Lung Cancer Cells
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2021 ◽  
Vol 22 (6) ◽  
Yue Qian ◽  
Hongliang Dai ◽  
Hongyu Li

2021 ◽  
Parimal Karmakar ◽  
Sougata Ghosh Chowdhury ◽  
Rachayeeta Ray ◽  
Debalina Bhattacharya

Abstract Exosomes are one type of small extracellular vesicles having a size range of 30–150 nm and secreted by the endosomal compartment of most eukaryotic cells. It has been found that exosomes can serve as a communicating vehicle to transfer information among cells and thus can be associated with numerous physiological and pathological functions. In this study, we have isolated exosomes from two different human cancer cell lines. Isolated exosomes were characterized by scanning electron microscopy, DLS and by western blotting. It was observed that exosomes isolated from mock treated human lung epithelial carcinoma (A549) cells or Hela cells exerted growth arrest to the human prostate carcinoma (PC3) cells, but no growth arrest was observed in case of normal human lung fibroblast cell line (WI38). Additionally, exosomes isolated from PC3 cells have no effect on PC3 cells. This is also true for exosomes isolated from H2O2 induced senescent human lung cancer cells (A549). Analysis of exosome content by western blotting reveals the presence of PTEN in the exosome of lung cancer cells. Functional analysis of PTEN pathways in PC3 cells indicates the inactivation of Akt in exosome treated cells. Therefore, from our study we have concluded that exosomes secreted from A549 cells which contain functional PTEN, may be used for delivery of PTEN to cancer cells without functional PTEN.

Lin Lin ◽  
Yongxia Bao ◽  
Miao Tian ◽  
Qiu Ren ◽  
Wei Zhang

2021 ◽  
pp. 2101999
Fan Chen ◽  
Jinpeng Liu ◽  
Robert M. Flight ◽  
Kassandra J. Naughton ◽  
Alexsandr Lukyanchuk ◽  

Xiaowen Hu ◽  
Kandasamy Saravanakumar ◽  
Anbazhagan Sathiyaseelan ◽  
Vinothkumar Rajamanickam ◽  
Myeong-Hyeon Wang

CHEST Journal ◽  
2021 ◽  
Vol 160 (4) ◽  
pp. A1638
Seihoon Yang ◽  
Misung Kim ◽  
Sohui Kim ◽  
Minsuk Kim

Optik ◽  
2021 ◽  
pp. 168157
Xianglin Fang ◽  
Shaoxin Li ◽  
Qiuyue Fu ◽  
Peng Wang ◽  
Xingda Wu ◽  

2021 ◽  
Vol 50 (9) ◽  
pp. 2663-2674
Iwan Dini ◽  
Nunuk Hariani Soekamto ◽  
Firdaus Firdaus ◽  
Unang Supratman ◽  
Jalifah Latip

Alkaloid caulerpin (1), along with β-sitosterol (2), were isolated from the n-hexane extract of the macroalga Halimeda cylindracea Decaisne. The chemical structure was identified by a spectroscopic method including IR, MS, UV, NMR 1D, NMR 2D, and comparison with data of spectra previously reported. Compounds (1) and (2) were isolated for the first time from this macroalga. Compund (1) were evaluated for their cytotoxicity activity against NCL-H460 lung cancer cells in vitro and showed moderate activity with IC50 value of 20.05 µg/mL.

2021 ◽  
Vol 11 (19) ◽  
pp. 8866
Myong Jin Lee ◽  
Geum Jin Kim ◽  
Myoung-Sook Shin ◽  
Jimin Moon ◽  
Sungjin Kim ◽  

Chemical investigations of Aquimarina sp. MC085, which suppressed TGF-β-induced epithelial–mesenchymal transition (EMT) in A549 human lung cancer cells, led to the isolation of compounds 1–3. Structural characterization using spectroscopic data analyses in combination with Marfey’s analysis revealed that they were two diketopiperazines [cyclo(l-Pro-l-Leu) (1) and cyclo(l-Pro-l-Ile) (2)] and one N-phenethylacetamide (3). Cyclo(l-Pro-l-Leu) (1) and N-phenethylactamide (3) inhibited the TGF-β/Smad pathway and suppressed the metastasis of A549 cells by affecting TGF-β-induced EMT. However, cyclo(l-Pro-l-Ile) (2) downregulated mesenchymal factors via a non-Smad-mediated signaling pathway.

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