DNA Breaks Due to Replication Stress Limit the Developmental Potential of Human Preimplantation Embryos

2021 ◽  
Author(s):  
Katherine L. Palmerola ◽  
Selma Amrane ◽  
Alejandro De Los Angeles ◽  
Shuangyi Xu ◽  
Michael V. Zuccaro ◽  
...  
Keyword(s):  
Replication Stress ◽  
Preimplantation Embryos ◽  
Dna Breaks ◽  
Developmental Potential ◽  
Stress Limit ◽  
Human Preimplantation Embryos

scholarly journals Replication stress limits developmental potential of human preimplantation embryos

2019 ◽  
Vol 112 (3) ◽  
pp. e128
Author(s):  
Katherine L. Palmerola ◽  
Eric J. Forman ◽  
Rogerio A. Lobo ◽  
Dieter M. Egli
Keyword(s):  
Replication Stress ◽  
Preimplantation Embryos ◽  
Developmental Potential ◽  
Human Preimplantation Embryos

scholarly journals Dynamics and clinical relevance of maternal mRNA clearance during the oocyte-to-embryo transition in humans

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Qian-Qian Sha ◽  
Wei Zheng ◽  
Yun-Wen Wu ◽  
Sen Li ◽  
Lei Guo ◽  
...  
Keyword(s):  
Mrna Decay ◽  
Meiotic Spindle ◽  
Preimplantation Embryos ◽  
Human Embryos ◽  
Developmental Potential ◽  
Maternal Mrna ◽  
Before And After ◽  
Maternal Transcripts ◽  
Human Preimplantation Embryos

Abstract Maternal mRNA clearance is an essential process that occurs during maternal-to-zygotic transition (MZT). However, the dynamics, functional importance, and pathological relevance of maternal mRNA decay in human preimplantation embryos have not yet been analyzed. Here we report the zygotic genome activation (ZGA)-dependent and -independent maternal mRNA clearance processes during human MZT and demonstrate that subgroups of human maternal transcripts are sequentially removed by maternal (M)- and zygotic (Z)-decay pathways before and after ZGA. Key factors regulating M-decay and Z-decay pathways in mouse have similar expression pattern during human MZT, suggesting that YAP1-TEAD4 transcription activators, TUT4/7-mediated mRNA 3ʹ-oligouridylation, and BTG4/CCR4-NOT-induced mRNA deadenylation may also be involved in the regulation of human maternal mRNA stability. Decreased expression of these factors and abnormal accumulation of maternal transcripts are observed in the development-arrested embryos of patients who seek assisted reproduction. Defects of M-decay and Z-decay are detected with high incidence in embryos that are arrested at the zygote and 8-cell stages, respectively. In addition, M-decay is not found to be affected by maternal TUBB8 mutations, although these mutations cause meiotic cell division defects and zygotic arrest, which indicates that mRNA decay is regulated independent of meiotic spindle assembly. Considering the correlations between maternal mRNA decay defects and early developmental arrest of in vitro fertilized human embryos, M-decay and Z-decay pathway activities may contribute to the developmental potential of human preimplantation embryos.

scholarly journals Mosaic human preimplantation embryos and their developmental potential in a prospective, non-selection clinical trial

2021 ◽  
Author(s):  
Antonio Capalbo ◽  
Maurizio Poli ◽  
Laura Rienzi ◽  
Laura Girardi ◽  
Cristina Patassini ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Preimplantation Embryos ◽  
Developmental Potential ◽  
Human Preimplantation Embryos

scholarly journals Advanced maternal age perturbs mouse embryo development and alters the phenotype of derived embryonic stem cells

2021 ◽  
pp. 1-11
Author(s):  
Pooja Khurana ◽  
Neil R. Smyth ◽  
Bhavwanti Sheth ◽  
Miguel A. Velazquez ◽  
Judith J. Eckert ◽  
...  
Keyword(s):  
Maternal Age ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Advanced Maternal Age ◽  
Preimplantation Embryos ◽  
Dependent Manner ◽  
Ki 67 ◽  
Altered Expression ◽  
Developmental Potential ◽  
Developmental Mechanisms

Abstract Advanced maternal age (AMA) is known to reduce fertility, increases aneuploidy in oocytes and early embryos and leads to adverse developmental consequences which may associate with offspring lifetime health risks. However, investigating underlying effects of AMA on embryo developmental potential is confounded by the inherent senescence present in maternal body systems further affecting reproductive success. Here, we describe a new model for the analysis of early developmental mechanisms underlying AMA by the derivation and characterisation of mouse embryonic stem cell (mESC-like) lines from naturally conceived embryos. Young (7–8 weeks) and Old (7–8 months) C57BL/6 female mice were mated with young males. Preimplantation embryos from Old dams displayed developmental retardation in blastocyst morphogenesis. mESC lines established from these blastocysts using conventional techniques revealed differences in genetic, cellular and molecular criteria conserved over several passages in the standardised medium. mESCs from embryos from AMA dams displayed increased incidence of aneuploidy following Giemsa karyotyping compared with those from Young dams. Moreover, AMA caused an altered pattern of expression of pluripotency markers (Sox2, OCT4) in mESCs. AMA further diminished mESC survival and proliferation and reduced the expression of cell proliferation marker, Ki-67. These changes coincided with altered expression of the epigenetic marker, Dnmt3a and other developmental regulators in a sex-dependent manner. Collectively, our data demonstrate the feasibility to utilise mESCs to reveal developmental mechanisms underlying AMA in the absence of maternal senescence and with reduced animal use.

scholarly journals Transmission ratio distortion in the myotonic dystrophy locus in human preimplantation embryos

2006 ◽  
Vol 14 (3) ◽  
pp. 299-306 ◽  
Author(s):  
Nicola L Dean ◽  
J Concepción Loredo-Osti ◽  
T Mary Fujiwara ◽  
Kenneth Morgan ◽  
Seang Lin Tan ◽  
...  
Keyword(s):  
Myotonic Dystrophy ◽  
Transmission Ratio Distortion ◽  
Preimplantation Embryos ◽  
Transmission Ratio ◽  
Ratio Distortion ◽  
Human Preimplantation Embryos

Improvement of the culture conditions for the development of human preimplantation embryos

1990 ◽  
Vol 5 (2) ◽  
pp. 217-220 ◽  
Author(s):  
A.L. Muggleton-Harris ◽  
I. Findlay ◽  
D.G. Whittingham
Keyword(s):  
Culture Conditions ◽  
Preimplantation Embryos ◽  
Human Preimplantation Embryos

scholarly journals P59 Over-expression of ATR and XRCC1 as potential biomarkers for poor prognosis in human preimplantation embryos

2012 ◽  
Vol 24 ◽  
pp. S71
Author(s):  
M. Bazrgar ◽  
H. Gourabi ◽  
P. Eftekhari-Yazdi ◽  
H. Vaziri ◽  
M. Fakhri ◽  
...  
Keyword(s):  
Poor Prognosis ◽  
Preimplantation Embryos ◽  
Over Expression ◽  
Human Preimplantation Embryos ◽  
Potential Biomarkers

Detection of chromosomal abnormalities in human preimplantation embryos using FISH

1996 ◽  
Vol 13 (2) ◽  
pp. 137-139 ◽  
Author(s):  
Joyce C. Harper ◽  
Joy D. A. Delhanty
Keyword(s):  
Chromosomal Abnormalities ◽  
Preimplantation Embryos ◽  
Human Preimplantation Embryos

scholarly journals Biological and Clinical Significance of Mosaicism in Human Preimplantation Embryos

2021 ◽  
Vol 9 (2) ◽  
pp. 18
Author(s):  
Ioanna Bouba ◽  
Elissavet Hatzi ◽  
Paris Ladias ◽  
Prodromos Sakaloglou ◽  
Charilaos Kostoulas ◽  
...  
Keyword(s):  
Assisted Reproduction ◽  
Basic Research ◽  
Blastocyst Stage ◽  
Preimplantation Embryos ◽  
New Approach ◽  
Assisted Reproduction Technology ◽  
Clinical Utilization ◽  
Art Practices ◽  
In Utero Development ◽  
Human Preimplantation Embryos

Applications and indications of assisted reproduction technology are expanding, but every new approach is under scrutiny and thorough consideration. Recently, groups of assisted reproduction experts have presented data that support the clinical use of mosaic preimplantation embryos at the blastocyst stage, previously excluded from transfer. In the light of published contemporary studies, with or without clinical outcomes, there is growing evidence that mosaic embryos have the capacity for further in utero development and live birth. Our in-depth discussion will enable readers to better comprehend current developments. This expansion into the spectrum of ART practices requires further evidence and further theoretical documentation, basic research, and ethical support. Therefore, if strict criteria for selecting competent mosaic preimplantation embryos for further transfer, implantation, fetal growth, and healthy birth are applied, fewer embryos will be excluded, and more live births will be achieved. Our review aims to discuss the recent literature on the transfer of mosaic preimplantation embryos. It also highlights controversies as far as the clinical utilization of preimplantation embryos concerns. Finally, it provides the appropriate background to elucidate and highlight cellular and genetic aspects of this novel direction.

scholarly journals Quantitative and qualitative changes of mitochondria in human preimplantation embryos

2017 ◽  
Vol 34 (5) ◽  
pp. 573-580 ◽  
Author(s):  
Shu Hashimoto ◽  
Naoharu Morimoto ◽  
Masaya Yamanaka ◽  
Hiroshi Matsumoto ◽  
Takayuki Yamochi ◽  
...  
Keyword(s):  
Preimplantation Embryos ◽  
Human Preimplantation Embryos ◽  
Qualitative Changes
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