Erratum: Detection of rye chromosome 2R using the polymerase chain reaction and sequence-specific DNA primers

Sequences derived from a rye gamma secalin gene were used as primers in polymerase chain reactions using DNA obtained from a series of wheat and triticale genetic stocks. A 473-bp fragment, the predicted size based on the distance between the selected primers, was found only in rye, triticales, and wheat lines carrying rye chromosome 2RS. Use of triticale lines with various wheat chromosome substitutions confirmed the chromosomal origin of the rye-specific marker. The presence of the 473-bp PCR product was always associated with the production of 75K secalins in grain samples. Thus, the primer sequences, and the clone of origin (pSC503), were both derived from the SEC-2 locus of rye chromosome 2RS.Key words: wheat (Triticum aestivum), rye (Secale cereale), chromosomal translocations, chromosomal substitutions, DNA polymerase chain reaction, sequence-specific primers.

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Diagnostic markers for Planococcus ficus (Signoret) and Planococcus citri (Risso) by random amplification of polymorphic DNA-polymerase chain reaction and species-specific mitochondrial DNA primers

Journal of Applied Entomology ◽

10.1111/j.1439-0418.2006.01126.x ◽

2007 ◽

Vol 131 (1) ◽

pp. 59-64 ◽

Cited By ~ 21

Author(s):

M. A. Demontis ◽

S. Ortu ◽

A. Cocco ◽

A. Lentini ◽

Q. Migheli

Keyword(s):

Polymerase Chain Reaction ◽

Mitochondrial Dna ◽

Dna Polymerase ◽

Planococcus Citri ◽

Chain Reaction ◽

Planococcus Ficus ◽

Random Amplification ◽

Polymerase Chain ◽

Species Specific ◽

Dna Primers

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Randomly Amplified Polymorphic DNAs in the Cultivated Strawberry, Fragaria ×ananassa

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.119.4.862 ◽

1994 ◽

Vol 119 (4) ◽

pp. 862-864 ◽

Cited By ~ 14

Author(s):

J.F. Hancock ◽

P.A. Callow ◽

Douglas V. Shaw

Keyword(s):

Polymerase Chain Reaction ◽

Genetic Map ◽

Fragaria Ananassa ◽

Chain Reaction ◽

Banding Patterns ◽

Amplification Profile ◽

Polymerase Chain ◽

Coefficient Of Coancestry ◽

Dna Primers ◽

Strawberry Cultivars

Eight strawberry cultivars or advanced selections from the Univ. of California, Davis, breeding program were screened for polymorphisms using the polymerase chain reaction (PCR) and 43 random 10-base DNA primers. Over 60% of the primers screened resulted in replicable polymorphic banding patterns (amplification profiles), and a subset of ten primers that exhibited high levels of amplification profile polymorphism was used to identify each of the eight genotypes uniquely. There was also a significant product-moment correlation (r = 0.64, P < 0.01) between number of shared amplification profile phenotypes and pairwise coefficient of coancestry. This technology shows high promise as a means of verifying the identity of cultivars and developing a genetic map of the octoploid cultivated strawberry.

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Detection of genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of DNA primers

European Food Research and Technology ◽

10.1007/s00217-005-0066-2 ◽

2005 ◽

Vol 221 (6) ◽

pp. 725-730 ◽

Cited By ~ 5

Author(s):

Yi-Ching Liu ◽

Shin-Huang Lin ◽

Hsi-Mei Lai ◽

Shih-Tong Jeng

Keyword(s):

Polymerase Chain Reaction ◽

Genetically Modified ◽

Chain Reaction ◽

Genetically Modified Soybean ◽

Dual Pairs ◽

Polymerase Chain ◽

Dna Primers

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Ichthyophonus sp. Infection in Opaleye (Girella nigricans)

Veterinary Pathology ◽

10.1177/0300985819900015 ◽

2020 ◽

Vol 57 (2) ◽

pp. 316-320

Author(s):

Elise E. B. LaDouceur ◽

Judy St Leger ◽

Alexandria Mena ◽

Ashley Mackenzie ◽

Jacob Gregg ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Adipose Tissue ◽

Dna Sequence ◽

Ribosomal Dna ◽

Chain Reaction ◽

Public Display ◽

Freshwater Aquaculture ◽

Polymerase Chain ◽

Aquaculture Fish ◽

Dna Primers

Over a 3-year-period, 17 wild-caught opaleye ( Girella nigricans) housed in a public display aquarium were found dead without premonitory signs. Grossly, 4 animals had pinpoint brown or black foci on coelomic adipose tissue. Histologically, liver, spleen, heart, and posterior kidney had mesomycetozoan granulomas in all cases; other organs were less commonly infected. Four opaleye had goiter; additional substantial lesions were not identified. Granulomas surrounded melanized debris, leukocytes, and mesomycetozoa represented by folded membranes (collapsed schizont walls), intact schizonts (50- to >200 µm in diameter with a multilaminate membrane), plasmodia (budding from schizonts or free in tissue), or rarely germinal tubes (budding from schizonts). Ichthyophonus was grown from fresh tissues in tissue explant broth cultures of the heart, liver, and/or spleen. Polymerase chain reaction using 18S ribosomal DNA primers amplified a 1730-bp region, and the DNA sequence was most similar to Ichthyophonus hoferi, which is often associated with freshwater aquaculture fish.

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High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100166944 ◽

1996 ◽

Vol 54 ◽

pp. 896-897

Author(s):

G. W. Hacker ◽

I. Zehbe ◽

J. Hainfeld ◽

A.-H. Graf ◽

C. Hauser-Kronberger ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Messenger Rna ◽

High Performance ◽

Detection System ◽

Direct Methods ◽

Genetic Alterations ◽

Chain Reaction ◽

Protein Encoding ◽

Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

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