Effect of Different Concentration of Commercial Seaweed Liquid Extract of Ascophylum nodosum on Germination of Onion (Allium cepa L.)

AbstractBackgroundAllium species extracts including Allium cepa L. contain sulfur compounds, known for their antiplatelet, antimicrobial, antineoplasic activities.MethodologyAntibacterial activity of a formulation based on A. cepa extracts-liquid and lyophilized samples, has been demonstrated using two classes of bacteria: Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) and three methods: discs soaked with liquid extract, the wells method in the culture medium, filled with the liquid extract and lyophilized formulation extracts transformed by the incorporation of ultrapure water. The second part of this study includes identification of thiosulfinates compounds from the studied samples by high performance liquid chromatography - mass spectrometry (HPLC-MS).ResultsThe most important inhibition and the highest antibacterial effficiency were observed against Gram-positive bacteria, such as Staphylococcus aureus. The HPLC-MS thiosulfinates profile of the tested formulation extracts shows the presence of seven thiosulfinate compounds, MeS(O)S 1-propenyl (E,Z); n-PrS(O)S 1-propenyl-(E); n-PrS(O)S 1-propenyl-(Z); trans-zwiebelane; n-PrS(O)CHEtSS-1propenyl; 1-propenylS(O)CHEtSS1-propenyl, which may be responsible for antibacterial activity.ConclusionTesting antimicrobial effects using the three mentioned methods confirmed the antimicrobial activity of the tested samples based on A. cepa extracts, with a demonstrated content of seven thiosulfinate compounds.

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PEMBUATAN LULUR KRIM DARI LIMBAH KULIT BAWANG MERAH (Allium cepa L) DAN CANGKANG TELUR AYAM DENGAN EMULGATOR SPAN-TWEEN 80

Jurnal Insan Farmasi Indonesia ◽

10.36387/jifi.v3i2.579 ◽

2020 ◽

Vol 3 (2) ◽

pp. 343-353

Author(s):

Rina Kurniaty ◽

Keyword(s):

Allium Cepa ◽

Tween 80 ◽

Ph Measurement ◽

Homogeneity Test ◽

Onion Extract ◽

Liquid Extract ◽

Allium Cepa L ◽

Type Test ◽

Chicken Eggshell ◽

Dispersion Test

Cream scrub is a body scrub that contains slightly harsh ingredients with cream-based ingredients. In this study, onion extract (Allium cepa L.) and chicken eggshell powder were used in order to determine which emulgator concentration (Span-Tween 80) could provide the best stability of cream scrub. Onion extract was made by maceration using methanol solvent to obtain liquid extract, afterward concentrated using vacuum rotary evaporator until a thick extract was obtained. There were 3 concentration variation of emulgator used for each 100 g of cream scrub formulation, namely 1% (F1), 2% (F2), and 3% (F3). Evaluation of cream scrub included organoleptic test, homogeneity test, dispersion test, emulsion type test and pH measurement. The results showed that all three cream scrub formulations were homogeneous and the cream scrub had M/A emulsion type. The results of dispersion test showed that F2 cream scrub had greater dispersibility than F1 and F3. Emulgator span-tween 80 with a concentration of 2% provided the best stability for cream scrub formulations.

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