scholarly journals Extraction and characterization of Antigenic S- lipopolysaccharides Brucella abortus S99.

2021 ◽  
Vol 41 (1) ◽  
pp. 154-158
Author(s):  
Cleopatra Roushdy ◽  
Mohamed Gouda ◽  
Abdel-Moneim Moustafa ◽  
Faysal Ibrahim ◽  
Essam El-bauomy ◽  
...  
Keyword(s):  
Brucella Abortus

scholarly journals Characterization of the Urease Operon of Brucella abortus and Assessment of Its Role in Virulence of the Bacterium

2006 ◽  
Vol 75 (2) ◽  
pp. 774-780 ◽  
Author(s):  
Félix J. Sangari ◽  
Asunción Seoane ◽  
María Cruz Rodríguez ◽  
Jesús Agüero ◽  
Juan M. García Lobo
Keyword(s):  
Brucella Abortus ◽  
Urease Activity ◽  
Strong Acid ◽  
Oral Route ◽  
Open Reading Frames ◽  
Human Brucellosis ◽  
Major Route

ABSTRACT Most members of the genus Brucella show strong urease activity. However, the role of this enzyme in the pathogenesis of Brucella infections is poorly understood. We isolated several Tn5 insertion mutants deficient in urease activity from Brucella abortus strain 2308. The mutations of most of these mutants mapped to a 5.7-kbp DNA region essential for urease activity. Sequencing of this region, designated ure1, revealed the presence of seven open reading frames corresponding to the urease structural proteins (UreA, UreB, and UreC) and the accessory proteins (UreD, UreE, UreF, and UreG). In addition to the urease genes, another gene (cobT) was identified, and inactivation of this gene affected urease activity in Brucella. Subsequent analysis of the previously described sequences of the genomes of Brucella spp. revealed the presence of a second urease cluster, ure2, in all them. The ure2 locus was apparently inactive in B. abortus 2308. Urease-deficient mutants were used to evaluate the role of urease in Brucella pathogenesis. The urease-producing strains were found to be resistant in vitro to strong acid conditions in the presence of urea, while urease-negative mutants were susceptible to acid treatment. Similarly, the urease-negative mutants were killed more efficiently than the urease-producing strains during transit through the stomach. These results suggested that urease protects brucellae during their passage through the stomach when the bacteria are acquired by the oral route, which is the major route of infection in human brucellosis.

Development and characterization of a modified Komarov's bullet for ballistic delivery of live Brucella abortus strains 82 and 19 to cattle and bison

Vaccine ◽  
2010 ◽  
Vol 28 ◽  
pp. F23-F30 ◽  
Author(s):  
Alexander A. Denisov ◽  
Olga M. Karpova ◽  
Yuliya S. Korobovtseva ◽  
Konstantin M. Salmakov ◽  
Oleg D. Sklyarov ◽  
...  
Keyword(s):  
Brucella Abortus

scholarly journals Characterization of Three Small Proteins inBrucella abortusLinked to Fucose Utilization

2018 ◽  
Vol 200 (18) ◽  
Author(s):  
James A. Budnick ◽  
Lauren M. Sheehan ◽  
Lin Kang ◽  
Pawel Michalak ◽  
Clayton C. Caswell
Keyword(s):  
Brucella Abortus ◽  
Transcriptional Control ◽  
Sugar Transport ◽  
Genetic System ◽  
Growth Conditions ◽  
Content Type ◽  
Small Regulatory Rna ◽  
Small Proteins

ABSTRACTElucidating the function of proteins <50 amino acids in length is no small task. Nevertheless, small proteins can play vital roles in the lifestyle of bacteria and influence the virulence of pathogens; thus, the investigation of the small proteome is warranted. Recently, our group identified theBrucella abortusprotein VtlR as a transcriptional activator of four genes, one of which is the well-studied small regulatory RNA AbcR2, while the other three genes encode hypothetical small proteins, two of which are highly conserved among the orderRhizobiales. This study provides evidence that all three genes encode authentic small proteins and that all three are highly expressed under oxidative stress, low-pH, and stationary-phase growth conditions. Fractionation of the cells revealed that the proteins are localized to the membranes ofB. abortus. We demonstrate that the small proteins under the transcriptional control of VtlR are not accountable for attenuation observed with theB. abortusvtlRdeletion strain. However, there is an association between VtlR-regulated genes and growth inhibition in the presence of the sugarl-fucose. Subsequent transcriptomic analyses revealed thatB. abortusinitiates the transcription of a locus encoding a putative sugar transport and utilization system when the bacteria are cultured in the presence ofl-fucose. Altogether, our observations characterize the role of the VtlR-controlled small proteins BAB1_0914, BAB2_0512, and BAB2_0574 in the biology ofB. abortus, particularly in the capacity of the bacteria to utilizel-fucose.IMPORTANCEDespite being one of the most common zoonoses worldwide, there is currently no human vaccine to combat brucellosis. Therefore, a better understanding of the pathogenesis and biology ofBrucellaspp., the causative agent of brucellosis, is essential for the discovery of novel therapeutics against these highly infectious bacteria. In this study, we further characterize the virulence-associated transcriptional regulator VtlR inBrucella abortus. Our findings not only shed light on our current understanding of a virulence related genetic system inBrucellaspp. but also increase our knowledge of small proteins in the field of bacteriology.

Characterization of Brucella abortus lipopolysaccharide macrodomains as mega rafts

2006 ◽  
Vol 8 (2) ◽  
pp. 197-206 ◽  
Author(s):  
Nicolas Lapaque ◽  
Frederique Forquet ◽  
Chantal de Chastellier ◽  
Zohair Mishal ◽  
Gilles Jolly ◽  
...  
Keyword(s):  
Brucella Abortus
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