scholarly journals Delicaflavone Reverses Cisplatin Resistance via Endoplasmic Reticulum Stress Signaling Pathway in Non-Small Cell Lung Cancer Cells

2020 ◽  
Vol Volume 13 ◽  
pp. 10315-10322
Author(s):  
Xuewen Wang ◽  
Bing Chen ◽  
Danfen Xu ◽  
Zhijun Li ◽  
Yuxia Sui ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Small Cell Lung Cancer ◽  
Cancer Cells ◽  
Cisplatin Resistance ◽  
Small Cell ◽  
Stress Signaling ◽  
Small Cell Lung ◽  
Stress Signaling Pathway

Oxidative and endoplasmic reticulum stress signaling are involved in dehydrocostuslactone-mediated apoptosis in human non-small cell lung cancer cells

Lung Cancer ◽  
2010 ◽  
Vol 68 (3) ◽  
pp. 355-365 ◽  
Author(s):  
Jen-Yu Hung ◽  
Ya-Ling Hsu ◽  
Wen-Chiu Ni ◽  
Ying-Ming Tsai ◽  
Chih-Jen Yang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Small Cell Lung Cancer ◽  
Cancer Cells ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Lung Cancer Cells ◽  
Stress Signaling ◽  
Small Cell Lung

A novel derivative of tetrandrine (H1) induces endoplasmic reticulum stress-mediated apoptosis and prosurvival autophagy in human non-small cell lung cancer cells

Tumor Biology ◽  
2016 ◽  
Vol 37 (8) ◽  
pp. 10403-10413 ◽  
Author(s):  
Yidan Lin ◽  
Yu Wang ◽  
Xianfang Liu ◽  
Jiamei Yan ◽  
Ling Su ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Small Cell Lung Cancer ◽  
Cancer Cells ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Lung Cancer Cells ◽  
Small Cell Lung

scholarly journals Altered Ca2+-Homeostasis of Cisplatin-Treated and Low Level Resistant Non-Small-Cell and Small-Cell Lung Cancer Cells

2009 ◽  
Vol 31 (4) ◽  
pp. 301-315
Author(s):  
Kathrin Schrödl ◽  
Hamza Oelmez ◽  
Martin Edelmann ◽  
Rudolf Maria Huber ◽  
Albrecht Bergner
Keyword(s):  
Lung Cancer ◽  
Endoplasmic Reticulum ◽  
Small Cell Lung Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Cisplatin Resistance ◽  
Small Cell ◽  
Small Cell Lung ◽  
Low Level ◽  
Level Resistance

Background: Chemotherapy often leads to encouraging responses in lung cancer. But, in the course of the treatment, resistance to chemotherapy ultimately limits the life expectancy of the patient. We aimed at investigating if treatment with cisplatin alters the intracellular Ca2+-homeostasis of lung cancer cells and how this may be related to cisplatin resistance.Methods: The squamous cell lung carcinoma cell line EPLC M1 and the small cell lung cancer cell line H1339 were exposed to cisplatin analogue to the in vivo pharmacokinetics. Changes in the cytoplasmic Ca2+-concentration ([Ca2+]c) were recorded using fluorescence microscopy. Protein expression was quantified using immuno-fluorescence and Western Blot analysis. Changes in gene expression were accomplished by small-interfering (si) RNA techniques.Results: Four “cycles” of cisplatin led to low level resistance in EPLC and H1339 cells. In the low level resistant cell clones, the Ca2+-content of the endoplasmic reticulum (ER) was decreased. In low level resistant EPLC cells, this was correlated with an increased expression of the inositol-1,4,5-trisphosphate receptor (IP3R). Inhibiting the increased expression of IP3R using siRNA, the low level resistance could be reversed. In low level resistant H1339 cells, the decreased Ca2+-content of the ER was correlated with a decreased expression of sarco/endoplasmic reticulum Ca2+-ATPases (SERCA). Decreasing the expression of SERCA in naïve H1339 cells resulted in low level cisplatin resistance.Conclusion: We conclude that cisplatin therapy leads to a decreased Ca2+-content of the ER thereby inducing low level resistance. This is caused by upregulation of the IP3R in EPLC and decreased expression of SERCA in H1339 cells.

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