion over the entire length of all chromosomes and the high number of markers obtained per primer pair, PCR markers based on the R173 family provide an almost unlimited source for well-spaced markers in rye mapping.Key words: polymerase chain reaction, mapping, repetitive DNA sequences, wheat, rye.

Biofunctionalization of Polyoxometalates with DNA Primers, Their Use in the Polymerase Chain Reaction (PCR) and Electrochemical Detection of PCR Products

2015 ◽  
Vol 21 (49) ◽  
pp. 17721-17727 ◽  
Author(s):  
Ahmed M. Debela ◽  
Mayreli Ortiz ◽  
Valerio Beni ◽  
Serge Thorimbert ◽  
Denis Lesage ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Electrochemical Detection ◽  
Chain Reaction ◽  
Pcr Products ◽  
Polymerase Chain ◽  
Dna Primers

Detection of rye chromosome 2R using the polymerase chain reaction and sequence-specific DNA primers

Genome ◽  
1994 ◽  
Vol 37 (1) ◽  
pp. 19-22 ◽  
Author(s):  
J.-H. Lee ◽  
R. A. Graybosch ◽  
D. J. Lee
Keyword(s):  
Polymerase Chain Reaction ◽  
Wheat Chromosome ◽  
Specific Marker ◽  
Chain Reaction ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Pcr Product ◽  
Polymerase Chain ◽  
Wheat Lines ◽  
Dna Primers

Sequences derived from a rye gamma secalin gene were used as primers in polymerase chain reactions using DNA obtained from a series of wheat and triticale genetic stocks. A 473-bp fragment, the predicted size based on the distance between the selected primers, was found only in rye, triticales, and wheat lines carrying rye chromosome 2RS. Use of triticale lines with various wheat chromosome substitutions confirmed the chromosomal origin of the rye-specific marker. The presence of the 473-bp PCR product was always associated with the production of 75K secalins in grain samples. Thus, the primer sequences, and the clone of origin (pSC503), were both derived from the SEC-2 locus of rye chromosome 2RS.Key words: wheat (Triticum aestivum), rye (Secale cereale), chromosomal translocations, chromosomal substitutions, DNA polymerase chain reaction, sequence-specific primers.

Polymerase chain reaction banding patterns of the 5S rDNA gene as a diagnostic tool for the discrimination of South American mullets of the genus Mugil

2010 ◽  
Vol 42 (8) ◽  
pp. 1117-1122 ◽  
Author(s):  
Luis Fernando S Rodrigues-Filho ◽  
Divino Bruno da Cunha ◽  
Marcelo Vallinoto ◽  
Horacio Schneider ◽  
Iracilda Sampaio ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Diagnostic Tool ◽  
5S Rdna ◽  
South American ◽  
Chain Reaction ◽  
Banding Patterns ◽  
Rdna Gene ◽  
Polymerase Chain

scholarly journals Ichthyophonus sp. Infection in Opaleye (Girella nigricans)

2020 ◽  
Vol 57 (2) ◽  
pp. 316-320
Author(s):  
Elise E. B. LaDouceur ◽  
Judy St Leger ◽  
Alexandria Mena ◽  
Ashley Mackenzie ◽  
Jacob Gregg ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Adipose Tissue ◽  
Dna Sequence ◽  
Ribosomal Dna ◽  
Chain Reaction ◽  
Public Display ◽  
Freshwater Aquaculture ◽  
Polymerase Chain ◽  
Aquaculture Fish ◽  
Dna Primers

Over a 3-year-period, 17 wild-caught opaleye ( Girella nigricans) housed in a public display aquarium were found dead without premonitory signs. Grossly, 4 animals had pinpoint brown or black foci on coelomic adipose tissue. Histologically, liver, spleen, heart, and posterior kidney had mesomycetozoan granulomas in all cases; other organs were less commonly infected. Four opaleye had goiter; additional substantial lesions were not identified. Granulomas surrounded melanized debris, leukocytes, and mesomycetozoa represented by folded membranes (collapsed schizont walls), intact schizonts (50- to >200 µm in diameter with a multilaminate membrane), plasmodia (budding from schizonts or free in tissue), or rarely germinal tubes (budding from schizonts). Ichthyophonus was grown from fresh tissues in tissue explant broth cultures of the heart, liver, and/or spleen. Polymerase chain reaction using 18S ribosomal DNA primers amplified a 1730-bp region, and the DNA sequence was most similar to Ichthyophonus hoferi, which is often associated with freshwater aquaculture fish.

scholarly journals Genotyping of Serratia marcescens Strains Associated with Cucurbit Yellow Vine Disease by Repetitive Elements-Based Polymerase Chain Reaction and DNA-DNA Hybridization

2003 ◽  
Vol 93 (10) ◽  
pp. 1240-1246 ◽  
Author(s):  
Q. Zhang ◽  
R. Weyant ◽  
A. G. Steigerwalt ◽  
L. A. White ◽  
U. Melcher ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Serratia Marcescens ◽  
Dna Hybridization ◽  
Repetitive Elements ◽  
Ecological Niches ◽  
Significant Heterogeneity ◽  
Chain Reaction ◽  
Banding Patterns ◽  
Cucurbit Yellow Vine Disease ◽  
Polymerase Chain

The bacterium that causes cucurbit yellow vine disease (CYVD) has been placed in the species Serratia marcescens based on 16S rDNA and groE sequence analysis. However, phenotypic comparison of the organism with S. marcescens strains isolated from a variety of ecological niches showed significant heterogeneity. In this study, we compared the genomic DNA of S. marcescens strains from different niches as well as type strains of other Serratia spp. through repetitive elements-based polymerase chain reaction (rep-PCR) and DNA-DNA hybridization. With the former, CYVD strains showed identical banding patterns despite the fact that they were from different cucurbit hosts, geographic locations, and years of isolation. In the phylogenetic trees generated from rep-PCR banding patterns, CYVD strains clearly were differentiated from other strains but formed a loosely related group with S. marcescens strains from other niches. The homogeneity of CYVD strains was supported further by the DNA relatedness study, in that labeled DNA from the cantaloupe isolate, C01-A, showed an average relative binding ratio (RBR) of 99%, and 0.33% divergence to other CYVD strains. Used as a representative strain of CYVD, the labeled C01-A had a RBR of 76%, and a 4.5% divergence to the S. marcescens type strain. These data confirm the previous placement of CYVD strains in S. marcescens. Our investigations, including rep-PCR, DNA-DNA hybridization, and previous phenotyping experiments, have demonstrated that CYVD-associated strains of S. marcescens cluster together in a group significantly different from other strains of the species.

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