scholarly journals Digital PCR Detection of mtDNA/gDNA Ratio in Embryo Culture Medium for Prediction of Embryo Development Potential

2021 ◽  
Vol Volume 14 ◽  
pp. 521-531
Author(s):  
Qing Zhang ◽  
Hong Ji ◽  
Jian Shi ◽  
Longmei Wang ◽  
Lu Ding ◽  
...  
Keyword(s):  
Embryo Development ◽  
Embryo Culture ◽  
Culture Medium ◽  
Digital Pcr ◽  
Pcr Detection ◽  
Development Potential ◽  
Embryo Culture Medium

scholarly journals Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

animal ◽  
2013 ◽  
Vol 7 (3) ◽  
pp. 455-462 ◽  
Author(s):  
C.J. Ahumada ◽  
I. Salvador ◽  
A. Cebrian-Serrano ◽  
R. Lopera ◽  
M.A. Silvestre
Keyword(s):  
Growth Factors ◽  
Embryo Development ◽  
Embryo Culture ◽  
Culture Medium ◽  
Bovine Embryos ◽  
Embryo Culture Medium

scholarly journals PS48 can replace bovine serum albumin in pig embryo culture medium, and improve in vitro embryo development by phosphorylating AKT

2015 ◽  
Vol 82 (4) ◽  
pp. 315-320 ◽  
Author(s):  
Lee D. Spate ◽  
Alana Brown ◽  
Bethany K. Redel ◽  
Kristin M. Whitworth ◽  
Randall S. Prather
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Embryo Development ◽  
Embryo Culture ◽  
Bovine Serum ◽  
Culture Medium ◽  
Embryo Culture Medium

Supplementation with cysteamine during maturation and embryo culture on embryo development of prepubertal goat oocytes selected by the brilliant cresyl blue test

Zygote ◽  
2003 ◽  
Vol 11 (4) ◽  
pp. 347-354 ◽  
Author(s):  
Aixa Urdaneta ◽  
Ana-Raquel Jiménez-Macedo ◽  
Dolors Izquierdo ◽  
Maria-Teresa Paramio
Keyword(s):  
Embryo Development ◽  
Embryo Culture ◽  
Culture Medium ◽  
Blastocyst Development ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Development Rates ◽  
Embryo Culture Medium ◽  
Normal Fertilization

Our previous studies have shown that the addition of 100 μM cysteamine to the in vitro maturation (IVM) medium increased the embryo development of prepubertal goat oocytes. The aim of the present study was to evaluate the effect of adding different concentrations of cysteamine to the IVM medium and to the in vitro embryo culture medium (IVC) on the embryo development of prepubertal goat oocytes selected by the brilliant cresyl blue (BCB) test. Oocytes were exposed to BCB and classified as: oocytes with a blue cytoplasm or grown oocytes (BCB+) or oocytes without blue cytoplasm or growing oocytes (BCB−). In Experiment 1, oocytes were matured in a conventional IVM medium supplemented with 100 μM, 200 μM or 400 μM cysteamine. In Experiment 2, oocytes were matured with 400 μM cysteamine and following in vitro fertilization (IVF) were cultured in SOF medium supplemented with 50 μM and 100 μM cysteamine. In Experiment 1, BCB+ oocytes matured with 100 μM and 200 μM cysteamine showed higher normal fertilization and embryo development rates than BCB− oocytes. Oocytes matured with 400 μM cysteamine did not present these differences between BCB+ and BCB− oocytes. In Experiment 2, the addition of 50 μM and 100 μM cysteamine to culture medium did not affect the proportion of total embryos obtained from BCB+ oocytes (35.89% and 38.29%, respectively) but was significantly different in BCB− oocytes (34.23% and 29.04%, respectively, P<0.05). In conclusion, the addition of 400 μM cysteamine to the IVM improved normal fertilization and embryo development of BCB− oocytes at the same rates as those obtained from BCB+ oocytes. The proportions of morulae plus blastocyst development were not affected by the treatments.

scholarly journals Supplement of Betaine into Embryo Culture Medium Can Rescue Injury Effect of Ethanol on Mouse Embryo Development

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Di Zhang ◽  
Huaijiang Jing ◽  
Changfeng Dou ◽  
Ling Zhang ◽  
Xiaoqing Wu ◽  
...  
Keyword(s):  
Embryo Development ◽  
Mouse Embryo ◽  
Embryo Culture ◽  
Culture Medium ◽  
Mouse Embryo Development ◽  
Embryo Culture Medium

scholarly journals Non-invasive Metabolomic Profiling of Embryo Culture Medium Using Raman Spectroscopy With Deep Learning Model Predicts the Blastocyst Development Potential of Embryos

2021 ◽  
Vol 12 ◽  
Author(s):  
Wei Zheng ◽  
Shuoping Zhang ◽  
Yifan Gu ◽  
Fei Gong ◽  
Lingyin Kong ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Deep Learning ◽  
Embryo Culture ◽  
Culture Medium ◽  
Classification Model ◽  
Cleavage Stage ◽  
Blastocyst Development ◽  
Development Potential ◽  
Non Invasive ◽  
Embryo Culture Medium

Purpose: This study aimed to establish a non-invasive predicting model via Raman spectroscopy for evaluating the blastocyst development potential of day 3 high-quality cleavage stage embryos.Methods: Raman spectroscopy was used to detect the metabolic spectrum of spent day 3 (D3) embryo culture medium, and a classification model based on deep learning was established to differentiate between embryos that could develop into blastocysts (blastula) and that could not (non-blastula). The full-spectrum data for 80 blastula and 48 non-blastula samples with known blastocyst development potential from 34 patients were collected for this study.Results: The accuracy of the predicting method was 73.53% and the main different Raman shifts between blastula and non-blastula groups were 863.5, 959.5, 1,008, 1,104, 1,200, 1,360, 1,408, and 1,632 cm–1 from 80 blastula and 48 non-blastula samples by the linear discriminant method.Conclusion: This study demonstrated that the developing potential of D3 cleavage stage embryos to the blastocyst stage could be predicted with spent D3 embryo culture medium using Raman spectroscopy with deep learning classification models, and the overall accuracy reached at 73.53%. In the Raman spectroscopy, ribose vibration specific to RNA were found, indicating that the difference between the blastula and non-blastula samples could be due to materials that have similar structure with RNA. This result could be used as a guide for biomarker development of embryo quality assessment in the future.

Infection in embryo culture medium

2021 ◽  
pp. 509-514
Author(s):  
Alison Campbell ◽  
Louise Best
Keyword(s):  
Embryo Culture ◽  
Culture Medium ◽  
Embryo Culture Medium

Effects of changing culture medium on preimplantation embryo development in rabbit

Zygote ◽  
2021 ◽  
pp. 1-6
Author(s):  
Haixia Wang ◽  
Wenbin Cao ◽  
Huizhong Hu ◽  
Chenglong Zhou ◽  
Ziyi Wang ◽  
...  
Keyword(s):  
Embryo Development ◽  
Embryo Culture ◽  
Culture Medium ◽  
Cell Number ◽  
Apoptotic Index ◽  
Total Cell ◽  
Toxic Substances ◽  
Total Cell Number ◽  
Preimplantation Embryo Development

Summary Many studies have focused on the optimization of the composition of embryo culture medium; however, there are few studies involving the effect of a culture medium changing procedure on the preimplantation development of embryos. In this study, three groups were designed: a non-renewal group, a renewal group and a half-renewal group. The levels of reactive oxygen species (ROS), apoptotic index, blastocyst ratio and blastocyst total cell number were analyzed in each group. The results showed that the ROS level and the apoptotic index of blastocyst in the non-renewal group were significantly higher than in the renewal group and the half-renewal group (P < 0.05). The blastocyst ratio and blastocyst total cell number were significantly higher in the half-renewal group than that in non-renewal group and the renewal group (P < 0.05). These results demonstrated that the procedure of changing the culture medium influenced ROS level, apoptotic index, blastocyst ratio and total cell number of blastocysts. In addition, the result suggested that changing the culture medium may lead to a loss of important regulatory factors for embryos, while not changing the culture medium may lead to the accumulation of toxic substances. Half-renewal can alleviate the defects of both no renewal and renewal, and benefit embryo development. This study will be of high value as a reference for the optimization of embryo culture in vitro, and is very significant for assisted reproduction.

Sign in / Sign up

Export Citation Format

Share Document