scholarly journals Nutritional enrichment of rotifer Brachionus plicatilis and its effect on clownfish larvae Amphiprion clarkii (Bennett, 1830)

2017 ◽  
Vol 64 ◽  
Author(s):  
Swagat Ghosh ◽  
T. T. Ajith Kumar ◽  
Bandana Das Ghosh ◽  
N. C. Sahu
Keyword(s):  
Brachionus Plicatilis ◽  
Fish Larvae ◽  
Maximum Growth ◽  
Amino Acid Derivative ◽  
Control Group ◽  
Larval Rearing ◽  
Growth And Survival ◽  
Rotifer Brachionus Plicatilis ◽  
Total Unsaturated Fatty Acid ◽  
Amphiprion Clarkii

The present study, evaluated the effect of enrichment of the rotifer Brachionus plicatilis with the microalga Nannochloroposis salina, amino acid derivative L-carnitine and commercially available S-presso (SELCO). The study also evaluated the effect of enriched rotifers in larval rearing of Amphiprion clarkii in terms of metamorphosis, pigmentation, growth and survival of larvae.. Rotifer production increased by 43, 39 and 54% respectively in B. plicatilis enriched with different concentrations of L-carnitine at 1, 10 and 100 mg l-1 on day 4 (p<0.001) in comparison with the control group maintained on algal diet alone. Total unsaturated fatty acid (PUFA, HUFA and n-3 fatty acids) and the ratios of n-3/ n-6, DHA/EPA, EPA/AA were significantly higher (p<0.05) in the L-carnitine treated rotifers compared with the S-presso enriched as well N. salina enriched groups. Larvae of A. clarkii, fed with L-carnitine enriched rotifer achieved maximum growth of 56.92±0.2 mg; whereas, those fed S-presso treated rotifers attained only 48.74±1 mg. In L-carnitine enrichment; metamorphosis of clown fish larvae took only 10 days, while it took 11 days and 10 days respectively in S-presso and N. salina enrichment. In L-carnitine and S-presso enrichments, initial pigmentation of larvae was observed within first 3-4 days of culture, whereas it took 4-5 days in N. salina enrichment. L-carnitine enrichment resulted in the highest survival of 70%, followed by N. salina with 68% and S-presso enriched group with 52%. The results indicate that L-carnitine could be a recommendable enrichment for increasing rotifer production as well as for clown fish larval culture.

scholarly journals Pengaruh ketinggian air dalam pemeliharaan larva ikan hias botia (Chromobotia macracanthus, Bleeker)

2014 ◽  
Vol 1 (1) ◽  
pp. 24 ◽  
Author(s):  
Maulina Sari ◽  
Muhammad Hatta ◽  
Asep Permana
Keyword(s):  
Weight Gain ◽  
Survival Rate ◽  
Water Level ◽  
Fish Larvae ◽  
Quality Parameters ◽  
Water Levels ◽  
Average Weight ◽  
Larval Rearing ◽  
Growth And Survival ◽  
Average Value

Penelitian ini bertujuan untuk mengetahui pengaruh ketinggian air yang berbeda terhadap pertumbuhan dan kelangsungan hidup larva ikan botia (Chromobotia macracanthus, Bleeker). Hasil penelitian menunjukkan bahwa sintasan atau kelangsungan hidup tertinggi terdapat pada perlakuan B dengan ketinggian air 10 cm yaitu sebesar 98.88%, sedangkan kelangsungan hidup terendah terdapat pada perlakuan A dengan ketinggian air 5 cm yaitu sebesar 97.99%. Pertambahan bobot nilai tertinggi terjadi pada perlakuan A dengan ketinggian air 5 cm dengan nilai bobot rata–rata 0.02252 gr dan pertambahan bobot rata–rata terendah terjadi pada perlakuan C dengan ketinggian air 15 cm yaitu sebesar 0.01132 gr. Pertambahan panjang rata–rata tertinggi terjadi pada perlakuan A yaitu sebesar 0.42 cm dan yang terendah terjadi pada perlakuan B yaitu sebesar 0.29 cm. Parameter kualitas air selama penelitian masih dalam kisaran optimal dimana suhu 24,3–28,9oC, pH 6,5–7,0, DO 6,84–7,69 ppm, NH3 0,00–0,03 ppm, NO2 0,00–0,07 ppm. Analisis statistik dengan uji F diperoleh bahwa ketinggian air yang berbeda dalam pemeliharaan larva ikan botia (C. macracanthus, Bleeker) berbeda sangat nyata (P>0.01) terhadap pertambahan bobot dengan nilai Fhitung (21.00) > Ftabel (10.92), dan berbeda sangat nyata terhadap pertambahan panjang dengan nilai Fhitung (23.56) > Ftabel (10.92), sedangkan untuk sintasan atau kelangsungan hidup tidak berbeda nyata antar perlakuan. Hasil uji BNT menunjukkan, setiap perlakuan memberikan pengaruh terhadap pertumbuhan dan tidak berpengaruh terhadap kelangsungan hidup.The study aims to determine the effect of different water levels on the growth and survival of fish larvae Botia (Chromobotia macracanthus, Bleeker). The results showed that the survival rate was highest in treatment B with the water level was 10 cm which was equal to 98.88 %, whereas the lowest survival rate was found in treatment A with the water level was 5 cm which was equal to 97.99 %. The highest value of weight gain occurred in treatment A with average value in weight was 0.02252 g and the average weight gain was lowest in the treatment of C that reached 0.01132 g. The highest growth of length was occured in treatment A that was equal to 0.42 cm and the lowest occurred in treatment B that was 0.29 cm. Water quality parameters during the study were in the tolerance range where the optimum temperature were 24,3-28,9 oC , pH 6.5 to 7.0, DO 6.84 to 7.69 ppm, NH3 0.00 to 0.03 ppm , NO2 0.00 to 0.07 ppm . Statistical analysis by F test showed that the different water levels in the larval rearing of fish Botia (C. macracanthus, Bleeker) was significantly different ( P > 0.01) in the weight gain with the value of Fcount ( 21.00 ) > Ftable ( 10.92 ), and it was significantly different against the length with the value of F count ( 23:56 ) > F table ( 10.92), while for the survival rate showed has not significantly different between treatments. The LSD test showed that all the treatment were effected the growth rate, but not for survival rate.

scholarly journals Total lipids content and fatty acids composition of the rotifer Brachionus plicatilis using artificial enrichments

2018 ◽  
Vol 68 (2) ◽  
pp. 181 ◽  
Author(s):  
J. A. THEODOROU
Keyword(s):  
Nutritional Value ◽  
Brachionus Plicatilis ◽  
Fish Larvae ◽  
Larval Growth ◽  
Total Lipids ◽  
Pufa Content ◽  
Live Feed ◽  
Significant Toxicity ◽  
Optimal Value ◽  
Rotifer Brachionus Plicatilis

Secondary feeding with commercial lipids enrichments such as the microencapsulated diets Diet A (33.42±3.00%) and Diet B (55.88±3.5%) compared with the marine yeast type product Diet C(8.59±1.0%) as a method of increasing the total lipids and ω-3 PUFA content of rotifers, hence enhancing their nutritional value as live feed prey for fish larvae in hatcheries. The total lipids the rotifers was affected analogous to the levels of these components in the feeds showing its maximum percentage uptake within 4 hours from the enrichment (20.27±3.52, 26.64±3.91, 11.31±2.30 respectively). There was not any significant toxicity to the animals due to the diets during the 16 hours experiment. The DHA/EPA for Diet A(1.31), Diet B(0.42) and Diet C(absent) as well as the DHA/EPA/ARA ratios for Diet A(10.70 ± 1.60 / 8.18 ± 1.10 / 2.08 ± 0.20), Diet B(6.20 ± 2.30 / 14.60 ±1.00 / 1.12 ± 0.40) and Diet C (0 / 5.14 ± 3.40 / 1.30 ± 1.10) indicates that Diet A is closer to the suggested DHA/EPA/ARA optimal value 10/5/1 for marine fish larval growth.

scholarly journals Use of commercial probiotics for the improvement of water quality and rotifer density in outdoor mass culture tanks

2016 ◽  
Vol 63 (4) ◽  
Author(s):  
Jayasree Loka ◽  
S. M. Sonali ◽  
Purbali Saha ◽  
K. Devaraj ◽  
K. K. Philipose
Keyword(s):  
Mass Culture ◽  
Brachionus Plicatilis ◽  
Nitrifying Bacteria ◽  
Control Group ◽  
Probiotic Treatment ◽  
Rotifer Brachionus Plicatilis ◽  
Bacillus Spp ◽  
Outdoor Mass Culture ◽  
Commercial Probiotics ◽  
Total Elimination

An experimental study was carried out to evaluate the efficacy of three commercially available probiotics P1, P2 and P3 consisting of mainly Bacillus spp. and nitrifying bacteria against Vibrio loads in mass culture tanks of the rotifer Brachionus plicatilis. Triplicate tanks were maintained for each of the probiotic treatment as well as for control group. All the tanks were inoculated with 50 rotifers ml-1 and were fed with Nannochloropsis oculata at a density of 1 x 107 cells ml-1. Every alternate day, all the experimental tanks were treated with probiotics at a concentration of 1 x 104 cfu ml-1 and the experiment was carried out for one week. The study showed a significant increase in rotifer density (p<0.05) in all the tanks treated with the probiotics and a maximum density of 400 nos. ml-1 was observed in the tanks treated with P3. After 5th and 6th day of culture, total elimination of Vibrios was also recorded in the tanks treated with P3 and P2 respectively. The study revealed that P3, with a combination of Bacillus, Thiobacillus, Acetobacter and Paracoccus supplemented with enzymes, was found to be most effective in the enhancement of rotifer density and also in the elimination of Vibrios in rotifer mass culture tanks.

scholarly journals PENGARUH PERBEDAAN PADAT TEBAR LARVA TERHADAP PERTUMBUHAN DAN SINTASAN PADA IKAN UCENG (Nemacheilus fasciatus)

2019 ◽  
Vol 18 (2) ◽  
pp. 209-214
Author(s):  
Jojo Subagja ◽  
Vitas Atmadi Prakoso ◽  
Otong Zenal Arifin ◽  
Anang Hari Kristanto
Keyword(s):  
Weight Gain ◽  
Survival Rate ◽  
Specific Growth ◽  
Negative Impact ◽  
Fish Larvae ◽  
Survival Rates ◽  
Stocking Density ◽  
Larval Rearing ◽  
Growth And Survival ◽  
Length Gain

Proper stocking density on larval rearing of barred loach (Nemacheilus fasciatus Valenciennes, 1846) is very important in terms of obtaining the best growth and survival rate. This study was aimed to observe the growth and survival rate of barred loach subjected to different larval stocking density. Fish larvae were obtained by artificial spawning from in aquarium. Larvae were stocked on aquariums (50 × 40 × 30 cm) with three stocking density treatments (5, 10, and 15 individuals/L) and three replications. The experimental fish larvae were fed by Artemia and commercial feed (crude protein content: 30%) and feeding frequency twice per day (ad libitum). The length and weight were reassured every 10 days during 70 days of rearing period. Temperature, pH, and dissolved oxygen were measured at each treatment. Data obtained from measurements were analysed to assess the length gain, weight gain, specific growth rate (SGR), biomass gain, and survival rate. The results showed length gain of 3.35 ± 0.01, 2.94 ± 0.10, and 2.82 ± 0.15 cm and weight gain of 0.573 ± 0.023, 0.436 ± 0.018, and 0.417 ± 0.043 g in larval stocking density of 5, 10 and 15 individuals/L, respectively. Specific growth rates and biomass growth were of 6.22 ± 0.41, 5.93 ± 0.21, and 5.68 ± 0.41 %/day and 94.04 ± 5.39, 146.80 ± 3.81, and 167.24 ± 17.48 g in larval stocking density of 5, 10 and 15 individuals/L, respectively. Meanwhile, the survival rates obtained in this study were 89.13 ± 7.25, 93.13 ± 5.13, and 76.50 ± 3.54 % in larval stocking density of 5, 10 and 15 individuals/L, respectively. Increased stocking density has a negative impact on the growth and survival rate of barred loach.

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