A SIMPLE VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF SITAGLIPTIN PHOSPHATE IN BULK AND PHARMACEUTICAL TABLET DOSAGE FORM

New first derivative ratio spectrophotometric method was developed for simultaneous estimation of telmisartan hydrochloride (TELM) and hydrochlorothiazide (HCTZ) in combined tablet dosage form without prior separation. The amplitude in the first derivative of the corresponding ratio spectra at 263.2 nm and 278.4 nm of TELM and for HCTZ at 287.2 nm and 304.8 nm were selected for the determination. The calibration graphs were established in the range of 1-10μg/mL and 2-15 μg/mL of TELM and HCTZ, respectively. The percentage recoveries of binary mixture of TELM were found to be 97.81±1.05 (263.2nm) and 96.60±1.91 (278.4nm) and for HCTZ were found to be 97.47±1.41 (287.2), 98.16± 1.45 (304.8nm) by proposed method. This method was successfully applied for the analysis of commercial tablet formulation, with no interference from excipients as indicated by the recovery study results. The proposed method was validated, statistically analyzed, compared with reported HPLC method and can be successfully applied for the routine analysis.

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Stability-Indicating RP-HPLC Method for Simultaneous Quantification of Ombitasvir, Paritaprevir and Ritonavir in Tablet Dosage Form

Asian Journal of Chemistry ◽

10.14233/ajchem.2018.21215 ◽

2018 ◽

Vol 30 (6) ◽

pp. 1277-1283 ◽

Cited By ~ 1

Author(s):

Mangamma Kuna ◽

Gowri Sankar Dannana

Keyword(s):

Dosage Form ◽

Hplc Method ◽

Stability Indicating ◽

Simultaneous Quantification ◽

Rp Hplc ◽

Tablet Dosage

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DETERMINATION AND VALIDATION OF METOPROLOL AND ATORVASTATIN SIMULTANEOUSLY BY RP-HPLC METHOD IN TABLET DOSAGE FORM

Indian Research Journal of Pharmacy and Science ◽

10.21276/irjps.2019.6.3.6 ◽

2019 ◽

Vol 6 (3) ◽

pp. 1952-1965

Author(s):

Farheen Begum ◽

◽

R. Vani ◽

Keyword(s):

Dosage Form ◽

Hplc Method ◽

Rp Hplc ◽

Tablet Dosage

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Development and Validation of RP-HPLC Method for the Estimation of Sitagliptin Phosphate in Tablet Dosage Form

International Journal of Applied Pharmaceutical Sciences and Research ◽

10.21477/ijapsr.v2i3.8099 ◽

2017 ◽

Vol 2 (03) ◽

pp. 41-45

Author(s):

Sireesha D ◽

Sai Lakshmi E ◽

Sravya E ◽

Vasudha Bakshi

Keyword(s):

High Performance ◽

Dosage Form ◽

Room Temperature ◽

Hplc Method ◽

Pharmaceutical Dosage Form ◽

Rp Hplc ◽

Ich Guidelines ◽

Development And Validation ◽

Tablet Dosage ◽

Isocratic Mode

A new simple, rapid, specific, accurate, precise and novel Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the estimation of Sitagliptin Phosphate in the pharmaceutical dosage form. The chromatographic separation for Sitagliptin was achieved with mobile phase containing methanol, Thermoscientific C18 column, (250x4.6 particle size of 5μ) at room temperature and UV detection at 248 nm. The compounds were eluted in the isocratic mode at a flow rate of 1ml/min. The retention time of Sitagliptin was 1.91min. The above method was validated in terms of linearity, accuracy, precision, LOD and LOQ in accordance with ICH guidelines.

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