scholarly journals Ability of Aphis gossypii and Myzus persicae to Transmit Cucumber mosaic virus in Single and Mixed Infection with Two Potyviruses to Zucchini Squash

2008 ◽  
Vol 34 (2) ◽  
pp. 183-185 ◽  
Author(s):  
Zayame Vegette Pinto ◽  
Jorge Alberto Marques Rezende ◽  
Valdir Atsushi Yuki ◽  
Sônia Maria de Stefano Piedade
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Type ◽  
Mixed Infection ◽  
Myzus Persicae ◽  
Cucurbita Pepo ◽  
Aphis Gossypii ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus

The main objective of this work was to investigate the ability of Aphis gossypii and Myzus persicae to transmit Cucumber mosaic virus (CMV) singly and mixed with two potyviruses (Papaya ringspot virus - type W, PRSV-W and Zucchini yellow mosaic virus, ZYMV), to zucchini squash plants (Cucurbita pepo). The results showed that the potyviruses in general were more efficiently transmitted by both species of aphids as compared to CMV. The transmission of PRSV-W, ZYMV and CMV separately was more efficient than in mixture.

scholarly journals Danos na produção da abobrinha de moita causados pelo Papaya ringspot virus type W e Zucchini yellow mosaic virus

2007 ◽  
Vol 33 (2) ◽  
pp. 192-194 ◽  
Author(s):  
Mônica Juliani Zavaglia Pereira ◽  
Angelo Aparecido Barbosa Sussel ◽  
Ricardo Ferreira da Silva ◽  
Odair José Kuhn ◽  
Fernanda Domingues ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Type ◽  
Cucurbita Pepo ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus

Este trabalho teve por objetivo avaliar, em condições de casa de vegetação e de campo, os danos causados pelo PRSV-W e ZYMV em abobrinha-de-moita (Cucurbita pepo cv. Caserta). As plantas em casa de vegetação foram inoculadas com os vírus individualmente e em mistura aos 12 e 22 dias após emergência (DAE) e aos 5, 15 e 25 DAE no campo. Em casa de vegetação, as infecções com PRSV-W + ZYMV, PRSV-W e ZYMV, na primeira época de inoculação, ocasionaram reduções de área foliar de 39,6%, 36,8% e 12,1%, respectivamente. As massas fresca e seca também foram significativamente afetadas na primeira época de inoculação. No campo, as plantas com infecções individuais ou mistas dos potyvírus produziram frutos não comerciais em quantidades que variaram de 14 a 861 g/planta, dependendo da idade que foram inoculadas. As plantas tratadas com tampão fosfato aos 5, 15 e 25 DAE produziram em média 573 g, 937 g e 1172 g de frutos comerciais e 282 g, 221 g e 192 g de frutos não comerciais, respectivamente. A redução na massa fresca das plantas foi diretamente relacionada com a época de inoculação, com médias de 60,7% para aquelas inoculadas aos 5 DAE e de 22,7% para aquelas inoculadas aos 15 DAE. Na terceira época de inoculação não houve diferença significativa de massa fresca entre os tratamentos.

scholarly journals Yield of varieties of Cucurbita pepo preimmunized with mild strains of Papaya ringspot virus - type W and Zucchini yellow mosaic virus

2009 ◽  
Vol 66 (3) ◽  
pp. 419-424 ◽  
Author(s):  
Estela Bonilha ◽  
Ricardo Gioria ◽  
Rômulo Fujito Kobori ◽  
Paulo Tarcísio Della Vecchia ◽  
Sônia Maria de Stefano Piedade ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Type ◽  
Cucurbita Pepo ◽  
Zucchini Yellow Mosaic Virus ◽  
Fruit Weight ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Leaf Symptoms ◽  
Severe Leaf

Papaya ringspot virus - type W (PRSV-W) and Zucchini yellow mosaic virus (ZYMV) are the most prevalent viruses in cucurbit crops in Brazil and responsible for frequent yield losses. Diseases caused by these viruses are difficult to control. The objective of this work was to evaluate the effects of mild strains PRSV-W-1 and ZYMV-M on the yield of Cucurbita pepo L. cvs. Samira, Novita Plus, AF 2847, and Yasmin, under plastic greenhouse and field conditions. Plants infected with ZYMV-M and grown in a plastic greenhouse did not exhibit typical leaf symptoms or significant alterations in quantitative and qualitative fruit yield. However, when infected with PRSV-W-1, or PRSV-W-1 + ZYMV-M, the plants exhibited severe leaf mosaic symptoms and reduced fruit quality, although there were no changes in the number and mean fruit weight harvested from these plants. When these plants were infected with PRSV-W-1 and studied simultaneously in the field and plastic greenhouse, intensification of symptoms in the fruits and leaves was more pronounced under the greenhouse conditions. Quantitative yield did not change. Environmental factors seem to influence symptoms induced by PRSV-W-1.

scholarly journals Danos causados pelo Zucchini lethal chlorosis virus (ZLCV) sobre a produção de frutos comerciais de abobrinha de moita 'Caserta'

2009 ◽  
Vol 35 (3) ◽  
pp. 223-225 ◽  
Author(s):  
José Segundo Giampan ◽  
Jorge Alberto Marques Rezende ◽  
Sônia Maria De Stefano Piedade
Keyword(s):  
Mosaic Virus ◽  
Virus Type ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus

O ZLCV é um tospovírus encontrado com freqüência causando severos danos em cucurbitáceas. Nesse trabalho avaliaram-se os danos causados pelo ZLCV em abobrinha de moita 'Caserta', em campo na ESALQ/USP, Piracicaba-SP, onde esse vírus é freqüente. Plantas obtidas pela semeadura direta foram monitoradas periodicamente quanto à infecção pelo ZLCV por meio dos sintomas e por PTA-ELISA. Monitorou-se ainda a contaminação com Papaya ringspot virus - type W e Zucchini yellow mosaic virus, desconsiderando a produção dessas plantas. As plantas foram agrupadas em função da época de aparecimento dos sintomas do ZLCV, avaliando a produção de frutos comerciais (FC) e não comerciais (FNC) de cada grupo e comparando com a de plantas que permaneceram sem sintomas até o final do experimento. As plantas que apresentaram sintomas até os 23 dias após a emergência (DAE) não produziram qualquer tipo de frutos. FC foram colhidos de plantas que apresentaram sintomas a partir dos 42 DAE. Mesmo assim, houve redução de 78,5 % na produção de FC. Plantas que mostraram sintomas por ocasião da última colheita (55 DAE) apresentaram redução na produção de FC de 9,6 %. A infecção com o ZLCV até o início da frutificação inviabiliza a produção de FC de abobrinha de moita 'Caserta'.

scholarly journals First detection of papaya ringspot virus-type W and zucchini yellow mosaic virus infecting Cucurbita maxima in Paraguay

2019 ◽  
Vol 102 (1) ◽  
pp. 231-231
Author(s):  
Arnaldo Esquivel-Fariña ◽  
Viviana Marcela Camelo-García ◽  
Jorge Alberto Marques Rezende ◽  
Elliot Watanabe Kitajima ◽  
Luis Roberto González-Segnana
Keyword(s):  
Mosaic Virus ◽  
Virus Type ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Cucurbita Maxima

scholarly journals Identificação sorológica de espécies de vírus que infetam cucurbitáceas em áreas produtoras do Maranhão

2001 ◽  
Vol 26 (1) ◽  
pp. 90-92 ◽  
Author(s):  
MARIA C. C. L. MOURA ◽  
J. ALBERSIO A. LIMA ◽  
VANÚZIA B. OLIVEIRA ◽  
M. FÁTIMA B. GONÇALVES
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Cucurbita Moschata

Os vírus representam sérios obstáculos para o sucesso da olericultura no mundo inteiro, constituindo a identificação daqueles de maior incidência numa região, papel fundamental para o estabelecimento de estratégias de controle. Visitas de campo foram realizadas a plantios de espécies de cucurbitáceas em áreas produtoras do Maranhão e amostras foliares foram coletadas de 118 plantas com sintomas ou suspeita de sintomas de vírus, sendo 46 de abóbora (Cucurbita moschata), 30 de melancia (Citrullus lanatus), 23 de maxixe (Cucumis anguria), 13 de pepino (C. sativus) e seis de melão (C. melo). Todas as amostras foram testadas contra anti-soros específicos para os principais vírus das famílias Bromoviridae, Comoviridae e Potyviridae que infetam cucurbitáceas no Nordeste, mediante "enzyme-linked immunosorbent assay" (ELISA) indireto e dupla difusão em agar. Os resultados revelaram a identificação sorológica de Papaya ringspot vírus (PRSV) em 64,4% das amostras analisadas, seguido de Watermelon mosaic virus-2 (WMV-2) em 15,2%, Cucumber mosaic virus (CMV) em 6,8%, Squash mosaic virus (SqMV) em 3,4% e Zucchini yellow mosaic virus (ZYMV) em 3,4%. Este levantamento confirma a predominância do PRSV em espécies de cucurbitáceas cultivadas no estado do Maranhão.

Generation of transgenic watermelon resistant to Zucchini yellow mosaic virus and Papaya ringspot virus type W

2010 ◽  
Vol 30 (3) ◽  
pp. 359-371 ◽  
Author(s):  
Tsong-Ann Yu ◽  
Chu-Hui Chiang ◽  
Hui-Wen Wu ◽  
Chin-Mei Li ◽  
Ching-Fu Yang ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Type ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Transgenic Watermelon

scholarly journals Characterization of Synergy Between Cucumber mosaic virus and Potyviruses in Cucurbit Hosts

2002 ◽  
Vol 92 (1) ◽  
pp. 51-58 ◽  
Author(s):  
Yongzeng Wang ◽  
Victor Gaba ◽  
Jie Yang ◽  
Peter Palukaitis ◽  
Amit Gal-On
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Mixed Infection ◽  
Zucchini Yellow Mosaic Virus ◽  
Helper Virus ◽  
Synergistic Interaction ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Satellite Rnas ◽  
Rna Levels

Mixed infections of cucurbits by Cucumber mosaic virus (CMV) and potyviruses exhibit a synergistic interaction. Zucchini squash and melon plants coinfected by the potyvirus Zucchini yellow mosaic virus (ZYMV) and either Fny-CMV (subgroup IA) or LS-CMV (subgroup II) displayed strong synergistic pathological responses, eventually progressing to vascular wilt and plant death. Accumulation of Fny- or LS-CMV RNAs in a mixed infection with ZYMV in zucchini squash was slightly higher than infection with CMV strains alone. There was an increase in CMV (+) strand RNA levels, but no increase in CMV (-) RNA3 levels during mixed infection with ZYMV. Moreover, only the level of capsid protein from LS-CMV increased in mixed infection. ZYMV accumulated to similar levels in singly and mixed infected zucchini squash and melon plants. Coinfection of squash with the potyvirus Watermelon mosaic virus (WMV) and CMV strains increased both the Fny-CMV RNA levels and the LS-CMV RNA levels. However, CMV (-) strand RNA3 levels were increased little or not at all for CMV on coinfection with WMV. Infection of CMV strains (LS and Fny) containing satellite RNAs (WL47-sat RNA and B5*-sat RNA) reduced the accumulation of the helper virus RNA, except when B5*-sat RNA was mixed with LS- CMV. However, mixed infection containing ZYMV and the CMV strains with satellites reversed the suppression effect of satellite RNAs on helper virus accumulation and increased satellite RNA accumulation. The synergistic interaction between CMV and potyviruses in cucurbits exhibited different features from that documented in tobacco, indicating there are differences in the mechanisms of potyvirus synergistic phenomena.

scholarly journals Systemic movement of a movement-deficient strain of Cucumber mosaic virus in zucchini squash is facilitated by a cucurbit-infecting potyvirus

2002 ◽  
Vol 83 (12) ◽  
pp. 3173-3178 ◽  
Author(s):  
Seung Kook Choi ◽  
Ju Yeon Yoon ◽  
Ki Hyun Ryu ◽  
Jang Kyung Choi ◽  
Peter Palukaitis ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Cucurbita Pepo ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Long Distance ◽  
Systemic Movement ◽  
Tissue Print ◽  
Long Distance Movement

Zucchini squash (Cucurbita pepo) is a systemic host for most strains of the cucumovirus Cucumber mosaic virus (CMV), although the long-distance movement of the M strain of CMV (M-CMV) is inhibited in some cultivars. However, co-infection of zucchini plants with M-CMV and the potyvirus Zucchini yellow mosaic virus strain A (ZYMV-A) allowed M-CMV to move systemically, as demonstrated by tissue-print analysis. These doubly infected plants exhibited severe synergism in pathology. Infection of zucchini squash by M-CMV and an attenuated strain of ZYMV (ZYMV-AG) showed a milder synergy in pathology, in which ZYMV-AG also facilitated the long-distance movement of M-CMV similar to that promoted by ZYMV-A. Variation in the extent of synergy in pathology by the two strains of ZYMV did not correlate with differences in levels of accumulation of either virus. Thus, the extent of synergy in pathology is at least in part independent of the resistance-neutralizing function of the potyvirus.

scholarly journals Characterization of cucumber mosaic virus originating from cucurbits in Serbia

2011 ◽  
Vol 26 (4) ◽  
pp. 325-336 ◽  
Author(s):  
Ana Vucurovic ◽  
Aleksandra Bulajic ◽  
Ivana Stankovic ◽  
Danijela Ristic ◽  
Janos Berenji ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Mixed Infection ◽  
Zucchini Yellow Mosaic Virus ◽  
Amino Acid Identity ◽  
Plant Viruses ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Worldwide Distribution ◽  
Frequency Changes

Cucumber mosaic virus (CMV) is considered one of the most economically important plant viruses and has a worldwide distribution and a very wide host range including plants from family Cucurbitaceae. In Serbia, on cucurbits CMV was detected in single and mixed infections with Zucchini yellow mosaic virus (ZYMV) and Watermelon mosaic virus (WMV). Viruses, including CMV, are constantly present in cucurbit crops, but their frequency changes by year and locality. Surveys and sample collections were conducted in cucurbit crops in the period from 2008 to 2009 at 15 localities in Vojvodina province, and sample testing was carried out using the DAS-ELISA method and commercially available antisera for six economically most important cucurbit viruses. In 2008, a total of 51 samples were collected from 13 cucurbit crops of oilseed pumpkin Olinka variety, squash, and bottle gourd and CMV was detected in a total of 55% of tested samples with symptoms of viral infection. The most common infectious type was mixed infection with ZYMV and WMV (35.3%), and then mixed infection with ZYMV (17.7%) and WMV (2%). A total of 599 symptomatic samples of oilseed pumpkin Olinka variety, zucchini squash varieties Beogradska and Tosca, squash, and winter squash were collected in 15 cucurbits crops in 2009. CMV was present in 4.4% of total collected samples, in single infections in 1.3%, and in mixed with WMV or ZYMV in 1.3%, and 1.8%. Five CMV isolates were obtained by mechanical inoculations of N. glutinosa and one of them was selected for further biological characterization. Test plants which were described to be hosts of CMV expressed symptoms characteristic for those caused by CMV after inoculations by isolate 115-08. CMV specific primers Au1u/Au2d were used to amplify an 850 bp fragment using RT-PCR method. Amplified fragment encodes the entire viral coat protein (CP) gene and partial 5? and 3? UTRs of two selected CMV isolates. Amplified fragments were sequenced and deposited in the NCBI, where they were assigned accession numbers, HM065510 (115-08) and HM065509 (151-08). The sequences of CMV isolates from Serbia shared the highest nucleotide and amino acid identity with isolates from subgroup IA, from 99.5 to 97.4% and 99.1 to 97.4%, and the lowest identities were with the subgroup II isolates from 66.9 to 64, 5%, from 75.8 to 74.1%.

scholarly journals Identificação sorológica de espécies de potyvirus em melancia, no estado de Roraima

2004 ◽  
Vol 29 (6) ◽  
pp. 687-689 ◽  
Author(s):  
Bernardo A. Halfeld-Vieira ◽  
Najara F. Ramos ◽  
Francisco A.C. Rabelo Filho ◽  
M. Fátima B. Gonçalves ◽  
Katia L. Nechet ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus

No período de maio de 2003 a março de 2004, foram coletadas amostras foliares de plantas de melancia (Citrullus lanatus) de 21 campos de cultivo de cucurbitáceas, no Estado de Roraima. As amostras exibiam diferentes sintomas de vírus e foram levadas para o Laboratório de Virologia Vegetal da Universidade Federal do Ceará para serem testadas por "enzyme linked immunosorbent assay" (Elisa)-indireto, contra anti-soros específicos para Cucumber mosaic virus (CMV), Papaya ringspot virus estirpe melancia (PRSV-W), Watermelon mosaic virus (WMV) e Zucchini yellow mosaic virus (ZYMV). Nos testes de Elisa, utilizou-se o conjugado universal, anti-imunoglobulina (IgG) de coelho produzida em cabra conjugada à enzima fosfatase alcalina. Todas as amostras foram testadas, também, por dupla difusão contra o anti-soro para Squash mosaic virus (SqMV). Os resultados indicaram a presença do PRSV-W em 84,2% das amostras coletadas em maio de 2003, em 7,1% das amostras coletadas em dezembro de 2003 e em 55,6% das amostras coletadas em março de 2004. A presença do ZYMV foi observada em 10,5% das amostras coletadas em maio de 2003, 21,4% das amostras coletadas em dezembro de 2003 e em 25,9% das amostras de março de 2004. O WMV foi detectado somente em oito das amostras coletadas em março de 2004 (29,6%). Os resultados desta pesquisa confirmam a ampla dispersão do PRSV-W em cultivos de cucurbitáceas no território brasileiro e a preocupante expansão do ZYMV em razão dos elevados prejuízos que o mesmo tem causado em outras partes do mundo.

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