scholarly journals Characterization of a Cucumber Mosaic Virus Isolate and Satellite RNA from the Ornamental Host Ajuga reptans `Royalty'

2003 ◽  
Vol 128 (2) ◽  
pp. 231-237 ◽  
Author(s):  
J.R. Fisher ◽  
S.G.P. Nameth
Keyword(s):  
Phaseolus Vulgaris ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Cucurbita Pepo ◽  
Virus Isolate ◽  
Aphis Gossypii ◽  
Satellite Rna ◽  
Ajuga Reptans ◽  
Black Beauty

Cucumber mosaic virus (CMV) was isolated from the perennial ornamental mint, Ajuga reptans L. `Royalty', using melon aphids (Aphis gossypii Glover). The isolate and its associated satellite RNA (satRNA) were biologically and chemically characterized. The satRNA was cloned and sequenced and is 338 nucleotides long and does not induce lethal necrosis on `Rutgers' tomato (Lycopersicon esculentum Mill.) or severe chlorosis on tobacco (Nicotiana L. spp.). The virus is ≈28 to 30 nm in diameter and reacts to CMV serological subgroup I antibodies. The virus is able to infect `Black Beauty' squash (Cucurbita pepo L.), cucumber (Cucumis sativus L.), and `Howden' pumpkin (Cucurbita pepo) but is not able to infect green bean (Phaseolus vulgaris L.) or cowpea [Vigna unguiculata (L.) Walp. ssp. unguiculata]. The virus is able to efficiently replicate its satRNA in tobacco and `Black Beauty' squash but replication is less efficient in cucumber, based on accumulation of double-stranded satRNA.

scholarly journals Characterization of a satellite RNA associated with strain K8 of cucumber mosaic virus

1990 ◽  
Vol 18 (15) ◽  
pp. 4593-4593 ◽  
Author(s):  
A. Fraile ◽  
E. Moriones ◽  
F. Garcia-Arenal
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Satellite Rna

scholarly journals Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops

2020 ◽  
Vol 38 (2) ◽  
pp. 218-225
Author(s):  
Diana Marcela Rivera-Toro ◽  
Juan Carlos Vaca-Vaca ◽  
Karina López-López
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Molecular Characterization ◽  
Aphis Gossypii ◽  
Chili Pepper ◽  
Specific Primers ◽  
Capsicum Spp ◽  
Valle Del Cauca ◽  
First Time

The chili pepper (Capsicum spp. L.) is a vegetable of economic importance that has been affected worldwide by the cucumber mosaic virus (CMV), a pathogen that causes a devastating disease in this crop. The aim of this research was the detection and characterization of CMV in chili pepper crops in Valle del Cauca, Colombia. Leaves of three chili pepper varieties (tabasco, cayenne and habanero) with viral symptoms were collected in four municipalities of Valle del Cauca. Total RNA was purified and a fragment of capsid protein (CP) from CMV was amplified by RT‑PCR. Then, it was sequenced and bioinformatically analyzed, and from these sequences, specific primers were designed. From 71 chili pepper samples collected in Palmira, Yumbo, Vijes and Yotoco, 37 were positive for CMV (52.1%). The CMV chili pepper sequence analysis showed that they had their highest identity (98.5%) with a CMV isolated from bananas in Ecuador. Specific primers designed for CMV chili pepper showed greater sensitivity for detecting this virus (64.7% vs. 52.1%). The CMV chili pepper CP analysis indicated that it could be transmitted by the species Aphis gossypii. This r the first time, the molecular characterization of CMV in three chili pepper varieties.

Characterization of a satellite RNA associated with cucumber mosaic virus

Virology ◽  
1978 ◽  
Vol 84 (2) ◽  
pp. 443-455 ◽  
Author(s):  
Allan R. Gould ◽  
Peter Palukaitis ◽  
Robert H. Symons ◽  
Donald W. Mossop
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Satellite Rna

Characterization of a bean rugose mosaic virus isolate and search for virus resistance in a germplasm bank of Phaseolus vulgaris

2020 ◽  
Vol 45 (1) ◽  
pp. 34-43
Author(s):  
Daniella Vieira Cândida ◽  
Geisiane Alves Rocha ◽  
Vanessa Duarte Dias ◽  
Marcos Gomes da Cunha ◽  
Josias Correa de Faria ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Mosaic Virus ◽  
Virus Resistance ◽  
Virus Isolate ◽  
Germplasm Bank ◽  
Rugose Mosaic

scholarly journals Characterization of the Interactions Between Cucumber mosaic virus and Potato virus Y in Mixed Infections in Tomato

2010 ◽  
Vol 23 (11) ◽  
pp. 1514-1524 ◽  
Author(s):  
Tiziana Mascia ◽  
Fabrizio Cillo ◽  
Vilma Fanelli ◽  
Mariella Matilde Finetti-Sialer ◽  
Angelo De Stradis ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Potato Virus ◽  
Mixed Infection ◽  
Potato Virus Y ◽  
Satellite Rna ◽  
Protein Coding ◽  
Disease Symptoms ◽  
2B Protein

Mixed infection with the SON41 strain of Potato virus Y (PVY-SON41) in tomato increased accumulation of RNAs of strains Fny and LS of Cucumber mosaic virus (CMV-Fny and CMV-LS, respectively) and enhanced disease symptoms. By contrast, replication of PVY-SON41 was downregulated by CMV-Fny and this was due to the CMV-Fny 2b protein. The CMV-FnyΔ2b mutant was unable to systemically invade the tomato plant because its movement was blocked at the bundle sheath of the phloem. The function needed for invading the phloem was complemented by PVY-SON41 in plants grown at 22°C whereas this complementation was not necessary in plants grown at 15°C. Mutations in the 2b protein coding sequence of CMV-Fny as well as inhibition of translation of the 2a/2b overlapping region of the 2a protein lessened both the accumulation of viral RNAs and the severity of symptoms. Both of these functions were complemented by PVY-SON41. Infection of CMV-Fny supporting replication of the Tfn-satellite RNA reduced the accumulation of CMV RNA and suppressed symptom expression also in plants mixed-infected with PVY-SON41. The interaction between CMV and PVY-SON41 in tomato exhibited different features from that documented in other hosts. The results of this work are relevant from an ecological and epidemiological perspective due to the frequency of natural mixed infection of CMV and PVY in tomato.

scholarly journals Ability of Aphis gossypii and Myzus persicae to Transmit Cucumber mosaic virus in Single and Mixed Infection with Two Potyviruses to Zucchini Squash

2008 ◽  
Vol 34 (2) ◽  
pp. 183-185 ◽  
Author(s):  
Zayame Vegette Pinto ◽  
Jorge Alberto Marques Rezende ◽  
Valdir Atsushi Yuki ◽  
Sônia Maria de Stefano Piedade
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Type ◽  
Mixed Infection ◽  
Myzus Persicae ◽  
Cucurbita Pepo ◽  
Aphis Gossypii ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus

The main objective of this work was to investigate the ability of Aphis gossypii and Myzus persicae to transmit Cucumber mosaic virus (CMV) singly and mixed with two potyviruses (Papaya ringspot virus - type W, PRSV-W and Zucchini yellow mosaic virus, ZYMV), to zucchini squash plants (Cucurbita pepo). The results showed that the potyviruses in general were more efficiently transmitted by both species of aphids as compared to CMV. The transmission of PRSV-W, ZYMV and CMV separately was more efficient than in mixture.

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