scholarly journals Impact of Copper and Cadmium on the Nutritional Value of the Rotifer Brachionus plicatilis and their Effect on Dicentrarchus labrax Fish Larvae.

2019 ◽  
Vol 23 (2) ◽  
pp. 491-503
Author(s):  
Heba S. El-sayed ◽  
Gihan M. El-khodary
2018 ◽  
Vol 68 (2) ◽  
pp. 181 ◽  
Author(s):  
J. A. THEODOROU

Secondary feeding with commercial lipids enrichments such as the microencapsulated diets Diet A (33.42±3.00%) and Diet B (55.88±3.5%) compared with the marine yeast type product Diet C(8.59±1.0%) as a method of increasing the total lipids and ω-3 PUFA content of rotifers, hence enhancing their nutritional value as live feed prey for fish larvae in hatcheries. The total lipids the rotifers was affected analogous to the levels of these components in the feeds showing its maximum percentage uptake within 4 hours from the enrichment (20.27±3.52, 26.64±3.91, 11.31±2.30 respectively). There was not any significant toxicity to the animals due to the diets during the 16 hours experiment. The DHA/EPA for Diet A(1.31), Diet B(0.42) and Diet C(absent) as well as the DHA/EPA/ARA ratios for Diet A(10.70 ± 1.60 / 8.18 ± 1.10 / 2.08 ± 0.20), Diet B(6.20 ± 2.30 / 14.60 ±1.00 / 1.12 ± 0.40) and Diet C (0 / 5.14 ± 3.40 / 1.30 ± 1.10) indicates that Diet A is closer to the suggested DHA/EPA/ARA optimal value 10/5/1 for marine fish larval growth.


2011 ◽  
Vol 10 (1) ◽  
pp. 67
Author(s):  
Sutia Budi ◽  
. Zainuddin ◽  
Siti Aslamsyah

<p><em>Brachionus</em><em> plicatilis</em> is a widely used as natural feed in hatchery. Characteristics of rotifers as biocapsule can improve the quality of rotifers in a practical way. <em>Bacillus</em><em> </em>sp. is capable of improving the nutrition rotifers to produce vitamins, food detoxication or through enzymatic activity such as protease, lipase, and amylase. This research aimed at observing the influence of the enrichment length of <em>Bacillus </em>sp. on the quality of nutrition and growth of rotifers. The research container was a <em>polyearbonate</em> vessel of one ton volume which contained <em>Nannochloropsis</em> sp. with the density of 10<sup>5</sup> cell/mL, it was then scattered with rotifer with the density of 1,000 ind./mL. The types of bacteria used were <em>Bacillus subtilis</em>, <em>B. pumilus</em> and <em>B. licheformis</em> with the density of 2x10<sup>10</sup> cfu/g. The experiments tried with different enrichment length were A = 0 hour, B = 5 hours, C = 10 hours and D = 10 hours with triplicate. As the comparing data, rotifer culture experiment was carried out with <em>Nannochlorophsis </em>sp.as the control. The number of containers were 24 which consists of 12 for threatment and 12 pieces as control. Variables measured were proximate to each treatment and the growth of rotifers. The result of the research revealed the enrichment length of rotifers with <em>Bacillus</em> sp. has significantly (P<0,05) towards increased protein, crude fiber, ash, BETN, and growth. Optimum enrichment time was obtained at 5 hours.</p> <p>Key words: rotifers, <em>Bacillus</em>, enrichment period, proximate</p> <p> </p> <p>ABSTRAK</p> <p><em>Brachionus plicatilis</em> merupakan pakan alami yang banyak digunakan dalam pembenihan. Karakteristik rotifer sebagai biokapsul secara praktis dapat meningkatkan kualitas rotifer. <em>Bacillus </em>sp<em>.</em> mampu memperbaiki nutrisi rotifer dengan memproduksi vitamin, detoksikasi pangan maupun melalui aktivitas enzimatis seperti <em>protease</em>, <em>lipase</em>, dan <em>amilase</em>. Penelitian ini bertujuan untuk melihat pengaruh pengkayaan <em>Bacillus </em>sp<em>.</em> terhadap kualitas nutrisi dan pertumbuhan rotifer. Wadah penelitian menggunakan bak fiber volume 1 ton yang berisikan <em>Nannochloropsis</em> sp<em>.</em> dengan kepadatan 10<sup>5</sup> cell/mL, kemudian ditebari rotifer dengan kepadatan 1.000 ind./mL. Jenis bakteri yang digunakan berupa <em>Bacillus</em> <em>subtilis</em>, <em>B. Pumilus </em>dan<em> B. Licheformis </em>dengan kepadatan 2x10<sup>10</sup> cfu/g. Perlakuan yang diujikan adalah lama pengkayaan probiotik <em>Bacillus </em>sp<em>.</em> yang berbeda, yaitu perlakuan A (0 jam),  perlakuan B (5 Jam), perlakuan C (10 jam) dan perlakuan D (15 jam). Sebagai data pembanding dilakukan percobaan kultur rotifer dengan <em>Nannochloropsis</em> sp<em>.</em> sebagai kontrol. Jumlah wadah penelitian yang dipergunakan sebanyak 24 buah yang terdiri atas 12 buah untuk perlakuan dan 12 buah sebagai kontrol. Peubah yang diukur adalah proksimat pada masing-masing perlakuan dan pertumbuhan rotifer.  Hasil penelitian menunjukkan bahwa perlakuan lama pengkayaan rotifer dengan <em>Bacillus</em> sp<em>.</em> meningkatkan (P<0,05) terhadap kadar protein, serat kasar, abu, BETN, dan pertumbuhan rotifer. Lama pengkayaan optimum diperoleh pada 5 jam.</p> <p>Kata kunci: rotifer, <em>Bacillus</em>, lama pengkayaan, proksimat</p>


2020 ◽  
Vol 20 (2) ◽  
pp. 81-89
Author(s):  
Mahmouud Hegab ◽  
Moustafa Sobhy Abdelhameed ◽  
Hoda Shafiek Nasr ◽  
Hesham Abdel Mola

The study tested a new feeding protocol to find out the best program using cheap and available products to enhancement the mass culture and the nutritional value of B. plicatilis. The feeding regime was six artificial treatments formulated from dried yeast (Saccharomyces cervisiae) with sucrose sugar and yeast with molasses in different concentrations. Additionally, a live Cyclotella sp. had been applied as supplementary food to each treatment and as independent treatment (control). B. plicatilis was cultured in small scale to find the best food regime and technique to apply it in the large one. B. plicatilis attained its highest density in the small scale culture (370 Ind.ml1 ) with T1 (30 % yeast, 70 % sugar and Cyclotella sp.) at the 12th day, while the highest population growth rate (PGR) (0.65) was calculated at the 9th day. According to the analysis of variance (ANOVA) and PGR, T1 had been applied in the large scale culture. B. plicatilis samples were composed of 51.6% total protein with 16 amino acids and 33.01% total lipid with 19 identified fatty acids. The study concluded that the treatment (T1) is a suitable diet for enrichment the mass culture and the nutritional value of B. plicatilis.


Aquaculture ◽  
1993 ◽  
Vol 112 (2-3) ◽  
pp. 195-206 ◽  
Author(s):  
M.J. Fernández-Reiriz ◽  
U. Labarta ◽  
M.J. Ferreiro

Aquaculture ◽  
2006 ◽  
Vol 253 (1-4) ◽  
pp. 421-432 ◽  
Author(s):  
Nguyen Thi Ngoc Tinh ◽  
Nguyen Ngoc Phuoc ◽  
Kristof Dierckens ◽  
Patrick Sorgeloos ◽  
Peter Bossier

Author(s):  
Swagat Ghosh ◽  
T. T. Ajith Kumar ◽  
Bandana Das Ghosh ◽  
N. C. Sahu

The present study, evaluated the effect of enrichment of the rotifer Brachionus plicatilis with the microalga Nannochloroposis salina, amino acid derivative L-carnitine and commercially available S-presso (SELCO). The study also evaluated the effect of enriched rotifers in larval rearing of Amphiprion clarkii in terms of metamorphosis, pigmentation, growth and survival of larvae.. Rotifer production increased by 43, 39 and 54% respectively in B. plicatilis enriched with different concentrations of L-carnitine at 1, 10 and 100 mg l-1 on day 4 (p<0.001) in comparison with the control group maintained on algal diet alone. Total unsaturated fatty acid (PUFA, HUFA and n-3 fatty acids) and the ratios of n-3/ n-6, DHA/EPA, EPA/AA were significantly higher (p<0.05) in the L-carnitine treated rotifers compared with the S-presso enriched as well N. salina enriched groups. Larvae of A. clarkii, fed with L-carnitine enriched rotifer achieved maximum growth of 56.92±0.2 mg; whereas, those fed S-presso treated rotifers attained only 48.74±1 mg. In L-carnitine enrichment; metamorphosis of clown fish larvae took only 10 days, while it took 11 days and 10 days respectively in S-presso and N. salina enrichment. In L-carnitine and S-presso enrichments, initial pigmentation of larvae was observed within first 3-4 days of culture, whereas it took 4-5 days in N. salina enrichment. L-carnitine enrichment resulted in the highest survival of 70%, followed by N. salina with 68% and S-presso enriched group with 52%. The results indicate that L-carnitine could be a recommendable enrichment for increasing rotifer production as well as for clown fish larval culture.


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