scholarly journals 2P097 Direct observation of the RuvA-RuvB mediated branch migration of Holliday junction DNA with single-molecule analysis

2004 ◽  
Vol 44 (supplement) ◽  
pp. S134
Author(s):  
Y.-W. Han ◽  
T. Tani ◽  
M. Hayashi ◽  
T. Hishida ◽  
H. Iwasaki ◽  
...  
Keyword(s):  
Direct Observation ◽  
Single Molecule ◽  
Holliday Junction ◽  
Branch Migration ◽  
Single Molecule Analysis

scholarly journals Holliday junction dynamics and branch migration: Single-molecule analysis

2005 ◽  
Vol 102 (23) ◽  
pp. 8186-8191 ◽  
Author(s):  
M. Karymov ◽  
D. Daniel ◽  
O. F. Sankey ◽  
Y. L. Lyubchenko
Keyword(s):  
Single Molecule ◽  
Holliday Junction ◽  
Branch Migration ◽  
Single Molecule Analysis

scholarly journals Structure, Dynamics, and Branch Migration of a DNA Holliday Junction: A Single-Molecule Fluorescence and Modeling Study

2008 ◽  
Vol 95 (9) ◽  
pp. 4372-4383 ◽  
Author(s):  
Mikhail A. Karymov ◽  
Mathivanan Chinnaraj ◽  
Aleksey Bogdanov ◽  
Annankoil R. Srinivasan ◽  
Guohui Zheng ◽  
...  
Keyword(s):  
Single Molecule ◽  
Holliday Junction ◽  
Branch Migration ◽  
Single Molecule Fluorescence ◽  
Structure Dynamics ◽  
Modeling Study

scholarly journals A bacterial resolvase first exploits, then constrains intrinsic dynamics of the Holliday junction to direct recombination

2019 ◽  
Author(s):  
Sujay Ray ◽  
Nibedita Pal ◽  
Nils G. Walter
Keyword(s):  
Cluster Analysis ◽  
Homologous Recombination ◽  
Single Molecule ◽  
Holliday Junction ◽  
Branch Migration ◽  
Migration Dynamics ◽  
And Cluster Analysis ◽  
Genome Repair

AbstractHomologous recombination forms and resolves an entangled DNA Holliday Junction (HJ) critical for achieving genome repair. We use single-molecule observation and cluster analysis to probe how prototypic bacterial resolvase RuvC selects two of four possible HJ strands for cleavage. RuvC first exploits, then constrains intrinsic HJ isomer exchange and branch migration dynamics to direct cleavage toward only a desired, catalytically competent HJ conformation, thus controlling recombination products.

scholarly journals Single Molecule Analysis of differential functional mechanisms of MtRecG while being exposed to variants of stalled replication-fork

2021 ◽  
Author(s):  
Debolina Bandyopadhyay ◽  
Padmaja P Mishra
Keyword(s):  
Single Molecule ◽  
Vital Role ◽  
Holliday Junction ◽  
Asymmetric Mode ◽  
Molecular Surveillance ◽  
Efficient Gene ◽  
Leading Strand ◽  
Single Molecule Analysis ◽  
Functional Mechanisms ◽  
Stalled Fork

AbstractHelicases are motor proteins involved in multiple activities to carry out manipulation of the nucleic acids for efficient gene regulation. In case of roadblocks that can lead the replication machinery to get halted, a complex molecular surveillance system utilizing helicases as its key player ensures the halted fork to resume its duplication process. RecG, belonging to the category of Superfamily-2 plays a vital role in rescuing different kinds of stalled fork. Here, through adoption of single-molecule techniques we have attempted to probe the DNA unwinding features by RecG and tried to capture several stages of genetic rearrangement. An elevated processivity of RecG has been observed for the kinds of stalled fork where progression of lagging daughter strand is ahead than that of the leading strand. Through precise alteration of its function in terms of unwinding, depending upon the substrate DNA, RecG catalyzes the formation of Holliday junction from a stalled fork DNA. In summary, we have featured that RecG adopts asymmetric mode of locomotion to unwind the lagging daughter strand to facilitate Holliday junction creation which acts as a suitable intermediate for recombinational repair pathway.

Sign in / Sign up

Export Citation Format

Share Document