Antioxidants Activity of Self-Nanoemulsifying Drug Delivery System on Dayak Onions Extract (Eleutherine palmifolia) using DPPH (2,2 difenil-1- picrylhydrazyl) Method

Dayak onions (Eleutherine palmifolia L. Merr) is one plant that has been proven to have benefits as an antioxidant. The Dayak extract is formulated in the self nano emulsifying drug delivery system (SNEDDS) because the extract has low water solubility. The aims this study to develop the SNEDDS formulation system by testing its antioxidant activity. We determined whether there was an increase in antioxidant activity when formulated in the form of SNEDDS or not. The results were then compared with a solution of Dayak onions extract without using SNEDDS. The obtained formula was the optimal result that has been done before using the D-optimal mixture design method. The results of the components consisted of 50 mg of Dayak extract, 10 % caprylic triglyceride as oil, 1% tween 80, and 6,60 % transcutol as a combination surfactant and 12,40 % propylene glycol as co-surfactant. Antioxidant activity testing was carried out using the DPPH (1,1-Diphenyl-2-picrylhydrazyl) method. The antioxidant test using the DPPH method was done with two samples, namely the extract solution and Dayak onion extract SNEDDS. We elaborated the research by using UV-VIS spectrophotometer. Each sample was made into five concentrations, namely 30 ppm, 60 ppm, 90 ppm, 120 ppm, and 150 ppm, and carried out three times replications. The results showed that the IC50 value in the Dayak onion extract solution was 227,19 ppm (very low), while for the SNEDDS solution for the Dayak onion extract the IC50 value obtained was 38,97 ppm (very strong). The analysis was carried out next using an independent T-Test to obtain the results. There was no significant difference between the extract solution and the SNEDDS solution of Dayak onion extract.

Pengaruh Pemberian Ekstrak Bawang Merah dan Kecambah dengan Pemberian Pupuk Cair Hayati terhadap Pertumbuhan Seedling Manggis (Garcinia mangostana L.)

Mangosteen (Garcinia mangostana L) seeds from seed, or commonly called seedling, have relatively few lateral roots and are poor in root hairs so that their growth is slow (having a long juvenile period of up to 10-15 years). For this reason, it is necessary to make efforts so that mangosteen seedlings can have a good root system through the addition of natural growth regulators. The purpose of this study was to determine the effect of red onion extract, a mixture of onion extract and mung bean sprouts combined with biological liquid fertilizer on the growth of mangosteen seedling. This research was carried out in the Horticulture FP greenhouse in March 2020–July 2020. This study was arranged in a factorial (3 x 2) in a Randomized Block Design (RAK) with three replications. The first factor was natural ZPT treatment: without extract, red onion extract 400 g/L, and mixed extract of onion 200 g/L and green bean sprouts 200 g/L. The second factor is the provision of biological fertilizers, namely without biological fertilizers and 15 ml/L). The results showed that the administration of mixed extracts of shallots and mung bean sprouts tended to have better growth which could be seen from the highest average value for the variable plant height 6.04 cm, stem diameter 2.93 mm, and the number of secondary roots. The mixed extract treatment of shallots and mung bean sprouts also increased the widest leaf area by a difference of 3.5 cm² compared to the control. Keywords : extract of shallot/ green bean sprout, growth regulator, mangosteen

PENGARUH KONSENTRASI EKSTRAK BAWANG MERAH DAN LAMA PERENDAMAN STEK JAMBU AIR MADU VARIETAS DELI HIJAU (Syzygium aqueum L.)

This study was conducted to determine the effects of interaction and the main response of the onion extract concentration and soaking time on cuttings guava water honey of green Deli variety (Syzygium aqueum L.). A completely randomized design (CRD) factorial was used in this study, consisting of 2 factors. The first factor was the concentration of onion extract consisting of 4 levels, i.e control, 100 g, 300 g, and 500 g of onion extract per 100 ml water. The second factor was the soaking time which consisted of 4 levels, i.e the soaking time of cuttings 3 hours, 6 hours, 9 hours, and 12 hours. The parameters observed were percentage of cuttings grown, age of germination days after planting, number of shoots, number of roots, longest root length, root volume. Observational data were analyzed statistically and continued with Tukey's HSD tests (p< 0.05). The results showed the interaction of onion extract concentration and soaking time had significant effects on the parameters for the percentage of cuttings grown, sprout age, number of shoots, number of roots, longest root length, and root volume. The best treatment was the combination of onion extract 300 g per 100 ml of water and soaking time on 9 hours.

Pengaruh Kombinasi Polimer Polivinil Pirolidon dan Etil Selulosa terhadap Karakteristik dan Uji Penetrasi Formulasi Transdermal Patch Ekstrak Bawang Dayak (Eleutherine palmifolia (L)

The transdermal patch can deliver the active substance with good bioavailability, then made formulations of such preparations from dayak onion extract, which has a compound content of flavonoids, with a combination of polymer PVP K30 and ethyl cellulose to produce transdermal patches with good physical evaluation and penetration. The research aims to carry out the effect and ideal formulation of transdermal patches of dayak onion extract with a combination of polymers PVP K30 and ethyl cellulose based on physical evaluation and penetration—manufacture of transdermal patches using the solvent evaporation method. Physical evaluation includes organoleptic testing, weight uniformity, thickness, folding resistance, and moisture testing. Then evaluate the penetration of the active substance using Franz diffusion cells. Analyze data with One Way ANOVA. The physical evaluation results of weight uniformity, patch thickness, folding resistance, and moisture test of transdermal patches on F1, F2, F3, and F4 meet the requirements. As for the results of penetration tests, F1, F2, F3, and F4 can be penetrated from 120 minutes to 180 minutes with the concentration of active substances that are linearly penetrated. The combination of polymers PVP K30 and ethylcellulose has affected the physical evaluation of transdermal patches of dayak onion extract. Based on the physical evaluation and penetration test in vitro obtained, the most optimal formula results are F3 with comparison PVP K30 and ethyl cellulose (100:300).

Uji Efek Ekstrak Bawang Dayak (Eleutherine Americana Merr) Terhadap Motilitas Progresif 3 Spermatozoa

<p><strong>Abstract</strong></p><p><strong> </strong></p><p><strong>Background:</strong> <em>Dayak</em> onion extract can be used as antioxidants in reducing lead toxicity. This study aims to prove the potential of <em>dayak</em> onion in inhibiting decrease of motility testicular spermatozoa of mice induced by lead acetate.</p><p><strong>Method:</strong> This study was a laboratory experimental study using Posttest Only Control Group Design design. The experimental animals were 30 mice (Mus musculus), divided into 5 groups. The K0 group was a negative control group receiving a Na-CMC 0,5% for 38 days at 0.1 ml. Day 1-3 K1 positive control group received Na-CMC 0,5%, K2-K4 group received <em>dayak</em> onion extract, K2 of 30 mg/kgBW, K3 60 mg/kgBW, and K4 120 mg/kgBW dose. Day 4-38 K1 group received 0.1 ml lead acetate, K2-K4 received lead acetate of 0.1 ml + <em>dayak</em> onion extract, K2 of 30mg/kgBW, K3 60 mg/kgBW, and K4 120 mg/kgBW as much as 0.2 ml.</p><p><strong>Result:</strong> There is a significant differences in spermatozoa concentration between K0 group with K3, K1 with K3 and K4, K2 with K3. Spermatozoa motility progressive 3 between K0 with K1, K2, K3, K4, K1 with K2, K3, K4 showed significant differences, while Testicular MDA content between K0 with K1, K2, K3 and K4, K1 with K0 and K3 showed significant differences.</p><p><strong>Conclusion</strong><strong>:</strong> <em>Dayak</em> onion extract inhibit the decrease in spermatozoa concentration, inhibit the decrease in spermatozoa motility, and decrease testicular MDA level in mice induced with lead acetate.</p><p> </p><p> </p><p><strong>Keyword</strong> : lead acetate, <em>dayak</em> onion extract, spermatozoa motility</p>