Total lipids content and fatty acids composition of the rotifer Brachionus plicatilis using artificial enrichments

Secondary feeding with commercial lipids enrichments such as the microencapsulated diets Diet A (33.42±3.00%) and Diet B (55.88±3.5%) compared with the marine yeast type product Diet C(8.59±1.0%) as a method of increasing the total lipids and ω-3 PUFA content of rotifers, hence enhancing their nutritional value as live feed prey for fish larvae in hatcheries. The total lipids the rotifers was affected analogous to the levels of these components in the feeds showing its maximum percentage uptake within 4 hours from the enrichment (20.27±3.52, 26.64±3.91, 11.31±2.30 respectively). There was not any significant toxicity to the animals due to the diets during the 16 hours experiment. The DHA/EPA for Diet A(1.31), Diet B(0.42) and Diet C(absent) as well as the DHA/EPA/ARA ratios for Diet A(10.70 ± 1.60 / 8.18 ± 1.10 / 2.08 ± 0.20), Diet B(6.20 ± 2.30 / 14.60 ±1.00 / 1.12 ± 0.40) and Diet C (0 / 5.14 ± 3.40 / 1.30 ± 1.10) indicates that Diet A is closer to the suggested DHA/EPA/ARA optimal value 10/5/1 for marine fish larval growth.

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Impact of Copper and Cadmium on the Nutritional Value of the Rotifer Brachionus plicatilis and their Effect on Dicentrarchus labrax Fish Larvae.

Egyptian Journal of Aquatic Biology and Fisheries ◽

10.21608/ejabf.2019.33919 ◽

2019 ◽

Vol 23 (2) ◽

pp. 491-503

Author(s):

Heba S. El-sayed ◽

Gihan M. El-khodary

Keyword(s):

Nutritional Value ◽

Brachionus Plicatilis ◽

Fish Larvae ◽

Dicentrarchus Labrax ◽

Rotifer Brachionus Plicatilis

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Nutritional value and production performance of the rotifer Brachionus plicatilis Müller, 1786 cultured with different feeds at commercial scale

Aquaculture International ◽

10.1007/s10499-019-00375-5 ◽

2019 ◽

Vol 27 (3) ◽

pp. 875-890 ◽

Cited By ~ 6

Author(s):

Kamil Mert Eryalçın

Keyword(s):

Nutritional Value ◽

Brachionus Plicatilis ◽

Production Performance ◽

Commercial Scale ◽

Rotifer Brachionus Plicatilis

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Study on physiological function of the rotifer Brachionus plicatilis sp. complex and its dietary effects for fish larvae

NIPPON SUISAN GAKKAISHI ◽

10.2331/suisan.73.433 ◽

2007 ◽

Vol 73 (3) ◽

pp. 433-436 ◽

Cited By ~ 2

Author(s):

ATSUSHI HAGIWARA

Keyword(s):

Brachionus Plicatilis ◽

Physiological Function ◽

Fish Larvae ◽

Dietary Effects ◽

Rotifer Brachionus Plicatilis

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Stable mass production technique and quality as live feed of the euryhaline rotifer Brachionus plicatilis

NIPPON SUISAN GAKKAISHI ◽

10.2331/suisan.72.259 ◽

2006 ◽

Vol 72 (2) ◽

pp. 259-262 ◽

Cited By ~ 1

Author(s):

MASAHIKO KOISO

Keyword(s):

Mass Production ◽

Brachionus Plicatilis ◽

Production Technique ◽

Live Feed ◽

Rotifer Brachionus Plicatilis

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Improvement of the nutritional value and growth of rotifer (Brachionus plicatilis) by different enrichment period with Bacillus sp.

Jurnal Akuakultur Indonesia ◽

10.19027/jai.10.67-73 ◽

2011 ◽

Vol 10 (1) ◽

pp. 67

Author(s):

Sutia Budi ◽

. Zainuddin ◽

Siti Aslamsyah

Keyword(s):

Bacillus Subtilis ◽

Enzymatic Activity ◽

Nutritional Value ◽

Brachionus Plicatilis ◽

Bacillus Sp ◽

Fiber Volume ◽

Culture Experiment ◽

Growth Optimum ◽

Rotifer Brachionus Plicatilis

Brachionus plicatilis is a widely used as natural feed in hatchery. Characteristics of rotifers as biocapsule can improve the quality of rotifers in a practical way. Bacillus sp. is capable of improving the nutrition rotifers to produce vitamins, food detoxication or through enzymatic activity such as protease, lipase, and amylase. This research aimed at observing the influence of the enrichment length of Bacillus sp. on the quality of nutrition and growth of rotifers. The research container was a polyearbonate vessel of one ton volume which contained Nannochloropsis sp. with the density of 105 cell/mL, it was then scattered with rotifer with the density of 1,000 ind./mL. The types of bacteria used were Bacillus subtilis, B. pumilus and B. licheformis with the density of 2x1010 cfu/g. The experiments tried with different enrichment length were A = 0 hour, B = 5 hours, C = 10 hours and D = 10 hours with triplicate. As the comparing data, rotifer culture experiment was carried out with Nannochlorophsis sp.as the control. The number of containers were 24 which consists of 12 for threatment and 12 pieces as control. Variables measured were proximate to each treatment and the growth of rotifers. The result of the research revealed the enrichment length of rotifers with Bacillus sp. has significantly (P<0,05) towards increased protein, crude fiber, ash, BETN, and growth. Optimum enrichment time was obtained at 5 hours. Key words: rotifers, Bacillus, enrichment period, proximate ABSTRAK Brachionus plicatilis merupakan pakan alami yang banyak digunakan dalam pembenihan. Karakteristik rotifer sebagai biokapsul secara praktis dapat meningkatkan kualitas rotifer. Bacillus sp. mampu memperbaiki nutrisi rotifer dengan memproduksi vitamin, detoksikasi pangan maupun melalui aktivitas enzimatis seperti protease, lipase, dan amilase. Penelitian ini bertujuan untuk melihat pengaruh pengkayaan Bacillus sp. terhadap kualitas nutrisi dan pertumbuhan rotifer. Wadah penelitian menggunakan bak fiber volume 1 ton yang berisikan Nannochloropsis sp. dengan kepadatan 105 cell/mL, kemudian ditebari rotifer dengan kepadatan 1.000 ind./mL. Jenis bakteri yang digunakan berupa Bacillus subtilis, B. Pumilus dan B. Licheformis dengan kepadatan 2x1010 cfu/g. Perlakuan yang diujikan adalah lama pengkayaan probiotik Bacillus sp. yang berbeda, yaitu perlakuan A (0 jam), perlakuan B (5 Jam), perlakuan C (10 jam) dan perlakuan D (15 jam). Sebagai data pembanding dilakukan percobaan kultur rotifer dengan Nannochloropsis sp. sebagai kontrol. Jumlah wadah penelitian yang dipergunakan sebanyak 24 buah yang terdiri atas 12 buah untuk perlakuan dan 12 buah sebagai kontrol. Peubah yang diukur adalah proksimat pada masing-masing perlakuan dan pertumbuhan rotifer. Hasil penelitian menunjukkan bahwa perlakuan lama pengkayaan rotifer dengan Bacillus sp. meningkatkan (P<0,05) terhadap kadar protein, serat kasar, abu, BETN, dan pertumbuhan rotifer. Lama pengkayaan optimum diperoleh pada 5 jam. Kata kunci: rotifer, Bacillus, lama pengkayaan, proksimat

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The influence of diet on the microbiota of live-feed rotifers (Brachionus plicatilis) used in commercial fish larviculture

FEMS Microbiology Letters ◽

10.1093/femsle/fnaa020 ◽

2020 ◽

Vol 367 (2) ◽

Cited By ~ 2

Author(s):

Emre Turgay ◽

Terje Marken Steinum ◽

Kamil Mert Eryalçın ◽

Remziye Eda Yardımcı ◽

Süheyla Karataş

Keyword(s):

Brachionus Plicatilis ◽

Fish Larvae ◽

Bacterial Species ◽

Alpha Diversity ◽

Amplicon Sequencing ◽

Rrna Gene ◽

Fish Pathogens ◽

Study Objective ◽

Commercial Fish ◽

Live Feed

ABSTRACT Live-feed is indispensable to commercial fish larviculture. However, high bacterial loads in rotifers could pose a biosecurity risk. While this may be true, live-feed associated bacteria could also be beneficial to fish larvae through improved feed utilization or pathogen inhibition following host microbiota modification. The study objective was to elucidate the largely unexplored microbiota of rotifers propagated on five different diets through bacterial community profiling by 16S rRNA gene amplicon sequencing. Investigated rotifer samples had a median observed alpha-diversity of 338 ± 87 bacterial species. Alpha- and Gamma-Proteobacteria dominated the rotifer microbiota followed by members of classes Flavobacteriia, Cytophagia, Mollicutes, Phycisphaerae and Bacteroidia. Different diets significantly altered the bacterial communities associated with rotifers according to PERMANOVA test results and beta dispersion calculations. A common core rotifer microbiome included 31 bacterial species present in relative abundances over 0.01%. We discuss the functional role of some microbiome members. Our data suggested the presence of several known fish pathogens in stock rotifers. However, we found no evidence for increased loads of these presumptive taxa in propagated live-feed rotifers during this field trial.

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Applying a New Feeding Protocol for Enhancing Mass Culture and Nutritional Value of the Rotifer Brachionus plicatilis Müller, 1786

Aquaculture Studies ◽

10.4194/2618-6381-v20_2_02 ◽

2020 ◽

Vol 20 (2) ◽

pp. 81-89

Author(s):

Mahmouud Hegab ◽

Moustafa Sobhy Abdelhameed ◽

Hoda Shafiek Nasr ◽

Hesham Abdel Mola

Keyword(s):

Mass Culture ◽

Nutritional Value ◽

Large Scale ◽

Brachionus Plicatilis ◽

Small Scale ◽

Food Regime ◽

Treatment Control ◽

Rotifer Brachionus Plicatilis ◽

Large Scale Culture ◽

Feeding Protocol

The study tested a new feeding protocol to find out the best program using cheap and available products to enhancement the mass culture and the nutritional value of B. plicatilis. The feeding regime was six artificial treatments formulated from dried yeast (Saccharomyces cervisiae) with sucrose sugar and yeast with molasses in different concentrations. Additionally, a live Cyclotella sp. had been applied as supplementary food to each treatment and as independent treatment (control). B. plicatilis was cultured in small scale to find the best food regime and technique to apply it in the large one. B. plicatilis attained its highest density in the small scale culture (370 Ind.ml1 ) with T1 (30 % yeast, 70 % sugar and Cyclotella sp.) at the 12th day, while the highest population growth rate (PGR) (0.65) was calculated at the 9th day. According to the analysis of variance (ANOVA) and PGR, T1 had been applied in the large scale culture. B. plicatilis samples were composed of 51.6% total protein with 16 amino acids and 33.01% total lipid with 19 identified fatty acids. The study concluded that the treatment (T1) is a suitable diet for enrichment the mass culture and the nutritional value of B. plicatilis.

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Live Feeds Used in the Larval Culture of Red Cusk Eel, Genypterus chilensis, Carry High Levels of Antimicrobial-Resistant Bacteria and Antibiotic-Resistance Genes (ARGs)

Animals ◽

10.3390/ani10030505 ◽

2020 ◽

Vol 10 (3) ◽

pp. 505

Author(s):

Luz Hurtado ◽

Claudio D. Miranda ◽

Rodrigo Rojas ◽

Félix A. Godoy ◽

Mark A. Añazco ◽

...

Keyword(s):

Fish Larvae ◽

Larval Growth ◽

Survival Rates ◽

Larval Survival ◽

Resistant Bacteria ◽

Rrna Gene ◽

Live Feed ◽

Multiresistant Bacteria ◽

A Priority ◽

Genypterus Chilensis

The culture of red cusk eel Genypterus chilensis is currently considered a priority for Chilean aquaculture but low larval survival rates have prompted the need for the continuous use of antibacterials. The main aim of this study was to evaluate the role of live feed as a source of antibacterial-resistant bacteria in a commercial culture of G. chilensis. Samples of rotifer and Artemia cultures used as live feed were collected during the larval growth period and culturable bacterial counts were performed using a spread plate method. Rotifer and Artemia cultures exhibited high levels of resistant bacteria (8.03 × 104 to 1.79 × 107 CFU/g and 1.47 × 106 to 3.50 × 108 CFU/g, respectively). Sixty-five florfenicol-resistant isolates were identified as Vibrio (81.5%) and Pseudoalteromonas (15.4%) using 16S rRNA gene sequence analysis. A high incidence of resistance to streptomycin (93.8%), oxytetracycline (89.2%), co-trimoxazole (84.6%), and kanamycin (73.8%) was exhibited by resistant isolates. A high proportion of isolates (76.9%) carried the florfenicol-resistance encoding genes floR and fexA, as well as plasmid DNA (75.0%). The high prevalence of multiresistant bacteria in live feed increases the incidence of the resistant microbiota in reared fish larvae, thus proper monitoring and management strategies for live feed cultures appear to be a priority for preventing future therapy failures in fish larval cultures.

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