scholarly journals A DNA Marker for Columnar Growth Habit in Apple Contains a Simple Sequence Repeat

1997 ◽  
Vol 122 (3) ◽  
pp. 347-349 ◽  
Author(s):  
Minou Hemmat ◽  
Norman F. Weeden ◽  
Patrick J. Conner ◽  
Susan K. Brown
Keyword(s):  
Simple Sequence Repeat ◽  
Rapd Markers ◽  
Null Allele ◽  
Growth Habit ◽  
Bulked Segregant Analysis ◽  
Rapd Marker ◽  
Close Linkage ◽  
Sequence Repeat ◽  
Dna Fragment ◽  
Simple Sequence

The columnar mutation `Wijcik McIntosh' has attracted much attention because of its compact growth habit, which is compatible with high-density plantings. Using bulked segregant analysis, we identified several randomly amplified polymorphic DNA (RAPD) markers that displayed a close linkage with the columnar locus (Co). The RAPD marker that displayed the closest linkage was end sequenced to develop a sequence tagged site for rapidly screening segregating populations. A simple sequence repeat (SSR) of (GA)17 was identified within the DNA fragment. Four allelic forms, including an apparent null allele, could be distinguished among the cultivars tested. The null allele displayed close linkage with Co in two progenies, and we used this marker to identify the location of the gene on the apple linkage map.

scholarly journals ISSR and AFLP Markers Differ among American Watermelon Cultivars with Limited Genetic Diversity

2004 ◽  
Vol 129 (4) ◽  
pp. 553-558 ◽  
Author(s):  
Amnon Levi ◽  
Claude E. Thomas ◽  
M. Newman ◽  
O.U. K. Reddy ◽  
X. Zhang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Rapd Markers ◽  
Phenotypic Diversity ◽  
Citrullus Lanatus ◽  
Inter Simple Sequence Repeat ◽  
Aflp Markers ◽  
Sequence Repeat ◽  
Limited Genetic Diversity ◽  
Simple Sequence

Wide phenotypic diversity exists among American heirloom cultivars of watermelon (Citrullus lanatus var. lanatus). However, in published studies, low or no polymorphism was revealed among those heirlooms using isozyme or randomly amplified polymorphic DNA (RAPD) markers. In this study, experiments with inter-simple sequence repeat (ISSR) [also known as simple sequence repeat-(SSR-) anchored primers] and amplified fragment-length polymorphism (AFLP) markers produced high polymorphisms among watermelon heirloom cultivars. ISSR (111) and AFLP (118) markers (229 total) identified 80.2% to 97.8% genetic similarity among heirloom cultivars. The phylogenetic relations based on ISSR and AFLP markers are highly consistent with the parental records available for some of the heirloom cultivars, providing confidence in the dendogram constructed for heirlooms based on similarity values. As compared with RAPD markers, ISSRs and AFLPs are highly effective in differentiating among watermelon cultivars or elite lines with limited genetic diversity.

Molecular tagging of pod shattering tolerance trait in soybean[Glycine max (L.) Merrill] genotype MACS-450.

2017 ◽  
Author(s):  
Dilip S. Thakare ◽  
Vivek P. Chimote ◽  
Amit Adsul ◽  
Milind P. Deshmukh ◽  
Santosh C. Pulate
Keyword(s):  
Simple Sequence Repeat ◽  
Bulked Segregant Analysis ◽  
Susceptible Cultivar ◽  
Sequence Repeat ◽  
Molecular Tagging ◽  
Pod Shattering ◽  
Glycine Max L ◽  
Segregating Population ◽  
Simple Sequence ◽  
Tolerant Genotype

Pod shattering (Dehiscence) severely reduces seed yield in soybean. Molecular tagging of pod shattering resistance can help in the process of breeding for shattering tolerance. In this study, an segregating population of cross (susceptible cultivar Monetta x tolerant genotype MACS-450) was used for bulked segregant analysis (BSA) with 26 SSR (simple sequence repeat) primers known to amplify markers linked to 22 qPDH loci. Among them, eight polymorphic SSR markers, viz., Sat_350 (qPDH1-7), Satt185 (qPDH1-3), Satt674 (qPDH1-5 loci), Satt166 (qPDH3-5), SRM1 (qPDH1 loci) Sat_342 (qPDH3-2), Satt685 (qPDH1-2) and Sat_407 (qPDH3-1) were able to distinguish parents differing for pod shattering of them two primers Satt 166 and SRM1 yielded markers polymorphic in between shattering tolerant and susceptible bulks by amplifying only Satt166-200 bp marker and SRM1-234bp marker only in tolerant bulks. Only Satt166-200 bp marker was observed in tolerant parent, bulks and 14 plants; while another 237 bp marker for pod shattering susceptibility got amplified in 46 plants including 8 plants that were heterozygous for both alleles. SRM1-234 bp marker was observed only in tolerant parent, bulks and 15 plants; while another 237 bp marker for pod shattering susceptibility got amplified in 45 plants. In combined marker analysis, the markers Satt 166 (qPDH3-5 loci) and SRM1 (qPDH1 loci) were linked with pod shattering score and were also confirmed in individual 60 F2 segregants. Hence, these markers could be utilized in the marker assisted for pod shattering resistance/tolerance breeding of qPDH3-5 like and qPDH1 genes.

scholarly journals 050 Comparative Analysis of Cultivated Melon Groups (Cucumis melo L.) using Random Amplified Polymorphic DNA and Simple Sequence Repeat Markers

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 397B-397
Author(s):  
Jack E. Staub ◽  
Gennaro Fazio ◽  
Thomas Horejsi ◽  
Yael Danin-Poleg ◽  
Noa Reis ◽  
...  
Keyword(s):  
Simple Sequence Repeat ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Estimation Methods ◽  
Sequence Repeat ◽  
Ssr Primers ◽  
Nei's Distance ◽  
Highly Correlated ◽  
Simple Sequence

Random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers were used to characterize genetic relationships among 46 accessions in two C. melo L. subsp. melo (Cantalupensis, Inodorus) and subsp. agrestis (Conomon and Flexuosus) groups. Genetic distance (GD) estimates were made among and between accessions in four melon market classes [Galia, Ogen, Charentais, and Shipper (European and U.S. types)] of Cantalupensis, one market class of Inodorus (Cassaba and Honey Dew), one accession of Conomon, and one accession of Flexuosus by employing three GD estimators; simple matching coefficient, Jaccard's coefficient, and Nei's distance-D. Differences detected among 135 RAPD bands and 54 SSR bands (products of 17 SSR primers) were used to calculate GD. Band polymorphisms observed with 21 RAPD primers and 7 SSR primers was important in the detection of genetic differences. Estimators of GD were highly correlated (P > 0.0001; rs = 0.64 to 0.99) when comparisons were made between estimation methods within a particular marker system. Lower correlations (P > 0.001; rs = 0.17 to 0.40) were detected between marker systems using any one estimator. The GD of the Conomon and Flexuosus accessions was significantly different from the mean GD of all the market classes examined, and market classes were distinguishable from each other. Although lower coefficients of variation can be attained in the estimation of GD when using RAPDs compared to SSRs, the genetic relationships identified using these markers were generally similar. Results of RAPD marker analysis suggest that 80 marker bands were adequate for assessing the genetic variation present in the accessions examined.

Implementing molecular marker technology in forage improvement

2003 ◽  
pp. 229-238
Author(s):  
M. Faville ◽  
B. Barrett ◽  
A. Griffiths ◽  
M. Schreiber ◽  
C. Mercer ◽  
...  
Keyword(s):  
Quantitative Trait Locus ◽  
Genetic Variation ◽  
White Clover ◽  
Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Linkage Groups ◽  
Seedling Vigour ◽  
Genome Map ◽  
Trait Locus ◽  
Simple Sequence

Accelerated improvement of two cornerstones of New Zealand's pastoral industries, per ennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), may be realised through the application of markerassisted selection (MAS) strategies to enhance traditional plant breeding programmes. Genome maps constructed using molecular markers represent the enabling technology for such strategies and we have assembled maps for each species using EST-SSR markers - simple sequence repeat (SSR) markers developed from expressed sequence tags (ESTs) representing genes. A comprehensive map of the white clover genome has been completed, with 464 EST-SSR and genomic SSR marker loci spanning 1125 cM in total, distributed across 16 linkage groups. These have been further classified into eight pairs of linkage groups, representing contributions from the diploid progenitors of this tetraploid species. In perennial ryegrass a genome map based exclusively on EST-SSR loci was constructed, with 130 loci currently mapped to seven linkage groups and covering a distance of 391 cM. This map continues to be expanded with the addition of ESTSSR loci, and markers are being concurrently transferred to other populations segregating for economically significant traits. We have initiated gene discovery through quantitative trait locus (QTL) analysis in both species, and the efficacy of the white clover map for this purpose was demonstrated with the initial identification of multiple QTL controlling seed yield and seedling vigour. One QTL on linkage group D2 accounts for 25.9% of the genetic variation for seed yield, and a putative QTL accounting for 12.7% of the genetic variation for seedling vigour was detected on linkage group E1. The application of MAS to forage breeding based on recurrent selection is discussed. Keywords: genome map, marker-assisted selection, perennial ryegrass, QTL, quantitative trait locus, SSR, simple sequence repeat, white clover

Genetic diversity of Symplocos paniculata of Hunan province revealed by inter-simple sequence repeat (ISSR)

2019 ◽  
Vol 51 (5) ◽  
Author(s):  
Huifang Cao ◽  
Qiang Lin ◽  
Peiwang Li ◽  
Jingzhen Chen ◽  
Changzhu Li ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Hunan Province ◽  
Sequence Repeat ◽  
Simple Sequence

Mapping Unequal Crossing over Hotspot Region of Simple Sequence Repeat in Maize

2009 ◽  
Vol 35 (5) ◽  
pp. 958-961
Author(s):  
Ji-Hua TANG ◽  
Xi-Qing MA ◽  
Wen-Tao TENG ◽  
Jian-Bing YAN ◽  
Jing-Rui DAI ◽  
...  
Keyword(s):  
Simple Sequence Repeat ◽  
Crossing Over ◽  
Sequence Repeat ◽  
Unequal Crossing Over ◽  
Simple Sequence
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