scholarly journals Protocol for high-sensitivity/long linear-range spectrofluorimetric DNA quantification using ethidium bromide

BioTechniques ◽  
2007 ◽  
Vol 43 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Veronica Bonasera ◽  
Saverio Alberti ◽  
Andrea Sacchetti
2021 ◽  
Vol 11 (11) ◽  
pp. 1774-1780
Author(s):  
Shanji Fan ◽  
Hong Huang ◽  
Hong Chen ◽  
Jiachi Xu ◽  
Zecheng Hu ◽  
...  

A CdS nanocrystal enhanced TiO2 nanotubes (CdS@TiO2 NATs) photoelectrode was prepared via successive ionic layer adsorption and reaction (SILAR) of CdS on the surface of TiO2 NATs. A HS-aptamer owing a specific binding toward cytochrome c was modified onto the CdS@TiO2 NATs, which resulting a decrease in the photoelectrical current intensity. Cytochrome c is therefore quantified based on the decrease in photoelectrical current. High specificity and high sensitivity were obtained with a linear range from 3 pM to 80 nM, and a limit of detection of 2.53 pM.


2011 ◽  
Vol 11 (1) ◽  
pp. 97 ◽  
Author(s):  
Tom Boonefaes ◽  
Erica Houthuys ◽  
Rafael Van den Bergh ◽  
Seppe Vander Beken ◽  
Geert Raes ◽  
...  

RSC Advances ◽  
2015 ◽  
Vol 5 (124) ◽  
pp. 102877-102884 ◽  
Author(s):  
He Mei ◽  
Huimin Wu ◽  
Wenqin Wu ◽  
Shengfu Wang ◽  
Qinghua Xia

A electrochemical sensor based on PtNi/MWCNTs can detect hydrogen peroxide and glucose with wide linear range and high sensitivity.


Micromachines ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1485
Author(s):  
Fei Ge ◽  
Liye Zhao ◽  
Yang Zhang

Surface acoustic wave gyroscopes (SAWGs), as a kind of all-solid-state micro-electro-mechanical system (MEMS) gyroscopes, can work normally under extremely high-impact environmental conditions. Among the current SAWGs, amplitude-modulated gyroscopes (AMGs) are all based on the same gyro effect, which was proved weak, and their sensitivity and intensity of the output are both lower than frequency-modulated gyroscopes (FMGs). However, because FMGs need to process a series of frequency signals, their signal processing and circuits are far less straightforward and simple than AMGs. In order to own both high-sensitivity and simple signal processing, a novel surface acoustic traveling wave gyroscope based on amplitude modulation is proposed, using one-dimensional phononic crystals (PCs) in this paper. In view of its specific structure, the proposed gyroscope consists of a surface acoustic wave oscillator and a surface acoustic wave delay line within a one-dimensional phononic crystal with a high-Q defect mode. In this paper, the working principle is analyzed theoretically through the partial wave method (PWM), and the gyroscopes with different numbers of PCs are also designed and studied by using the finite element method (FEM) and multiphysics simulation. The research results demonstrate that under a 1 V oscillator voltage output, the higher sensitivity of −23.1 mV·(rad/s)−1 in the linear range from −8 rad/s to 8 rad/s is reached when the gyro with three PC walls, and the wider linear range from −15 rad/s to 17.5 rad/s with the sensitivity of −6.7 mV·(rad/s)−1 with only one PC wall. Compared with the existing AMGs using metal dots to enhance the gyro effect, the sensitivity of the proposed gyro is increased by 15 to 112 times, and the linear range is increased by 4.6 to 186 times, even without the enhancement of the metal dots.


Small ◽  
2018 ◽  
Vol 14 (31) ◽  
pp. 1801520 ◽  
Author(s):  
Yan Huang ◽  
Yun Chen ◽  
Xiangyu Fan ◽  
Ningqi Luo ◽  
Shuang Zhou ◽  
...  

2019 ◽  
Vol 123 ◽  
pp. 244-250 ◽  
Author(s):  
Pawan Jolly ◽  
Joshua Rainbow ◽  
Anna Regoutz ◽  
Pedro Estrela ◽  
Despina Moschou

2021 ◽  
Author(s):  
Maho Saita ◽  
Kyoko Aikawa ◽  
Kenji Ohgane

This protocol offers an safer alternative to the ethidium bromide-based DNA quantification protocol, utilizing an DNA-selective dye EzFluoroStain DNA (WSE-7130, ATTO corporation, Tokyo, Japan). This protocol allows to quantify about 2-1000 ng DNA/well, using a standard fluorescent plate reader.


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